[Effect of electroacupuncture on expression in blood-brain barrier in rats with cerebral ischemia-reperfusion injury by regulating HIF-1α/VEGF/MMP-9 signaling pathway].

Objectives: To observe the effect of electroacupuncture (EA) on the expression of hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor (VEGF), matrix metalloproteinases 9 (MMP-9) and tight junction proteins in rats with cerebral ischemia-reperfusion injury (CI/RI) , so as to explore its potential mechanism in alleviating injury of neurological function and blood-brain barrier (BBB).

Methods: A total of 84 male SD rats were used in the present study. The CIRI model was established by occlusion of the middle cerebral artery and reperfusion (MCAO/R), followed by dividing the CIRI rats into model group, EA group, and edaravone group (=21 in each group).

The novelty of profilin 2 in regulating pyruvate kinase M2 nuclear translocation and promoting tumor angiogenesis in lung adenocarcinoma.

Background: Profilin 2 (PFN2), indispensable in all organisms, is important for cancer initiation and progression. Here, we found PFN2 highly overexpressed in tumor tissues with poor prognosis of Lung adenocarcinoma (LUAD) patients had a novel role in remodulating angiogenesis. However, the mechanism of PFN2-mediated LUAD angiogenesis remains unelucided.

Association of serum insulin-like growth factor-1 and adrenocorticotropic hormone therapeutic response in patients with infantile epileptic spasms syndrome.

Background: Infantile epileptic spasm syndrome (IESS), a rare age-specific epileptic encephalopathy, exhibits limited therapeutic efficacy, with approximately 50% of patients showing resistance to adrenocorticotropic hormone (ACTH) monotherapy. Herein, we investigated the association between serum insulin-like growth factor-1 (IGF-1), insulin-like growth factor-binding protein-3 (IGFBP-3), their ratio, and short-term ACTH therapeutic response in IESS, alongside their correlation with video-electroencephalogram (VEEG) characteristics.

Methods: This retrospective study included IESS patients who received ACTH treatment at Shanghai Children's Medical Center from July 2021 to November 2024.

Comparative Analysis of Growth Dynamics and Relative Abundances of Gut Microbiota Influenced by Ketogenic Diet.

Unlabelled: Although the compositional alterations of gut bacteria in ketogenic diet (KD) have been intensively investigated, the causal relationship between this extreme diet and the microbiota changes is not fully understood. Here, we studied the growth dynamics of intestinal bacteria in KD. We used the CoPTR method to calculate the peak-to-trough ratio (PTR) based on metagenomic sequencing data, serving as an indicator of bacterial growth rates.

Novel variant of COL12A1 gene causing neonatal hypotonia and respiratory failure.

The COL12A1 gene encodes Collagen XII α 1 chain, forming a biologically functional Collagen XII through a trimeric structure. Collagen XII dysfunction can lead to hereditary neuromuscular diseases with phenotypic profiles ranging from rare Ullrich congenital muscular dystrophy 2 (biallelic variant) to Bethlem myopathy 2 (uniallelic variant). Currently, only 29 COL12A1 deficiency cases have been reported, mainly featuring hypotonia, progressive muscular dystrophy, and skeletal deformities.

Diagnosis and treatment recommendations for glucose transporter 1 deficiency syndrome.

Background: Glucose transporter 1 deficiency syndrome (Glut1DS) was initially reported by De Vivo and colleagues in 1991. This disease arises from mutations in the SLC2A1 and presents with a broad clinical spectrum. It is a treatable neuro-metabolic condition, where prompt diagnosis and initiation of ketogenic dietary therapy can markedly enhance the prognosis.

A Learnable and Explainable Wavelet Neural Network for EEG Artifacts Detection and Classification.

Electroencephalography (EEG) artifacts are very common in clinical diagnosis and can heavily impact diagnosis. Manual screening of artifact events is labor-intensive with little benefit. Therefore, exploring algorithms for automatic detection and classification of EEG artifacts can significantly assist clinical diagnosis.

Comparative analysis of two-hour creatinine clearance and the C-G formula for renal function assessment in critically ill patients.

Objective And Rationale: To investigate if the 2-h creatinine clearance (Ccr) provides a more precise and timely assessment of renal function in critically ill patients compared to the Cockcroft-Gault formula (Cr).

Materials And Methods: This cohort study incorporated 74 patients who were hospitalized for more than 48 h in the Intensive Care Unit over 6 months. A 24-h urine collection protocol was observed, and concurrently, 316 2-h urine specimens were obtained.

A rare case report: Myopathy related to the interaction between azathioprine and infliximab in the treatment of ulcerative colitis and ankylosing spondylitis.

Key Clinical Message: Administering azathioprine or infliximab for UC and AS treatment carries a significant risk of adverse reactions. Here, we present the case diagnosed with UC and AS, who received treatment with azathioprine and infliximab for 10 months, and subsequently developed drug-induced myopathy affecting the right vastus medialis muscle.

Abstract: Drug-induced myopathy is an uncommon form of muscle injury that can arise in patients without preexisting muscle conditions when exposed to therapeutic doses of certain medications.

Effects of adjunctive Chinese patent medicine on outcome of viral encephalitis in children: A multicenter retrospective study in China.

Background: Some patients with viral encephalitis in China seek treatment with Chinese patent medicine (CPM) to improve their symptoms, but few studies have focused on the impact of CPM on the prognosis of viral encephalitis (VE). The aim of this multicenter retrospective study was to assess the benefit of adjunctive CPM therapy on the outcome of children with VE in China.

Methods: This study retrospectively included 834 children with viral encephalitis who were hospitalized at five medical institutions from 2018 to 2021.

[Effects of Organic Fertilizer Combined with Biochar on Denitrifying Microorganisms and Enzyme Activities in Orchard Soil].

To study the effects of organic fertilizer combined with biochar on soil denitrification and denitrifying microbial community structure, this study took lemon orchard soil as the research object and adopted a pot experiment, setting up five fertilization treatments:no fertilization(CK), conventional fertilization(F), organic fertilizer(P), fertilizer+biochar(FP), and organic fertilizer+biochar(PP). The abundance and community structure of denitrifying microorganisms were studied using real-time quantitative PCR and T-RFLP. Redundancy analysis(RDA) was used to explore the environmental factors affecting the denitrifying microbial community structure, and PLS-PM analysis was used to explore the environmental factors affecting the denitrification potential of lemon orchard soil.

Association of High Plasma Levels of Serpin E1, IGFBP2, and CCL5 With Refractory Epilepsy in Children by Cytokine Profiling.

Inflammatory cytokines participate in the pathology of epilepsy and the development of drug resistance. In this study, we combined a cytokine array and enzyme-linked immunosorbent assay to identify new cytokines in the plasma from children on early stage of the onset of epilepsy (EOE) and children with drug-resistant epilepsy (DRE). Compared with healthy controls, a broad up-regulation of cytokines was observed in patients with EOE, and many of the cytokines were not previously reported.

[Effect of Chemical Fertilizer and Manure Combined with Biochar on Denitrification Potential and Denitrifying Bacterial Community in Rhizosphere Soil].

To clarify the effect of chemical fertilizer and manure combined with biochar on denitrifying microorganisms and denitrification potential of rhizosphere soil, a pot experiment growing lemon was conducted involving five treatments, namely no fertilization (CK), chemical fertilizer (CF), manure (M), chemical fertilizer combined with biochar (CFBC), and manure combined with biochar (MBC). We determined the characteristics of the rhizosphere soil , and type denitrifying bacteria populations; denitrification potential; and soil environmental factors to clarify the effects of chemical and manure combined with biochar on denitrification. Our results showed that compared with that in CK, the CF treatment reduced the rhizosphere soil denitrification potential by 47.

Discrepant diversity patterns and function of bacterial and fungal communities on an earthquake-prone mountain gradient in Northwest Sichuan, China.

Patterns of microbial diversity on elevational gradients have been extensively studied, but little is known about those patterns during the restoration of earthquake-fractured alpine ecosystems. In this study, soil properties, soil enzyme activities, abundance and diversity of soil bacterial and fungal communities at four positions along a 2.6-km elevational gradient in the Snow Treasure Summit National Nature Reserve, located in Pingwu County, Southwest China.

[Effects of Chemical Fertilizer Reduction Substitute with Organic Fertilizer on Soil Functional Microbes and Lemon Yield and Quality].

To clarify the effects of non-rhizosphere/rhizosphere soil functional microbes (nitrifiers, denitrifiers, and phosphorus-solubilizing microorganisms) on lemon yield and quality, the lemon fruit and non-rhizosphere/rhizosphere soil were selected as subjects. To explore the correlation between non-rhizosphere/rhizosphere soil functional microbes and lemon yield and quality under a chemical fertilizer reduction substitute with organic fertilizer, traditional fruit quality determination and multiple molecular techniques were used. The results showed that:① 30% chemical fertilizer reduction substitute with organic fertilizer increased the nitrification intensity and phosphatase activity but effectively controlled the denitrifying enzyme activity.

[Effects of Chemical Fertilizer Reduction Combined with Organic Fertilizer Application on Bacterial Community Structure in Rhizosphere/Non-Rhizosphere Soil of Lemon].

The aim of this study was to examine the effects of different fertilization methods on the physicochemical properties and bacterial community structure of lemon rhizosphere/non-rhizosphere soil in order to provide theoretical basis for scientific and rational fertilization of orchards. A pot experiment was carried out, and six fertilization treatments were set up:control (CK), conventional fertilization (FM), organic fertilizer (P), fresh organic fertilizer (NP), 70% chemical fertilizer+30% organic fertilizer (70FP), and 50% chemical fertilizer+50% organic fertilizer (50FP). Chemical analysis, real-time fluorescence quantitative PCR, and terminal restriction fragment length polymorphism (T-RFLP) were used to study the effects of different fertilization treatments on the physicochemical properties of rhizosphere and non-rhizosphere soils, the abundance of the bacterial 16S rRNA gene, and bacterial community structure.

Ketogenic diet therapy in children with epilepsy caused by SLC2A1 mutations: a single-center single-arm retrospective study.

Background: This retrospective study assessed the efficacy and safety of ketogenic diet therapies in children with epilepsy caused by SLC2A1 genetic mutations and glucose transporter type 1 deficiency syndrome.

Methods: Pediatric patients with epilepsy symptoms admitted to our medical center between January 2017 and October 2021 were included if they presented with an SLC2A1 genetic mutation on whole-exome sequencing. We analyzed the patients' convulsions and treatment with antiepileptic drugs.

Ultrasound-assessed diaphragm dysfunction predicts clinical outcomes in hemodialysis patients.

Skeletal muscle atrophy is prevalent and remarkably increases the risk of cardiovascular (CV) events and mortality in hemodialysis (HD) patients. However, whether diaphragm dysfunction predicts clinical outcomes in HD patients is unknown. This was a prospective cohort study of 103 HD patients.

Highly sensitive fluorescent detection of EDIL3 overexpressed exosomes for the diagnosis of triple-negative breast cancer.

EDIL3 is a strong and highly accurate diagnostic marker for breast cancer, meanwhile, EDIL3 overexpressed exosomes are novel biomarkers for the early diagnosis of triple-negative breast cancer (TNBC). Here, we proposed a fluorescent detection method for EDIL3 overexpressed exosomes, which is simple and sensitive. Basically, we utilized a magnetic nanospheres (MNS) based liquid sandwich immunoassay strategy.

Sleep Disturbances in Chinese Children with Epilepsy: Associations with Behavioral Problems and Quality of Life.

Objective: To investigate the association between sleep disturbances and behavioral problems as well as quality of life (QOL) in Chinese children with epilepsy.

Methods: Caregivers of 167 epileptic children aged 3 to 12 years completed the Child Sleep Habits Questionnaire (CSHQ), the Strengths and Difficulties Questionnaire (SDQ), and the Pediatric Quality of Life Inventory (PedsQL™, 4.0 Core).

[Responses of Soil and Gene Microbial Communities to the Combined Application of Biochar with Chemical Fertilizers and Organic Fertilizers].

Soil microorganisms have an important influence on the transformation of soil nutrients. As functional genes encoding phosphatase, and provide effective means for detecting the types, abundance, and community structure of microorganisms in the environment, and studying the changes in the diversity of and gene microbial communities in the rhizosphere and non-rhizosphere soil of the plant rhizosphere and non-rhizosphere soil under the treatment of chemical fertilizer and organic fertilizer combined with biochar can provide a scientific basis for the agricultural utilization of biochar. In this study, corn stalks and rice husk stalks were used as test materials, and the pot experiment method was used to set the following treatments:control (CK), traditional fertilization (F), chemical fertilizer+20 t·hm rice husk biochar (FP), chemical fertilizer+10 t·hm rice husk biochar+10 t·hm corn biochar (FPM), organic fertilizer+20 t·hm rice husk biochar (PP), and fresh organic fertilizer+20 t·hm rice husk biochar (NPP).

[Effect of Combined Application of Biochar with Chemical Fertilizer and Organic Fertilizer on Soil Phosphatase Activity and Microbial Community].

To study changes in phosphatase activity, we examined the diversity of and gene microbial communities in the rhizosphere and non-rhizosphere soil of plants under the treatment of chemical fertilizer and organic fertilizer combined with biochar. These results can provide a certain theoretical guidance for the conversion of insoluble phosphorus in the soil phosphorus pool to the inorganic phosphate ion that can be absorbed by plant roots and also provide a certain experimental basis for the improvement of the availability of phosphorus in the soil and the agricultural utilization of biochar. In this study, corn stalks and rice husk stalks were used as test materials, and the pot experimental method was adopted using the following treatments:set control (CK), traditional fertilization (F), chemical fertilizer+20 t·hm rice husk biochar (FP), chemical fertilizer+10 t·hmrice husk biochar+10 t·hm corn biochar (FPM), organic fertilizer+20 t·hm rice husk biochar (PP), and fresh organic fertilizer+20 t·hm rice husk biochar (NPP).

[Response of Soil Organic Carbon Content in Different Slope Positions to Fertilization Management in Purple Soil Sloping Fields].

The purple soil sloping field is the main cultivated land type in the Three Gorges area, and the soil fertility directly determines crop yield. In order to explore the effects of different fertilization treatments on the soil organic carbon content at different slope positions, field experiments were carried out at the Three Gorges Reservoir Test Station of Chengdu Institute of Mountain and Disasters, Chinese Academy of Sciences. A total of five treatments were set up:no fertilization(CK), conventional fertilization(T), optimum fertilization(T), biochar combined with 85% of T(T), and straw combined with 85% of T(T), to study the differences in soil aggregate composition, soil total organic carbon, soluble organic carbon, and microbial carbon content at different slope positions under different fertilization treatments.

[Effects of Biochar and Straw Return on Soil Aggregate and Organic Carbon on Purple Soil Dry Slope Land].

The aim of the study was to understand the impact of biochar and straw return on soil aggregates and organic carbon for soil improvement of the newly cultivated purple soil dry slope land in the Three Gorges Reservoir area. In this study, a field test was used to set five treatment pairs with regards to soil aggregate composition and organic carbon distribution:no fertilization(CK), conventional fertilization(NPK), optimized fertilization(GNPK), chemical fertilizer reduction combined with straw(RSD), and chemical fertilizer reduction combined with biochar(BC). The results showed that fertilization can improve the level of soil fertility, especially with the RSD and BC treatments.