CaMutQC: An R package for integrative quality control and filtration of cancer somatic mutations.

The quality control and filtration of cancer somatic mutations (CAMs), including the elimination of false positives due to technical bias and the selection of key mutation candidates, are crucial steps for downstream analysis in cancer genomics. However, due to diverse needs and the lack of standardized filtering criteria, the filtering strategies applied vary from study to study, often resulting in reduced efficiency, accuracy, and reproducibility. Here, we present CaMutQC, a heuristic quality control and soft-filtering R/Bioconductor package designed specifically for CAMs.

Clinical features and laboratory indicators of dengue infection in China: a retrospective study of adult patients in a hospital of traditional Chinese medicine.

Background: Dengue is an arboviral disease caused by the dengue virus, primarily transmitted by mosquitoes in tropical and subtropical regions. Despite preventive measures, the incidence and mortality of dengue remain significant. While the acute phase of infection often presents with mild, self-limiting symptoms and may mimic other undifferentiated febrile illnesses, the risk of mortality is particularly high during the acute phase of secondary infections, which are associated with more severe clinical manifestations.

One versus many independent assemblies of symbiotic nitrogen fixation in flowering plants.

Some species of legumes and nine other flowering plant families form symbioses with bacteria that fix atmospheric nitrogen within specialized plant structures called nodules. How and how often nodulation symbiosis originated has implications for engineering symbiotic nitrogen fixation in non-legume crops. The prevailing hypothesis of a single origin with massive parallel losses has been challenged in a phylogenomic study favoring 16 origins and 10 losses.

Identification Mechanisms of Luteolin Improve the Storability and Resistance to in Tomato.

Tomatoes are an important vegetable/fruit consumed worldwide. The lack of effective storage methods causes high postharvest losses, which constrain tomato production. In this study, the potential of luteolin, an important flavonoid, as a preservative to increase the quality of tomato and resistance during postharvest storage was assessed, and its extraction process was optimized.

Evaluating antibiotic use patterns and compliance in Shanxi province hospitals: a 7-year retrospective study of national clinical improvement system data (2015-2021).

Objective: With the increasing prevalence of antimicrobial resistance (AMR), the rational use of antibiotics is crucial. This study aimed to evaluate the antibiotic use patterns and influencing factors of compliance in Shanxi Province Hospitals between 2015 and 2021 and provide data support for the management of antibiotics.

Design: This was a retrospective observational study of trends in antibiotic use and data reporting trends in the context of antimicrobial stewardship (AMS).

Preparation, Physicochemical Properties, Biological Activity of a Multifunctional Composite Film Based on Zein/Citric Acid Loaded with Grape Seed Extract and Its Application in Solid Lipid Packaging.

Development of bio-based active packaging systems for lipid stabilization presents critical importance in preserving lipid integrity and ensuring food safety. Zein/citric acid (Z/CA) composite films containing grape seed ethanol extract (GSEE) (0-8% ) were prepared by the solvent casting method. The structural, functional, and environmental properties of the films, including physical and chemical properties, mechanical properties, antioxidant capacity, antibacterial activity, oxidation inhibition effect, and biodegradability, were comprehensively characterized and evaluated.

Phase transition of WTAP regulates mA modification of interferon-stimulated genes.

-methyladenosine (mA) is the most prevalent modification of mRNA which controls diverse physiological processes. Although mA modification has been reported to regulate type I interferon (IFN) responses by targeting the mRNA of IFN-β and the interferon-stimulated genes (ISGs), the detailed mechanism of how mA methyltransferase complex (MTC) rapidly responds to conduct the modification on nascent mRNA during IFN-β stimulation remains largely unclear. Here, we demonstrate that WTAP, the adaptor protein of mA MTC, undergoes dephosphorylation-regulated phase transition from aggregates to liquid-like condensates under IFN-β stimulation, thereby mediating mA modification of a subset of ISGs to restrict their expression.

M6Allele: a toolkit for detection of allele-specific RNA N6-methyladenosine modifications.

Background: Allelic gene-specific regulatory events are crucial mechanisms in organisms, pivotal to many fundamental biological processes such as embryonic development and chromosome inactivation. Allelic gene imbalance manifests at both RNA expression and epigenetic levels. Recent research has unveiled allelic-specific regulation of RNA N6-methyladenosine (m6A), emphasizing the need for its precise identification.

Microsurgery Versus Embolization for Spinal Cord Arteriovenous Malformations: A Proposed Grading System.

Background And Objectives: Comparing microsurgery and embolization for spinal cord arteriovenous malformations (SCAVMs) is challenging because of the disease's rarity and the highly heterogeneous angioarchitecture. The aim of this study was to compare outcomes between microsurgery and embolization using a grading system for SCAVMs that effectively stratifies angioarchitectural complexities.

Methods: A total of 714 patients were included, with 308 undergoing microsurgery.

Influence of Onion Peel Extract on the Dough Characteristics of High-Gluten Wheat Flour and the Quality of Bread.

In this study, we evaluated the effect of onion peel extract (OPE), which is rich in phenolics and flavonoids, on the performance of high-gluten wheat flour and bread quality to meet consumer demand for functional bakery products. The addition levels of OPE were set at 0%, 0.25%, 0.

Physicochemical and microstructural characteristics of canola meal fermented by autonomously screened Bacillus licheniformis DY145 and its immunomodulatory effects on gut microbiota.

Canola meal (CM), a variety of double-low rapeseed meal (RSM), is a valuable protein source due to its reduced glucosinolate (<20 μmol/L) and erucic acid (<2 %) content. In this study, bioactive small peptides were derived from CM through fermentation with an autonomously screened Bacillus licheniformis DY145 strain. Strain mutagenesis and fermentation condition optimization further enhanced peptide activity.

Enhancement of Emulsifying Activity in Soy-Protein-Based Products by Partial Substitution with Zein Hydrolysates and Transglutaminase Addition.

Partially substituting other proteins in soy-protein-based products is an effective method to meet nutritional and application requirements. However, the emulsifying properties of soybean protein isolates (SPI) when partially substituted with zein hydrolysates (ZH) remain unknown. In the present work, protein blend (0 h-SPI/ZH) from SPI and ZH with a ratio of 3.

Doped-free structure is used to realize organic light-emitting devices with low blue harm, low color temperature, and high color rendering index.

With the development of light-emitting devices, researcher gradually shift their attention from the efficiency of the devices to the health of lighting sources. Herein, we first designed a low correlated color temperature (CCT) and high color rendering index (CRI) spectrum with minimal blue light accession, which is beneficial to reduce the inhibitory effect of melatonin secretion. Besides, the spectrum had suitable near-infrared light with a wavelength of 670-810 nm, which could promote cellular metabolism, migration, and value-added, achieving more comfortable and healthy lighting.

Cancer immunotherapy by silencing transcription factor c-Rel using peptide-based nanoparticles.

Background: Cancer immunotherapy has shown promising results in the clinic, but it faces great challenges such as low response rates and low efficacy in solid tumors. c-Rel, a member of the nuclear factor (NF)-κB family, is a newly described immune checkpoint for myeloid-derived suppressor cells (MDSCs), which contribute to the formation of immune-suppressive tumor microenvironment and resistance to cancer immunotherapy. How to selectively target myeloid c-Rel for the treatment of cancer is not well established.

GOAT: a novel global-local optimized graph transformer framework for predicting student performance in collaborative learning.

Collaborative learning is a prevalent learning method, and modeling and predicting student performance in such paradigms is an important task. Most current methods analyze this complex task solely based on the frequency of student activities, overlooking the rich spatial and temporal features present in these activities, as well as the diverse textual content provided by various learning artifacts. To address these challenges, we choose a software engineering course as the study subject, where students are required to team up and complete a software project together.

Enhanced Thermal and Storage Stability of Glucose Oxidase via Encapsulation in Chitosan-Coated Alginate and Carboxymethyl Cellulose Gel Particles.

Glucose oxidase (GOD) is widely used as an important oxidoreductase in various fields. However, maintaining the vitality and stability of GOD under environmental stress is a challenge. To improve the thermal and storage stability of GOD, this study constructed sodium alginate-carboxymethyl cellulose sodium gel particles (SA/CMC) and chitosan-coated SA/CMC gel particles (CS/SA/CMC) of GOD.

Ultrasound-assisted preparation of wax-based composite gelator: Structural characterisation, in vitro antioxidant activity and application in oleogels.

In recent years, the development of zero-trans fatty acid products instead of traditional hydrogenated and high-unsaturated fatty acid animal and vegetable oils has been an increasing interest in the field of food. This paper focused on the ultrasound-assisted preparation of a novel wax-based composite gelator loaded with natural antioxidant to prepare oleogels with good storage oxidation stability. The preparation of the wax-based composite gelator was to first form the anthocyanin (ACNs) and soyabean lecithin (SL) complex, and then homogenized with beeswax (BW).

Clinical, genomic, and histopathologic diversity in cerebral cavernous malformations.

Cerebral cavernous malformations (CCMs) are hemorrhagic vascular disorders with varied clinical and radiological presentations, occurring sporadically due to MAP3K3 or PIK3CA mutations or through inherited germline mutations of CCM genes. This study aimed to clarify the clinical, genetic, and pathological features of CCMs using a multicenter cohort across three Chinese centers. We analyzed 290 surgical specimens from symptomatic CCM patients, utilizing whole-exome sequencing, droplet digital PCR, and targeted panel sequencing, alongside immunohistology to examine genotypic and phenotypic differences.

DSCI: a database of synthetic biology components for innate immunity and cell engineering decision-making processes.

Although significant progress of clinical strategy has been made in gene editing and cell engineering in immunotherapy, it is now apparent that design and modification in terms of complex signaling pathways and motifs on medical synthetic biology are still full of challenges. Innate immunity, the first line of host defense against pathogens, is critical for anti-pathogens immune response as well as regulating durable and protective T cell-mediated anti-tumor responses. Here, we introduce DSCI (Database of Synthetic Biology Components for Innate Immunity, https://dsci.

Changes in the Flavor of Cold-Pressed Tiger Nut Oil ( L.) During Refining Processes and Evaluation of Its Thermal Properties.

Oil extracted from tiger nut is a good, edible source owing to its richness in unsaturated fatty acids. This study investigated the effects of the refining processes on the flavor components of crude tiger nut oil by GC-MS and focused on the thermal stability of the refined oil under high-temperature conditions. Three different refining processes were evaluated: citric acid-assisted hydration degumming, alkali deacidification and bleaching.

Chemically engineered antibodies for autophagy-based receptor degradation.

Cell surface receptor-targeted protein degraders hold promise for drug discovery. However, their application is restricted because of the complexity of creating bifunctional degraders and the reliance on specific lysosome-shuttling receptors or E3 ubiquitin ligases. To address these limitations, we developed an autophagy-based plasma membrane protein degradation platform, which we term AUTABs (autophagy-inducing antibodies).

Programmable ultrasound-mediated swarms manipulation of bacteria-red blood cell microrobots for tumor-specific thrombosis and robust photothermal therapy.

Despite the excellent advantages of biomicrorobots, such as autonomous navigation and targeting actuation, effective penetration and retention to deep lesion sites for effective therapy remains a longstanding challenge. Here, we present dual-engine cell microrobots, which we refer to as PR-robots, created by conjugating photosynthetic bacteria (PSB) with red blood cells (RBCs). The robots penetrate the tumor interior in swarms through combined hypoxic traction and ultrasound actuation (UA).

Idiopathic non-cirrhotic portal hypertension: A case report.

Rationale: Idiopathic noncirrhotic portal hypertension (INCPH) is a rare liver disorder with elevated portal pressure without cirrhosis, making diagnosis challenging. This case report presents a 46-year-old Chinese male with INCPH, highlighting the crucial role of liver biopsy.

Patient Concerns: A 46-year-old male presented with persistent fatigue that lasted for 2 months and significantly worsened over the last 3 days.