Multi-omics analysis identifies an M-MDSC-like immunosuppressive phenotype in lineage-switched AML with KMT2A rearrangement.

Lineage switching (LS) is the conversion of cancer cell lineage during the course of a disease. LS in leukemia cell lineage facilitates cancer cells escaping targeting strategy like CD19 targeted immunotherapy. However, the genetic and biological mechanisms underlying immune evasion by LS leukemia cells are not well understood.

Patterns and Environmental Drivers of Soil Microbial Succession.

Succession has been a central theme of ecology for over a century, yet the patterns and drivers of soil microbial succession remain less well understood. Here, we analyzed the raw sequencing data of 5184 soil samples involving microbial succession, including primary succession, forest and grassland secondary succession. We provide the first evidence that the β-diversity (β-total, compositional dissimilarity between communities) of soil bacterial and fungal communities both decreased significantly with successional age in the three successional types.

Predicting the detonation properties of energetic materials through phonons.

Context: Understanding the microscopic mechanisms underlying the detonation behavior of energetic materials is crucial for the development of safer and more efficient explosives. In this work, we employ first-principles calculations to optimize the molecular geometries of eight energetic compounds and analyze their phonon characteristics, including the number of molecular vibration modes in the doorway region (j) and the frequency gap (∆w). A new parameter, the phonon energy transfer rate, is defined and found to exhibit a strong linear correlation with detonation velocity (R = 0.

Constructing Stable Sub/Surface Structure to Boost Superior Cyclabilities of Single-Crystalline Ni-Rich Cathode.

Under prolonged high-voltage cycling, single-crystalline Ni-rich cathodes are prone to severe transition metal dissolution, irreversible phase transformations, and reduced structural stability, which significantly hinder their practical application. Hence, a dual-modification strategy is proposed and implemented for single-crystalline LiNiCoMnO (SCNCM90) cathodes by introducing Al/B gradient co-doping and an AlBO surface coating to mitigate anisotropic structural changes. The subsurface Al/B gradient doping induces a lithium-rich vacancy disordered structure, which effectively suppresses the H2-H3 phase transition, while suppressing lattice strain and mechanical degradation.

Modulating Interfacial Solvent Aggregation Chemistry to Enable Low-Temperature Sodium-Ion Battery.

The capability of sodium-ion batteries (SIBs) to operate under extreme temperatures is highly desirable; however, achieving stable performance remains challenging due to limitations in interfacial dynamics. Here, it is revealed that at low temperatures, linear solvents tend to aggregate within the inner Helmholtz plane (IHP), leading to the formation of a solvent-derived solid-electrolyte interphase (SEI) with sluggish Na diffusion kinetics. To address this issue, it is proposed to leverage the polarization interaction induced by the orbital overlap between the solvent molecules and free radicals as an effective approach to breaking solvent aggregation.

In Situ Engineering of Zwitterionic Betaine-Based Covalent Organic Frameworks Boost Cooperative CO Catalytic Conversion.

The development of a privileged organocatalyst featuring well-defined catalytic sites to drive highly cooperative CO conversion into high value-added fine chemicals is promising while challenging. Here in this work, three zwitterionic betaine-based covalent organic frameworks (COFs) bearing sulfonate, carboxylate, and phosphate moieties, namely, PS-TpBpy, BA-TpBpy, and PA-TpBpy, are in situ engineered through the post-synthetic quaternization of the bipyridine COF (TpBpy). Density functional theory-based Fukui function indicated that the pyridinium cation and the alkaline O anion in these betaine-based COFs serve as synergistic electrophilic and nucleophilic active sites, thus simultaneously activating o-phenylenediamine and CO for their highly efficient conversion to benzimidazole.

Hydrogen Bridge-Mediated Efficient Electrooxidation of 5-Hydroxymethylfurfural on Ni(OH)─PO /Ni(PO) Heterojunctions.

Accelerating proton deintercalation and transfer on the catalyst surface is crucial for the electrochemical oxidation of 5-hydroxymethylfurfural (HMF) into the high-value 2,5-furanodicarboxylic acid (FDCA). Herein, we have constructed a Ni(OH)─PO /Ni(PO) heterojunction catalyst that demonstrates exceptional selectivity (97.16%), yield (94.

Potent Fluorescent Probe for Target-Engagement Studies of Allosteric Pyruvate Kinase Modulators.

Pyruvate kinases (PKs) are highly allosterically regulated enzymes that play a central role in cellular metabolism and are increasingly recognized as valuable therapeutic targets in cancer, metabolic diseases, and diabetes. Despite their biological and clinical significance, methods to directly assess allosteric ligand engagement of PK isoforms remain limited. Here, we report the development of LumiPK, a novel, environment-sensitive fluorescent tracer designed to monitor allosteric binding to the liver isoform of pyruvate kinase (PKL).

Cyclometalating Ligand Affords NIR Absorption in Ruthenium Complexes for Type-I Photodynamic Therapy.

The development of NIR photosensitizers (PSs) based on Ru complexes with Type-I process holds substantial promise for photodynamic therapy (PDT) of cancer, though this breakthrough remains unrealized to date. Herein, a pair of cyclometalated Ru enantiomers, [Λ/Δ-Ru-dqpy-TPABP]Cl (dqpy: 2,6-di(quinolin-2-yl)pyridine; TPABP: 4-(4-(pyridine-2-yl)-2,1,3-benzothiadiazol-7-yl)triphenylamine) (Λ/Δ-Ru-TPABP), were synthesized and evaluated. These complexes exhibit strong Ru(d) and TPABP(π) → dqpy(π*) charge transfer ((metal and ligand)-ligand charge transfer; ML-LCT) absorption at ∼640 nm, with an extended absorption tail reaching up to 800 nm.

Control Over S(VI) Stereogenicity for the Asymmetric Synthesis of Sulfonimidoyl Derivatives by Isothiourea-Catalyzed Covalent Activation of Sulfur(VI) Atoms.

In contrast to the notable advancements focusing on the preparation of optically enriched S(IV) frameworks in recent years, achieving catalyst stereocontrol over S(VI) stereogenicity to generate chiral S(VI) scaffolds remains a largely underexplored challenge. Herein, we document a new activation mode of isothiourea organocatalysis for the highly enantioselective synthesis of S(VI)-chiral sulfonimidates. This method involves the covalent activation of racemic S(VI) sulfonimidoyl chlorides through the formation of a pivotal isothiourea-bound sulfonimidoyl intermediate.

Cerebrospinal fluid leptin in Alzheimer's disease: relationship to plasma levels and to cerebrospinal amyloid.

Objectives: Dysregulation of brain leptin signaling contributes to Alzheimer's disease (AD) pathophysiology, but plasma leptin may not accurately reflect central nervous system activity. This study examined the correlation between plasma and cerebrospinal fluid (CSF) leptin levels and their relationship with beta-amyloid (Aβ) and tau biomarkers.

Methods: Cross-sectional analysis of data from cognitively impaired patients from a tertiary memory clinic.

Conditioning intensity in myelodysplastic patients aged ≥ 50 years undergoing allogeneic hematopoietic cell transplantation (allo-HCT): a study on behalf of the chronic malignancies working party of the EBMT.

Reduced intensity conditioning (RIC) is usually used for patients with myelodysplastic syndrome (MDS) undergoing allogeneic hematopoietic cell transplantation (allo-HCT), particularly in the elderly or those with comorbidities. The impact of conditioning intensity on patients' outcome remains controversial with clinicians' subjective opinion/ experience remaining a major guide in choosing the intensity. Here, we compare RIC versus MAC in a large EBMT retrospective study in MDS patients aged ≥50 years undergoing allo-HCT between 2014 and 2018.

Single-cell atlas of human liver and blood immune cells across fatty liver disease stages reveals distinct signatures linked to liver dysfunction and fibrogenesis.

Immune cells play a central yet poorly understood role in metabolic dysfunction-associated steatotic liver disease and metabolic dysfunction-associated steatohepatitis (MASLD/MASH), a global cause of liver disease with limited treatment. Limited access to human livers and lack of studies across MASLD/MASH stages thwart identification of stage-specific immunological targets. Here we provide a unique single-cell RNA sequencing atlas of paired peripheral blood and liver fine-needle aspirates from a full-spectrum MASLD/MASH human cohort.

Whole-Brain Analysis of Increased Brain Iron in Patients with β-Thalassemia.

Rationale And Objectives: β-thalassemia (β-TM) is an inherited hematological disorder characterized by impaired hemoglobin synthesis, leading to chronic anemia and systemic iron overload. While numerous studies have documented widespread iron deposition caused by repeated blood transfusions and disrupted iron metabolism, the impact of iron overload on the brain remains poorly understood. This study aimed to investigate whole-brain iron accumulation in patients with β-TM using quantitative susceptibility mapping (QSM).

From senescence to scarring: Exploring TGF-β signaling in cellular aging, fibrotic remodeling, and pulmonary fibrosis.

Cellular senescence and the formation of fibrotic scarring are critical in the progression of chronic illnesses, such as pulmonary fibrosis (PF). In this context, the transforming growth factor-beta (TGF-β) pathway represents a central driver in orchestrating the pathological cascade. TGF-β governs cellular activities such as differentiation, apoptosis, and extracellular matrix (ECM) remodeling as a pleiotropic cytokine.

Using a Vortex Whistle System to Estimate Phonatory Airflow via the Phonation Quotient.

Introduction: The primary purpose of this study was to examine the relationship between phonation quotient (PQ) obtained using a low-cost vortex whistle system (VWS) vs phonatory airflow (PA) in sustained voicing calculated using a clinical-standard pneumotach-based system (the Phonatory Aerodynamic System [PAS]). PQ values obtained using the VWS were also compared with PQ values obtained using a "gold" standard pneumotach-based Koko Sx1000 spirometer.

Methodology: Participants were 91 vocally healthy young adults (61 females and 30 males) between the ages of 18 and 30 years.

High-Resolution aCGH Analysis of the Jurkat Cell Line: Copy Number Alterations and Their Association With Cancer Hallmarks.

Background/aim: Cell lines serve as valuable models to study altered cellular signaling pathways, to identify mutations in key oncogenic genes, and to test potential antitumor drugs. The Jurkat cell line, for example, has provided important information about various signaling pathways in lymphoblastic leukemia, establishing most of what is currently known about T-cell receptor (TCR) signaling. However, many aspects of the genome modification of this cell line have not yet been analyzed.

PPIL1 Drives Hepatocellular Carcinoma Progression and Cancer Stem Cell Self-renewal Through DAAM2-mediated Wnt/β-Catenin Activation.

Background/aim: Hepatocellular carcinoma (HCC) accounts for ~90% of primary liver cancer, which ranks as the third-leading cause of global cancer mortality. Emerging evidence establishes cancer stem cells (CSCs) as central regulators of HCC progression, metastasis, and therapeutic resistance, with stemness-related pathways like Wnt/β-catenin signaling critically maintaining CSC self-renewal. In this study, we aimed to investigate the role of Peptidyl-prolyl isomerase-like 1 (PPIL1) in HCC progression and CSC self-renewal.

Anxiety, depression and family resilience: A three-level meta-analysis.

Background: Perceived family resilience, which refers to the subjective assessment of family members regarding their family's ability to effectively adapt and maintain stability during challenging times, has been negatively correlated with mental health issues such as anxiety and depression in numerous studies; however, the strength of this association has varied significantly across previous research. This study fills this gap through a three-level meta-analysis.

Method: This study aims to obtain reliable estimates of effect sizes and explore potential moderating factors in the relationship between perceived family resilience and symptoms of anxiety and depression.

Selenized neural stem cell-derived exosomes: A neotype therapeutic agent for traumatic injuries of the central nervous system.

Oxidative damage and neuroinflammation are the key features of central nervous system (CNS) injury. Inspired by the neuroprotective properties of neural stem cell-derived exosomes (NExo) and the reactive oxygen species (ROS) scavenging ability of selenium, we develop an advanced NExo bearing ultrasmall nano-selenium (∼3.5 nm) via lipid-mediated nucleation (SeNExo).

Zirconium‑tin oxide interlayer modification enhances the performance and stability of the titanium-based lead dioxide electrode for efficient electrocatalytic degradation of alizarin yellow R.

In this study, a novel titanium/zirconium‑tin oxide/alpha‑lead dioxide/beta‑lead dioxide (Ti/Zr-SnO/α-PbO/β-PbO) electrode was prepared and utilized for degrading alizarin yellow R (AYR) dye. The Integration of impregnation drying, drop coating with a solvent, and high-temperature pyrolysis significantly diminished the characteristic fissure morphology of conventional pyrolytic metal oxide interlayers, thereby modulating the electrodeposition process of alpha‑lead dioxide (α-PbO) and enhancing electrode stability and electrochemical performance. Electrode characterization revealed that the charge transfer resistance (R) was 88.

Autoimmune diabetes and targets for immunomodulation.

In Type 1 diabetes (T1D), pancreatic β cells are progressively lost, resulting in insulin insufficiency. Although exogenous insulin is essential for disease management, it is not a cure, and inadequate glycemic control continues to result in long-term complications. Thus, there is a need for therapies that address the underlying autoimmune response, a key component of T1D pathogenesis.

Efficient Synthesis of Protein-Polymer Conjugates with Open-Air Fabrication and Facile Purification Driven by the Thermoresponsive Protein-Polymer Conjugate.

A simple, efficient, and broadly applicable method for the preparation of protein-polymer conjugates is of critical importance for their further development. However, existing approaches are often limited by challenges such as insufficient deoxygenation, complex purification processes, and poor compatibility of monomers and proteins. Herein, we present a comprehensive strategy for the synthesis of protein-polymer conjugates utilizing a thermosensitive, recyclable glucose oxidase-poly-(di-(ethylene glycol) methyl ether methacrylate) conjugate (GOX-PDEGMA) as part of the deoxygenation system.

The Borderline Symptom List-Interview: development and psychometric evaluation of an observer-based instrument for assessing symptom severity in borderline personality disorder.

Background: Borderline Personality Disorder (BPD) is a complex mental health condition characterized by pervasive instability in mood, interpersonal relationships, self-concepts, and behavior. A reliable assessment of BPD symptom severity is essential for effective treatment planning and evaluation. This study introduces and evaluates the Borderline Symptom List Interview (BSL-I), a semi-structured interview designed to assess the severity of BPD symptoms comprehensively.