A Novel Self-Regulated, Non-Directional Magnetic Thermos-Brachytherapy I Seed Enhances Anticancer Efficacy by Rescuing Immune Escape.

Integrating mild hyperthermia (MH) with I brachytherapy holds potential for overcoming treatment resistance and improving anticancer efficacy. Here, magnetic nanoparticles (MNPs) with a suitable Curie temperature are constructed and incorporated with silver rods coated with I to form composite seeds. In vitro simulations and in vivo validations demonstrated their effective performance in radiation dose and temperature control.

[Study on the mechanism of moxibustion in improving autophagy of intestinal epithelial cells in rats with diarrhea-predominant irritable bowel syndrome based on PI3K/AKT/mTOR signaling pathway].

Objectives: To observe the effect of moxibustion on visceral hypersensitivity and the phosphatidylinositol-3 kinase (PI3K) /protein kinase B (AKT) /mammalian target of rapamycin (mTOR) signaling pathway in rats with diarrhea-predominant irritable bowel syndrome (IBS-D), so as to explore its mechanisms underlying amelioration of pain reaction of IBS-D by regulating the autophagy level of intestinal epithelial cells and protecting the intestinal mucosal barrier.

Methods: Forty male SD rats were randomly divided into control, model, moxibustion and moxibustion+LY294002 groups, with 10 rats in each group. The IBS-D model was established by acetic acid enema + chronic binding method.

Lewis Acid-Catalyzed Cyclization of -Hydroxyphenyl Propargylamines with Sodium Sulfinates: Syntheses of 3-Sulfonylbenzofurans.

A Lewis acid-catalyzed cyclization for the synthesis of 3-sulfonylated benzofurans using -hydroxyphenyl propargylamines and sodium sulfinate compounds has been developed. This reaction proceeds through: (i) acid-mediated in situ generation of -AQMs, (ii) sulfite ion-initiated sulfa-Michael addition, followed by (iii) Lewis acid-promoted annulation, enabling efficient construction of 3-sulfonylbenzofurans in generally good to excellent yields.

Yttrium nitrate activates the oxidative stress-mediated NF-κB pathway to induce testicular inflammatory response and reduce sperm quality in mice.

The rising environmental levels of yttrium have sparked concerns regarding its possible health hazards. Nevertheless, limited toxicological data are available to determine yttrium's toxicity and potential mechanisms on sperm. In our research, the action of oxidative stress and the NF-κB pathway on decreased sperm quality and testicular inflammatory reaction induced by yttrium exposure was analyzed.

[Identification of tissue distribution components and mechanism of antipyretic effect of famous classical formula Dayuanyin].

Based on the ultra performance liquid chromatography-quadrupole Exactive Orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS) technology, combined with related literature, databases, and reference material information, this study qualitatively analyzed the components of Dayuanyin in the tissue of rats after gavage and employed molecular docking technology to predict the rationality of the mechanism behind the antipyretic effect of the in vivo components in Dayuanyin. A total of 21, 26, 20, 21, 14, and 31 prototype components and 3, 16, 3, 7, 5, and 24 metabolites were identified from the heart, liver, spleen, lung, kidney, and hypothalamus of the rats, respectively, and the binding ability of key components and targets was further verified by molecular docking. The results showed that all components had good binding ability with targets.

RGS1 is an effective T-cell marker of acute myeloid leukemia remission after hematopoietic stem cell transplantation.

Disease relapse is a major cause of death in acute myeloid leukemia (AML) patients after allogeneic hematopoietic stem cell transplantation (allo-HSCT). The characterization of novel functional T-cell subtypes is critical for predicting clinical responses as well as developing strategies for immunotherapy in leukemia. We used single-cell RNA-sequencing to resolve the T cells' profiles of AML patients who had a relapse (RL) or reached complete remission (CR) after HSCT and addressed the characteristics of T cells at molecular level under HSCT scenario.

Assessing macroalgae composition in surface sediments of the Ma'an Archipelago seaweed field based on eDNA technology.

Macroalgae contribute to the seafloor sediments as detritus when they die or detach. Tracking the distribution of macroalgae is crucial to understanding their contribution to seafloor sediments. To assess species composition and distribution of macroalgae in sediments, we used environmental DNA (eDNA) technology to analyze surface sediments from four typical island waters, including Ma'an Archipelago Special Marine Protected Area, eas-tern Lyuhua (DLH), western Lyuhua (XLH), Mantou Mountain (MTS), and Mandui Mountain (MDS).

A Comparative Research of the Flavonoid Metabolites From Viscum coloratum in Normal and RA Rats by an Integrated Analytical Strategy.

Viscum coloratum (Kom.) Nakai has been demonstrated to be an effective treatment for rheumatoid arthritis (RA), but the pharmacodynamic substances are still unclear. In this study, a four-step strategy integrated nontargeted metabolomics, multivariate statistical analysis, and UNIFI software.

BPA-induced disruption of muscle development and larval metamorphosis in the mussel Mytilus coruscus.

Bisphenol A (BPA) has been found in aquatic environments worldwide, raising concerns about its potentially harmful effects, particularly during the early stages of organismal development. In this study, exposure to BPA significantly inhibited the development of Mytilus coruscus larvae and led to abnormal muscle development, characterized by a reduction in adductor muscle size and a decrease in the branching of velum retractor muscles. BPA exposure impaired larval metamorphosis, with reduced metamorphosis rates at 0.

Practical microenvironment classification in diffuse large B cell lymphoma using digital pathology.

Diffuse large B cell lymphoma (DLBCL) is a heterogeneous B cell neoplasm with variable clinical outcomes influenced by both tumor-derived and lymphoma microenvironment (LME) alterations. A recent transcriptomic study identifies four DLBCL subtypes based on LME characteristics: germinal center (GC)-like, mesenchymal (MS), inflammatory (IN), and depleted (DP). However, integrating this classification into clinical practice remains challenging.

Nickel-Catalyzed Hydrocarbamoylation of Alkenes with Isocyanates.

The hydrocarbamoylation of alkenes with isocyanates is a promising method for synthesizing amides. However, applying this strategy to more inert, simple alkenes, such as styrenes, α-olefins, and internal alkenes, poses significant challenges. Here, we report the first nickel-catalyzed hydrocarbamoylation of alkenes with isocyanates, facilitated by triethoxysilane to reduce nickelacycle intermediates.

An integrated analysis strategy for characterization of chromones and coumarins from Saposhnikovia divaricata (Turcz.) Schischk. by UHPLC-QTOF-MS.

This study aimed to employ a comprehensive data screening strategy to identify the chromones and coumarins present in Saposhnikovia divaricata (SD). Initially, the five-point mass defect filter (MDF) method was utilized to screen the respective three subclasses of chromones and coumarins for MS. In comparison to the traditional MDF method, the number of interference peaks was reduced from 3462 to 1053, representing a decrease of 69.

Engineering Electron Lifetime for High-Performance Heterostructured 1D CdS Photocatalyst.

The development of high-performance photocatalysts is essential for advancing sustainable hydrogen production. In the present work, an innovative approach to electron lifetime engineering aimed at enhancing the photocatalytic performance of 1D CdS nanowires by strategically incorporating Ni and titanium nitride (TiN) layers. It demonstrates the electron lifetime mechanism of 1D photocatalyst can be optimized through the introduction of uneven surface and heterojunction.

Six new compounds with neuroprotective activity from the leaves of Paeonia lactiflora Pall.

Twenty constituents, three new terpenes named Paeoniterpene A-C (1-3), and three new fatty glycosides named Paeoniglycoside A-C (4-6) were isolated from the leaves of Paeonia lactiflora Pall. The structures of compounds 1-6 were identified using extensive spectroscopic methods, such as NMR, MS, CD, IR, and UV. Compounds 4-6 were characterized through quantum chemical calculations and DP4 probability analysis, and ECD definitively confirmed their structures.

[Pharmacokinetics study of Dayuanyin in normal and febrile rats].

Based on the pharmacokinetics theory, this study investigated the pharmacokinetic characteristics of albiflorin, paeoniflorin, wogonoside, and wogonin in normal and febrile rats and summarized absorption and elimination rules of Dayuanyin in them to provide reference for further development and clinical application of Dayuanyin. Blood samples were taken from the fundus venous plexus of normal and model rats after intragastric administration of Dayuanyin at different time points. The concentration of each substance in blood was determined by ultra performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS/MS) technique at different time points.

Nicotinamide Phosphoribosyltransferase Acetylation Mediating Muscle Dysfunction Contributes to Sleep Apnoea in Obesity.

Background: Obstructive sleep apnoea (OSA) occurs frequently among individuals with obesity, which is attributed to upper airway muscle dysfunction. Muscle function is regulated by the dynamic balance of the nicotinamide adenine dinucleotide (NAD+) and its reduced form (NADH), which is controlled by the enzyme nicotinamide phosphoribosyltransferase (NAMPT). Elevated NAMPT levels have been found in individuals with obesity.

Chimeric Peptide Functionalized Immunostimulant to Orchestrate Photodynamic Immunotherapeutic Effect by PD-L1 Deglycosylation and CD47 Inhibition.

Breast cancer utilizes diverse immunosuppressive mechanisms to evade immune surveillance, thereby impairing immunotherapeutic effects. In this work, a chimeric peptide functionalized immunostimulant (designated as aGlyR) is fabricated to boost photodynamic immunotherapy through PD-L1 deglycosylation and CD47 inhibition. The photosensitizer protoporphyrin IX (PpIX) is conjugated to a PD-L1 deglycosylation peptide via a hydrophilic PEG linker, yielding the chimeric peptide Fmoc-K(PpIX)-PEG-GFTATPPAPDSPQEP.

Identification of the Novel HLA-B*40:01:82 Allele by Sanger Dideoxy Nucleotide Sequencing in a Chinese Individual.

The HLA-B*40:01:82 allele differs from HLA-B:40:01:01 by a single synonymous nucleotide in exon 4.

Per- and polyfluoroalkyl substances (PFASs) inhibit larval metamorphosis by impairing larval muscle degeneration in the mussel Mytilus coruscus.

Per- and polyfluoroalkyl substances (PFASs), including perfluorooctane sulfonate (PFOS) and its alternative 6:2 chlorinated polyfluoroalkyl ether sulfonate (F53B), are widely used in industries, leading to their presence in aquatic environments and potential adverse effects on marine organisms, particularly during early development. This study investigates the effects of PFOS and F53B on larval development and metamorphosis in Mytilus coruscus. Exposure to 4.

New cytotoxic compounds from (Kom.) Nakai.

Twenty-one compounds were isolated and identified from the ethanol extract of (Kom.) Nakai, including two new compounds ( and ), a new artificial product () and eighteen known compounds (). Compounds and were isolated from for the first time.

Acupuncture at Weizhong (BL40) attenuates acetic acid-induced overactive bladder in rats by regulating brain neural activity through the modulation of mast cells and tibial nerves.

Objective: The present study evaluated the effects of deep acupuncture at Weizhong acupoint (BL40) on bladder function and brain activity in a rat model of overactive bladder (OAB), and investigated the possible mechanisms around the acupuncture area that initiate the effects of acupuncture.

Methods: Adult female Sprague-Dawley rats were randomly divided into six groups, comprising a control group, model group, group treated with deep acupuncture at BL40, group treated with shallow acupuncture at BL40, group treated with acupuncture at non-acupoint next to BL40, and group treated with acupuncture at Xuanzhong (GB39). Urodynamic evaluation was used to observe the urination, and functional magnetic resonance imaging was used to observe the brain activation.

Diabetic ulcer with cutaneous squamous cell carcinoma: A case report.

Background: Chronic skin ulcers are a risk factor for the development of skin tumors. In patients with diabetes, chronic refractory ulcers may also contribute to higher susceptibility to skin tumors. Timely surgical removal of chronic and nonhealing diabetic foot ulcers can effectively prevent progression to squamous cell carcinoma.