Pyrazinoquinazoline-Based Eu-MOF Ratiometric Fluorescence Sensing: Real-Time Monitoring of Meat Spoilage Enabled by Dynamic Regulation of LMCT/LLCT Triggered by Biogenic Amines.

This study develops a dual-emission Eu-MOF ratiometric fluorescence sensor for biogenic amine detection in food spoilage monitoring. The sensor exhibits dual emission at 495 nm (ligand π→π* transition) and 615 nm (ligand-to-metal charge transfer, LMCT) through the antenna effect. Biogenic amines specifically regulate the intensity ratio (I/I) by adsorbing into MOF pores via hydrogen bonding, triggering a dynamic equilibrium switch between LMCT and ligand-to-ligand charge transfer (LLCT), causing fluorescence color shift from orange to green.

Morphological map of under- and overexpression of genes in human cells.

Cell Painting images offer valuable insights into a cell's state and enable many biological applications, but publicly available arrayed datasets only include hundreds of genes perturbed. The JUMP Cell Painting Consortium perturbed roughly 75% of the protein-coding genome in human U-2 OS cells, generating a rich resource of single-cell images and extracted features. These profiles capture the phenotypic impacts of perturbing 15,243 human genes, including overexpressing 12,609 genes (using open reading frames) and knocking out 7,975 genes (using CRISPR-Cas9).

Engineering stable cd-based metal-organic framework photocatalysts via ligand pre-metallization and polynuclear clusters: enhanced charge separation for ciprofloxacin degradation.

To address environmental remediation challenges from ciprofloxacin (CIP), this study pioneers a ligand pre-metallization strategy diverging fundamentally from conventional post-synthetic approaches. By pre-constructing Fe-N coordination in Fe(III) meso-tetra(4-carboxyphenyl)porphine chloride (HTCPP(Fe)) ligands before assembly with Cd polynuclear clusters, a stable three-dimensional Fe-doped Cd-TCPP metal-organic framework (MOF) was engineered, overcoming pore collapse limitations in conventional porphyrinic MOFs. This coordination engineering enables triple synergistic mechanisms: (1) Metal-to-ligand charge transfer (MLCT)-enhanced light absorption, (2) built-in electric fields at FeN-Cd heterojunctions reducing charge-transfer resistance, and (3) high-density active sites accelerating hydroxyl radical (OH) production.

Trends and Future Projections of Vitreoretinal Surgery in a Tertiary Hospital in China (2005-2022).

Purpose: To analyze past trends and project future trajectories of vitreoretinal surgery in China.

Methods: A single-institution, retrospective analysis of vitreoretinal surgeries conducted at Zhongshan ophthalmic center (ZOC), Sun Yat-sen University, from 2005 to 2022, utilizing ZOC's inpatient database. Patients hospitalized in the retinal surgery department were included.

Near infrared fluorescence probe for dynamic and reversible monitoring of redox homeostasis fluctuations of hemangioma.

Intracellular redox homeostasis is indispensable for the physiological processes of organisms and represents a pivotal mechanism underlying drug therapy for tumors. Among the various redox conjugates, superoxide anion (O) and glutathione (GSH) play crucial roles in reflecting the redox state of living cells. Given the significance of the redox pair between O and GSH in living organisms, herein, we report a near-infrared (NIR) fluorescent probe, BDPos, that enables real-time visualization of propranolol-induced redox imbalance during vascular tumor therapy by monitoring the dynamics alterations of O and GSH.

Association between ultrasound-based biliary and parenchymal intrahepatic mass-forming cholangiocarcinoma subtypes and clinicopathological features and survival.

Objective: Mass-forming intrahepatic cholangiocarcinomas (MF-ICCs) can be classified into ductal and parenchymal types using magnetic resonance imaging (MRI). We aimed to subclassify MF-ICC into biliary and parenchymal types based on ultrasound (US) findings and to investigate the differences in their contrast-enhanced ultrasound (CEUS) patterns, clinicopathologic features, and prognosis.

Methods: In this study, 141 patients who underwent US with pathologically proven MF-ICC from two hospitals were retrospectively enrolled.

Evaluation of the risk factors for rhegmatogenous retinal detachment associated with choroidal detachment from the viewpoint of treatment patterns: a retrospective study.

Aim: To compare the proportion of rhegmatogenous retinal detachment (RRD) associated with choroidal detachment (RRDCD) in the emergency surgery group with the routine inpatient surgery group and determine risk factors for RRDCD.

Methods: A total of 694 patients (694 eyes) diagnosed with RRD in the emergency surgery (the median duration of RRD was 5d) group were included from the Department of Ophthalmic Emergency, and 692 patients (eyes) in the routine inpatient surgery group (the median duration was 15d) were selected randomly from the Ocular Fundus Department. Demographics, refractive status, macular status, lens status, extent of retinal detachment, number of retinal breaks, duration of symptoms before surgery, and the incidence of RRDCD were compared.

Prenatal exposure to PM constituents and newborn leukocyte telomere length in a prospective study: Windows of susceptibility and modification by maternal folic acid supplementation.

Prenatal exposure to fine particulate matter (PM) is related to alterations in newborn telomere length (TL), an indicator of cellular aging. However, the specific effects of PM constituents and windows of susceptibility are unknown. We aimed to identify the susceptibility windows for prenatal PM constituents affecting newborn leukocyte TL (LTL) and examine the modifying role of folic acid (FA) supplementation.

Diagnosis of Sarcopenia Using Convolutional Neural Network Models Based on Muscle Ultrasound Images: Prospective Multicenter Study.

Background: Early detection is clinically crucial for the strategic handling of sarcopenia, yet the screening process, which includes assessments of muscle mass, strength, and function, remains complex and difficult to access.

Objective: This study aims to develop a convolutional neural network model based on ultrasound images to simplify the diagnostic process and promote its accessibility.

Methods: This study prospectively evaluated 357 participants (101 with sarcopenia and 256 without sarcopenia) for training, encompassing three types of data: muscle ultrasound images, clinical information, and laboratory information.

Association between dietary oxidative balance scores and myocardial infarction in diabetic patients: insights from NHANES 1999-2018.

Background: Myocardial infarction (MI) poses a serious health threat to diabetic patients, who are particularly vulnerable due to heightened oxidative stress. The dietary oxidative balance score (DOBS) quantifies the overall oxidative profile of the diet and may reflect diet-related cardiovascular risk.

Objective: This study aimed to evaluate the association between DOBS and the risk of MI among diabetic individuals using a nationally representative U.

Preoperative identification of hepatocellular carcinoma from focal liver lesions ≤ 20 mm in high-risk patients using clinical and contrast-enhanced ultrasound features.

Objective: We aimed to develop and validate a prediction model to identify HCC in focal liver lesions (FLLs) ≤20 mm among patients at risk for HCC based on clinical and contrast-enhanced ultrasound (CEUS) features.

Methods: Between January 2022 and July 2023, 386 patients (mean age 58 ± 11 years; 277 male) at risk for HCC with FLLs ≤20 mm and clinical and preoperative CEUS data from three centers were retrospectively enrolled. Three prediction models based on clinical data (Cli-M), CEUS features (CEUS-M), and combined clinical and CEUS features (Com-M) were constructed using the training cohort (187 patients).

Advances in polysaccharide-based antibacterial materials.

Microbial contamination is a major threat to the public health and a primary cause of food spoilage, leading to significant economic losses worldwide. Various materials have been used to combat microbes, including inorganic materials, metals and polymers. Among these, natural polymers have attracted much attention in both academic and industrial research due to their abundance, renewability, biocompatibility, biodegradability and ease of processing.

One-Month Silicone Oil Tamponade Can Decrease Intraocular Complications via Reducing the Recruitment and Activation of Leukocytes in Patients with Rhegmatogenous Retinal Detachment.

Introduction: The aim of the study was to compare the effects of 1-month versus routine-duration silicone oil (SO) tamponade on intraocular inflammatory cytokine levels and clinical outcomes in patients undergoing vitrectomy for rhegmatogenous retinal detachment.

Methods: A prospective study from May 2019 to March 2022 divided patients undergoing vitrectomy into routine and early groups. Aqueous humor was sampled before SO removal to measure cytokine levels, including granulocyte-macrophage colony-stimulating factor, interferon (IFN)-γ, interleukin (IL)-4, IL-8, IL-10, IL-13, IL-17, macrophage inflammatory protein (MIP)-1α, tumor necrosis factor (TNF)-α, and IL-1α.

TREM2 Modulates Postoperative Cognitive Function in Aged Mice by Inhibiting the NLRP3/caspase-1 Pathway and Apoptosis via PLCγ2 Activation.

Postoperative cognitive dysfunction (POCD) is a prevalent complication in elderly patients, with neuroinflammation identified as a key contributing factor. This study investigates the therapeutic potential of the TREM2-PLCγ2 signaling pathway in mitigating neuroinflammation, neuronal apoptosis and cognitive impairment following surgery. We employed both in vivo and in vitro models to investigate the effects of TREM2 activation and its interaction with PLCγ2.

Microglial repopulation alleviates surgery-induced neuroinflammation and cognitive impairment in a ZEB1-dependent manner.

Microglia play a crucial role in postoperative cognitive dysfunction (POCD). This study investigated the effects of microglial depletion and subsequent repopulation on POCD and its underlying mechanisms. An aged mouse model of POCD was induced by partial hepatectomy, and the colony-stimulating factor 1 receptor (CSF1R) inhibitor PLX5622 was administered to facilitate microglial depletion and repopulation.

MSCs act as biopatches for blood-retinal barrier preservation to enhance functional recovery after retinal I/R.

Retinal ischemia/reperfusion (I/R) is one of the most common pathologies of many vision-threatening diseases and is caused by blood-retinal barrier (BRB) breakdown and the resulting inflammatory infiltration. Targeting BRB is promising for retinal I/R treatment. Mesenchymal stromal cells (MSCs) are emerging as novel therapeutic strategies.

Identification of SNPs in the second intron of and their Association with growth traits in Nanjiang Yellow goat.

Insulin-like Growth Factor 2 mRNA-binding Protein 1 () is a candidate gene of significant interest for modulating economically important traits in livestock and poultry. The second intron of has been implicated in growth-related traits, though its precise mechanistic role remains elusive. Initial resequencing analyses in our laboratory indicated strong selective pressures on the genomic region, prompting the selection and identification of several single nucleotide polymorphisms (SNPs).

Transdermal microneedle-assisted ultrasound-enhanced CRISPRa system to enable sono-gene therapy for obesity.

Obesity, a surging global health challenge, necessitates effective, accessible and innovative therapeutic models. Here we develop a spatiotemporally controllable microneedle (MN) drug delivery platform for sono-gene therapy to fight obesity. The platform delivers the methoxy polyethylene glycol-polyethyleneimine (mPEG-PEI) modified metal-organic frameworks (MOFs) sonosensitizer and the clustered regularly interspaced short palindromic repeats-activating (CRISPRa)/CRISPRa-uncoupling protein 1 (UCP1) system intradermally to adipocytes.

Exploring the mechanism of SLXG for treating nonalcoholic fatty liver disease based on network pharmacology and molecular docking.

Background: The Shugan Lidan Decoction and Chaihu Shugan formula are traditional Chinese medicine formulas for treating liver diseases, with a history of over a 1000 years. By comprehensively improving 2 traditional Chinese medicinal formulas, Shugan Lidan Xiaoshi Granules (SLXG) has been developed for the treatment of nonalcoholic fatty liver disease (NAFLD) and other liver-related metabolic diseases.

Methods: First, the effective active ingredients and targets of SLXG were determined using the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform database.

The value of ultrasound-measured rectus femoris thickness, cross-sectional area, and shear wave velocity in assessment of muscle in postmenopausal women with osteosarcopenia.

Objectives: To evaluate the value of ultrasound (US) and shear wave velocity (SWV) to assess muscle in postmenopausal women with osteosarcopenia (OSP).

Methods: This study included 145 postmenopausal women, comprising 115 osteopenia/osteoporosis participants without sarcopenia (OP alone) and 30 OSP participants. All received the evaluation of bone mineral density (BMD), appendicular skeletal muscle mass index (ASMI), handgrip strength, calf circumference, 6-meter walking speed, and 5-time chair stand test.

Preoperative dapagliflozin use and cardiac surgery-associated acute kidney injury: A single-center retrospective cohort study.

Background: No drug has been shown to be effective in preventing cardiac surgery-associated acute kidney injury (CSA-AKI). In different clinical settings, sodium-glucose transporter 2 (SGLT2) inhibitors confer renal protection and may be promising drug candidates. We examined the association between preoperative dapagliflozin use and the incidence and prognosis of CSA-AKI.

Design of a Co doped carbon Backbone with self-grown Au nanoparticles via a 'Triple Advantage' Strategy for sensitive dopamine detection.

In this study, a Co doped polyhedral carbon skeleton (Co CN) was prepared by nitrogen carbonization using ZIF-67 as a precursor. The Co CN features a rough surface with excellent electrical conductivity, and the Co atoms exhibit unique catalytic properties. Based on these characteristics, we used Co CN as a carrier to load Au nanoparticles (NPs) onto its surface through the linkage and reduction effects of polyoxometalates (POMs).

Microwave ablation for benign thyroid nodules with cosmetic problems and related factors for post-ablative complete relief: a two-center retrospective study.

Purpose: To investigate the efficacy of ultrasound (US)-guided percutaneous MWA for benign thyroid nodules with cosmetic problems and identify related factors contributing to post-ablative complete relief (CR).

Methods: This retrospective study reviewed the efficacy of MWA in treating thyroid nodules in patients with cosmetic problems who underwent MWA from January 2021 to May 2023. Patients were followed up at 1, 3, and 6 months after MWA.

Novel N-doped biomass carbon spheres loaded with In-Situ grown FeCo layered double hydroxide for oxygen evolution reaction.

To improve the efficiency of the oxygen evolution reaction (OER) while minimizing energy consumption and costs, we propose a novel design strategy. Oatmeal, an abundant and inexpensive feedstock rich in carbon (C), nitrogen (N), and oxygen (O), serves as an ideal precursor for biochar synthesis. By utilizing N-doped carbon spheres (NCS) as carriers, we achieved uniform growth of ZIF-67 on their surface through polyvinyl pyrrolidone (PVP) activation.