Membrane contact sites between chloroplasts and the pathogen interface underpin plant focal immune responses.

Communication between cellular organelles is essential for mounting effective innate immune responses. The transport of organelles to pathogen penetration sites and their assembly around the host membrane, which delineates the plant-pathogen interface, are well-documented. However, whether organelles associate with these specialized interfaces, and the extent to which this process contributes to immunity, remain unknown.

[Research on hybrid brain-computer interface based on imperceptible visual and auditory stimulation responses].

In recent years, hybrid brain-computer interfaces (BCIs) have gained significant attention due to their demonstrated advantages in increasing the number of targets and enhancing robustness of the systems. However, Existing studies usually construct BCI systems using intense auditory stimulation and strong central visual stimulation, which lead to poor user experience and indicate a need for improving system comfort. Studies have proved that the use of peripheral visual stimulation and lower intensity of auditory stimulation can effectively boost the user's comfort.

Effectiveness of AI-assisted medical education for Chinese undergraduate medical students: a meta-analysis.

Background: In recent years, artificial intelligence (AI) has been adopted as an innovative teaching method. However, no studies have comprehensively evaluated the effectiveness of AI in the context of medical education in China. This study aimed to assess the effectiveness of AI-assisted teaching methods versus traditional teaching methods regarding their impact on medical education outcomes in China.

The Effectiveness of Nurse-Led Multidimensional Digital Cardiac Rehabilitation in Patients With Unstable Angina Undergoing Percutaneous Coronary Intervention: Emulated Target Trial.

Background: Cardiac rehabilitation (CR) interventions for patients with coronary heart disease are increasingly adopted. However, research on the integration of digital health technologies into CR for patients with unstable angina (UA) undergoing percutaneous coronary intervention (PCI) remains limited.

Objective: This study assessed the effectiveness of a multidimensional digital CR program for patients with UA undergoing PCI.

ProMethylNet: Protein Methylation Site Prediction Based on Multimodal Feature Fusion and Deep Learning.

Protein methylation is a fundamental post-translational modification (PTM) that plays critical roles in gene regulation, protein interactions, and cellular signaling pathways. However, traditional experimental approaches for identifying methylation sites are time-consuming, labor-intensive, and limited in throughput. Therefore, there is an urgent need for computational methods that efficiently and accurately predict methylation sites.

Origin-dependent metabolic variations: How Atractylodes macrocephalae Rhizoma extract's chemical diversity leads to stage-specific changes in simulated digestion.

Atractylodes macrocephalae Rhizoma (AMR), a traditional Chinese medicine, is extensively utilized in clinical practice for its pharmacological properties, including anti-inflammatory, anti-tumor, and gastrointestinal regulatory effects. Nonetheless, the intricate nature of traditional Chinese medicine extracts has resulted in few studies into the effects of compositional variations in Atractylodes macrocephalae Rhizoma extracts (AMRE) from diverse sources on gastrointestinal metabolic processes. This study developed an integrated in vitro and in vivo compound analysis strategy utilizing Ultrahigh-performance liquid chromatography Quadrupole-Orbitrap tandem mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) and the Simulator of Human Intestinal Microbial Ecosystem (SHIME) to examine the metabolic alterations caused by variations in the chemical constituents of AMRE from diverse sources.

Stiffness-Tunable Neurotentacles for Minimally Invasive Implantation and Long-Term Neural Activity Recordings.

Flexible microelectrodes are ideal for chronic neural recordings; however, their low bending strength poses challenges during probe insertion. Here, a stiffness-tunable polyimide probe, termed Neurotentacle, is proposed for deep brain implantation. Its tunability is enabled by embedded microchannels with controllable liquid pressure.

A New Cobalt-Based Metal-Organic Framework for Robust Electrocatalytic Oxygen Reduction at 200 mA cm.

Metal-organic frameworks (MOFs) have been widely exploited as promising heterogeneous catalyst, but it is still challenging for them to own the robustness of electrocatalysis at the current density of over 100 mA cm. Herein, one Co-N-based MOF is reported, featuring strong chelation interaction of metal/linker and strong interlayer π…π stacking, with to own extraordinary stability for alkaline ORR with almost unity of HO selectivity. It can robustly drive the ORR at 200 mA cm for 130 h, and produce HO with mass activity (7540 A·g ) and TOF value (40.

Epalrestat, an aldose reductase inhibitor, mitigates doxorubicin-induced cardiotoxicity by inhibiting glycometabolism.

Background: Doxorubicin (DOX) is a powerful chemotherapeutic drug with limited clinical effectiveness due to the risk of cardiotoxicity and glycometabolic disorders at high dosages. Epalrestat (EPS), a potent AKR1B1 inhibitor, has been extensively documented for its remarkable efficacy in treating glycometabolic disorders. We aimed to investigate the protective effects of EPS against DOX-induced cardiotoxicity and elucidate the underlying mechanisms.

Engineering Graphene Nanoribbons via Periodically Embedding Oxygen Atoms.

Heteroatom doping is an important method for engineering graphene nanoribbons (GNRs) because of its ability to modify electronic properties by introducing extra electrons or vacancies. However, precisely integrating oxygen atoms into the lattice of GNRs is unexplored, and the resulting electronic properties remain elusive. Here, we achieve the precise embedding of oxygen atoms into the lattice of GNRs via in situ formation of pyrans, synthesizing two types of oxygen-doped GNRs (O-doped chevron-GNR and O-doped chiral (2,1)-GNR).

Defending Against the Homodyne Detector-Blinding Attack on Continuous-Variable Quantum Key Distribution Using an Adjustable Optical Attenuator.

A homodyne detector, which is also a common element in current telecommunication, is a core component of continuous-variable quantum key distribution (CV-QKD) since it is considered the simplest setup for the distinguishing of coherent states with minimum error. However, the theoretical security of CV-QKD is based on the assumption that the responses of the homodyne detector are always linear with respect to the input, which is impossible in practice. In the real world, a homodyne detector has a finite linear domain, so the linearity assumption is broken when the input is too large.

A longitudinal EEG dataset of event-related potential.

Electroencephalogram (EEG)-based biometric emerges as a promising authentication method, offering a novel insight into the future security systems. However, its long-term stability and inter-individual variability necessitate further exploration. This paper presents an event-related potential (ERP) dataset acquired through EEG recordings under rapid serial visual presentation stimulation.

Pyrrolidinium Fullerenes as YTHDF1 Inhibitors for Targeted Tumor Therapy.

Cancer remains a leading cause of global morbidity and mortality, necessitating the development of novel targeted therapies. This study explores the therapeutic potential of pyrrolidinium fullerenes as YTH N-methyladenosine RNA binding protein 1 (YTHDF1) inhibitors for cancer treatment. A series of functionalized pyrrolidinium fullerenes is synthesized and characterized, including C-(N,N-dimethyl-pyrrolidinium iodide) (NDMPFI), C-(N-methyl-N-benzyl-pyrrolidinium iodide) (NMBPFI), and C-(N-methyl-N-hydroxyethyl-pyrrolidinium iodide) (NMHPFI).

Application of a self-made liver suspension device in 3D laparoscopic non-anatomical resection of liver segment VI and VII tumors.

Objective: To evaluate the efficacy of a novel self-designed liver suspension device in three-dimensional (3D) laparoscopic non-anatomical (NAR) resection for tumors in hepatic segments VI and VII.

Methods: Clinical records of 79 patients undergoing NAR resection of hepatic segments VI and VII at the Second Hospital of Hebei Medical University (June 2016-December 2021) were retrospectively reviewed. Patients were stratified into the Suspension Device Laparoscopic Group (SDLG), utilizing the self-designed suspension device for 3D-guided resection, and the Conventional Laparoscopic Group (CLG).

Boosting mRNA cancer vaccine efficacy via targeting on macrophages in lymph nodes.

mRNA cancer vaccines show great promise for tumor therapy, but the therapeutic efficacy is limited. Metabolites play critical roles in immunomodulation. However, their role in mRNA cancer vaccines remains poorly understood.

Efficacy and safety analyses of bevacizumab in neoadjuvant chemotherapy for ovarian cancer: a systematic review and meta-analysis.

Objective: To assess the efficacy and safety of bevacizumab in neoadjuvant chemotherapy for ovarian cancer through systematic evaluation and meta-analysis.

Methods: Online databases, such as PubMed, Embase, and the Cochrane Library, were searched for relevant articles on the treatment of ovarian cancer patients with interval debulking surgery after neoadjuvant chemotherapy in combination with the bevacizumab regimen using the keywords "Ovarian Neoplasms," "Bevacizumab," "Monoclonal antibodies against vascular endothelial growth factor," "Avastin," and "Neoadjuvant Therapy." A meta-analysis of the screened literature, which included randomized controlled trials and cohort studies, was then performed using Stata 15.

Discovery and identification of novel color compound formed from EGCG upon heating: Integrated multispectral, sensory evaluation, metabolomics, and nuclear magnetic resonance.

(-)-Epigallocatechin gallate (EGCG) is the major catechin in tea. However, its transformation mechanisms into sensory-active compounds during tea roasting remain unclear. LC-MS-based metabolomics and multispectral analysis discovered a variety of novel color compounds (NCCs) formed from EGCG during high-temperature heating, and these compounds were positively correlated with color indexes.

Mechanism of Huoshan large-leaf yellow tea in treatment of diabetic cognitive dysfunction based on bioinformatics and network pharmacology.

Background: Diabetic cognitive dysfunction (DCD) is a prevalent complication of diabetes, characterized by progressive cognitive decline. Current pharmacological interventions have limited therapeutic efficacy in managing this condition. Huoshan large - leaf yellow tea (HLYT), derived from Camellia sinensis leaves, has demonstrated dual pharmacological properties in both glycemic regulation and cognitive enhancement, suggesting its potential as a preventive and therapeutic agent for DCD.

Acoustic Enhancement Performance of Hierarchical ZSM-5 Zeolites with Different Si/Al Ratios.

Size restrictions pose increasing challenges to the acoustic performance of microspeakers in portable devices as the size of such devices, and thus the back volume of microspeakers, continues to shrink. Filling the back volume with porous materials, such as zeolites, has been proved to be an effective strategy for improving acoustic performance. In this work, hierarchically structured ZSM-5 zeolites with abundant mesopores were synthesized via the traditional hydrothermal method by adjusting the SiO/AlO ratios (SAR), and their pore structures and morphologies were systematically investigated.

Pinosylvin Inhibits Inflammatory and Osteoclastogenesis via NLRP3 Inflammasome.

Pro-inflammatory cytokines such as TNF, IL-1, and IL-6 trigger aberrant osteoclastogenesis and result in massive bone loss. During RANKL-induced osteoclastogenesis, pyroptosis of macrophages/preosteoclasts acts as a pivotal mechanism for IL-1β release, thereby promoting osteoclast maturation and bone resorption. In the current study, it is observed that Pinosylvin (PIN), a compound extracted from European red pine, selectively inhibits LPS- and RANKL-induced release of IL-1β effectively reducing osteoclastogenesis.

Deciphering a novel polysaccharide from Keemun black tea: structural elucidation and anti-colorectal cancer exploration.

Background: Keemun black tea (KBT) is one of the three major black teas in the world, which is famous for unique Keemun aroma and various health-promoting benefits. However, the structural characteristics and anti-tumor effects of the principal active polysaccharides in KBT have not been explored.

Results: In the present study, a novel homogeneous polysaccharide (KBPW1) with high anti-tumor activity was isolated from KBT by an activity-oriented sequential isolation procedure.

Analysis of Lymph Node Metastasis and Risk Factors in 424 Patients with Low-Grade Endometrioid Endometrial Carcinomas.

Objectives: The objective of this study was to explore the lymph node metastasis (LNM) and related risk factors of low-grade endometrioid endometrial carcinomas (EECs) and analyse the efficacy of related risk factors in predicting LNM.

Design: Data from 424 patients with low-grade EEC treated between January 2019 and June 2024 were retrospectively analysed, according to the International Federation of Gynecology and Obstetrics (FIGO) 2009.

Methods: Univariate and multivariate logistic regression analyses were used to examine the factors associated with LNM.

Effects of Lead Exposure on 1573 Male Workers' Sex Hormones in China.

Lead (Pb) is recognized as an environmental pollutant with male reproductive toxicity, but its effects on sex hormones remain unclear. This study investigated the relationship between male blood lead levels (BLLs) and the sex hormones of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and prolactin (PRL), as well as testosterone (T), estrogen (E2), and progesterone (PROG). Observational and experimental data from 1573 Pb-exposed workers (712 had also been surveyed in the previous year) and 35 Pb-poisoned patients (before and after Pb chelation therapy) were analyzed.