Effect of Periplaneta americana Extract on Corneal Fibrosis and Epithelial Healing After Rabbit Lamellar Keratectomy.

Purpose: To evaluate Periplaneta americana extract (PAE) effects on corneal epithelial healing and fibrosis after superficial lamellar keratectomy (SLK) in rabbits.

Methods: SLK was performed on the right eyes of 48 New Zealand White rabbits, randomized into three treatment groups (n = 16/group): normal saline (NS), Tobradex eye drops (TE), and PAE group. Corneal opacity and epithelial defect area were quantified using slit-lamp imaging at postoperative days 3, 7, 14, and 28 (D3, D7, D14, and D28) and scored via the grading system.

Research trends and topics on sepsis immunosuppression: a bibliometric and visual analysis of global research from 2004 to 2024.

Background: Sepsis, a condition characterized by critical organ impairment resulting from dysregulated response to infection, remains a leading cause of mortality globally. Immunosuppression significantly dictates the clinical trajectory and ultimate prognosis in sepsis. Research on sepsis-related immunosuppression has gained substantial attention in recent years.

The Application of iPSCs in Tumour Immunotherapy.

Background: Tumour immunotherapy holds great promise as a treatment for cancer, which ranks as the second highest cause of mortality worldwide. This therapeutic approach can be broadly categorized into two main types: active immunotherapy and passive or adoptive immunotherapy. Active immunotherapy, such as cancer vaccines, stimulates the patients' immune system to target tumour cells.

Multi-specialty Physician Preferences and Perceptions in Treatment and Management of Polycystic Ovary Syndrome in China: Survey and Discrete Choice Experiment.

Introduction: Polycystic ovary syndrome (PCOS) is one of the most common gynecological endocrine diseases in reproductive-aged women. Given the multifaceted nature of PCOS and the involvement of multiple disciplines, the diagnosis and treatment of PCOS vary among different specialties. Our study aimed to elicit preferences of physicians from multidisciplinary specialties for PCOS treatment and their perceptions of PCOS management to enhance the understanding of clinical practice in China.

Risk factors for hrHPV infection with cervical lesions patient in Anhui Province of China from 2021 to 2024: A retrospective study.

The association between high-risk HPV (hrHPV) infection and cervical cancer or precancerous lesions has been extensively established, highlighting the importance of preventing hrHPV infection in cervical lesions patients. This study aims to investigate the risk factors associated with hrHPV infection in patients with cervical lesions and develop a nomogram model for predicting the risk of infection. The study involved 154 patients with cervical lesions, all of whom underwent human papillomavirus (HPV) gene typing.

TCA cycle mode switch determines the fate of pirtobrutinib-tolerant persister cells in mantle cell lymphoma.

Bruton's tyrosine kinase inhibitors (BTKi) and cell therapy have successfully been used to treat mantle cell lymphoma (MCL); however, therapy resistance inevitably emerges. Cancer cells can progressively develop stable resistance by traversing through a transient drug-tolerant persister (DTP) state. The mechanisms enabling DTP cells to reversibly adapt to therapies and evolve to acquire heterogeneity remain poorly understood, and characterizing DTP cells in MCL continues to pose a challenge for clinic translation.

Synthesis of indoleacetic acid modified cellulose nanocrystals and their enhanced effect on the physical properties of pectin film.

Cellulose nanocrystals (CNCs) is extensively applied in bio-based biodegradable materials due to its unique properties. In this paper, an efficient oxidation method was proposed for the preparation of CNCs at mild reaction conditions (room temperature, 3 h) using a sodium bisulfite (NaHSO) activated potassium permanganate (KMnO) system. Subsequently, indoleacetic acid (IAA) modified CNCs (CNCI) were prepared by reacting CNCs with IAA, and the physical and chemical properties of CNCI were investigated by scanning electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analyser (TGA) and X-ray photoelectron spectroscopy (XPS).

Induction of abscopal effect combining H101 oncolytic virotherapy with tislelizumab in a patient with advanced hepatocellular carcinoma: a case report.

We reported a case of advanced hepatocellular carcinoma (HCC) in a patient who relapsed after first-line chemoimmunotherapy. Remarkably, the combination of H101 oncolytic virotherapy and tislelizumab successfully induced an abscopal effect. Following this treatment, the patient achieved a 24-month survival period, accompanied by complete regression of distant metastatic lesions.

Establishment of an infectious clone of the porcine transmissible gastroenteritis virus and a study on the location and function of accessory protein 3.

Introduction: Transmissible gastroenteritis virus (TGEV), as the causative agent of TGE, causes huge economic losses in the pig industry worldwide.

Methods: In this study, we successfully constructed a reverse genetic system for the TGEV TH-98 strain and rescued the recombinant virus rTH-98-Δ3-COE-HA based on this system.

Results: The results showed that ORF3 is a non-structural protein and does not exist in mature virions.

The influence of post-processing methods and frequency bands on rs-fMRI: An example of electroacupuncture at Zusanli (ST36).

Objective: Electroacupuncture at Zusanli (ST36) exerted an effect on brain function by regulating spontaneous brain activity. This study aims to investigate the influence of different post-processing methods of amplitude of low-frequency fluctuation (ALFF) and different frequency band to measure brain low-frequency oscillations (LFOs) after electroacupuncture at ST36.

Methods: Twenty-eight healthy subjects were recruited and received true electroacupuncture (EA) and sham electroacupuncture (SA) at ST36 respectively.

Revolutionizing periodontitis treatment: The promise of GelMA hydrogel.

Periodontitis is a common oral inflammatory disease that causes persistent damage to periodontal soft and hard tissues. The current clinical treatments for periodontitis all have varying degrees of defects. As a promising biomaterial, gelatine methacryloyl (GelMA) exhibits favorable biocompatibility and modifiable physicochemical properties, demonstrating considerable potential for applications in periodontal disease therapy.

S100a8 inhibitor Paquinimod attenuates coal dust-induced pulmonary fibrosis via blocking neutrophils-S100a8-Tlr4-macrophages signaling axis.

Prolonged inhalation of environmental dust leads to the onset of life-threatening pulmonary fibrosis. Identifying therapeutic targets and drugs for this disease is of critical urgency. However, traditional drug discovery often overlooks cell communication signals.

Modified Citrus Pectin Alleviates Diabetic Kidney Disease by Suppressing Pyroptosis in Renal Tubular Epithelial Cells via the Cx43-ERK1/2 Signaling Pathway.

Modified citrus pectin (MCP) exhibits antioxidant properties, while persistent oxidative stress is one of the key mechanisms contributing to the progression of diabetic kidney disease (DKD). However, its role and underlying mechanisms in the progression of DKD remain inadequately understood. The objective is to clarify the functions of MCP in the regulation of renal tubular epithelial cell injury and to substantiate the underlying mechanisms.

Evaluation of the effects of melanin bleaching in different steps of immunohistochemistry on an automated platform.

Melanin is a granular brown-black pigment unevenly distributed in tissues. Severe melanin deposition can obscure cellular morphological features and tissue structures, hindering the evaluation of melanocytic lesion. This condition is particularly prone to interfering with the evaluation of immunohistochemistry (IHC), thereby affecting diagnostic accuracy.

Unraveling the cross-talk between a highly virulent PEDV strain and the host via single-cell transcriptomic analysis.

Porcine epidemic diarrhea virus (PEDV) causes severe intestinal damage and high mortality in neonatal piglets. The continuous emergence of new strains has brought new challenges to prevention and control. In this study, we isolated and characterized a prevalent PEDV virulent strain and analyzed 19,612 jejunal cells from PEDV-infected and control piglets using single-cell sequencing, revealing significant changes in cellular composition, gene expression, and intercellular communication.

Construction of emergency competency models for nurses in emerging high-consequence infectious disease outbreaks: Behavioural event interview approach.

Background: Emerging high-consequence infectious disease outbreaks (EHCIDs) have significantly impacted healthcare systems worldwide. Nurses specialising in outbreak response nurses play an important role in the prevention and control of EHCIDs. However, the specific competencies required for these nurses remain undefined.

Selective radical-type perfluoro-tert-butylation of unsaturated compounds with a stable and scalable reagent.

Despite the promising potential of the perfluoro-tert-butyl group in diverse fields such as magnetic resonance imaging, material science and drug design, incorporating this group into organic molecules is still a formidable task, primarily due to its bulky structure and unique fluorine effect. Herein, we describe a stable and scalable reagent for radical-type perfluoro-tert-butylation, which is synthesized in large scale from commercial perfluoro-tert-butanol and a designed benzothiazole hypervalent iodonium salt. Highly E-selective photo-driven C(sp)-H functionalization of styrene derivatives is achieved in a triplet-triplet energy transfer halted manner, while thermally disfavored Z-products are also accessible by removing the energy antagonist.

Interpretable machine learning models to predict decline in intrinsic capacity among older adults in China: a prospective cohort study.

Background: Monitoring intrinsic capacity and implementing appropriate interventions can support healthy aging. There are, though, few tools available for predicting decline in intrinsic capacity among older adults. This study aimed to develop and validate an interpretable machine learning model designed to identify populations at elevated risk of a decline in intrinsic capacity.

Development and evaluation of immunogenicity and protective efficacy of two recombinant attenuated newcastle disease viruses expressing the VP2 protein of infectious bursal disease virus.

Newcastle disease (ND) and Infectious bursal disease (IBD) are highly contagious and economically significant viral diseases affecting poultry worldwide. Despite widespread vaccination, current vaccines often require multiple doses and provide limited protection against emerging strains. This study developed and evaluated bivalent vaccines based on recombinant Newcastle disease virus (NDV) expressing the VP2 protein of a novel variant Infectious bursal disease virus (IBDV), SHG19 strain.

Evaluating the MS-HLJ strain as a novel inactivated vaccine candidate: Long-term immune protection against Mycoplasma synoviae in poultry.

Mycoplasma synoviae (MS) is highly prevalent in chicken flocks and causes significant economic losses in the poultry industry. Vaccination is an effective measure for preventing and controlling MS infection in chickens, making it crucial to isolate, identify, and analyze the immunogenicity of MS to develop vaccines against mycoplasmosis in poultry. In this study, the MS-HLJ strain was isolated from the joint tissue fluid of a suspected case of MS infection in chickens, and its pathogenicity in specific-pathogen-free chickens was investigated.

Ivarmacitinib for Moderate to Severe Atopic Dermatitis in Adults and Adolescents: A Phase 3 Randomized Clinical Trial.

Importance: Ivarmacitinib, a selective oral Janus kinase 1 (JAK1) inhibitor, has demonstrated efficacy for treating adults with moderate to severe atopic dermatitis (AD) in a phase 2 trial.

Objective: To evaluate the efficacy and adverse events of ivarmacitinib in adolescents and adults with moderate to severe AD.

Design, Setting, And Participants: This multicenter, double-blind, placebo-controlled phase 3 randomized clinical trial included patients aged 12 to 75 years with moderate to severe AD.

Expressing Porcine Trefoil Factor 3 and Epidermal Growth Factor: A Novel Approach for Superior Mucosal Repair.

Trefoil factor 3 (TFF3) and epidermal growth factor (EGF) exert a promotive effect on the functions of intestinal epithelial cells and offer protection to the intestinal mucosa. can ameliorate intestinal mucosal damage. In this study, pPG-pTFF3/, pPG-pEGF/, and pPG-pTE/ were constructed to express porcine TFF3, EGF, and a fusion protein (pTE).

SLC26A4 C.317C > A Variant: Functional Analysis and Patient-Derived Induced Pluripotent Stem Line Development.

Background: SLC26A4 is the second most common cause of hereditary hearing loss worldwide. This gene predominantly harbors pathogenic variants, including splice, nonsense, and missense. Although missense variants are relatively common, their specific effects on protein function remain unclear.

Potential target within the tumor microenvironment - MT1-MMP.

Matrix metalloproteinases are integral to the modification of the tumor microenvironment and facilitate tumor progression by degrading the extracellular matrix, releasing cytokines, and influencing the recruitment of immune cells. Among the matrix metalloproteinases, membrane-type matrix metalloproteinase 1 (MT1-MMP/MMP14) is the first identified membrane-type MMP and acts as an essential proteolytic enzyme that enables tumor infiltration and metastatic progression. Given the pivotal role of MT1-MMP in tumor progression and the correlation between its overexpression in tumors and unfavorable prognoses across multiple cancer types, a comprehensive understanding of the potential functional mechanisms of MT1-MMP is essential.