Deep Learning Based Multiomics Model for Risk Stratification of Postoperative Distant Metastasis in Colorectal Cancer.

Rationale And Objectives: To develop deep learning-based multiomics models for predicting postoperative distant metastasis (DM) and evaluating survival prognosis in colorectal cancer (CRC) patients.

Materials And Methods: This retrospective study included 521 CRC patients who underwent curative surgery at two centers. Preoperative CT and postoperative hematoxylin-eosin (HE) stained slides were collected.

Development and validation of models based on clinical and CT features: multivariate analysis for predicting vascular invasion in non-small cell lung cancer.

Background: Lymphovascular invasion (LVI) is a high-risk pathological marker for the evaluation of metastasis and prognosis of non-small cell lung cancer (NSCLC). Preoperative computed tomography (CT) prediction of vascular invasion in NSCLC is essential for clinical identification of high-risk patients and development of treatment strategies. This study aimed to develop and validate a model for predicting LVI in NSCLC based on clinical and CT features.

A Glucose Oxidase-Nanozyme Cascade System with Adjustable Interenzyme Spacing for Glucose Analysis.

Here, glucose oxidase (GOD) is connected to FeO nanozyme by using epoxy cross-linkers as a spacer arm to construct a GOD-nanozyme artificial enzyme cascade system that is easy to separate magnetically. The dual-enzyme system can regulate the distance between GOD and nanozyme by changing the chain length of the cross-linker. The optimal interenzyme spacing of 2 nm has led to a significant increase in the cascade activity of the dual-enzyme system to 1400 U/mg, which is 2.

Microneedle-mediated delivery of hydroxypropyl-β-cyclodextrin-encapsulated Angelica sinensis (Danggui) essential oil for acne treatment: efficacy and mechanisms.

Acne is a multifactorial dermatological condition driven by Propionibacterium acnes (P. acnes) infection, inflammation, and sebum dysregulation. While essential oils, such as Angelica sinensis (Danggui) essential oil (AEO), exhibit antimicrobial and anti-inflammatory properties, their clinical application is hindered by poor solubility, stability, and skin penetration.

[Study on the mechanism of moxibustion in improving autophagy of intestinal epithelial cells in rats with diarrhea-predominant irritable bowel syndrome based on PI3K/AKT/mTOR signaling pathway].

Objectives: To observe the effect of moxibustion on visceral hypersensitivity and the phosphatidylinositol-3 kinase (PI3K) /protein kinase B (AKT) /mammalian target of rapamycin (mTOR) signaling pathway in rats with diarrhea-predominant irritable bowel syndrome (IBS-D), so as to explore its mechanisms underlying amelioration of pain reaction of IBS-D by regulating the autophagy level of intestinal epithelial cells and protecting the intestinal mucosal barrier.

Methods: Forty male SD rats were randomly divided into control, model, moxibustion and moxibustion+LY294002 groups, with 10 rats in each group. The IBS-D model was established by acetic acid enema + chronic binding method.

A novel MRI-based habitat analysis and deep learning for predicting perineural invasion in prostate cancer: a two-center study.

Background: To explore the efficacy of a deep learning (DL) model in predicting perineural invasion (PNI) in prostate cancer (PCa) by conducting multiparametric MRI (mpMRI)-based tumor heterogeneity analysis.

Methods: This retrospective study included 397 patients with PCa from two medical centers. The patients were divided into training, internal validation (in-vad), and independent external validation (ex-vad) cohorts (n = 173, 74, and 150, respectively).

Genome-wide analysis of the AarPDR gene family in Artemisia argyi and its potential role in regulating terpenoids transport.

Background: The pleiotropic drug resistance (PDR) proteins, which are full-sized ABCG transporters belonging to the ATP-binding cassette (ABC) family, play an indispensable role in regulating various physiological and developmental processes of plants. However, the PDR transporters and their functional roles in Artemisia argyi, a significant traditional medicinal plant in Asia, remain poorly understood.

Results: In this study, a total of 68 PDR genes, distributed across 17 chromosomes, were identified in the genome of Artemisia argyi and classified into five distinct subgroups based on phylogenetic analysis and domain types.

G protein-coupled estrogen receptor promotes human extravillous trophoblast invasion via YAP-Snail-mediated CYR61 expression.

Preeclampsia (PE) is a pregnancy-related disorder characterized by impaired extravillous trophoblast (EVT) invasion, leading to defective placental development. The G protein-coupled estrogen receptor (GPER) is expressed in human EVT cells and has been implicated in promoting cell invasion. Cysteine-rich angiogenic inducer 61 (CYR61), also known as CCN1, is a matricellular protein that facilitates cell migration and invasion; however, whether GPER activation regulates CYR61 expression in EVT cells remains undefined.

Acetaminophen overdose suppresses human extravillous trophoblast invasion by downregulating SP1-mediated MMP2 expression.

In Brief: Acetaminophen (APAP) overdose impairs extravillous trophoblast (EVT) cell invasion by inhibiting AKT signaling, leading to downregulation of SP1 and reduced MMP2 expression. These findings highlight a potential mechanism by which excessive APAP use may compromise early placental development and pregnancy outcomes.

Abstract: Extravillous trophoblast (EVT) cell invasion is crucial for the establishment of proper maternal-fetal circulation and successful pregnancy outcomes.

Smart responsive biomaterials for spatiotemporal modulation of functional tissue repair.

The biological characteristics and microenvironmental changes at each stage of tissue repair are intricately linked, maintaining a dynamic spatiotemporal equilibrium. Monotherapies often fail to effectively address complex microenvironmental disturbances, but combining multiple factors (e.g.

Ultrasound-responsive piezoelectric analgesic microspheres alleviate osteoarthritis pain.

Osteoarthritis (OA) is one of the most common causes of chronic pain. However, current OA pain therapies often fail to achieve satisfactory outcomes. To effectively alleviate OA pain, ultrasound-responsive piezoelectric analgesic microspheres, constructed using ultrasound and piezoelectric nanomaterials, were developed.

Exosomes derived from platelet-rich plasma alleviate synovial inflammation by enhancing synovial lymphatic function.

Background: Synovial inflammation is a pivotal factor in the pathogenesis of osteoarthritis (OA). Platelet-rich plasma-derived Exosomes (PRP-Exos), known for their low immunogenicity, have demonstrated efficacy in modulating chondrocyte function. However, the specific effects and mechanisms of PRP-Exos in synovial inflammation remain unclear.

[Effect of moxibustion on small intestinal mucosal immune barrier in rats with diarrhea-predominant irritable bowel syndrome].

Objective: To observe the effect of moxibustion on small intestinal mucosal immune barrier in rats with diarrhea-predominant irritable bowel syndrome (IBS-D) and explore its underlying mechanisms.

Methods: Of 38 newborn rats from 4 healthy SPF pregnant rats, 12 neonatal rats were randomly selected in a normal group. IBS-D model was prepared by the combined measures for the rest rats, including neonatal maternal separation, acetic acid enema and chronic restraint stress.

Patterns and trends of medication use among patients with bipolar disorder in Northeast China: A study from a large psychiatric center (2013-2022).

Background: The medication patterns and trends among bipolar disorder patients in mainland China remain unclear. This study, as the first attempt in northeast China, is designed to clarify the medication patterns and elucidate medication trends of bipolar disorder patients from 2013 to 2022.

Methods: The study included 1812 bipolar disorder patients from a large psychiatric center who received treatment from 2013 to 2022.

Acousto-Electric Conversion Fiber Networks via Regional Activation of Schwann Cell-Derived Exosomes for Neurogenic Bone Regeneration.

Neurogenic bone regeneration is essential for the effective restoration of bone tissue functionality, with exosomes derived from Schwann cells regionalized in bone injury tissue playing a crucial role in this process. However, precisely regulating the secretion of Schwann cells localized in bone injury tissue to enhance neurogenic bone regeneration remains a considerable challenge. In this study, an injectable, ultrasound-responsive piezoelectric conductive short fiber network (US@SFG) was innovatively developed using uniform short fiber homogenization techniques and multifunctional chemical modifications, enabling precise acoustic-electrical conversion that regionally activated the secretion of miRNAs from Schwann cell-derived exosomes, thereby promoting neurogenic bone regeneration.

Unexpected Clinically Significant Drug-Drug Interaction between Tacrolimus and Metronidazole in the Early Period after Renal Transplantation: A Literature Review.

Introduction: Drug interactions necessitate careful consideration in clinical practice. It is imperative for clinicians and pharmacists to monitor drug exposure and the co-administration of medications promptly in order to avert adverse outcomes and achieve optimal efficacy.

Objectives: The prevalence of oral lesions varies from 28% to 60% in the short term after renal transplantation.

Crosstalk between mitochondrial quality control and novel programmed cell death in pulmonary diseases.

Dysfunctional mitochondrial quality control (MQC) and dysregulated programmed cell death (PCD) are increasingly recognized as key drivers of pulmonary diseases. This review explores the intricate crosstalk between MQC mechanisms, encompassing mitochondrial biogenesis, dynamics, mitophagy, and mitocytosis, and novel PCD pathways such as pyroptosis, ferroptosis, necroptosis, PANoptosis, cuproptosis, and disulfidptosis. We highlight how mitochondrial dysfunction triggers PCD and how PCD exacerbates mitochondrial damage, creating a vicious cycle that amplifies lung injury and inflammation.

Adsorption of weak polar zearalenone mycotoxins from water environment by tourmaline mineral powder of spontaneous electrical polarization properties.

Zearalenone (ZEN), a weakly polar mycotoxin that is widespread in the water environment, is difficult to remove and poses potential hazards to animal, human, and environmental health. In this study, the adsorption performance of weakly polar ZEN in the water environment was investigated in detail, focusing on the enhancement effect of spontaneous electrical polarization of tourmaline mineral powder on the adsorption process. The results showed that the tourmaline mineral powder exhibited satisfactory adsorption ability for ZEN mycotoxin from water environment, with a maximum theoretical adsorption capacity of 10.

Differential patterns of the relationship between exercise dose and mortality risk across severities of airflow limitation: a prospective cohort study with a 5-year follow-up period.

Objective: This study examines the dose-response relationship between physical activity (PA) and all-cause mortality across different severities of airflow limitation, identifying threshold effects that yield new insights into the PA-mortality association.

Design: A prospective cohort study with a 5-year follow-up (2018-2023), employing multivariate Cox models and penalized spline smoothing to assess non-linear associations.

Subjects/patients: A total of 2,975 individuals from a cohort categorized by airflow limitation severity (normal, GOLD 1-4).

The Application of Ultrasound-Induced Blood-Brain Barrier Opening in Neurology and Immunology.

The blood-brain barrier (BBB) acts as a stringent biological filter situated between the circulatory system and the brain's tissue, shielding the brain by preventing the entry of detrimental neurotoxic substances into the central nervous system (CNS). Nevertheless, the BBB also restricts the entry of most therapeutic drugs, resulting in only a few medications currently available for the clinical treatment of neurological diseases. Consequently, it is imperative to discover a secure and potent technique to temporarily disrupt the BBB for the purpose of directed medication administration.

CYR61 and CTGF mediate the stimulatory effect of amphiregulin on COX-2 expression in human granulosa-lutein cells.

Amphiregulin (AREG), an epidermal growth factor (EGF)-like ligand, is the predominant epidermal growth factor receptor (EGFR) ligand in human follicular fluid and mediates the effects of luteinizing hormone (LH) on ovarian function. In this study, we investigated whether AREG regulates the expression of cysteine-rich angiogenic inducer 61 (CYR61) and connective tissue growth factor (CTGF), two key matricellular proteins involved in ovarian function, and whether they mediate AREG-induced cyclooxygenase-2 (COX-2) expression. Using the human granulosa cell tumor-derived KGN cell line and primary human granulosa-lutein (hGL) cells, we demonstrated that AREG treatment upregulated CYR61 and CTGF protein levels in an EGFR-dependent manner.

Phthalide extract from stems and leaves of Ligusticum chuanxiong attenuates cerebral I/R injury via CaSR-mediated NLRP3 inflammasome suppression.

Ethnopharmacological Relevance: The stems and leaves of Ligusticum chuanxiong ("Miwu") have been historically documented in ancient texts such as "Shennong Bencao Jing Jizhu" for alleviating conditions like stroke and dizziness. However, the therapeutic potential of phthalide extract from the stems and leaves of Ligusticum chuanxiong (SLECX) needs further investigate.

Aim Of The Study: This study aimed to investigate the neuroprotective effects of SLECX against cerebral ischemia-reperfusion (I/R) injury and elucidate its mechanism of action.

Bupropion decreases plasma levels of asymmetric dimethylarginine and ameliorates renal injury by modulation of Ddah1, Oatp4c1, Oct2, and Mate1 in rats with adenine-induced chronic renal injury.

Objective: The objective of the study was to investigate whether bupropion (BUP) or its circulation metabolites could decrease plasma level of asymmetric dimethylarginine (ADMA) and ameliorate renal injury by modulation of Ddah1, Oatp4c1, Oct2, and Mate1 in rats with adenine-induced chronic renal injury.

Methods: The study initially determined the effect of BUP and its metabolites on cell viability and apoptosis in HK2 cells in the presence and absence of ADMA. Secondly, the study explored whether long-term administration of BUP could reduce the plasma level of ADMA and mitigate renal damage.

Effect of Electroacupuncture on Intestinal Mucosal Barrier in IBS-D Rats: Analysis Based on RNA-seq.

Objective: Currently, transcriptome-level investigations into the therapeutic mechanisms of electroacupuncture (EA) on intestinal mucosal barrier dysfunction in diarrhoea-predominant irritable bowel syndrome (IBS-D) models remain scarce. This study was designed to establish a comprehensive competing endogenous RNA (ceRNA) network through integrated RNA sequencing (RNA-seq) and bioinformatics analyses while elucidating the underlying mechanisms through which EA restores intestinal barrier integrity in IBS-D rats via modulation of the long non-coding RNA (lncRNA)-microRNA (miRNA) -messenger RNA (mRNA) regulatory network.

Methods: The IBS-D model was established by neonatal maternal separation (NMS), 4% acetic acid enema, and restrain stress (RS).

Exogenous Thiamine Regulates Multiple Plant Immunity-Associated Pathways to Inhibit MCMV Infection.

Thiamine, also known as vitamin B1, serves as an inducer that strengthens plants and elicits defense responses to enhance resistance against pathogens. Currently, the antiviral mechanism of thiamine remains unclear. Here, we used maize chlorotic mottle virus (MCMV) as a model to elucidate the antiviral mechanism of thiamine.