Association between creatinine clearance and mortality in Chinese patients with osteoporotic fractures: a retrospective cohort study.

Background: Creatinine clearance (CCR) is a vital biomarker for evaluating renal function, indicating the efficiency of the kidneys in filtering blood waste. However, the link between CCR and mortality in hospitalized patients with osteoporotic fractures (OPFs) remains unclear. The increasing prevalence of OPFs in elderly populations, coupled with known complications of renal dysfunction, underscores the critical importance of understanding this relationship.

Association between CDSS score and 30-day mortality in nonAPL acute leukemia patients with intracranial hemorrhage: a cohort study.

Objectives: The aim of this study was to investigate the correlation between CDSS (Chinese DIC Scoring System) score and 30-day mortality in patients with intracranial hemorrhage (ICH) with nonacute promyelocytic leukemia (APL) acute leukemia.

Methods: This cohort study enrolled patients with non-APL acute leukemia complicated by ICH. The CDSS score was assessed in this patient population.

Comprehensive bioinformatic analysis identifies potential therapeutic drugs for CryAB (R120G)-related cardiomyopathy.

CryAB, known as Alpha-B Crystallin, has also been shown to bind and increase the unfolding force of the filamentous protein. Mutation of CryAB at R120G causes serious cardiomyopathy but lacks ideal therapeutic drugs. In the present study, we constructed CryAB (R120G)-transgenic (TG) mice.

Tunable Detection of MicroRNA-21 in Cancer Cells Using a 3D DNA Walker Mediated by Remote Toehold Strand Displacement Reaction.

Finely tunable detection of microRNAs (miRNAs) is crucial for personalized medicine and cancer diagnosis, and it is a challenge to construct a tunable sensor for miRNA detection due to the diversity and complexity of cancer patient samples. This study introduces a three-dimensional (3D) DNA walker mediated by a remote toehold strand displacement reaction, which addresses the challenge of achieving tunable detection limits and sensitivities for microRNA-21 (miR-21) at a kinetic level, enabling specific recognition of cancer cells. By engineering a spacer domain within the DNA walker, the kinetics of the strand displacement reaction can be precisely modulated, thereby controlling the walking efficiency and achieving tunable detection with a detection limit range from 32 aM to 290 pM and a broad dynamic range from ∼1100-fold to ∼283,000-fold.

Stratification and deposition pathways of microplastics in the abyssal sediments of the Philippine Sea.

Microplastics (MPs) have penetrated even the most remote deep-sea sediments, yet their vertical distribution and depositional pathways remain unclear. This study analyzed sediment cores (0-20 cm) from 11 Philippine Sea stations, finding MPs in all layers with an average abundance of 218.4 ± 303.

Occurrences, profiles, and mass inventories of sediment p-phenylenediamine and their quinones in the northern South China Sea.

p-Phenylenediamines (PPDs) and their quinones (PPD-Qs) have attracted increasing attention due to their widespread environmental occurrence, toxicity, and potential ecological risk. However, knowledge about their distribution in marine sediment is limited. In the present study, 47 sediment samples were collected from the northern South China Sea (NSCS) and Southwestern Sub-Basin (SWSB) of the SCS and analyzed for eight PPDs and eight PPD-Qs, from which a thorough analysis was conducted to address the above-mentioned issue.

[Identification of tissue distribution components and mechanism of antipyretic effect of famous classical formula Dayuanyin].

Based on the ultra performance liquid chromatography-quadrupole Exactive Orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS) technology, combined with related literature, databases, and reference material information, this study qualitatively analyzed the components of Dayuanyin in the tissue of rats after gavage and employed molecular docking technology to predict the rationality of the mechanism behind the antipyretic effect of the in vivo components in Dayuanyin. A total of 21, 26, 20, 21, 14, and 31 prototype components and 3, 16, 3, 7, 5, and 24 metabolites were identified from the heart, liver, spleen, lung, kidney, and hypothalamus of the rats, respectively, and the binding ability of key components and targets was further verified by molecular docking. The results showed that all components had good binding ability with targets.

Epidemiological intricacies of respiratory pathogens: a single-center study on infection dynamics in Beijing, 2023-2024.

Background: Following China's post-COVID-19 reopening strategy, intermittent small-scale outbreaks of respiratory pathogen infections have been observed in the Beijing region. This single-center study aims to characterize the epidemiological features and co-detection patterns of respiratory pathogens in Beijing, providing a scientific basis for the prevention and control of respiratory infectious diseases.

Methods: We performed a retrospective analysis of 19,535 patients undergoing SARS-CoV-2 testing and 12,372 patients screened for six respiratory pathogens, including (MP), respiratory syncytial virus (RSV), human rhinovirus (HRV), influenza A virus (IAV), adenovirus (ADV), and influenza B virus (IBV) at Peking University People's Hospital from January 2023 to December 2024.

Genome-Wide CRISPR-Cas9 Knockout Screening Identifies Genes Modulating Cisplatin-Induced Cytotoxicity in Renal Proximal Tubule Epithelial Cells.

Cisplatin is widely used as a first-line chemotherapy drug for various cancers. However, cisplatin-induced nephrotoxicity (CIN) greatly restricts its application. Renal proximal tubular epithelial cells (RPTECs) can be extensively damaged during CIN.

Antimitochondrial antibodies in systemic sclerosis target enteric neurons and are associated with GI dysmotility.

Objectives: Most patients with systemic sclerosis (SSc) experience gastrointestinal (GI) dysmotility. The enteric nervous system (ENS) regulates GI motility, and its dysfunction causes dysmotility. A subset of SSc patients harbour antimitochondrial M2 autoantibodies (AMA).

An accurate and sensitive method for neurotoxicity assessment of emerging contaminants in zebrafish.

The rapid increase in emerging contaminants driven by industrialization poses significant threats to both ecosystems and neurodevelopmental health, underscoring the urgent need for sensitive and accurate screening platforms for developmental neurotoxic compounds. Here, we developed a zebrafish-based multi-indicator assessment system to overcome the limitations of conventional behavioral assays. The morphologies of the head, actions of microglial cells, numbers of motor neurons, changes in neuronal activity and behavioral mobility of zebrafish larvae are examined and set as assessment criteria.

Effects of Oxygenation Resuspension on DOM Composition and Its Role in Reducing Dissolved Manganese in Drinking Water Reservoirs.

Anaerobic conditions in source water sediments are a key driver of manganese (Mn) release in drinking water systems. Enhancing sediment oxidation can inhibit Mn release, but the mechanisms of Mn speciation under varying oxidative conditions remain unclear. This study examined sediment exposure to oxygenated water layers at controlled dissolved oxygen levels (0, 2, 5, 7 mg L) through laboratory simulations.

Heart ultrasound and biomechanical evaluation of radiation-induced heart toxicity using transthoracic echocardiogram (TTE) and dynamic mechanical analysis (DMA).

Radiation-induced heart disease (RIHD) is a serious complication but difficult to assess in patients undergoing thoracic radiotherapy (RT). We aim to analyze RIHD using heart ultrasound and elastic modulus, exploring relationships between functional, anatomical or biomechanical changes of the heart and radiation dose. Twenty BALB/c mice were divided into four groups (control, 10 Gy, 20 Gy and 25 Gy) with a single fraction of image-guided volumetric modulated arc radiotherapy (VMAT) to murine heart on a linear accelerator.

Correlation of glycosylated apolipoprotein A1 and glycosylated low-density lipoprotein cholesterol levels with glucose homeostasis and the risk of developing type 2 diabetes mellitus.

Introduction: This study explored the relationship between Glycosylated apolipoprotein A-1 (G-apoA1) and glycosylated low-density lipoprotein cholesterol (G-LDL-C) levels and the risk of developing type 2 diabetes mellitus (T2DM).

Methods: This study included 3,098 patients with prediabetes and T2DM from two centers. Over a 3-year follow-up period, the study analyzed and assessed the risk of developing T2DM based on G-apoA1 and G-LDL-C levels.

A New Cryptic Species of Calotes (Reptilia: Squamata: Agamidae) from Southeastern Xizang Autonomous Region, China.

Based on both morphological and genetic data, we describe a new cryptic species of Calotes from southeastern Xizang Autonomous Region, China. The new species was confused with C. paulus, and it is morphologically most similar to members of the C.

The Benefits and Safety of Monoclonal Antibodies: Implications for Cancer Immunotherapy.

Monoclonal antibodies (mAbs) have transformed cancer treatment by providing highly targeted and effective therapies that specifically attack cancer cells, thus reducing the likelihood of adverse events (AEs) in patients. mAbs exert their action through various mechanisms, such as receptor blockade, antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and inhibition of immune checkpoints (eg, PD-1, PD-L1, and CTLA-4). These therapies have led to significant improvements in the treatment of several cancers, including HER2-positive breast cancer, non-small cell lung cancer (NSCLC), and melanoma.

Cross-sectional study of the association between triglyceride glucose-body mass index and obstructive sleep apnea risk.

Background: The triglyceride glucose-body mass index (TyG-BMI) is a novel indicator of insulin resistance (IR). Obstructive sleep apnea (OSA) is a prevalent disorder characterized by recurrent complete or partial collapse of the pharyngeal airway during sleep; however, the relationship between these two conditions remains unexplored. We hypothesized that a higher TyG-BMI is associated with the occurrence of OSA.

Myocardial intramural course may reduce left ventricular ejection fraction of patients suffering from coronary heart disease.

Background: Myocardial intramural course (MIC), a benign anatomical lesion, is an abnormal anatomical structure formed due to abnormal blood vessel routing. An increasing number of studies indicate that MIC is associated with coronary heart disease (CHD). However, it remains unclear whether MIC contributes to cardiac function impairment in patients with CHD.

The correlation between proprotein convertase subtilisin/kexin type 9 and adiponectin in the progression from prediabetes to type 2 diabetes mellitus.

Data on the involvement of proprotein convertase subtilisin/kexin type 9 (PCSK9) and adiponectin in prediabetes progression to type 2 diabetes mellitus (T2DM) remains inconclusive. Therefore, this study investigated the roles of PCSK9 and adiponectin in this process. This study included 1,528 participants with prediabetes and T2DM and conducted correlation analyses to investigate the relationship between PCSK9 and adiponectin levels, pancreatic beta-cell function, and insulin levels.

Green light suppresses cell growth but enhances photosynthetic rate and MIB biosynthesis in PE-containing Pseudanabaena.

2-Methylisoborneol (MIB) is a notorious musty odorant in drinking water systems, produced by cyanobacteria during the biosynthesis of photosynthetic pigments. This study investigated the physiological adaptation of Pseudanabaena cinerea, a phycoerythrin (PE)-containing and MIB-producing cyanobacterium, by inducing chromatic acclimation under different light color. Our findings revealed that red light enhanced growth rates by stimulating the tricarboxylic acid (TCA) cycle and associated metabolic processes, while green light significantly increased photosynthetic pigment content and electron transport efficiency.

[Pharmacokinetics study of Dayuanyin in normal and febrile rats].

Based on the pharmacokinetics theory, this study investigated the pharmacokinetic characteristics of albiflorin, paeoniflorin, wogonoside, and wogonin in normal and febrile rats and summarized absorption and elimination rules of Dayuanyin in them to provide reference for further development and clinical application of Dayuanyin. Blood samples were taken from the fundus venous plexus of normal and model rats after intragastric administration of Dayuanyin at different time points. The concentration of each substance in blood was determined by ultra performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-MS/MS) technique at different time points.

Single-Cell Array Enhanced Cell Damage Recognition Using Artificial Intelligence for Anticancer Drug Discovery.

This work developed a cell damage recognition method based on single-cell arrays using an artificial intelligence tool. The method uses micropatterns (single-cell micropatches and microwells) to isolate each cell in an ordered array to minimize cell overlapping and to maintain cell contours. After exposure to a therapeutic drug (e.

Jatrorrhizine Exhibits an Antitumor Effect Against Gefitinib-resistant Non-small Cell Lung Cancer via Inhibiting PI3K/mTOR Phosphorylation.

Epidermal growth factor receptor (EGFR) L858R/T790 M mutation-mediated gefitinib resistance (GR) is a frequent dilemma in the treatment of non-small cell lung cancer (NSCLC). This study aimed to explore the effect of jatrorrhizine on treating GR NSCLC and its possible mechanism of action. Cell viability, migration, invasion, and apoptosis detection were used to study the effect of jatrorrhizine on suppressing H1975 cells.

A Database on Antibiotics and Antibiotic Resistance in Wastewater and Solid Waste from Pharmaceutical Industry Based on a Systematic Review.

Residual antimicrobial agents in wastewater and solid waste from antimicrobial manufacturing facilities can potentially contaminate environments. The World Health Organization has established technical guidelines for managing antimicrobial resistance (AMR) in pharmaceutical wastewater and solid waste. However, the scarcity of publicly available data on antimicrobial manufacturing processes impedes the development of effective mitigation strategies.

Design of a Co doped carbon Backbone with self-grown Au nanoparticles via a 'Triple Advantage' Strategy for sensitive dopamine detection.

In this study, a Co doped polyhedral carbon skeleton (Co CN) was prepared by nitrogen carbonization using ZIF-67 as a precursor. The Co CN features a rough surface with excellent electrical conductivity, and the Co atoms exhibit unique catalytic properties. Based on these characteristics, we used Co CN as a carrier to load Au nanoparticles (NPs) onto its surface through the linkage and reduction effects of polyoxometalates (POMs).