High-Yield Production of PCV2 Cap Protein: Baculovirus Vector Construction and Cultivation Process Optimization.

Porcine circovirus type 2 (PCV2) infection causes porcine circovirus disease (PCVD), a global immunosuppressive disease in pigs. Its clinical manifestations include post-weaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS), which cause significant economic losses to the swine industry. The Cap protein, which is the major protective antigen of PCV2, can self-assemble to form virus-like particles (VLPs) in the insect baculovirus expression system.

Advanced imaging-enabled understanding of cell wall remodeling mechanisms mediating plant drought stress tolerance.

Drought stress causes peculiar challenges to plant cells reliant on turgor pressure and a polysaccharides-enriched cell wall for growth and development. Appropriate cell wall changes in mechanical properties and biochemical composition under stress conditions constitute an indispensable stress adaptation strategy. A better understanding of stress-induced cell wall modifications is not only crucial for accruing fundamental scientific knowledge in plant biology, but will help us design novel strategies for enhancing crop drought tolerance.

Precision-Engineered Co-N -C Single Atoms Enhance Potential-Resolved Ru(bpy) Electrochemiluminescence via Reactive Oxygen Species.

Electrochemiluminescence (ECL) immunoassays based on tris(bipyridine)ruthenium [Ru(bpy) ] is the luminophore representing the most advanced and widely adopted approach in the field of in vitro diagnostics (IVD). However, the scarcity of potential-resolved ECL promoters for Ru(bpy) markedly limits its application in clinical diagnostics. Here, we report the first application of cobalt single-atom catalysts (SACs) designed via density functional theory (DFT) calculations to boost the multi-signal ECL of Ru(bpy) .

Erratum: Pulsed magnetic field promotes proliferation and neurotrophic genes expression in Schwann cells .

[This corrects the article on p. 2343 in vol. 8, PMID: 26045741.

Clinical and Cost-Effectiveness of Telehealth-Supported Home Oxygen Therapy on Adherence, Hospital Readmission, and Health-Related Quality of Life in Patients With Chronic Obstructive Pulmonary Disease: Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Background: Chronic obstructive pulmonary disease (COPD) is a common respiratory disorder frequently requiring oxygen therapy to relieve symptoms and improve survival. In recent years, telehealth-supported interventions have emerged as promising strategies to optimize home oxygen therapy by promoting adherence, reducing hospitalizations, and enhancing health-related quality of life. However, evidence regarding their effectiveness remains inconsistent and equivocal, underscoring the need for further rigorous evaluation.

Uncovering the Efficacy and Mechanism of Zhenwu Decoction for Hypothyroidism Based on Non-Targeted Metabolomics.

Background: Hypothyroidism, a systemic hypometabolic syndrome from deficient thyroid hormone synthesis/utilization, has high prevalence and complex mechanisms. Levothyroxine-based lifelong therapy risks neurocognitive/metabolic complications and iatrogenic pathologies (eg, osteoporosis, hepatocardiac dysfunction). Zhenwu Decoction (ZW), a classical multi-target herbal formulation, shows therapeutic potential but requires mechanistic clarity on thyroid restoration to enable standardized translation.

Magnesium-Free Assembly of Cationic Peptide/DNA Nanostructures with Defined Geometries for Anticancer Drug Delivery.

DNA nanostructures are extensively utilized in synthetic biology, biosensing, bioimaging, and therapeutic delivery because of their programmability and biocompatibility. Although DNA nanostructure-based drug delivery systems have shown significant potential, the clinical application of nucleic acid drugs has been hindered by their biological instability and low delivery efficiency. This study proposes utilizing a cationic targeting peptide by combining a cell-penetrating peptide (TAT) with a cancer cell-targeting peptide (RGD) instead of conventional magnesium ions to facilitate the self-assembly of defined DNA nanostructures.

Comprehensive Analysis of Shear Deformation Cytometry Based on Numerical Simulation Method.

The deformability of cells reflects their capacity for shape changes under external forces; however, the systematic investigation of deformation-influencing factors remains conspicuously underdeveloped. In this work, by using an incompressible neo-Hookean viscoelastic solid model, coupled with the Kelvin-Voigt model, the effects of flow rate, fluid viscosity, cell diameter, and shear modulus on cell deformability were systematically calculated and simulated. Additionally, the relationship between cell deformability and relaxation time within a dissipative process was also simulated.

Dissecting the causal role of immunophenotypes in brain meningioma risk: A Mendelian randomization study.

Meningiomas are primarily benign brain tumors that, despite their typically slow growth, often cause significant health issues due to their location. Emerging immunotherapy approaches have sparked optimism regarding treatment outcomes for patients. We conducted a comprehensive Mendelian randomization (MR) analysis, utilizing 731 immune cell phenotypes to explore causal relationships with Brain Meningioma.

Efficacy of Motion-Sensing Game-Assisted Pulmonary Rehabilitation in Patients With Chronic Obstructive Pulmonary Disease: Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Background: While motion-sensing game (MSG) platforms provide immersive, real-time feedback environments for rehabilitation, research findings on their effectiveness in chronic obstructive pulmonary disease (COPD) pulmonary rehabilitation remain heterogeneous.

Objective: This study aims to systematically evaluate the efficacy of MSG-assisted pulmonary rehabilitation for patients with COPD.

Methods: This meta-analysis was conducted in accordance with the Cochrane Handbook for Systematic Reviews of Interventions and the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) 2020 statement.

An innovative electrophoresis-coupled electrochemiluminescence immunosensor for rapid and sensitive detection of carcinoembryonic antigen.

Electrochemiluminescence (ECL) immunosensor provides unique advantages for the sensitive biomarker detection. However, the lengthy detection duration and plentiful system interferences have hindered their development. Here, we present an innovative approach in which electrophoresis coupled with ECL immunoassay was implemented to construct an electric field-enhanced ECL immunosensor for efficient detection of carcinoembryonic antigen (CEA).

Hydroenergy Inspiring Large-Scale Piezoelectric Catalysis for Seawater Hydrogen Evolution.

The piezoelectric effect presents a promising avenue for harvesting and utilizing low-grade mechanical energy. However, challenges from lacking matched industrial equipment and maintaining catalytic performance during scaling up impede the engineering of piezo-catalysis. Here, utilizing mechanical energy of water to inspire piezoelectricity, a successful attempt at large-scale piezo-catalysis seawater H evolution was reported for the first time, exhibiting an efficiency of 36.

Intramolecular Switching Oxygen Reduction Reaction Driven by Dual-Crystalline Bimetallic MOF induces Self-Potential-Resolved Electrochemiluminescence Biosensor.

Framework materials, particularly metal-organic frameworks (MOFs), have emerged as versatile platforms for multifunctional applications due to their tunable structures and properties. In this study, a series of bimetallic ZnRuMOFs with dual-crystalline phase structure was designed and synthesized. This unique dual-crystalline bimetallic MOF configuration enables intramolecular autocatalysis and selective oxygen reduction reaction (ORR).

Oral Delivery of Expressing Full-Length S Protein via Alginate-Chitosan Capsules Induces Immune Protection Against PEDV Infection in Mice.

: Porcine epidemic diarrhea (PED) is a highly contagious enteric infectious disease that causes severe morbidity and mortality in piglets, posing significant economic losses to the swine industry worldwide. Oral vaccines based on offer a promising approach due to their safety and genetic manipulability. This study aims to develop and evaluate an oral -based vaccine expressing the full-length PEDV S protein.

A potent protective bispecific nanobody targeting Herpes simplex virus gD reveals vulnerable epitope for neutralizing.

Herpes simplex virus (HSV) causes significant health burden worldwide. Currently used antiviral drugs are effective but resistance can occur. Here, we report two high-affinity neutralizing nanobodies, namely Nb14 and Nb32, that target non-overlapping epitopes in HSV gD.

[Spatial Distribution, Influencing Factors, Risk Assessment, and Source Analysis of Heavy Metals in Soil Profile of Farmland in the Guanzhong Plain].

In order to understand the spatial distribution, influencing factors, ecological risk characteristics, and sources of heavy metals in the soil profile （0-1 m） of farmland in the Guanzhong Plain, soil samples of 0-1 m were collected from 124 sites in the Guanzhong Plain. The contents of heavy metals （As, Cd, Cr, Cu, Ni, Pb, and Zn） in soil were analyzed at different depths （0-10 cm, 10-20 cm, 20-40 cm, 40-60 cm, and 60-100 cm）. The study generated the spatial distribution characteristics of soil heavy metals based on geostatistics and investigated the influencing factors of heavy metals based on structural equation modeling （SEM）.

Mechanical Stretching Induced Highly Tunable and Reversible Mid-infrared Plasmonic Resonances in a Conductive Polymer Thin Film.

Mid-infrared plasmonic resonance enables nanoscale light confinement at mid-infrared frequencies, leading to various applications ranging from compact infrared lasers to biological and chemical sensing. However, upon fabricated, plasmonic resonators normally have a fixed resonance frequency, which limits their application frequency range and hinders the dynamic tuning potential. Here, with the flexible PEDOT:PSS ((poly(ethylenedioxythiophene):poly(styrenesulfonate)) conducting polymer as the plasmonic medium, highly tunable and reversible mid-infrared plasmonic resonances are demonstrated via mechanical stretching.

Safety and Effectiveness of Electroacupuncture During Colon Endoscopic Submucosal Dissection: A Randomized Controlled Trial.

Background: Endoscopic treatment of early colon neoplasms has evolved as a valid and less traumatic alternative to surgical resection. It can usually be performed with sedation on an outpatient basis. The present study was performed to determine the safety and effectiveness of electroacupuncture (EA) versus propofol sedation during endoscopic submucosal dissection (ESD) for early colon neoplasm.

Innate immune sensing of rotavirus by intestinal epithelial cells leads to diarrhea.

Diarrhea is the predominant symptom of acute gastroenteritis resulting from enteric infections and a leading cause of death in infants and young children. However, the role of the host response in diarrhea pathogenesis is unclear. Using rotavirus and neonatal mice as a model, we found that oral inoculation of UV-inactivated replication-defective rotavirus consistently induced watery diarrhea by robust activation of cytosolic double-stranded RNA sensing pathways and type III interferon (IFN-λ) secretion.

TEDML: a new machine learning (ML) approach for predicting thyroid eye disease and identifying key biomarkers.

Thyroid eye disease (TED) features immune infiltration and metabolic dysregulation. Understanding these processes and identifying potential biomarkers are crucial for improving diagnosis and treatment. To this end, immune cell infiltration was analyzed and gene set variation analysis (GSVA) was conducted on the GSE58331 dataset to identify differences between TED and normal tissues.

Clinical application of three-dimensional printing technology in laparoscopic right hemicolectomy for colon cancer: a pilot study and video demonstration.

Background: Patients who undergo laparoscopic right hemicolectomy often have vascular anomalies, creating challenges for surgeons. Preoperative identification of vascular anomalies and intraoperative precise navigation can enhance surgical safety and reduce the difficulty of the procedure. Accordingly, this study aimed to explore and evaluate the application of three-dimensional (3D) reconstruction and printing technology in laparoscopic right hemicolectomy and its assistance in preoperative planning and intraoperative navigation.

Single-Atom Enables Reverse Hydrogen Spillover for High-Performance Protonic Ceramic Fuel Cells.

Protonic ceramic fuel cells (PCFCs) offer a promising avenue for sustainable energy conversion, however, their commercial potential is hindered by sluggish proton-involved oxygen reduction reaction (P-ORR) kinetics and inadequate durability of cathode materials. Here, a novel single-atom Ru anchor on BaCeFeO (BCF) perovskite, synthesized by a facile and scalable solid-state approach, as a potential cathode for PCFCs is reported. Theoretical and experimental analyses demonstrate that the single-atom Ru on BCF, characterized by a unique 4-coordinate Ru-O-Fe configuration, not only induces reverse hydrogen spillover but also acts as an active site for P-ORR.

Sodium taurocholate co-transporting polypeptide deficiency attenuates acetaminophen-induced hepatotoxicity via regulating expression of drug metabolism enzymes in mice.

Acetaminophen (APAP) overdose can induce liver injury and is generally accompanied by disruption of bile acid homeostasis. Physiologically, sodium taurocholate co-transporting polypeptide (NTCP) participates in the uptake of bile acids from portal blood into hepatocytes to maintain enterohepatic recirculation but its role in APAP-induced hepatotoxicity is unclear. Wild-type (WT) C57BL/6J and NTCP knockout (KO) mice were injected with 400mg/kg APAP and liver injury was evaluated by serum biochemical markers and histologic evaluation.

Hydrogen production by suspension self-rotation enhanced pyrolysis of sludge particles in cyclone.

The challenges faced by sludge pyrolysis units, including poor heat transfer efficiency and uneven heating of material groups, significantly hinder the green and low-carbon transformation and sustainable development of sludge treatment. The suspension self-rotation of sludge particles in a cyclone enhances particle heat transfer, thereby improving the pyrolysis process. In this study, we developed a novel method for sludge pyrolysis using Cyclone Suspension Self-Rotation Pyrolysis Reactor (CSSPR).