Clinical effectiveness of ethinylestradiol-cyproterone acetate and raloxifene combination therapy in polycystic ovary syndrome-associated infertility.

Objective: To evaluate the therapeutic outcomes of combining ethinylestradiol-cyproterone acetate (EE-CPA) with raloxifene in the management of polycystic ovary syndrome (PCOS)-associated infertility.

Methods: The study included 115 women diagnosed with PCOS-associated infertility, admitted between January 2021 and January 2025. The participants were divided into two groups: 55 women receiving raloxifene monotherapy (control group) and 60 treated with EE-CPA plus raloxifene (observation group).

Factors associated with mpox awareness among men who have sex with men recruited through the internet: a cross-sectional survey in China.

Objective: This study aimed to provide a scientific basis for formulating mpox prevention and control strategies for men who have sex with men (MSM) by analyzing the associated factors.

Methods: We conducted online surveys with individuals recruited through the Internet in May 2024. Univariate and multivariate logistic regression analyses were used to identify factors influencing knowledge about mpox.

Natural-Language-Interfaced Robotic Synthesis for AI-Copilot-Assisted Exploration of Inorganic Materials.

The automation of chemical synthesis presents opportunities to enhance experimental reproducibility and accelerate discovery. Traditional closed-loop approaches, while effective in specific domains, are often constrained by rigid workflows and the requirement for specialized expertise. Here, we introduce a chemical robotic explorer integrated with an artificial intelligence (AI) copilot to enable a more flexible and adaptive synthesis, simplifying the process from inspiration to experimentation.

Bioinspired Camouflage Fibers with Computer Vision-Guided Chromatic Adaptation.

The development of intelligent camouflage systems demands advanced materials and control strategies for dynamic environmental adaptation. Here, we demonstrate a bioinspired camouflage system using hydroxypropyl cellulose (HPC), a cellulose derivative that forms cholesteric liquid crystals with mechanically tunable structural colors. By integrating HPC fibers with computer vision-assisted control, we achieve autonomous color matching with the environment through precise mechanical manipulation.

Optimising colorectal cancer screening strategies and target populations in budget-constrained regions through cost-effectiveness analysis: a case from eastern China.

Objectives: The primary aim of this study was to optimise colorectal cancer (CRC) screening strategies and target populations in resource-limited areas through cost-effectiveness analysis, evaluating the best screening methods and appropriate screening ages.

Design: A prospective microsimulation model was used for cost-effectiveness analysis, calibrated with real-world data.

Setting: The study was conducted in Huzhou City, Zhejiang Province, China, focusing on primary and secondary healthcare levels.

Constructing machine learning-based risk prediction model for osteoarthritis in population aged 45 and above: NHANES 2011-2018.

Osteoarthritis is a widespread chronic joint disease, becoming increasingly prevalent, particularly among individuals over the age of 45. This condition causes joint pain and dysfunction, significantly disrupting daily life. The objective of this study is to develop an optimal machine learning model for predicting the risk of osteoarthritis in individuals aged 45 and older.

The complete mitochondrial genome of the grasshopper (Zub.) (Orthoptera: Acrididae: Gomphocerinae): detailed characterization and phylogenetic position.

(Zub.) is one of the dominant grasshopper species. The limited data on (Zub.

Engineered bioorthogonal cell delivery system for in situ antimicrobial peptide recruitment during systemic bacterial infection.

Allogeneic cells represent promising intelligent delivery platforms owing to their intrinsic target homing ability, ready-to-use, scalability and broad applicability. However, implanted allogeneic cells are susceptible to rapid clearance by mononuclear phagocytic system (MPS), resulting in short half-life and compromised therapeutic efficacy. To overcome this limitation, we constructed genetically engineered allogeneic cells with surface-expressed CD24, a "don't eat me" signal protein, to evade phagocytosis by macrophages.

Palladium-Catalyzed Domino Heck/Decarboxylative Cyanomethylation of Indoles and Alkenes with Cyanoacetate Salts.

A palladium-catalyzed reaction of indoles with cyanoacetate salts enables the synthesis of 2,6-disubstituted indolines via tandem dearomatization/decarboxylative cyanomethylation. Remarkably, this is the first example of indole difunctionalization at the C2 and C6 positions. Moreover, this methodology extends to the palladium-catalyzed cyclization/decarboxylative cyanomethylation of aryl halide-tethered alkenes.

Description and Phylogenetic Studies on the Complete Mitochondrial Genome of Kingdonella qinghaiensis Zheng (Insecta: Orthoptera: Acrididae).

Kingdonella qinghaiensis Zheng, 1990 a species belonging to the genus Kingdonella Uvarov, 1933, within the family Acrididae in the order Orthoptera, is mainly distributed in Qinghai province, China. In this study, we determined, assembled and annotated the mitochondrial genome of Kingdonella qinghaiensis. The mitogenome is 15,597 bp in length and contains 13 protein-coding genes (PCGs), 22 tRNA genes, 2 rRNA genes and a control region (D-loop).

Metformin sensitizes triple-negative breast cancer to histone deacetylase inhibitors by targeting FGFR4.

Background: Triple-negative breast cancer (TNBC) is characterized by high malignancy, strong invasiveness, and a propensity for distant metastasis, leading to poor prognosis and relatively limited treatment options. Metformin, as a first-line oral hypoglycemic agent, has garnered widespread research interest in recent years due to its potential in cancer prevention and treatment. However, its efficacy varies significantly across different tumor types.

Cost-effectiveness analysis of sequential two-step screening versus direct colonoscopy screening for colorectal cancer: a large-scale survey in Eastern China.

Objectives: Despite the implementation of colorectal cancer (CRC) screening programs in many regions worldwide over the past few decades, the cost-effectiveness of these programs has been questioned owing to their acceptance rates. In this study, we evaluated the cost-effectiveness of screening strategies, quantified the impact of colonoscopy acceptance rates, and analyzed the underlying factors driving individual preferences.

Methods: The cost-effectiveness of three strategies-no screening, sequential two-step screening (fecal immunochemical test and risk assessment, followed by colonoscopy), and colonoscopy screening-was evaluated from a societal perspective.

Single-Cell Array Enhanced Cell Damage Recognition Using Artificial Intelligence for Anticancer Drug Discovery.

This work developed a cell damage recognition method based on single-cell arrays using an artificial intelligence tool. The method uses micropatterns (single-cell micropatches and microwells) to isolate each cell in an ordered array to minimize cell overlapping and to maintain cell contours. After exposure to a therapeutic drug (e.

Factors associated with having a steady homosexual partner among men who have sex with men, based on internet recruitment.

Objective: This study analyzed the status and factors associated with having a steady homosexual partner (HP) among men who have sex with men (MSM) to provide a scientific basis for developing prevention and control strategies for acquired immunodeficiency syndrome in this population.

Methods: A questionnaire survey was conducted among study participants who were MSM recruited online by a non-governmental organization in May 2024. The participants were divided into two groups based on whether they had a steady HP.

Fast food and anxiety symptoms of Chinese multi-ethnic adolescents: The moderating role of regulatory emotional self-efficacy.

Background: Although previous studies demonstrated an association between fast food intake and anxiety symptoms in adolescents, this relationship has not been studied in Chinese multi-ethnic adolescents. The purpose of this study is to investigate the aforementioned association and the potential moderating effect of regulatory emotional self-efficacy (RESE) among Chinese multi-ethnic adolescents.

Methods: A cluster random sampling of 8500 middle school students from 11 counties in Yunnan province was conducted.

Performance of a Full-Coverage Cervical Cancer Screening Program Using on an Artificial Intelligence- and Cloud-Based Diagnostic System: Observational Study of an Ultralarge Population.

Background: The World Health Organization has set a global strategy to eliminate cervical cancer, emphasizing the need for cervical cancer screening coverage to reach 70%. In response, China has developed an action plan to accelerate the elimination of cervical cancer, with Hubei province implementing China's first provincial full-coverage screening program using an artificial intelligence (AI) and cloud-based diagnostic system.

Objective: This study aimed to evaluate the performance of AI technology in this full-coverage screening program.

Connexin 43 controls metastatic behavior in triple negative breast cancer through TGFβ1-Smad3-intergin αV signaling axis Based on optical image diagnosis.

Abnormal expression of connexin 43 (Cx43) contributes to the development and progression of cancer. However, its regulation is complex and dependent on the environment. The expression of Cx43 in triple-negative cancer lesions was analyzed by immunohistochemistry and optical coherence tomography using experimental models and clinical samples.

Olanzapine Induces Adipogenesis and Glucose Uptake by Activating Glycolysis and Synergizing with the PI3K-AKT Pathway.

Background: Administration of olanzapine (OLA) is closely associated with obesity and glycolipid abnormalities in patients with schizophrenia (SCZ), although the exact molecular mechanisms remain elusive.

Objective: We conducted comprehensive animal and molecular experiments to elucidate the mechanisms underlying OLA-induced weight gain.

Methods: We investigated the mechanisms of OLA-induced adipogenesis and lipid storage by employing a real-time ATP production rate assay, glucose uptake test, and reactive oxygen species (ROS) detection in 3T3-L1 cells and AMSCs.

Advances in Developing Small Molecule Drugs for Alzheimer's Disease.

Alzheimer's disease (AD) is the most common type of dementia among middle-aged and elderly individuals. Accelerating the prevention and treatment of AD has become an urgent problem. New technology including Computer-aided drug design (CADD) can effectively reduce the medication cost for patients with AD, reduce the cost of living, and improve the quality of life of patients, providing new ideas for treating AD.

Factors associated with casual sexual behavior among college students in Zhejiang Province, China: A cross-sectional survey.

This study aimed to analyze the characteristics and risk factors associated with casual sexual behavior among sexually active college students and to contribute to AIDS prevention and control efforts among this demographic. A cross-sectional survey was conducted using a stratified cluster sampling technique. Self-reported sexually active college students were selected as respondents from 11 cities in Zhejiang Province from October 8 to November 30, 2018.

A proximity labeling-based orthogonal trap strategy identifies HDAC8 promotes cell motility by modulating cortactin acetylation.

It is a challenge for the traditional affinity methods to capture transient interactions of enzyme-post-translational modification (PTM) substrates in vivo. Herein we presented a strategy termed proximity labeling-based orthogonal trap approach (ProLORT), relying upon APEX2-catalysed proximity labeling and an orthogonal trap pipeline as well as quantitative proteomics to directly investigate the transient interactome of enzyme-PTM substrates in living cells. As a proof of concept, ProLORT allows for robust evaluation of a known HDAC8 substrate, histone H3K9ac.

Histone deacetylase inhibitors VPA and WT161 ameliorate the pathological features and cognitive impairments of the APP/PS1 Alzheimer's disease mouse model by regulating the expression of APP secretases.

Background: Alzheimer's disease (AD) is a degenerative neurological disorder. Recent studies have indicated that histone deacetylases (HDACs) are among the most prominent epigenetic therapy targets and that HDAC inhibitors have therapeutic effects on AD. Here, we identified sodium valproate (VPA), a pan-HDAC inhibitor, and WT161, a novel HDAC6 selective inhibitor, as potential therapeutic agents for AD.

Temporal and spatial variations of disinfection by-products in South Taihu's drinking water, Zhejiang Province, China.

Some disinfection by-products (DBPs) in drinking water present a potential safety concern. This study focuses on the elements influencing DBPs formation. A total of 120 water samples were collected from 10 different drinking water facilities spanning 5 counties within Huzhou, Zhejiang Province, China.

Ferritinophagy-mediated apoptosis and paraptosis induction involved MAPK and PI3K/AKT pathway in mechanism of an iron chelator.

Melanoma cells were more resistant to ferroptosis with still poor therapy outcomes. Sensitizing melanoma cell to the ferroptosis inducer was a crucial strategy for treatment of melanoma. In the present study, 2,2'-di-pyridylketone hydrazone dithiocarbamate s-butyric acid (DpdtbA) displayed superior inhibitory activity than ferroptosis inducer Erastin in melanoma cells, which prompt us to explore the underlying mechanism.