ABCC3 mediates insecticide metabolism and functions as a receptor for Bt toxins in Plutella xylostella.

Plutella xylostella is a major global pest with increasing resistance to insecticides. ATP-binding cassette (ABC) transporters are implicated in the metabolism of insecticides, but their molecular mechanisms remain poorly understood. Here, we identified and characterized the role of P.

Glucose-Responsive Self-Rolling Antioxidant Hydrogel Actuators for a Cell Culture Platform.

Three-dimensional (3D) cell culture technology can mimic the physiological characteristics of tissues and organs, making it highly suitable for cell therapy, organ chips, and tissue engineering applications. However, achieving a uniform cell distribution within the 3D matrix while mitigating the effects of reactive oxygen species (ROS) accumulation generated during the 3D cell culture remains a critical challenge. Hydrogel actuators, with their excellent bioactivity and controllable self-rolling behavior, provide an optimal microenvironment for the 3D cell culture.

Enhanced Production of Santalols by Engineering the Cytochrome P450 Enzyme to the Peroxisomal Surface in Yeast.

Cytochrome P450 enzymes are crucial for producing complex plant natural products (PNPs), but their expression and activity in heterologous systems are often limited. This study explores subcellular engineering to improve PNPs production by synthesizing (Z)-santalols─high-value components of sandalwood oil─in GRAS (Generally Recognized As Safe) . By directing the mevalonate pathway and sandalwood oil biosynthesis enzymes to peroxisomes using the ePTS1 peptide, we increased total santalene and (Z)-santalol titer by 5.

Facilitators and barriers to implementing a social franchising model of delivering eye care services in rural China: a qualitative study using the updated Consolidated Framework for Implementation Research.

Objectives: To identify the barriers and facilitators to implementing the social franchising model '' for delivering eye care services to children in rural China, using the updated Consolidated Framework for Implementation Research (CFIR 2.0) as a theoretical basis to evaluate implementation performance drivers and propose strategies for effective scaling and sustainability.

Design: This qualitative study involved semi-structured interviews with key stakeholders from both the supply side and demand side.

Survival benefit of adjuvant chemotherapy and individualized prognosis in resected cHCC-CCA.

Combined hepatocellular-cholangiocarcinoma (cHCC-CCA) is a rare malignancy with poor prognosis and unclear benefit from adjuvant chemotherapy. To identify the appropriate candidates for postoperative adjuvant chemotherapy in cHCC-CCA, we developed a prognostic model to predict patient outcomes and stratify populations accordingly. This retrospective study included 75 cHCC-CCA patients treated at Tianjin Medical University Cancer Institute and Hospital from 2009 to 2019.

Growth and Characterization of n-Type Hexagonal TaO:W Films on Sapphire Substrates by MOCVD.

Tantalum oxide is a wide bandgap material commonly used as an insulating dielectric layer for devices. In this work, hexagonal TaO (-TaO) films doped with tungsten (W) were deposited on -AlO (0001) by metal-organic chemical vapor deposition (MOCVD). The effects of W doping on the structural, morphology, and photoelectrical properties of the obtained films were studied.

Self-reported vision impairment and depressive symptoms among older adults: a longitudinal mediation analysis.

Background: Though vision impairment (VI) is a strong predictor for late-life depression, the underlying mechanisms remain unclear, particularly in low-income and middle-income countries. This study aims to investigate the contribution of underlying pathways in mediating the association between self-reported VI and depression among Chinese older adults.

Methods: We included three waves of data from the China Health and Retirement Longitudinal Study.

Multiplex metabolic engineering of yeast for high-efficiency all-trans-retinoic acid production.

Background: All-trans-retinoic acid (ATRA) is a pivotal signaling molecule with multifaceted roles in cellular regulation and has broad valuable applications in pharmacology and dermatology. The demand for ATRA is growing, but there is a significant gap in research on its effective biosynthesis.

Results: In this study, a β-carotene-producing chassis strain was firstly constructed by mining optimal gene combinations, changing platform strains and adjusting the gene copy numbers.

Epidemiological features of nontyphoidal Salmonella infections reported to foodborne disease surveillance system in china, 2013-2022.

Background: Nontyphoidal Salmonella (NTS) is the most commonly reported foodborne gastrointestinal infection and ranks among the top three causes of foodborne bacterial outbreaks in China.

Methods: This study analyzed NTS data from the Foodborne Disease Surveillance System in China from 2013 to 2022 to summarize epidemiological features and assess reporting rate changes. We employed joinpoint regression model to calculate the annual percentage change in NTS reporting rate, sex reporting rate, as well as rural-urban reporting rate.

Adverse Effects of Pesticides on the Ovary: Evidence from Epidemiological and Toxicological Studies.

Pesticides are widely used in agriculture, public health, and residences to control pests and insects. The safety of exposure to pesticides has raised concerns due to their presence in the environment and their potential effects on human health. The ovary is the major female reproductive organ and is considered a potential target organ for pesticide toxicity.

Individualized Prediction of Postoperative Survival in Gallbladder Cancer: A Nomogram Based on SEER Data and External Validation.

Background: Gallbladder cancer (GBC) is a rare but aggressive malignancy. Prognostic tools are essential for optimizing postoperative treatment strategies. We aim to develop and validate a prognostic nomogram to estimate 1-, 3-, and 5-year overall survival (OS) in GBC patients and explore the role of adjuvant chemotherapy across different subgroups.

The relationship between trait anxiety and sleep quality in college students: an exploratory analysis of physical activity as a moderator.

Objective: This study aimed to examine the association between trait anxiety and sleep quality among college students and to assess whether different levels and components of physical activity (PA) moderate this relationship.

Methods: A cross-sectional survey was conducted among 2,902 college students. Standardized instruments were used to evaluate trait anxiety, sleep quality, and PA levels.

A Systematic Review of the Effects of Repetitive Transcranial Magnetic Stimulation Combined With Pharmacological Intervention on Sleep Quality and Depressive Symptoms in Patients With Depression.

To systematically evaluate the intervention effect of repetitive transcranial magnetic stimulation (rTMS) combined with pharmacological interventions on sleep quality and depressive symptoms in patients with depression, identify optimal stimulation parameters, and explore potential clinical applications to facilitate individualised therapeutic strategies. Databases including Pubmed, Web of Science, Cochrane Library, Embase, CNKI, Wanfang Medical Database, and VIP database were searched up to 31 December 2024, for randomized controlled trials (RCTs) assessing rTMS combined with pharmacological intervention for sleep disorders and depressive symptoms in patients with depression. Data analysis was performed using Stata software.

Is Sociability Generally Linked to Infection? A Study Based on Rhesus and Japanese Macaques.

Parasite infection is one key risk inherent in group living and is considered to influence the formation and evolution of animal societies. Previous studies investigating the relationship between sociability (a measure of an individual's level of social engagement) and parasite infection have yielded mixed results, with some observing positive relationships between social network centrality and infection and others observing negative or no sociability-infection links. Here, we aggregated behavioral and parasitological data from three groups of rhesus macaques (Macaca mulatta brevicaudus) in China and two groups of Japanese macaques (Macaca fuscata and Macaca fuscata yakui) to test whether sociability generally predicts geohelminth infection in macaques with similar social structure.

Molecular insights and treatment innovations: Advancing outcomes in acute myeloid leukemia with myelodysplasia‑related changes (Review).

Acute myeloid leukemia, myelodysplasia‑related (AML‑MR), a challenging and aggressive subtype of AML, is characterized by unique genetic abnormalities and molecular features, which contribute to its poor prognosis compared with other AML subtypes. The present review summarizes the current understanding of AML‑MR pathogenesis, highlighting notable advancements in genetic and cytogenetic insights. Critical mutations, such as those in the tumor antigen p53 and additional sex combs like 1 genes, and their role in disease progression and resistance to treatment, are explored.

Knowledge, attitudes, and practices regarding food-medicine dual-purpose substances among adults in China: a cross-sectional online survey.

Background: Food medicine homology originated in ancient China and has been officially named food-medicine dual-purpose substances (FMS). However, the relationship between residents' knowledge, attitudes, practices (KAP) and FMS among adults in China remains unclear. This study evaluated the current situation of FMS-related knowledge, awareness and actual consumption behaviors and practices of Chinese residents to provide a basis for both perfect related regulations and scientific advice on FMS consumption.

Diarrheagenic Outbreak Reporting to Foodborne Disease Outbreaks Surveillance System - China, 2011-2022.

What Is Already Known About This Topic?: Foodborne diarrheagenic (DEC) outbreaks constitute a significant global public health concern, yet comprehensive data on outbreak incidence and epidemiological characteristics in China remain limited.

What Is Added By This Report?: Between 2011 and 2022, there were 413 foodborne DEC outbreaks reported to foodborne disease outbreak surveillance system, resulting in 8,127 illnesses, 2,565 hospitalizations, and one fatality. Enteroaggregative (EAEC) emerged as the predominant causative pathogen (48.

Epidemiological Characteristics of Sporadic Foodborne Diseases Caused by - China, 2013-2022.

Introduction: () is a common foodborne pathogen in coastal areas of China. Most epidemiological studies on have focused on foodborne disease outbreaks, with fewer studies based on long-term, continuous, and systematic sentinel surveillance.

Methods: Data were collected from the national foodborne disease active surveillance program in 31 provincial-level administrative divisions (PLADs) in China from 2013 to 2022.

The impact of neoadjuvant therapy on the prognostic value of preoperative neutrophil-to-lymphocyte ratio for colorectal liver metastases: a multi-center cohort study.

Background: Neutrophil-to-lymphocyte ratio (NLR) is a promising prognostic marker for patients undergoing hepatectomy for colorectal liver metastases (CRLM). However, its prognostic value in patients receiving neoadjuvant therapy (NAT) has not been sufficiently addressed.

Methods: From 2013 to 2023, a cohort of 692 patients with CRLM receiving hepatectomy were enrolled in five centers.

PIKFYVE deficiency induces vacuole-like cataract via perturbing late endosome homeostasis.

Phosphoinositide kinase, FYVE-type zinc finger containing (PIKFYVE) was recently identified as a causative gene for cataract. Pikfyve phosphatidylinositol phosphate kinase domain-deficient (pikfyve) zebrafish lens and PIKFYVE-inhibited human lens epithelial cells developed vacuoles, colocalized with late endosome marker RAB7. In this study, the pikfyvezebrafish with vacuole-like cataract underwent transcriptomic and proteomic analyses to explore the underlying mechanisms of vacuole formation.

Morbidity, Mortality, and Epidemiological Features of Clinical Invasive Listeriosis Isolates from Sentinel Surveillance in Beijing, China, 2013-2022.

Listeriosis is a relatively rare but severe foodborne disease, which has significant public health concern of persons with underlying conditions and pregnant women. This study aimed to estimate the morbidity, mortality, and fatality rates of listeriosis over a 10-year period and clarify the epidemiological features of the pathogen in Beijing, China, based on voluntary reporting of sentinel surveillance. A total of 228 listeriosis cases were reported with annual morbidity rate of 1.

A Listeriosis Case Associated with Ice Cream Consumption in China in 2019.

During the 2019 Chinese National Microbiological Food Safety Surveillance program, was detected in ice cream samples produced by Manufacturer A. By comparing the sequences of isolates derived from the ice cream with those of isolates derived from humans, we identified one human-derived isolate that was genetically indistinguishable from one ice cream-derived isolates. The patient was hospitalized with listeriosis in Beijing, China in March 2019.

GOLM1 promotes cholesterol gallstone formation via ABCG5-mediated cholesterol efflux in metabolic dysfunction-associated steatohepatitis livers.

Background/aims: Metabolic dysfunction-associated steatohepatitis (MASH) is a significant risk factor for gallstone formation, but mechanisms underlying MASH-related gallstone formation remain unclear. Golgi membrane protein 1 (GOLM1) participates in hepatic cholesterol metabolism and is upregulated in MASH. Here, we aimed to explore the role of GOLM1 in MASH-related gallstone formation.

Illuminating RNA through fluorescent light-up RNA aptamers.

Visualizing RNA is critical for understanding RNA expression patterns and spatial organization within cells, offering valuable insights into gene regulation and cellular functions. High-resolution RNA imaging techniques are therefore indispensable for revealing the complexities of cellular pathways and physiological processes. Traditional RNA imaging methods, however, face significant limitations, such as high background noise resulting from labeling or cell fixation, which can impede the accurate tracking of RNA dynamics in live cells.

A Multifunctional Nanodrug Co-Delivering VEGF-siRNA and Dexamethasone for Synergistic Therapy in Ocular Neovascular Diseases.

Introduction: Oxidant stress, abnormal angiogenesis, and inflammation are three key factors contributing to the development of ocular neovascular diseases (ONDs). This study aims to develop a multifunctional nanodrug, DEX@MPDA-Arg@Si (DMAS), which integrates mesoporous polydopamine, vascular endothelial growth factor (VEGF)-siRNA, and dexamethasone (DEX) to address these therapeutic targets.

Methods: Physicochemical properties of DMAS were measured using transmission electron microscopy and a nanoparticle size analyzer.