A Self-Adaptive Rhodium(I) Complex-Based Nanoplatform with Type II Immunogenic Cell Death for Near-Infrared Phosphorescence Imaging and Cancer Immunotherapy.

Despite immunogenic cell death (ICD) has garnered significant attention in cancer therapy, achieving precise in vivo immunity activation and simultaneous visualization of immunotherapy processes remain significant challenges due to the difficulties in facile integration of multifunctionalities in a single nanomedicine. For this purpose, herein a self-adaptive rhodium(I) complex-based nanoplatform driven by metallophilic interactions is reported not only for near-infrared (NIR) imaging-guided cancer immunotherapy, but also as the first example of a rhodium(I)-based ICD inducer. Specifically, this nanoplatform enables high tumor enrichment by utilizing homologous targeting capability camouflaged by cancer cell membranes and facilitates enhanced in vivo NIR phosphorescence imaging.

Catalyst- and Base-Free Synthesis of Pyridine-Fused Uracils from 6-Methyluracils, Aldehydes, and Ammonium Iodide via a One-Pot Multicomponent Reaction.

A novel multicomponent strategy for the efficient formation of pyridine-fused uracils from 6-methyluracils, aldehydes, and ammonium iodide has been developed. This protocol employs ammonium iodide as an environmentally friendly nitrogen source and requires no additional catalysts or bases while providing excellent yields, high atom economy, and broad functional group compatibility.

Synthesis of an amphiphilic bi-prodrug based on gossypol and cytarabine for drug self-delivery.

Drug-drug conjugates have become a research hotspot in nanomedicine. An amphiphilic assembled drug-drug conjugate can achieve drug self-delivery without any carriers and change the distribution of free drugs. Herein, we synthesized a pH-responsive bi-prodrug of gossypol (GP) and cytarabine (Ara-C) for cancer therapy.

Clinical Efficacy of Gua Sha Therapy Combined With Acupoint Application on Chronic Cough in Pediatrics.

Objective: To investigate the clinical efficacy of gua sha therapy combined with acupoint application on pediatric chronic cough.

Methods: The convenience sampling method was used to enroll 200 children with chronic cough from a hospital in Guangzhou between April 2023 and October 2024 in this study. These children were divided into a conventional control group, an acupoint patch group, a gua sha group, and a gua sha therapy combined with an acupoint patch treatment group (combined group).

Accelerated sludge granulation of novel complete ammonium and nitrate removal via denitratation anammox over nitrite process at elevated loading rates.

The Complete Ammonium and Nitrate Removal via Denitratation Anammox Over Nitrite (CANDAN) process was evaluated for rapid sludge granulation in a lab-scale sequencing batch reactor. Over 119 days under increasing nitrogen loading rates (NLRs), the system finally achieved average 89.2 % total nitrogen removal at 1.

Nuclease-Mimetic Nanomaterials: From Fundamentals to Bioapplications.

With the rapid development of nanozymes and nanomedicine, designing novel nanostructures directly acting on deoxyribonucleic acid (DNA) has great therapeutic potential because DNA is the carrier of genetic information and plays a vital role on life activities of the organism. Specifically, DNA cleavage is an important step in most of these DNA engineering technologies. While nucleases play crucial roles in the cell metabolism by efficient DNA cutting, the practical applications of natural nucleases suffer from some intrinsic shortcomings such as high cost and intolerance to harsh environments.

Effect of carbon materials with different nano-iron oxide loadings on aerobic granular sludge systems.

This study investigated iron oxide-loaded powdered activated carbon (FONP-PAC) with varying Fe/C ratios (FC1 in S1, FC2 in S2) in aerobic granular sludge (AGS) systems. S2 achieved the fastest sludge growth, reaching 1106 μm by day 80 (87.14% larger than control S0).

A robust metal-organic framework-based nanozyme with multienzyme-like properties for synergistic tumor therapy via efficient and sustainable Nitric oxide generation.

Nitric oxide (NO)-based gas therapy represents an emerging strategy for cancer treatment, which, however, still suffers from insufficient intracellular NO production for compromised therapeutic efficiency due to limited endogenous hydrogen peroxide (HO) concentration and relatively slow NO generation rate. The use of metal-organic framework (MOF) with highly ordered porous structure and functional adjustability to design a multienzyme-like nanozyme provides a simple yet reliable approach for efficient and sustainable NO generation. Herein, a MOF-based nanozyme with multienzyme-like properties is constructed by depositing ultrasmall gold nanoparticles (Au NPs) and subsequently loading a NO donor, l-arginine (l-Arg) on an iron porphyrin integrated MOF, which enables efficient and sustainable NO generation for synergistic tumor therapy.

Konjac glucomannan-based foams incorporating cellulose phase change microcapsules for efficient thermal energy regulation.

Biomass foam with porous structure has broad application prospects in thermal energy management. However, traditional foams can only passively insulate heat, unable to effectively store thermal energy and prolong the insulation time. In this work, microcapsules rich in paraffin were prepared using the Pickering emulsion template method with phosphorylated cellulose nanocrystals (CNC) as an emulsifier.

Konjac glucomannan foams integrated with bilayer phase change microcapsules for efficient heat storage and thermal insulation.

The traditional foams can only block heat loss, and cannot effectively store and release heat energy on demand to extend the insulation time. In this work, the paraffin-rich monolayer microcapsules were prepared using negatively charged phosphorylated cellulose nanofibers (CNF) as the emulsifier of Pickering emulsion. The positive chitosan was assembled on the surface of the monolayer microcapsules through an electrostatic layer-by-layer self-assembly method to prepare the bilayer microcapsules.

Legacy effects of precipitation change: Theories, dynamics, and applications.

The intensification of climate-induced precipitation change poses a dual challenge to terrestrial ecosystems: immediate effects on their structure and function, coupled with legacy effects that persist beyond the cessation of precipitation change. Quantifying these legacy effects accurately can greatly assist in assessing the long-term impact of precipitation change. However, their broader understanding is just beginning.

APC orchestrates microtubule dynamics by acting as a positive regulator of KIF2A and a negative regulator of CLASPs.

Tumor suppressor protein Adenomatous polyposis coli protein (APC) is an EB-binding and microtubule (MT) plus end-tracking protein; however, how exactly APC regulates MT dynamics remains elusive. Here, we show that in LLC-PK1 cells, APC and KIF2A, an MT depolymerase, form a complex clustering at the cell edge and destabilize MTs at the MT plus ends. Further biochemical characterization and mutational analysis reveal key residues for the APC-KIF2A interaction.

Deodorization mechanism of the main aroma compounds on the fishy odor in boiled fish during heating.

A boiled fish simulation system was constructed to explore the deodorization effect of main aroma compounds (MAC) on myofibrillar protein (MP) with main fishy compounds (MFC) during heating. The results showed that the MFC content of boiled fish was reduced by 63.10-78.

Recent progress of natural materials-based hydrogel for postoperative chemotherapy.

Hydrogels have been widely used for in situ postoperative chemotherapy due to fewer side effects and longer duration of drug action compared to systemic chemotherapy. This paper reviews the application of natural materials-based hydrogels after tumor resection to explore them as an option for postoperative chemotherapy. Different material-based hydrogels, different response-based hydrogels, and the clinical applications of implantable, injectable, and sprayed hydrogels were investigated and summarized.

Anaerobic digestion and biochar/hydrochar enhancement of antibiotic-containing wastewater: Current situation, mechanism and future prospects.

The increasing consumption of antibiotics by humans and animals and their inappropriate disposal have increased antibiotic load in municipal and pharmaceutical industry waste, resulting in severe public health risks worldwide. Anaerobic digestion (AD) is the main force of antibiotic-containing wastewater treatment, and the adaptability of biochar/hydrochar (BC/HC) makes it an attractive addition to AD systems, which aim to promote methane production efficiency. Nevertheless, further studies are needed to better understand the multifaceted function of BC/HC and its role in antibiotic-containing wastewater AD.

Preparation of Glutathione-Responsive Paclitaxel Prodrug Based on Endogenous Molecule of L-Glutathione Oxidized for Cancer Therapy.

Using an endogenous carrier is the best method to address the biocompatibility of carriers in the drug delivery field. Herein, we prepared a glutathione-responsive paclitaxel prodrug micelle based on an endogenous molecule of L-glutathione oxidized (GSSG) for cancer therapy using one-pot synthesis. The carboxyl groups in L-glutathione oxidized were reacted with the hydroxyl group in paclitaxel (PTX) using the catalysts dicyclohexylcarbodiimide (DCC) and 4-dimethylaminopyridine (DMAP).

Etomoxir-carnitine, a novel pharmaco-metabolite of etomoxir, inhibits phospholipases A and mitochondrial respiration.

Mitochondrial fatty acid oxidation serves as an essential process for cellular survival, differentiation, proliferation, and energy metabolism. Numerous studies have utilized etomoxir (ETO) for the irreversible inhibition of carnitine palmitoylcarnitine transferase 1 (CPT1), which catalyzes the rate-limiting step for mitochondrial long-chain fatty acid β-oxidation to examine the bioenergetic roles of mitochondrial fatty acid metabolism in many tissues in multiple diverse disease states. Herein, we demonstrate that intact mitochondria robustly metabolize ETO to etomoxir-carnitine (ETO-carnitine) prior to nearly complete ETO-mediated inhibition of CPT1.

A Programmable Microfluidic Paper-Based Analytical Device for Simultaneous Colorimetric and Photothermal Visual Sensing of Multiple Enzyme Activities.

New strategies for the simultaneous and portable detection of multiple enzyme activities are highly desirable for clinical diagnosis and home care. However, the methods developed thus far generally suffer from high costs, cumbersome procedures, and heavy reliance on large-scale instruments. To satisfy the actual requirements of rapid, accurate, and on-site detection of multiple enzyme activities, we report herein a smartphone-assisted programmable microfluidic paper-based analytical device (μPAD) that utilizes colorimetric and photothermal signals for simultaneous, accurate, and visual quantitative detection of alkaline phosphatase (ALP) and butyrylcholinesterase (BChE).

Establishment and application of a rapid visualization method for detecting nucleic acid.

Background: Swift and accurate detection of , which is a prominent causative pathogen associated with seafood contamination, is required to effectively combat foodborne disease and wound infections. The gene is relatively conserved within and is primarily involved in the expression and regulation of virulence genes with a notable degree of specificity. The aim of this study was to develop a rapid, simple, and constant temperature detection method for in clinical and nonspecialized laboratory settings.

DNA-based ForceChrono probes for deciphering single-molecule force dynamics in living cells.

Understanding cellular force transmission dynamics is crucial in mechanobiology. We developed the DNA-based ForceChrono probe to measure force magnitude, duration, and loading rates at the single-molecule level within living cells. The ForceChrono probe circumvents the limitations of in vitro single-molecule force spectroscopy by enabling direct measurements within the dynamic cellular environment.

Changes in Microbial Composition During the Succession of Biological Soil Crusts in Alpine Hulun Buir Sandy Land, China.

Biological soil crusts (biocrusts) are considered "desert ecosystem engineers" because they play a vital role in the restoration and stability maintenance of deserts, including those cold sandy land ecosystems at high latitudes, which are especially understudied. Microorganisms participate in the formation and succession of biocrusts, contributing to soil properties' improvement and the stability of soil aggregates, and thus vegetation development. Accordingly, understanding the composition and successional characteristics of microorganisms is a prerequisite for analyzing the ecological functions of biocrusts and related applications.

Current advances in modulating tumor hypoxia for enhanced therapeutic efficacy.

Hypoxia is a common feature of most solid tumors, which promotes the proliferation, invasion, metastasis, and therapeutic resistance of tumors. Researchers have been developing advanced strategies and nanoplatforms to modulate tumor hypoxia to enhance therapeutic effects. A timely review of this rapidly developing research topic is therefore highly desirable.

Rapid visual nucleic acid detection of Vibrio alginolyticus by recombinase polymerase amplification combined with CRISPR/Cas13a.

Vibrio alginolyticus (V. alginolyticus) is a common pathogen in the ocean. In addition to causing serious economic losses in aquaculture, it can also infect humans.