Tunable Detection of MicroRNA-21 in Cancer Cells Using a 3D DNA Walker Mediated by Remote Toehold Strand Displacement Reaction.

Finely tunable detection of microRNAs (miRNAs) is crucial for personalized medicine and cancer diagnosis, and it is a challenge to construct a tunable sensor for miRNA detection due to the diversity and complexity of cancer patient samples. This study introduces a three-dimensional (3D) DNA walker mediated by a remote toehold strand displacement reaction, which addresses the challenge of achieving tunable detection limits and sensitivities for microRNA-21 (miR-21) at a kinetic level, enabling specific recognition of cancer cells. By engineering a spacer domain within the DNA walker, the kinetics of the strand displacement reaction can be precisely modulated, thereby controlling the walking efficiency and achieving tunable detection with a detection limit range from 32 aM to 290 pM and a broad dynamic range from ∼1100-fold to ∼283,000-fold.

Corrosion characteristics of low-carbon cast steel ZG230-450 in chloride-sulfate atmospheric environment.

In the present paper, the corrosion behavior of ZG230-450 in chloride-sulfate atmospheric environment was studied, comparing with ordinary low-carbon steel Q235. Long-term and short-term corrosion tests were conducted, the corrosion products of the materials were observed and tested by scanning electron microscope and X-ray diffraction, and the corrosion rate and pitting corrosion behavior of the materials were studied by electrochemical test. The test results show that (1) the corrosion products of Q235 are mainly composed of γ-FeO, γ-FeOOH, and α-FeOOH, while corrosion products of ZG230-450 are mainly composed of FeO, γ-FeOOH, and α-FeOOH; (2) in the early stage of corrosion, the corrosion rate of ZG230-450 is higher than that of Q235.

Knockdown of annexin A2 enhances the host cell apoptosis induced by .

Annexin A2 (ANXA2) is a multifunctional protein involved in host-pathogen interactions during viral and parasitic infections. To investigate the role of ANXA2 in host cell apoptosis induced by , RNA interference (RNAi) was employed to specifically downregulate ANXA2 expression. Primary cultures of chicken embryonic cecal epithelial cells were established and subjected to sporozoite infection.

Alkaline Earth Metal-Based Hybrid Organic-Inorganic Perovskite-Like Ferroelectrics.

Hybrid organic-inorganic perovskites (HOIPs) with the ABX formula have garnered significant scientific interest owing to their substantial technological promise in photovoltaics, optoelectronics and ferroelectrics. Their structural diversity and molecular-scale chemical programmability provide an exceptional platform for engineering diverse functional HOIP architectures through targeted compositional design. However, the strategic potential of alkaline earth metals (e.

Echinococcosis on the Tibetan Plateau, where to go?

The post-COVID-19 era has exacerbated challenges in controlling echinococcosis on the Tibetan Plateau, the epicentre of alveolar and cystic echinococcosis, where reduced funding for neglected tropical diseases (NTDs) coincides with growing tourism and trade. This convergence heightens transmission risk, and we provide a novel synthesis of context-specific, integrated control strategies.

From incidental to intentional: ultrasound imaging in detecting internal jugular vein duplication/fenestration from a case report.

Rationale: Few reports in the literature describe internal jugular vein fenestration or duplication, and these are typically incidental surgical findings, which include a few 3-dimensional radiological images of these anomalies obtained from preoperative imaging. The potential of ultrasound has been greatly underestimated because there is no previous literature on ultrasound-based diagnosis of this disease.

Patient Concerns: A 40-year-old woman presented with dizziness.

The value of nanocarbon contrast methylene blue based on dye-based tracer technology in sentinel lymph node biopsy for breast cancer: a systematic review and meta-analysis.

Objective: The purpose of this study was to compare the diagnostic value of nanocarbon suspensions and methylene blue injections in sentinel lymph node biopsies of patients with breast cancer based on the dye method.

Methods: A systematic search of the PubMed, Embase, Cochrane Library (Central) and Web of Science (SCI Expanded) databases was performed to determine the diagnostic value of carbon nanoparticles in Chinese databases (China National Knowledge Infrastructure (CNKI), VIP, Wanfang, Chinese Biomedical Literature Database (CBM)) for identifying methylene blue in sentinel lymph node biopsies of patients with breast cancer. The QUADAS2 quality evaluation tool of Review Manager 5.

Corrigendum: ADHD detection from EEG signals using GCN based on multi-domain features.

[This corrects the article DOI: 10.3389/fnins.2025.

[Retracted] Overexpression of microRNA‑155 alleviates palmitate‑induced vascular endothelial cell injury in human umbilical vein endothelial cells by negatively regulating the Wnt signaling pathway.

Following the publication of this paper, it was drawn to the Editors' attention by a concerned reader that the majority of the flow cytometric assay data shown in Fig. 4A on p. 3531 were strikingly similar to data appearing in different form in other articles written by different authors at different research institutes that had already been published elsewhere prior to the submission of this paper to (several of which have already been retracted).

Difficulty removing a totally implantable venous access port: A case report.

Background: This case report examines the challenges associated with removing a totally implantable venous access port (TIVAP) used for long-term chemotherapy in a patient with breast cancer. Prolonged use of TIVAPs can result in complications such as catheter kinking, thrombosis, and adhesions between the catheter and surrounding tissues, potentially complicating their removal.

Case Summary: A breast cancer patient with bone metastasis presented with difficulty aspirating blood from a TIVAP that had been placed in the right internal jugular vein for 3 years.

Enhanced extraction of microplastics from terrestrial animal intestinal tissues via optimized fenton oxidation.

Microplastics (MPs) are readily ingested by organisms and tend to accumulate in intestinal tissues, posing potential health risks. However, most existing MP extraction methods are designed for aquatic organisms and are unsuitable for terrestrial organisms with high lipid content. In this study, we developed an efficient Fenton oxidation-based method for extracting MPs from mouse intestinal tissues.

Effects of esketamine-sufentanil for patient-controlled intravenous analgesia in women following cesarean section: A randomized clinical trial.

Background: Postoperative pain following cesarean section can cause maternal anxiety, limited ambulation, and even postpartum depression. In this study, we aimed to investigate the effects of esketamine for postoperative patient-controlled intravenous analgesia in women following cesarean section.

Methods: One hundred women were randomly assigned to two groups.

ADHD detection from EEG signals using GCN based on multi-domain features.

Introduction: Attention deficit hyperactivity disorder (ADHD) is a common psychiatric disorder in children during their early school years. While many researchers have explored automated ADHD detection methods, developing accurate, rapid, and reliable approaches remains challenging.

Methods: This study proposes a graph convolutional neural network (GCN)-based ADHD detection framework utilizing multi-domain electroencephalogram (EEG) features.

Multi-objective decision-making considering future demands helps to delineate key areas for ecological restoration in Changsha-Zhuzhou-Xiangtan urban agglomeration, China.

ecological restoration is an effective method to address land degradation and ecosystem deterioration of urban agglomeration with rapid development. However, previous efforts neglect socio-economic goals for ecological restoration and the land use demand in the future, leading to a disconnect between ecological restoration and the support for social and economic development. Here we explore the identification of key areas for ecological restoration (KAsER) according to a multi-objective view of the urban agglomeration, as well as future demand.

SDF-1 alleviates osteoarthritis by resolving mitochondrial dysfunction through the activation of the Sirt3/PGC-1α signalling pathway.

Objective: Osteoarthritis (OA) is the most common form of joint disease. Currently, OA treatment is limited to controlling symptoms. Our previous study showed that stromal cell-derived factor 1 (SDF-1) delayed the progression of OA to a certain extent.

Development of Potent SHP2 Allosteric Inhibitors: Design, Synthesis, and Evaluation with Antitumor Effects.

Src homology-2-containing protein tyrosine phosphatase (PTP) 2 (SHP2) is a pivotal PTP that modulates key cellular processes including proliferation, differentiation, and migration. Its overexpression is implicated in the pathogenesis of various malignancies, highlighting the need for effective SHP2 inhibitors. Herein, we report the design and synthesis of a novel series of thiazolo[5,4-]pyridine and imidazo[1,2-]pyrimidine derivatives as SHP2 allosteric inhibitors identified through active fragment splicing.

Seasonal variations in groundwater chemistry and quality and associated health risks from domestic wells and crucial constraints in the Pearl River Delta.

Groundwater quality is strongly compromised by polluted surface water recharge in rapidly developing urban regions. However, gaps still remain in the understanding of the critical contaminants controlling water quality and the health risks associated with groundwater consumption, particularly considering seasonal and climate changes in rainfall. This work focused on changes in groundwater quality and critical contaminants in domestic wells in the fast-developing Pearl River Delta (PRD) from the wet season to the dry season.

Integrated computational analysis identifies therapeutic targets with dual action in cancer cells and T cells.

Many cancer drugs that target cancer cell pathways also impair the immune system. We developed a computational target discovery platform to enable examination of both cancer and immune cells so as to identify pathways that restrain tumor progression and potentiate anti-tumor immunity. Immune-related CRISPR screen analyzer of functional targets (ICRAFT) integrates immune-related CRISPR screen datasets, single-cell RNA sequencing (scRNA-seq) data, and pre-treatment RNA-seq data from clinical trials, enabling a systems-level approach to therapeutic target discovery.

A novel nomogram for predicting the risk of delayed onset lactogenesis II among women who delivered via cesarean section in China.

This study aimed to develop a prognostic nomogram to predict the risk of delayed onset lactogenesis II (DOL II) in Chinese women who delivered via cesarean section. A total of 143 women who delivered via cesarean section in our hospital between June 2021 and May 2022 were retrospectively reviewed. A nomogram was constructed using the independent predictors extracted from the logistic regression analysis.

Contribution of Active Surface of NiFe-Layered Double Hydroxide on the Removal of Methyl Orange.

Layered double hydroxides (LDHs) have potential applications for pollutant removal. Enhancing their pollutant removal ability by fully utilizing the synergistic effects of physical adsorption and chemical catalysis has received widespread attention. In this study, a high methyl orange (MO) removal capacity was achieved by utilizing the synergistic effects of physical adsorption and chemical catalysis of NiFe-LDH.

Spatiotemporal Transcriptomic Profiling Reveals the Dynamic Immunological Landscape of Alveolar Echinococcosis.

Alveolar echinococcosis (AE) is caused by the chronic infection of E. multilocularis, whose tumor-like growth can lead to high fatality if improperly treated. The early diagnosis of infection and the treatment of advanced AE remain challenging.

Optimal channel and feature selection for automatic prediction of functional brain age of preterm infant based on EEG.

Introduction: Approximately 15 million premature infants are born each year, many of whom face risks of neurological impairments. Accurate assessment of brain maturity is crucial for timely intervention and treatment planning. Electroencephalography (EEG) is a noninvasive method commonly used for this purpose.

NIRFluor: A Deep Learning Platform for Rapid Screening of Small Molecule Near-Infrared Fluorophores with Desired Optical Properties.

Small molecule near-infrared (NIR) fluorophores play a critical role in disease diagnosis and early detection of various markers in living organisms. To accelerate their development and design, a deep learning platform, NIRFluor, was established to rapidly screen small molecule NIR fluorophores with the desired optical properties. The core component of NIRFluor is a state-of-the-art deep learning model trained on 5179 experimental big data.

Endothelial FUNDC1 Deficiency Drives Pulmonary Hypertension.

Background: Pulmonary hypertension (PH) is associated with endothelial dysfunction. However, the cause of endothelial dysfunction and its impact on PH remain incompletely understood. We aimed to investigate whether the hypoxia-inducible FUNDC1 (FUN14 domain-containing 1)-dependent mitophagy pathway underlies PH pathogenesis and progression.