Transcriptomic and Lipidomic Analyses Reveal a Novel Antiapoptotic Mechanism of Peptide IRW in the Arteries of Spontaneously Hypertensive Rats through the Reduction of Ceramide Levels and Arachidonic Acid Release.

IRW, an antihypertensive peptide derived from ovotransferrin, has been shown to lower blood pressure in spontaneously hypertensive rats (SHRs) by upregulating angiotensin-converting enzyme 2 (ACE2). ACE2 is cardioprotective and a well-documented inhibitor of apoptosis. This study aims to investigate the anti-apoptotic effects of IRW and its underlying mechanism in SHRs' vasculature.

Pressure effects on lithium anode/nickel-manganese-cobalt oxide cathode pouch cells through fixture design.

Applying external pressure to a pouch cell results in improved performance, implicating systems-level design of batteries. Here, different formats and amounts of external pressure to Li-LiNiMnCoO (Li-NMC811) pouch cells were studied under lean electrolyte conditions. Due to the more uniform lithium plating/stripping, a constant gap fixture that retains the distance of the frame during cycling performed greater than a constant pressure fixture that retains applied pressure to the cell.

Self-healing Cu single-atom catalyst for high-performance electrocatalytic CO methanation.

To address the escalating challenge of atmospheric CO emissions, this study proposes a self-healing Cu single atom (SA) catalyst design. By partially cleaving Cu-N bonds via hydrogen evolution reaction (HER), coordinatively unsaturated Cu sites form and spontaneously bond with adjacent ZrO clusters which are strategically positioned near the Cu SA, creating a hybrid Cu-N/O structure with enhanced performance. In situ Raman and X-ray absorption fine structure (XAFS) measurements confirm the dynamic reconstruction of coordination environment from CuN to CuNO under electrochemical conditions.

Mitophagy as a pivotal axis in non‑alcoholic fatty liver disease: From pathogenic mechanisms to therapeutic strategies (Review).

Non‑alcoholic fatty liver disease (NAFLD), characterized by excessive lipid accumulation in hepatocytes, has emerged as the leading cause of chronic liver disorders globally. As the central metabolic organ, the liver critically depends on mitochondrial integrity. Mitophagy, a selective form of autophagy, plays a pivotal role in sustaining mitochondrial homeostasis by eliminating dysfunctional mitochondria.

Nurse prescribing right: a bibliometric analysis.

Background: The concept of nurse prescribing rights is undergoing rapid evolution, and there is increasing interest in academic circles regarding research on this topic.

Objective: This study aims to conduct a comprehensive bibliometric analysis of nurses' prescribing rights to understand the research background, emerging trends, and relevant academic impacts in this field, so as to provide valuable insights for the formulation of health policies and the promotion of more equitable and efficient healthcare services.

Methods: We conducted a bibliometric analysis of the articles on nurses' prescribing rights from the establishment of the database until 6 December 2023.

Meta-analysis on the safety and efficacy of Erdafitinib in treating FGFR1-4 mutated solid tumors.

Objective: This article aims to analyze the safety and efficacy of Erdafitinib in the treatment of patients with advanced solid tumors harboring FGFR1-4 mutations.

Methods: Search for relevant articles in databases such as PubMed, Embase, The Cochrane Library, Web of Science, and CNKI, covering the period from their establishment to October 25, 2024. Summarize the adverse drug reaction (AE) data, overall survival (OS), median progression-free survival (PFS), objective response rate (ORR), and other relevant data for patients with advanced solid tumors treated with Erdafitinib for FGFR1-4 mutations.

Efficacy and Safety of the Bushen Quhan Zhiwang Decoction to Achieve Clinical Deep Remission in Rheumatoid Arthritis: Protocol for a Double-Blind, Randomized, Placebo-Controlled Trial.

Purpose: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by significant disability, with effective management of disease activity and pain constituting critical clinical objectives. The herbal formulation Bushen Quhan Zhiwang Decoction (BQZD) has demonstrated potential efficacy in symptom alleviation, suggesting a promising role in achieving sustained clinical remission in RA. The present study aims to systematically evaluate the efficacy and safety profile of BQZD for managing RA disease activity and pain relief.

Fibroblast activation protein-α drives rheumatoid arthritis inflammation through the AKT/mTOR signaling pathway and its therapeutic effect by Wangbi granules.

Background: Fibroblast activation protein-α (FAPα), a transmembrane protein highly expressed in rheumatoid arthritis (RA) synovium, is postulated to drive inflammatory cascades, yet its mechanistic role remains elusive. Wangbi granules (WBG), a clinically used traditional Chinese medicine (TCM), show anti-RA potential but lack defined pharmacological evidence.

Purpose: To identify FAPα as an inflammatory driver in RA pathogenesis and decipher how WBG suppresses FAPα-mediated AKT/mTOR signaling to resolve inflammation.

Arabidopsis MACP2 contributes to autophagy induction by modulating starvation-induced reactive oxygen species homeostasis.

In plants, autophagy is a conserved recycling system essential for development and stress responses by targeting cellular components for massive degradation in the vacuole. Our previous work suggested that autophagy contributes to Arabidopsis (Arabidopsis thaliana) stress responses by modulating NADPH-oxidase-mediated reactive oxygen species (ROS) homeostasis; however, the molecular link between extracellular ROS and autophagy remains unknown. We performed a yeast two-hybrid screen to identify components involved in autophagy, using the central autophagy component ATG8e as a bait.

Integrated spatial transcriptome and metabolism study reveals metabolic heterogeneity in human bladder cancer.

Bladder cancer (BC) is a malignancy that originates from the cells lining the bladder and is one of the most common cancers of the urinary system, capable of occurring in any part of the bladder. However, the molecular mechanisms underlying the malignant transformation of BC have not been systematically studied. This study integrated cutting-edge techniques of spatial transcriptomics (ST) and spatial metabolomics (SM) to capture the transcriptomic and metabolomic landscapes of both BC and adjacent normal tissues.

Research on Fatigue Life Prediction Method of Spot-Welded Joints Based on Machine Learning.

Spot-welding joints are widely used in modern industries, and their fatigue life is crucial for the safety and reliability of structures. This paper proposes a method for predicting the fatigue life of spot-welding joints by integrating traditional structural stress methods and machine learning algorithms. Systematic fatigue tests were conducted on Q&P980 steel spot-welding joints to investigate the influence of the galvanized layer on fatigue life.

Tumour extracellular vesicle surface Protein-mRNA integration assay for early detection of epithelial ovarian cancer.

Background: Early detection of epithelial ovarian cancer (EOC) is crucial for improving clinical outcomes. However, the sensitivity of primary serological marker cancer antigen 125 (CA125) is suboptimal for detecting early-stage EOC. Tumour-derived extracellular vesicles (EVs) are promising biomarkers for early cancer detection.

Integrated 'Shield-Spear' Biological Patch for Fibrosis-Free Bladder Reconstruction.

Bladder reconstruction without fibrosis remains a global challenge. Current bladder defect treatments primarily focus on repair at the level of vascularization, failing to balance healing and excessive collagen deposition, and neglecting the exacerbation of fibrosis due to neural dysregulation. In this study, an integrated 'shield-spear' patch composed of an anionic 'shield' hydrogel (HAD) and neuro-targeted 'spear' engineered extracellular vesicles (S100Aptamer-EVs) using Schiff base chemistry and Michael addition reactions is engineered.

Low-crosstalk, filter-free, and secure WDM for optical inter-CubeSat communication using fluorescent fiber antennas.

In this paper, a low-crosstalk, filter-free, and secure wavelength-division multiplexing (WDM) scheme based on fluorescent fiber antennas is proposed and demonstrated for optical inter-CubeSat communication (OICSC) systems. The integration of fluorescent fiber antennas in OICSC systems facilitates efficient WDM transmission to enhance the achievable data rate, owing to their inherent optical filtering capabilities. By selecting light-emitting diodes (LEDs) with distinctive emission wavelengths and fluorescent fiber antennas with the corresponding absorption wavelengths, physical WDM signal separation can be achieved to effectively minimize crosstalk at the receiver side.

Prioritizing pathway signature using deep learning approach: a novel strategy for traditional Chinese medicine formula generation and optimization.

The advancement of traditional Chinese medicine (TCM) faces challenges, due to the absence of a deep understanding of TCM mechanism at the perspective of modern biomedical practices. This results in how TCM selects herbs to treat diseases or symptoms prevailingly rely on clinicals' experience or TCM ancient books, at least in part lacking scientific basis. Herein, we present a novel deep learning-based approach, named Negative-Correlation-based TCM Architecture for Reversal (NeCTAR), to optimize the generation and combination of TCM formulas for guiding empiric therapy, by which we could, to some degree, narrow the gap between TCM and modern biomedical science.

Case Report: Sclerosing angiomatoid nodular transformation of the spleen: surgery or conservative treatment?

Sclerosing angiomatoid nodular transformation (SANT)is a rare vascular sclerosing mass-like lesion, often discovered incidentally during routine imaging evaluation or during visits for the patient's primary disease. SANT has complex pathological manifestations, unknown natural course of development and rarity, which makes it difficult for clinicians and pathologists to define its true nature. We report a 55-year-old male patient who came to see a doctor due to a physical examination accidentally found a spleen mass.

Dectin-1 Drives Diabetic Retinopathy via Inducing Microglia-Mediated Inflammation and Blood-Retinal Barrier Breakdown.

Diabetic retinopathy (DR), a prevailing manifestation among diabetic patients, occurs as a major sight-threatening disorder. Dectin-1, as an innate immune receptor, has been notified as a critical modulator of diabetes mellitus. In this context, the implication of Dectin-1 in the process of DR that is still a conundrum will be addressed here.

Effect of Graphene Oxide-Based Nanodielectric on Electrical Discharge Machining Performance of Ti-6Al-4 V.

Graphene oxide (GO), a two-dimensional nanomaterial, exhibits exceptional electrical and thermal conductivity as well as outstanding tensile strength. In this study, GO nanosheets were dispersed into the dielectric fluid during electrical discharge machining (EDM) of Ti-6Al-4V alloy using copper electrodes, enabling a comprehensive evaluation of the modified dielectric's behavior and the alloy's impact on machining performance. The incorporation of GO significantly influenced both the material removal rate (MRR) and the electrode wear ratio (EWR).

Spatiotemporal R-loop accumulation orchestrates microenvironmental remodeling after spinal cord injury.

Spinal cord injury (SCI) induces severe neurological dysfunction through direct mass cell damage and secondary inflammatory molecular cascades. These cascades-initiated by damage-recruit immune cells and amplify cytokine release, exacerbating neuronal death and tissue destruction. We initially report that R-loop accumulation (three-stranded RNA-DNA hybrids with displaced ssDNA) in neural injury contexts drives neurodegeneration via neuroinflammation.

Pharmacokinetic and pharmacodynamic integration and resistance analysis of cefquinome against dynamic model.

Introduction: is a major pathogen that causes acute clinical mastitis and its recurrent episodes in dairy cows.

Methods: In this study, a peristaltic pump one-compartment open model was established to investigate the relationship between the pharmacokinetic and pharmacodynamic (PK/PD) indices of cefquinome (CFQ) against . Bactericidal effects of single high-dosage versus multiple low-dosage administrations within the same drug dosage and best-fit dosage were assessed.

Molecular-Level Understanding of Membrane Disruption Behaviors of Antimicrobial Peptides by Gradient Boosting Algorithm-Assisted Raman Spectroscopy.

Antimicrobial peptides (AMPs) can rapidly kill bacteria via disrupting the integrity of the cytoplasmic membrane. Although the understanding of the interaction between AMPs and phospholipid membrane is essential for related drug designs, the observation of this process is still challenging, especially at the molecular level. Herein, we develop a new analytical methodology named gradient boosting algorithm-assisted Raman spectroscopy (GB-Raman) for revealing the membrane disruption mechanism of AMPs.

Anti-PLA2R IgG4-to-IgG Ratio Helps to Predict Remission of PLA2R-Associated Primary Membranous Nephropathy.

Background And Aims: PLA2R-associated primary membranous nephropathy (PMN) was classified as IgG4-associated autoimmune disease in which anti-PLA2R antibody is predominantly IgG4 subclass. Our objective was to explore the capability of anti-PLA2R IgG4-to-IgG ratio for predicting remission in PLA2R-associated PMN patients.

Methods: 143 patients with anti-PLA2R IgG ≥14 RU/ml were biopsy-confirmed as PLA2R-associated PMN.

The analysis of the progressive local failure process of the Longquan Reservoir dam based on the global-local dynamic strength reduction method.

During the progressive failure process of the slope (dam), the strength parameters of the soil in the slope (dam) continuously degrade, with the degree and rate of degradation of the soil near the shear zone significantly exceeding those in other areas. To address this mechanism, this paper proposes a global-local dynamic strength reduction method that simultaneously accounts for both the physical degradation pattern of the soil and the strain softening characteristics of the shear zone. Taking the Longquan Reservoir Dam as an engineering case, a two-dimensional profile calculation model at station 0 + 142 was established using ANSYS.

Inhibiting SSBP1 enhances ferroptosis and improves the effectiveness of sorafenib treatment for liver cancer.

Liver cancer is the third leading cause of cancer‑related mortality globally, with increasing morbidity and mortality rates. Sorafenib, a multi‑kinase inhibitor, is an effective first‑line therapy for late‑stage liver cancer. However, its effectiveness is hindered by low responsiveness, high drug resistance and significant side effects.

Albumin Encapsulation of Cyanine Dye for High-Performance NIR-II Imaging-Guided Photodynamic Therapy.

Albumin encapsulation is a powerful strategy for drug delivery, yet its potential has not been fully explored for photodynamic therapy (PDT) agents. Cl-containing near-infrared (NIR) cyanine dyes are intrinsically PDT agents and tend to covalently bind with albumin; however, their PDT efficiency in tumors is largely compromised due to limited accumulation of the complex (size less than 10 nm) to the tumor site. To maximize their PDT effect while retaining sufficient NIR brightness for imaging-guided PDT, we developed a DTT-promoted encapsulation strategy to enhance singlet oxygen release for Cl-containing dyes.