Coordinated regulation of pH alkalinization by two transcription factors promotes fungal commensalism and pathogenicity.

As a clinically relevant opportunistic human fungal pathogen, Candida albicans is able to rapidly sense and adapt to changing microenvironments within the host, a process that is essential for its successful invasion and survival. Although studies have shown that the transcription factor Stp2 is the master regulator of environmental alkalinization, accumulating evidence supports a clear involvement of other participants in this adaptation process. Here, following a large-scale genetic screen, we identify the transcription factor Dal81 as an uncharacterized positive regulator of pH alkalinization in C.

Bufei Jiedu Formula enhances CD40 activation and macrophage polarization to eliminate intracellular MRSA persisters.

Background: Intracellular methicillin-resistant (MRSA) represents a complex infection in clinical practice, characterized by its refractory and recurrent nature, rendering it challenging to treat with conventional antibiotics. Bufei Jiedu Formula (BFJD) is a traditional Chinese medicine compound utilized for treating chronic lung infections; however, its mechanisms against intracellular MRSA infection are not yet fully understood.

Methods: An animal model with persistent MRSA infection was used to evaluate the efficacy of BFJD against chronic bacterial infections.

Oxytropis falcata bunge extract combined with black soybean oil ameliorates DNCB-induced atopic dermatitis-like skin inflammation.

Background: Bunge (OF) is a traditional Tibetan medicine, while black soybean oil (BSO) is a contemporary treatment used for eczema. Pharmacological studies have indicated that both exhibit strong anti-inflammatory effects. However, the role of OF in atopic dermatitis remains uncertain.

Preparation and evaluation of oral enteric sustained-release liquid formulations of aspirin.

Aim: To develop an enteric sustained-release nanoparticle formulations of aspirin for applicability to a broader population.

Methods: Aspirin-loaded nanoparticles were prepared using poly(lactic-co-glycolic acid) (PLGA) and the enteric polymer Eudragit L100-55 through an optimized oil-in-water emulsification solvent evaporation method. The nanoparticles were subjected to comprehensive physicochemical characterization, including particle size, zeta potential, and morphological analysis.

A dual-peptides and specific promoter-modified nano gene delivery system for myocardial hypertrophy treatment.

Cardiac hypertrophy represents the heart's adaptive response to physiological or pathological stimuli, functioning to alleviate ventricular wall stress and preserve cardiac function and efficiency. However, pathological hypertrophy usually progresses to heart failure. Gene therapy, in contrast to conventional chemotherapeutic drugs, has the ability to impact cardiac hypertrophy directly, while the lack of adequate vectors limits its application.

Ilizarov technique for treatment of a giant aneurysmal bone cyst at the distal femur: A case report.

Background: Aneurysmal bone cyst (ABC) is a benign cystic of unknown etiology, characterized by multiple chambers and a high recurrence rate. Current treatment options include vascular embolization, surgical excision, curettage with cavity filling, sclerosing agent injection into the cavity, radiotherapy, and systemic drug therapy. Among these, surgical excision and curettage are the preferred treatment modalities.

Construction and evaluation of liposomal drug delivery system for an ALK/HDACs dual-targeted inhibitor with sustained release and enhanced antitumor effect.

ALK/HDACs dual target inhibitor (PT-54) was a 2,4-pyrimidinediamine derivative synthesized based on the pharmacophore merged strategy that inhibits both anaplastic lymphoma kinase (ALK) and histone deacetylases (HDACs), which has demonstrated significant efficacy in treating multiple cancers. However, its poor solubility in water limited its clinical application. In this study, we prepared PT-54 liposomes (PT-54-LPs) by the membrane hydration method to overcome this defect.

Compact and water flushing resistant mesh biofilms forming at short SRT disappeared naturally under extended SRT in dynamic membrane bioreactor.

Extending the solids retention time (SRT) has been demonstrated to mitigate membrane biofouling. Nevertheless, it remains an intriguing question whether the compact and water flushing resistant mesh biofilms developed at short SRT can undergo biodegradation and be removed with extended SRT. In present study, the bio-fouled mesh filter in the 10d-SRT dynamic membrane bioreactor (DMBR), with mesh surfaces and pores covered by compact and water flushing resistant biofilms exhibiting low water permeability, was reused in the 40d-SRT DMBR without any cleanings.

Single-cell transcriptome analysis reveals keratinocyte subpopulations contributing to psoriasis in corneum and granular layer.

Background: Psoriasis is a chronic, inflammatory skin disease that is common and relapses easily. While the importance of keratinocyte proliferation in psoriasis development is well-documented, the specific functional subpopulations of epidermal keratinocytes associated with this disease remain enigmatic.

Materials And Methods: Therefore, in our analysis of single-cell transcriptome data from both normal and psoriatic skin tissues, we observed significant increases in certain keratinocytes in the stratum corneum (KC) and stratum granulosum (KG) within psoriatic skin.

The regulatory subunits of CK2 complex mediate DNA damage response and virulence in Candida Glabrata.

Background: Candida glabrata which belongs to normal microbiota, has caused significant concern worldwide due to its high prevalence and drug resistance in recent years. C. glabrata has developed many strategies to evade the clearance of the host immune system, thereby causing persistent infection.

Correlation analysis between CYP2C9 polymorphisms and liver injury induced by flurbiprofen axetil in 100 Han Chinese patients.

This study explored correlation between CYP2C9 polymorphisms and liver injury induced by flurbiprofen axetil (FBA). A total of 100 patients undergoing primary total knee arthroplasty (TKA) were mainly administered with basic analgesic, FBA. All the patients participating in this study were required to take blood samples for detecting CYP2C9 polymorphisms before surgery after admission.

Supplementation of hyaluronic acid injections with vitamin D improve knee function by attenuating synovial fluid oxidative stress in osteoarthritis patients with vitamin D insufficiency.

Objectives: There is still controversy about the effect of vitamin D supplementation on osteoarthritis (OA). The purpose of this study was to investigate the effects of vitamin D supplementation with Hyaluronic acid (HA) injection on OA.

Methods: We investigated serum vitamin D levels and oxidative stress (OS) in synovial fluid from patients with OA who underwent total knee arthroplasty (grade IV, = 24) and HA injection (grade II and III, = 40).

On the Investigation of Frequency Characteristics of a Novel Inductive Debris Sensor.

Lubricants have the ability to reduce frictions, prevent wear, convey metal debris particles and increase the efficiency of heat transfer; therefore, they have been widely used in mechanical systems. To assess the safety and reliability of the machine under operational conditions, the development of inductive debris sensors for the online monitoring of debris particles in lubricants has received more attention from researchers. To achieve a high-precision, high-efficiency sensor for accurate prediction on the degree of wear, the equivalent circuit model of the sensor coil has been established, and its equations discovering the relationship between the induced voltage and excitation frequency have been derived.

Analgesia with reduced incidence of adverse reactions using flurbiprofen axetil in combination with half standard-dose opioids in primary total knee arthroplasty.

Objectives: To determine the analgesic effect of flurbiprofen axetil (FBA) combined with half standard-dose opioids in patients undergoing primary unilateral total knee arthroplasty (TKA).

Materials And Methods: A total of 100 patients undergoing primary TKA were randomly divided into two groups, namely a control group and an experimental group, with 50 patients in each group. All patients received the same dose of FBA in the form of a patient-controlled intravenous analgesia but in the control group this was combined with a standard-dose of opioids and in the experimental group with a half standard-dose of opioids.

Research on safety early warning of uranium tailings dam based on abnormal radioactive indexes of water leachate.

The radioactive index value of the leachate of the uranium tailings dam is affected by the internal damage of the dam. Therefore, a way of using the deviation of the radioactive index concentration in the leachate to warn the instability of the dam is innovatively proposed in this paper. Firstly, the SSA-BP algorithm is used to predict and analyze the five groups of parameters U, Ra, ∑ α, ∑ β and Rn.

Elucidation of the underlying mechanism of Hua-ban decoction in alleviating acute lung injury by an integrative approach of network pharmacology and experimental verification.

The pathogenic hyper-inflammatory response has been regarded as the major cause of the severity and death related to acute lung injury (ALI). Hua-ban decoction (HBD) is a classical prescription in traditional Chinese medicine (TCM). It has been extensively used to treat inflammatory diseases; however, its bioactive components and therapeutic mechanisms remain unclear.

Alpha-Hemolysin from Staphylococcus aureus Obstructs Yeast-Hyphae Switching and Diminishes Pathogenicity in Candida albicans.

The use of antibiotics can disrupt the body's natural balance and increase the susteptibility of patients towards fungal infections. Candida albicans is a dimorphic opportunistic fungal pathogen with niches similar to those of bacteria. Our aim was to study the interaction between this pathogen and bacteria to facilitate the control of C.

Intraoperative method of femoral head central measurement to prevent leg length discrepancy following hemiarthroplasty.

Purpose: This study aimed to introduce and investigate the safety and efficiency of the intraoperative central measurement method of the femoral head (IM-CMFH) to prevent leg length discrepancies (LLD) after hemiarthroplasty.

Methods: Overall, 79 patients aged 75 to 85 years with femoral neck fractures who underwent hemiarthroplasty were divided into two groups: the Control group ( = 46) and the IM-CMFH group ( = 33). The two groups were compared for postoperative LLD and the proportions of patients with greater than 10 mm, 6-10 mm, and within 5 mm, postoperative femoral offset (FO) difference and the proportions of patients within 5 mm, incremental greater than 5 mm and reduction greater than 5 mm.

The causal relationship between obesity and skin and soft tissue infections: A two-sample Mendelian randomization study.

Objective: Many observational studies have shown that obesity strongly affects skin and soft tissue infections (SSTIs). However, whether a causal genetic relationship exists between obesity and SSTIs is unclear.

Methods: A two-sample Mendelian randomization (MR) study was used to explore whether obesity is causally associated with SSTIs using a publicly released genome-wide association study (GWAS).

Technical note: A method to evaluate the effect of scanning beam delivery error on 3D dose and its utilization on carbon ion radiotherapy for prostate cancer.

Purpose: To establish a method for evaluating the effect of scanning ion beam delivery error on three-dimensional (3D) dose reconstructed on patients' CT based on log file.

Materials And Methods: This study used the MATLAB program to reconstruct the 3D dose on the patient's CT based on the log file (Dose ) for treatment delivery accuracy check. In addition, differences between the parameters in the log file and the treatment plan, such as the spot position, spot size, and particle number, were analyzed, as well as their effects on the dose distribution.