Multi-omics characterization of autophagy-related molecular features in Intrahepatic cholestasis of pregnancy†.

Introduction: Despite the increasing body of evidence that autophagy implicate in Intrahepatic cholestasis of pregnancy (ICP), we are still far from a mechanistic understanding of the autophagy in ICP pathogenesis.

Methods: In this study, we performed untargeted lipidomics, scRNA-seq, and RNA-seq data to systematically demonstrate the lipid alterations, cells and gene expression closely related to autophagy in placenta. These results were also verified by tissue, cell, and animal experiments.

Photocatalytic synthesis of hydrogen peroxide: recent advances, challenges, and future perspectives.

Hydrogen peroxide (HO), an eco-friendly oxidant and multifunctional chemical feedstock, is widely used in medical disinfection, paper bleaching, green chemistry, and environmental management. However, the traditional industrial anthraquinone process, which involves high energy consumption and causes severe environmental pollution, is becoming increasingly unsuitable for the rising demand for sustainable and eco-friendly production methods. In response, several innovative production strategies have been developed.

Global, regional, and national epidemiology of otitis media in children from 1990 to 2021.

Background: Otitis media(OM) is a prevalent ear condition, particularly among children, with a significant impact on quality of life. This study aimed to elucidate the global prevalence and disability-adjusted life years (DALYs) associated with OM in the 0-14 age group from 1990-2021, using data from the 2021 Global Burden of Disease (GBD) study.

Methods: Our study conducted a comprehensive analysis of OM data from the GBD 2021 report, examining the prevalence and DALYs related to OM across 204 countries and territories over a 32-year period.

HM108 alleviates obesity in rats fed a high-fat diet by modulating the gut microbiota, metabolites, and inhibiting the JAK-STAT signalling pathway.

Introduction: Obesity is a globally prevalent metabolic disease, and high-calorie diets are major contributors to its development. Probiotic interventions can modulate the gut flora and alleviate systemic and low-grade inflammation, making them potential in-terventions for alleviating metabolic syndrome.

Methods: This study explored the beneficial effects of the HM108 strain derived from breast milk on obesity in high-fat diet-induced rats using a multi-gradient concentration in-tervention.

HM018 derived from breast milk ameliorates hyperlipidemia in high-cholesterol rats by modulating bile acid metabolism.

Introduction: Hyperlipidemia, a prevalent metabolic disorder with rising global incidence, has become a major public health concern. Probiotics allow for a mild intervention strategy for hyperlipidemia management that has garnered increasing attention.

Methods: In this study, we investigated the therapeutic effects and underlying mechanisms of HM018 in hypercholesterolemic rats.

Erigoster B targeting DECR1 induces ferroptosis of breast cancer cells via promoting phosphatidylcholine/arachidonic acid metabolism.

Breast cancer, the leading cause of death in females, is profoundly affected by cooperative changes of molecular and pathological characters in fatty acid metabolism. To investigate the core molecule of fatty acid metabolism in breast cancer progression, we performed in-silico analyses and found 2,4-Dienoyl-CoA reductase (DECR1) was overexpressed in breast cancer tissues with impaired prognoses of patients. In vitro experiments showed that the proliferation and migration of breast cancer cells were significantly inhibited after DECR1 knockdown.

Comparison of endoscopic vacuum therapy with standard care for colorectal anastomotic leakage: a systematic review and meta-analysis.

This systematic review and meta-analysis aimed to evaluate the effectiveness of endoscopic vacuum therapy (EVT) compared with standard care in managing colorectal anastomotic leakage, a serious complication following colorectal surgery.We conducted a literature search across PubMed, Embase, and the Cochrane Library for clinical studies published before October 2024 that compared the outcomes of EVT and standard care for anastomotic leakage, including leakage resolution rates, treatment duration, hospital stay duration, or postoperative complications. The pooled estimates of risk ratio (RR) or mean difference were primarily derived using a fixed-effects model, with a random-effects model used when high heterogeneity was detected.

Comprehensive overview of antibody drug-related clinical studies in gynecology: insights from ClinicalTrials.gov.

Antibodies have been widely used globally over the past decade and play an increasingly important role in modern medicine. Notably, significant advancements have been achieved in the realm of gynecology, particularly in gynecological cancers. This study endeavors to present a thorough overview of antibody-related drug clinical studies in gynecology registered on ClinicalTrials.

Comparative Analysis of Growth Dynamics and Relative Abundances of Gut Microbiota Influenced by Ketogenic Diet.

Unlabelled: Although the compositional alterations of gut bacteria in ketogenic diet (KD) have been intensively investigated, the causal relationship between this extreme diet and the microbiota changes is not fully understood. Here, we studied the growth dynamics of intestinal bacteria in KD. We used the CoPTR method to calculate the peak-to-trough ratio (PTR) based on metagenomic sequencing data, serving as an indicator of bacterial growth rates.

Systems metabolic engineering of Escherichia coli for the high-level production of deoxyviolacein, a natural colorant.

Deoxyviolacein is a natural colorant with various biological properties, widely applied in cosmetic and pharmaceutical fields. However, current methods of deoxyviolacein production by natural producers may cause highly lethal infections in humans, limiting the sustainable production of deoxyviolacein. Here, an l-tryptophan-producing Escherichia coli strain was engineered for efficient deoxyviolacein production.

A Fiber-Reinforced Poly(ionic liquid) Solid Electrolyte with Low Flammability and High Conductivity for High-Performance Lithium-Metal Batteries.

Construction of polymer-based solid electrolytes with both low flammability and high ionic conductivity for lithium-metal batteries is still a great challenge but highly desirable. Herein, we report on a series of fiber-reinforced poly(ionic liquid) solid electrolytes prepared through an copolymerization of ionic liquid monomers (IL) and poly(ethylene glycol) diacrylate (PEGDA) units with different ratios inside a polyacrylonitrile (PAN) fiber membrane. Such PAN/Poly-IL-PEGDA composite electrolytes demonstrate promising low flammability due to the excellent fire-resistant feature of the employed IL units.

The Value of P16 Protein Detection in the Screening for High-Grade Squamous Intraepithelial and Higher Lesions of the Cervix by the Combined Detection of HR-HPV and TCT.

Liquid-based thin layer cytology (TCT) and HR-HPV detection are the most important screening methods for cervical cancer. These two methods have limited sensitivity and specificity, so some cervical lesions are still missed or misdiagnosed. This paper mainly discusses the value of P16 protein detection in cervical cancer screening.

Enhanced Microwave Absorption of Multi-Interface Core-Shell FeSiAl@MnO@C Composites by Morphology Engineering.

The rational manipulation of interfacial properties, composition, and morphology of materials has emerged as an effective strategy for enhancing their microwave absorption performance. The crystal phase composition, microstructure, and electromagnetic properties can be regulated by varying the thermal treatment temperature. The synergistic integration of heterogeneous interfaces, magnetic materials, microstructures, and defect engineering helps optimize impedance matching and enhance polarization and magnetic losses.

Eco-Friendly high Drug-Loading microemulsions with Incorporation of Deep eutectic Solvents: Advancing precision with the dual Ouzo effect.

Deep eutectic solvents (DES) enhance drug solubility but require delivery systems, while the Ouzo effect enables surfactant-free microemulsion formation despite limitations in oil phase ratio. By integrating DES as the oil phase, this study develops a dual Ouzo effect microemulsion system that induces both microemulsions and nanoprecipitations simultaneously. Through detailed analysis of composition diagrams, precise adjustment of the mass ratio of VA64 to propylene glycol enables strict control over particle size from 200 nm to 550 nm.

Deaggregation of micronized insoluble drugs by incorporating mannitol form α.

Micronization is frequently employed to increase the dissolution of poorly soluble drugs, but it easily led to powder aggregation and difficult to mix well on the micro level with poor content uniformity and erratic dissolution behavior. Mannitol is the most commonly used pharmaceutical excipient, and its β form (β-mannitol) is commercially available and extensively investigated, whereas form α (α-mannitol) remain poorly understood. Here, this study demonstrated that α-mannitol could significantly eliminate aggregation phenomena of micronized drugs (i.

Acupoint Catgut Embedding Therapy in Traditional Chinese Medicine for Managing Allergic Rhinitis.

Acupuncture Point Embedding Therapy is a comprehensive treatment method that combines modern biomaterials with acupuncture techniques. Acupoint catgut embedding (ACE) not only avoids the adverse reactions associated with Western medicine but also incorporates the benefits of traditional acupuncture, extending the duration of its therapeutic effects. Allergic rhinitis (AR) is a condition known for its challenging treatment and tendency to persist.

The landscape of recurrent spontaneous abortion registered on clinical trials.gov.

Objective: Recurrent spontaneous abortion (RSA) presents a significant challenge in the field of reproductive medicine, as effective treatments remain limited despite extensive research efforts. A comprehensive understanding of current RSA clinical trials is essential for enhancing trial design and identifying existing research gaps. The aim of this study is to characterize RSA related clinical trials registered on Clinical Trials.

MOTS-c regulates the ROS/TXNIP/NLRP3 pathway to alleviate diabetic cardiomyopathy.

Chronic low-grade inflammation is a characteristic of diabetes, which often culminates in cardiovascular events including myocardial damage, thereby increasing the risk of debilitating cardiac complications. The mitochondria-derived peptide MOTS-c regulates glucose and lipid metabolism while improving insulin resistance, making it a potential candidate for the treatment of diabetes and cardiovascular diseases. We investigated the impact of MOTS-c on cardiac structure and inflammation in diabetic rats induced by a high-sugar-fat diet combined with low-dose streptozotocin (30 mg/kg, i.

Deodorization mechanism of the main aroma compounds on the fishy odor in boiled fish during heating.

A boiled fish simulation system was constructed to explore the deodorization effect of main aroma compounds (MAC) on myofibrillar protein (MP) with main fishy compounds (MFC) during heating. The results showed that the MFC content of boiled fish was reduced by 63.10-78.

Visual and Quantitative Analysis of Dietary Fiber-Microbiota Interactions via Metabolic Labeling In Vivo.

Dietary fiber (DF)-based interventions are crucial in establishing a health-promoting gut microbiota. However, directly investigating DFs' in vivo interactions with intestinal bacteria remains challenging due to the lack of suitable tools. Here, we develop an in vivo metabolic labeling-based strategy, which enables not only imaging and identifying the bacteria that bind with specific DF in the intestines, but also quantifying DF's impact on their metabolic status.

Improving the Genome Editing Efficiency of CRISPR/Cas9 in Melon and Watermelon.

CRISPR/Cas9 is a powerful genome editing tool for trait improvement in various crops; however, enhancing mutation efficiency using CRISPR/Cas9 in watermelon and melon remains challenging. We designed four CRISPR systems with different sgRNA expression cassettes to target the phytoene desaturase () gene in melon. The constructed vectors were delivered to host plants using -mediated transformation.

CircABCC1 reduces endometrial receptivity via METTL3/FAM155B axis.

Objective: Impaired endometrial receptivity is the main cause of embryo implantation failure. Little information is available on the role of circRNAs in endometrial receptivity. Here, the effect of circABCC1 on endometrial receptivity and its mechanism were investigated.