Seasonal habitat suitability analysis of in the northern Tanggula region of Sanjiang-yuan National Park, China.

is endemic to the Qinghai-Tibet Plateau that possesses both keystone and flagship qualities. The Tangbei Area (north of the Tanggula Mountains) in the Sanjiangyuan National Park is one of the core distribution areas for . Both population size and extinction risk of are concerning for conservation and action is needed.

PPIL1 Drives Hepatocellular Carcinoma Progression and Cancer Stem Cell Self-renewal Through DAAM2-mediated Wnt/β-Catenin Activation.

Background/aim: Hepatocellular carcinoma (HCC) accounts for ~90% of primary liver cancer, which ranks as the third-leading cause of global cancer mortality. Emerging evidence establishes cancer stem cells (CSCs) as central regulators of HCC progression, metastasis, and therapeutic resistance, with stemness-related pathways like Wnt/β-catenin signaling critically maintaining CSC self-renewal. In this study, we aimed to investigate the role of Peptidyl-prolyl isomerase-like 1 (PPIL1) in HCC progression and CSC self-renewal.

Mesoporous vanadium-doped FeO nanozymes with enhanced peroxidase-mimetic activity for colorimetric/electrochemical dual-mode detection of nitrite in food and seawater samples.

Developing iron-based nanozymes with highly active sites and elucidating their catalytic mechanisms hold great promise for advancing applications in food safety, environmental monitoring and clinical medicine. Herein, vanadium (V)-doped FeO (V-FeO) nanozymes with peroxidase (POD)-mimetic activity were fabricated using a post-doping method and employed to construct a colorimetric/electrochemical dual-mode sensor for quantitative analysis of nitrite (NO). Experimental and density functional theory data show that the dopant of V into the structure of V-FeO nanozymes can enhance their POD-mimetic activity due to the increased oxygen vacancies and the improved adsorption ability for HO.

Roadmap for Next-Generation Electrochemical Energy Storage Technologies: Secondary Batteries and Supercapacitors.

The transition from fossil fuels to environmentally friendly renewable energy sources is crucial for achieving global initiatives such as the carbon peak and carbon neutrality. The use of secondary batteries and supercapacitors based on electrochemical energy storage principles provides high energy density, conversion efficiency, and rapid response times, offering essential solutions for stabilizing and ensuring the reliability of energy supply from renewable sources despite their intermittency. In recent years, increased demands for higher energy density, improved rate performance, longer cycle life, enhanced safety, and cost-effectiveness have driven researchers to delve deeper into electrode materials, electrolytes, and storage mechanisms in secondary batteries.

Enhancer Placement Impacts Transcriptional Dynamics in Embryos.

The functional role of enhancer-promoter configurations in transcription regulation remains poorly understood, despite the wide range of linear genomic distance and relative enhancer positioning observed in endogenous contexts. While canonical models suggest that enhancers operate independently of genomic position, technical limitations have obscured insights on transcriptional kinetics. Here, we employ single-cell MS2/MCP-based live imaging in embryos to systematically analyze transcriptional dynamics driven by sub-10kb enhancer-promoter arrangements.

Prognostic Value of Clinical Complete Response for Patients with Initially Unresectable Hepatocellular Carcinoma after Conversion with Triple Therapy.

Introduction: Accurately predicting the outcomes of conversion therapy among patients with initially unresectable hepatocellular carcinoma (uHCC) remains a challenge. Clinical complete response (cCR) has been proposed as a predictor of prognosis. However, information on its prognostic value in these patients is limited.

Targeting Reticulin 4 (RTN4) Within Small Extracellular Vesicles Combats Metastasis and Reinforces Immunotherapy in Triple-Negative Breast Cancer.

Small extracellular vesicles (sEV) are a class of natural vesicles rich in heterogeneous cargos with the great advantage of non-invasive detection; these vesicles exhibit complex intercellular crosstalk and mediate important biological functions. However, the potential value of plasma sEV in clinical prognosis prediction of triple-negative breast cancer (TNBC) and their biological functions have not been well elucidated. In this study, we isolated sEV from non-metastatic and metastatic TNBC plasma samples.

Single-Step Latent Diffusion for Underwater Image Restoration.

Underwater image restoration algorithms seek to restore the color, contrast, and appearance of a scene that is imaged underwater. They are a critical tool in applications ranging from marine ecology and aquaculture to underwater construction and archaeology. While existing pixel-domain diffusion-based image restoration approaches are effective at restoring simple scenes with limited depth variation, they are computationally intensive and often generate unrealistic artifacts when applied to scenes with complex geometry and significant depth variation.

Protocol for vibratome sectioning, immunofluorescence, and S-phase labeling of inner ear organoids.

Inner ear organoids represent a potentially inexhaustible source of otic tissues, including sensory hair cells and supporting cells, for in vitro manipulation. Here, we present a protocol for labeling S-phase entry of cells in inner ear organoids using 5-ethynyl-2'-deoxyuridine (EdU), followed by fixation and vibratome sectioning. Nuclear EdU is then detected alongside protein markers of interest via immunofluorescence.

α-Synuclein Promotes the Expression and Release of Neurofilament Light Chain from Oligodendrocytes in a Murine Model: Implication for Multiple System Atrophy?

Objective: The understanding of neurofilament light chain (NfL) presence and release in oligodendrocytes is limited. This study aims to determine the NfL expression in oligodendrocytes, investigate whether excess ɑ-synuclein (α-syn) impacts NfL expression, release, and functions in oligodendrocytes, as well as detect plasma NfL-positive oligodendrocyte-derived extracellular vesicles (ODEVs) changes in patients with multiple system atrophy (MSA), Parkinson's disease (PD), and healthy controls (HCs).

Methods: NfL expression in oligodendrocytes was assessed using RNAscope and immunofluorescence staining in human and mouse brain slices, as well as flow cytometry in mouse brain single-cells.

Assessment of Choriocapillaris in Healthy and Diseased Eyes Using 3-Dimensional Topographic Maps Based on OCT Angiography.

Purpose: To achieve clear in vivo imaging and systematic quantification of choriocapillaris (CC), enabling the exploration of age-related and disease-related changes of CC within a large sample size.

Design: Cross-sectional study.

Participants: A total of 1050 eyes from 727 individuals, comprising 633 healthy eyes, 277 eyes with age-related macular degeneration (AMD), and 140 eyes with high myopia.

Three new cyclopeptides from the Lycii cortex.

Ten cyclopeptides were isolated from the 70 % ethanol extract of Lycii Cortex and identified by physical and chemical properties and various spectroscopic methods. They were named as lyciumin E (1), lyciumin G (2), lyciumin F (3), lyciumin B methyl ester (4), lyciumin A methyl ester (5), lyciumin C methyl ester (6), lyciumin A (7), lyciumin B (8), lyciumin C (9) and lyciumin D (10). Among them, compounds 1-3 were new cyclopeptides, compound 4 was a new natural product, and compounds 5 and 6 were first isolated from this plant.

Longitudinal Analysis of the Immunostimulatory Properties and Safety Profile of LRa05 as a Dietary Supplement.

Supplementation with appropriate doses of has been reported to potentially attenuate immune changes. While some progress has been made in studying the immune function of LRa05, a lack of systematic summaries has led to the neglect of its application in enhancing immune function. This study aimed to investigate the effects of LRa05 on the immune function of mice and to evaluate its safety profile as a dietary supplement.

High intensity forced ultrasound-driven ferroptosis as a strategy for anti-tumor immune priming.

Cold tumors have a poor response to tumor immunotherapy due to low immune cell infiltration and the ability to evade immune attacks. Converting cold tumors into hot tumors can enhance the clinical effectiveness of anti-tumor immunotherapy. High-intensity focused ultrasound (HIFU) as a non-invasive treatment can damage tumors through mechanical effects, but there is a lack of research on its cytotoxic mechanisms at the cellular level and its role in inducing anti-immune responses.

Colorectal Polyp Size Measurement Faces Infinite Possibilities: Artificial Intelligence Is the Key.

Background: Colorectal neoplasia poses a severe health threat worldwide. The accurate measurement of polyp size is essential for risk stratification, selecting polypectomy techniques, and determining the surveillance interval.

Summary: The methods routinely used for measuring polyp size, including objective ex vivo measurement, subjective visual estimation by an endoscopist, and objective precise measurement using endoscopic instruments, all have limitations.

Long-term exposure to PM and multidimensional poverty in middle aged and older people in China: a quasi-experimental study.

Background: Environmental pollution not only poses a direct threat to human health but also exacerbates economic inequality through various channels. The China Clean Air Action (CCAA) has been shown to alleviate the health burden caused by air pollution. Although the link between environmental degradation and poverty have been increasingly recognized, there are few studies that systematically assess the impact of the CCAA on reducing multidimensional poverty and the role of respiratory diseases (RD) in this context.

An omics-based drug-HIFU combination therapy discovery for ferroptosis treatment of TNBC.

Combination therapy, as a vital strategy in cancer treatment, aims to overcome the limitations of monotherapies by combining two or more drugs or treatments to enhance antitumor efficacy. However, the unclear interactions between different therapies and the difficulty of precisely identifying effective combination treatments remain major challenges. In this study, we developed an omics-based drug-HIFU combination therapy discovery framework taking ferroptosis treatment of triple-negative breast cancer (TNBC) as a paradigm.

The Impact of Different Factors on Postoperative Appearance and Quality of Life in Patients With Ear Injury: A Retrospective Study.

Background: The ear is a common site of facial injury, affecting facial aesthetics and patients' psychological well-being. Postoperative outcomes vary significantly, and this study aims to explore the influencing factors through retrospective analysis, providing references for clinical treatment.

Methods: A total of 103 patients were included in this study.

Resveratrol: Harnessing Nature's Potential for Chronic Pain Relief.

Resveratrol, a natural polyphenol with anti-inflammatory, antioxidant, and neuroprotective properties, shows great potential in managing chronic pain. This review explores its analgesic mechanisms, including the inhibition of neuroinflammation, enhancement of antioxidant activity, induction of autophagy, reduction of endoplasmic reticulum stress, modulation of the serotonin system, restoration of gut microbiota homeostasis, regulation of the neuroendocrine system, and promotion of mitochondrial biogenesis. While its analgesic potential is considerable, future research should prioritize enhancing its bioavailability, investigating drug interactions, and confirming long-term safety to develop more effective therapies for chronic pain.

Long-Term Outcome of Repeat Resection versus Radiofrequency Ablation for Intrahepatic Recurrence after Curative Resection of Hepatocellular Carcinoma Initially within Barcelona Clinic Liver Cancer Staging System 0-A Stages: A Multicenter Study.

Introduction: Hepatocellular carcinoma (HCC) is the most common primary liver cancer with an extremely high recurrence rate. Due to the absence of definitive treatment guidelines for recurrent HCC, this study assessed the effectiveness of repeat resection (RR) versus percutaneous radiofrequency ablation (RFA) in patients initially diagnosed with Barcelona clinic liver cancer staging system (BCLC) stage 0-A primary HCC who developed no more than three recurrent tumors, each ≤3 cm in size, after curative resection.

Methods: This study retrospectively analyzed patients diagnosed with recurrent HCC between January 1, 2010, and May 30, 2022, across five centers, who underwent either RR or RFA.

Long-term survival in advanced unresectable HCC treated with transcatheter arterial chemoembolization combined with lenvatinib and PD-1 inhibitors.

Background: Transcatheter arterial chemoembolization combined with lenvatinib and PD-1 inhibitors (triple therapy) is a promising therapy for unresectable hepatocellular carcinoma (uHCC). We aimed to assess the characteristics and identify predictors of long-term survival (LTS) in advanced uHCC treated with triple therapy.

Methods: Retrospectively reviewed patients with uHCC who underwent triple therapy between June 2018 and May 2023 at 8 hospitals in China.

Inhibition of CYP1B1 by miR-200b-3p increases the sensitivity of paclitaxel in hepatocellular carcinoma treatment.

Liver cancer ranks as the third leading cause of cancer-related death worldwide, with hepatocellular carcinoma (HCC) accounting for approximately 90 % of primary liver cancer cases. Elevated expression of drug-metabolizing enzyme CYP1B1 in HCC has been identified as a potential contributor to primary paclitaxel (PTX) resistance. This study demonstrated that miR-200b-3p suppresses CYP1B1 expression in HCC cells.

Crotonylation driving Streptococcus pneumoniae adaption and virulence.

Introduction: To cope with the host's stressful environment and immune clearance, some drug-resistant bacteria have reduced their virulence. Protein post-translational modifications (PTMs) are known to regulate numerous critical biological processes; however, their specific role in modulating bacterial virulence remains poorly understood.

Objective: This study seeks to elucidate the mechanistic role of lysine crotonylation (Kcr) in modulating Streptococcus pneumoniae virulence and adaptive survival.

Biological functions and detection strategies of magnesium ions: from basic necessity to precise analysis.

Magnesium is a ubiquitous element in the natural environment and plays an indispensable role in various biological processes in living organisms. This review focuses on the diverse functions of magnesium ions (Mg) in living cells, particularly emphasizing their role in the immune and cardiovascular systems. We discuss how Mg regulates key cellular processes, including enzyme catalysis, cellular signaling and nucleic acid structural stabilization, and explore the adverse effects of magnesium imbalance, which has been associated with numerous diseases ranging from cardiovascular to neurological disorders.