Comprehensive bioinformatic analysis identifies potential therapeutic drugs for CryAB (R120G)-related cardiomyopathy.

CryAB, known as Alpha-B Crystallin, has also been shown to bind and increase the unfolding force of the filamentous protein. Mutation of CryAB at R120G causes serious cardiomyopathy but lacks ideal therapeutic drugs. In the present study, we constructed CryAB (R120G)-transgenic (TG) mice.

Phosphate ion functionalized Ni(OH)/Ni/MoO composite for enhanced alkaline hydrogen evolution.

The development of efficient electrocatalysts for hydrogen evolution reaction (HER) is important in advancing sustainable energy technologies. This work introduces a phosphate ion modified Ni(OH)/Ni/MoO (PNNM) composite, elaborately constructed by a one-pot electrodeposition method. The integration of heterostructure engineering and ion modification strategies significantly endows the composite with remarkable electrocatalytic performance.

Neutrophil-to-Albumin Ratio Predicts Stroke-Associated Pneumonia in Patients with Intracerebral Hemorrhage.

Background And Aim: Pneumonia is a significant complication that negatively impacts outcomes in patients with intracerebral hemorrhage (ICH). Identifying reliable biomarkers for predicting such infections is crucial for timely intervention and improving patient management. This study aims to evaluate the predictive value of neutrophil-to-albumin Ratio (NAR)for pneumonia in patients undergoing surgical intervention for ICH.

Bacterial K-strategists playing pivotal roles in degrading alkanes, upholding system stability and regulating microbial diversity in natural attenuation of petroleum hydrocarbon polluted sediment of Laizhou Bay.

Natural attenuation represents a pivotal process in the management of oil spills within marine ecosystems, one of which is to monitor bioprocess that is inseparable from microbial action. However, the behaviors of microorganism with different survival strategies under petroleum contamination remain largely unexplored in gulf environments. Herein, ecological and evolutionary characteristics of K- and r-strategist were comprehensively investigated in diesel-polluted sediment from Laizhou Bay via molecular sequencing and physicochemical experiments.

Adenosine triphosphate-triggered aggregable DNA micelles for triple enhanced photothermal therapy in the second near-infrared window.

Despite significant advancements in photothermal therapy (PTT), its clinical treatment efficacy for cancer remains constrained because of the limited tissue penetration of light as well as the thermal resistance of tumor cells. To achieve efficient PTT activated under near-infrared II (NIR-II) light irradiation, here we developed a triple-enhanced NIR-II PTT system through the design of ATP-triggered aggregable DNA micelle functionalized with mitochondrial-targeting and protein expression inhibition. The first PTT enhancement was achieved through adenosine triphosphate (ATP) induced hybridization chain reaction between micelles, which lead to the micelle aggregation and improve the local heating effect.

Synthetic holographic mask design for computational proximity lithography using the lithography-guided OMRAF method.

Mask aligner lithography does not rely on complex projection lens systems, offering a cost-effective and high-yield approach for chip manufacturing. However, its limited resolution restricts application in advanced chip manufacturing. Holographic lithography enhances lithographic resolution by introducing phase information onto the mask.

Case Report: Allogeneic adipose-derived mesenchymal stem cells for severe feline chronic kidney disease.

A 10-year-old neutered male Ragdoll cat presented with symptoms of anorexia, nausea, vomiting, lethargy, and progressive weight loss. Laboratory parameters and clinical signs led to a diagnosis of International Renal Interest Society (IRIS) Chronic Kidney Disease (CKD) Stage III. Following basic supportive therapy, clinical symptoms subsided, and the patient was discharged for home management.

Discovery of Diverse Seven-Membered Ring Vibsane Diterpenoids from Utilizing a MassQL-Integrated Strategy and Their Antihepatoma Effect.

Through a MassQL-integrated strategy, 26 vibsane diterpenoids (VibDs) were identified from , including 15 new compounds () and 11 known analogues (). Compound possessed an unprecedented seven-membered ring scaffold, representing a new type of VibD. The hypothetical biosynthetic pathway of was proposed.

Catalytic synthesis of chiral sulfinimidate esters via oxidative esterification of sulfenamides.

Aza-sulfur compounds, such as sulfilimines, sulfoximines, etc, are increasingly recognized as essential contributors to advancements in drug development and asymmetric synthesis. Among them, the catalytic enantioselective synthesis of sulfinimidate esters remains an uncharted and formidable challenge. Herein, we unveil an efficient enantioselective oxidative esterification of sulfenamides via chiral sulfinimidoyl iodide intermediates.

Exogenous Application of the Functional Metabolite Rosmarinic Acid Can Simulate the Effects of AMF Inoculation on Plant Growth Promotion and Cd Passivation.

Previous studies revealed that inoculation with Glomus mosseae (AMF) significantly increased biomass and reduced cadmium (Cd) accumulation in Cd-sensitive Medicago sativa, which was linked to AMF-induced root exudates recruiting a functional microbiome enriched with plant growth-promoting and Cd-passivating bacteria. Here, we employed microcosm and greenhouse pot experiments to identify key metabolites that enhance the α diversity and functional potential of this microbiome and evaluated their exogenous application effects. Microcosm screening, based on α diversity and functional profiling of growth-promoting and Cd-passivating bacterial communities, identified rosmarinic acid, myristic acid, and indole-3-acetic acid (IAA) as critical functional metabolites.

B cells drive CCR5CD4 tissue-resident memory T cell cytotoxicity via IL-15Rα-IL-15 signaling in primary biliary cholangitis.

Background & Aims: IL-15Rα mediates immune responses by binding and trans-presenting IL-15 to tissue-resident memory T (T) cells, enabling sustained activation. Given the pivotal role of IL-15Rα/IL-15 signaling in autoimmune diseases, elucidating its organ-specific expression and pathogenic functions in primary biliary cholangitis (PBC) remains crucial.

Methods: Immunohistochemical staining was performed on liver tissues from patients with PBC (n=94), AIH (n=32), CHB (n=10), NASH (n=7) and HCs (n=10) to assess IL-15Rα expression.

Bioactive sesquiterpene lactones from Elephantopus scaber: semi-synthesis, target fishing, anti-malignant glioma efficacy in vitro and in vivo.

Traditional Chinese Medicine (TCM) offers distinct advantages in the treatment of tumors, since it serves dually as both a medicinal treatment and a dietary therapy. Elephantopus scaber (E. scaber), with a plethora of folk medicinal usage for treating pneumonia and hepatitis, contains sesquiterpene lactone (SL) as the primary component for therapeutic efficacy.

Prehospital blood pressure lowering in patients with ultra-acute presumed stroke: A systematic review and meta-analysis.

Objective: High blood pressure frequently occurs in the setting of acute stroke and is associated with worse prognoses. However, it is still uncertain whether initiating blood pressure-lowering therapy in the prehospital phase after stroke onset can enhance outcomes for patients with undifferentiated acute stroke.

Methods: We conducted a search of the PubMed, Embase, and Cochrane databases to identify randomized controlled trials investigating prehospital blood pressure lowering interventions for presumed ultra-acute stroke (within <6 hours).

Neutrophil to high-density lipoprotein cholesterol ratio predicts deep venous thrombosis in spontaneous intracerebral hemorrhage.

Deep vein thrombosis (DVT) is a common and serious complication of spontaneous intracerebral hemorrhage (ICH). The timely recognition and treatment of DVT can significantly mitigate several negative consequences. To investigate the association between the neutrophil-to-high-density lipoprotein cholesterol ratio (NHR) and the occurrence of DVT in ICH patients.

Association Between Neutrophil Percentage-to-Albumin Ratio and Neutrophil-to-Albumin Ratio with Long-Term Mortality in Patients with Intracerebral Hemorrhage.

Background: The association between the neutrophil percentage-to-albumin ratio (NPAR) and neutrophil-to-albumin ratio (NAR) with long-term mortality in patients with intracerebral hemorrhage (ICH) remains underexplored.

Methods: We conducted a retrospective cohort study, including patients diagnosed with ICH from 2 tertiary care centers. NPAR and NAR levels were calculated from blood samples obtained within 24 hours of admission.

Virtual Hydrolysis-Based Screening of Wheat-Derived DPP-IV Inhibitory Peptides: A Mechanistic Analysis Integrating Cell Experiments and Molecular Dynamics Simulations.

Dipeptidyl peptidase-IV (DPP-IV) inhibitors play a critical role in the treatment of diabetes and metabolic diseases. This study combines computational simulations with experimental validation to identify peptides with potential DPP-IV inhibitory activity from wheat proteins. A peptide database was constructed through trypsin digestion simulation, and screening was performed using the ConPLex deep learning algorithm, leading to the identification of four promising peptides: TENEWK (Thr-Glu-Asn-Glu-Trp-Lys), NFVSER (Asn-Phe-Val-Ser-Glu-Arg), LDLPSK (Leu-Asp-Leu-Pro-Ser-Lys) and QHEQR (Gln-His-Glu-Gln-Arg).

Experimental study of the effect of natural fracture curvature on hydraulic fracture propagation behavior.

The interaction mechanism between hydraulic fractures (HF) and natural fractures (NF) constitutes a critical research focus in hydraulic fracturing optimization. This study systematically investigates the influence of NF curvature on HF propagation behavior through large-scale true triaxial hydraulic fracturing physical simulations. The experiments were conducted on artificial rock specimens containing prefabricated fractures with varying curvature parameters.

Novel insights into quorum-sensing microbe combined with dimethylsulfoniopropionate enhancing bioremediation of oil-contaminated sediment under typical deep-sea conditions: The performances and molecular mechanisms.

The deep-sea environment poses significant metabolic challenges to microorganisms, rendering the bioremediation of oil pollution even more formidable. Quorum sensing (QS) signals are known to assist microbiota in maintaining their function and stability under toxic contaminants. Dimethylsulfoniopropionate (DMSP) is an ideal cryoprotectant and stress protectant, enhancing microbial resilience in extreme environments.

Enhancing Hydrogen Generation by Anodic Oxidation of Urea over Superb Hydrophilic Hierarchical Ni, S co-Doped Co(PO) Nanosheets.

Surface wettability are of great significance to electrocatalysts for various electrochemical reaction. In this work, Ni, S co-doped Co(PO) nanosheets on Ni foam (Ni-S-Co(PO) NSs) with high hydrophilicity and hierarchical structure are synthesized. The doping of S is the key factor to achieve an ultrahigh hydrophilic surface, identified by the contact angle measurement.

Methyl-functionalized anionic MOFs for charge- and shape-selective adsorption of dyes in water remediation.

A methyl-functionalized MOF exhibits dual-selective adsorption of linear cationic dye molecules electrostatic attraction and steric discrimination, which are based on the anionic framework and methyl-induced pore confinement of the MOF. Its capacity to adsorb methylene blue is as high as 232.98 mg g, surpassing that of the parent MOF.

Clinical characteristics and antibiotic sensitivity & resistance analysis of cases of orthopedic infections caused by Aeromonas hydrophila.

Objective: This study was to elucidate the clinical characteristics and antibiotic susceptibility of orthopedic infections caused by Aeromonas hydrophila, thereby providing a suggestion for early clinical recognition and optimized managementof such infections in orthopedic clinical practice.

Methods: A retrospective investigation was conducted by collecting general patient information, specimen sources, culture results, and antibiotic susceptibility data from 58 cases of Aeromonas hydrophila infections treated at our hospital between November 2020 and April 2024.

Results: The 58 patients with Aeromonas hydrophila infections had a mean age of (50.

The phenotypic and functional characteristics of intrahepatic CD69CD103 tissue-resident MAIT cells in primary biliary cholangitis.

Background & Aims: Mucosal-associated invariant T (MAIT) cells are an innate-like T cell subset that plays a significant role in the immunopathology of primary biliary cholangitis (PBC). However, our understanding of the subpopulations involved in hepatic residency have not been elucidated. Herein, our goal was to delineate the phenotypic and functional properties of intrahepatic tissue-resident MAIT cells in PBC.

Study on the mechanism of acteoside in treating purinomycin aminonucleoside-induced chronic glomerulonephritis in childhood rats based on Cxcr4-PI3K-Akt-eNOS axis.

The diagnosis and treatment of chronic glomerulonephritis (CGN) during childhood pose distinct challenges. Acteoside (ACT) is the primary active ingredient extracted from the leaves of Rehmannia glutinosa by our research group, accounting for 2.15 %, which possess multiple biological activities, especially for nephropathy treatment.

Neutrophil-to-albumin ratio as a predictor of mortality in patients with intracerebral hemorrhage: a multicenter retrospective cohort study.

Background: Intracerebral hemorrhage (ICH) is associated with a poor prognosis. The association between the neutrophil-to-albumin ratio (NAR) with mortality in patients with ICH remains underexplored. This study investigated the relationship between the NAR and mortality in patients with ICH.