Spatiotemporal coordination of reovirus peripheral core replication to perinuclear whole virus assembly.

Reoviruses coordinate their replication and assembly through intricate spatial and temporal compartmentalization within host cells. In this study, we elucidate the dynamics of mammalian orthoreovirus (reovirus) core replication and viral particle assembly. Using high-resolution immunofluorescence confocal microscopy, we tracked input cores and de novo cores, revealing that input cores initially form peripheral, OC-negative factories that migrate inward while seeding independent peripheral factories.

Multiple Template Site Nitrogen Atom Deletions from Rotaxanes, Catenanes, and a Molecular Knot.

We report the deletion of nitrogen atoms from multiple template sites in rotaxanes, catenanes, and a molecular knot. Nitrogen extrusion from secondary amines in the backbone of the interlocked structures is achieved using -diphenylphosphinylhydroxylamine (DPPH), forming carbon-carbon bonds while largely maintaining the integrity of the original mechanical bonding. We find that DPPH gives improved yields (up to 51%) for nitrogen atom deletions from template sites in rotaxanes compared to an anomeric amide nitrogen-deletion reagent and overcomes a major substrate limitation in that, using DPPH, only one of the substituents of the secondary amine in the rotaxane axle needs to be radical-stabilizing.

Revealing the characteristic honey-like aroma formation in honey-scented black tea via molecular sensory analysis.

Honey-scented black tea is highly favored by consumers for its unique flavor; however, the key components and mechanisms underlying its characteristic aroma remain unclear. The aroma characteristics and key components of honey-scented black tea (MXBT), sweet-scented black tea (TXBT), and honey (FM) were elucidated, and an aroma flavor wheel was constructed. β-Damascenone, phenylacetaldehyde, and linalool oxide I were identified as the primary aroma compounds responsible for the honey-like scent of MXBT.

A-DualUD: Source-free unsupervised domain adaptation via anatomical anchor alignment and dual-path uncertainty denoising for cross-modality medical image segmentation.

Background And Objective: Automated medical image segmentation across different imaging modalities, such as MRI and CT scans, plays a crucial role in improving diagnostic accuracy, treatment planning, and surgical navigation. However, significant domain discrepancies across different modalities, stemming from various acquisition protocols and physical principles, pose a significant challenge for automated segmentation algorithms. Unsupervised domain adaptation (UDA) has gained considerable attention for addressing challenges in cross-modality medical image segmentation from different medical instruments, particularly in scenarios where target domain labels are unavailable.

Prognostic value of red cell distribution width-to-albumin ratio in septic patients with malignancies: A retrospective cohort study based on the MIMIC-IV database.

ObjectiveTo investigate the prognostic value of red cell distribution width-to-albumin ratio (RAR) in sepsis patients with malignancies and evaluate its association with in-hospital mortality.MethodsThe retrospective cohort study was conducted using data extracted from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database. Participants were stratified into quartiles (Q1-Q4) based on RAR levels measured within 24 hours of intensive care unit admission.

Aquatic community synchrony dynamics in response to multitrophic regime shifts: Paleoecological insights from a shallow lake.

Ecological synchrony, the coordinated fluctuation of species or communities, is central to ecosystem stability. Yet how synchrony changes during ecological shifts remains poorly understood. This gap is particularly evident in shallow lakes, where transitions from clear, macrophyte-dominated to turbid, algae-dominated state can dramatically alter synchrony patterns, challenging ecosystem resilience.

Vascular dilation function correlates to pregnant outcomes in women with gestational diabetes mellitus.

Background: Gestational diabetes mellitus (GDM) poses significant risks to maternal and fetal health, necessitating a deeper understanding of its pathophysiology and interventions. Vascular endothelial dysfunction plays a crucial role in diabetic vascular complications, but its relationship with GDM outcomes remains unclear.

Study Design: This study included 278 GDM patients categorized into high and low flow-mediated dilation (FMD) groups based on median FMD values.

Evaluation of subclinical myocardial involvement in systemic lupus erythematosus using multiparametric cardiac magnetic resonance imaging.

Purpose: This study aimed to characterize subclinical myocardial involvement and assess the alterations in biventricular cardiac function in patients with systemic lupus erythematosus (SLE) using cardiac magnetic resonance (CMR) imaging.

Method: This study included 42 patients with SLE, categorized into two subgroups: those with subclinical myocardial involvement and a control group with no myocardial involvement. Additionally, 20 age- and sex-matched healthy controls (HCs) underwent CMR examinations.

Low-dose ultrahigh-resolution PCCT enhances subsolid nodule characterization.

Purpose: To characterize invasion-associated CT features in pulmonary subsolid nodules using low-dose ultrahigh-resolution (UHR) photon-counting CT (PCCT) images and evaluate UHR's diagnostic superiority over standard high-resolution (HR) images.

Methods: Patients with subsolid lung adenocarcinoma were recruited for chest scan on PCCT to obtain UHR and standard HR images between November 2023 and May 2024. Nodule characteristics were visually assessed and histogram features were extracted from each nodule.

Eosinophilic gastroenteritis with negative endoscopic biopsy and no peripheral eosinophilia: A case report.

Rationale: Eosinophilic gastroenteritis (EoGE) is a rare inflammatory disease that can affect the entire gastrointestinal tract. Klein et al classified EoGE into 3 distinct subtypes according to the depth of eosinophilic infiltration: mucosal, muscular, and serosal.

Patient Concerns: We herein report a case of a 29-year-old woman who presented with abdominal pain, nausea, and vomiting, with no history of adverse reactions to any allergens.

A Novel C-Terminal Small Tail Provides Thermostability of FeSOD Implying a New Mechanism of Protein Heat Resistance.

Superoxide dismutase (SOD) is found in a variety of organisms, including animals, plants, and microorganisms, and is widely used in medicine, food, and cosmetics. In this study, a novel heat-resistant SOD from Rhodothermus sp. XMH10 (RhSOD) has been found to have no loss of activity at 80 °C and exhibit high thermal stability across a temperature range from 20 °C to 80 °C.

[Current status and challenges of development of acupuncture medical devices of TCM].

The paper summarizes the authorized invention patents, device registration and the relevant published articles of acupuncture medical devices of TCM in recent 5 years, and analyzes the current status and challenges in this field. It is discovered that the optimization and substitution in diagnosis and treatment of acupuncture are involved in the development of acupuncture medical devices. The technology application of these devices are composed of traditional and emerging engineering technologies; and the theoretical guidance for their development requires the integration of traditional acupuncture principles with modern medical theories.

A "mass-forming" variant of ischemic colitis following COVID infection: a case report and review.

Ischemic bowel disease is a common disease of bleeding in the lower digestive tract. There are many risk factors for ischemic bowel disease. Abdominal pain, bloody stool and diarrhea were often the triad of clinical manifestations.

Hydrogen-bonded supramolecular polymer networks for effective removal of perchlorate in water via clustered hydrogen-bonding.

Perchlorate is a toxic, explosive, and water-soluble pollutant but while efficient and low-cost removal of ClO from water is an important challenge, suitable methods for precipitation of ClO from water are underdeveloped. Here, we demonstrate a hydrogen-bonded supramolecular polymer network crystal (HBPC) for efficient complexation of ClO. The HBPC network is constructed by an A,A'-bis-pyridyl-hydrazone-phenyl conjugate-functionalized pillar[5]arene (PYP5), which self-assembles via the formation of clustered hydrogen-bonds.

Photocatalytic Fluorosulfonylation/Fluorosulfonamidation of -Homoallyl Aldehyde Hydrazones to Access FSO-/FSON-Functionalized Tetrahydropyridazines.

Sulfonyl/sulfamoyl fluoride and tetrahydropyridazine cores represent pivotal structural units in chemical and medicinal science. We herein disclose a photocatalytic strategy for the fluorosulfonylation/fluorosulfonamidation of -homoallyl aldehyde hydrazones using FABI (1-fluorosulfonyl-2-arylbenzoimidazolium triflate salts) and NFSAP (-fluorosulfamoylpyridinium salts), enabling efficient access to sulfonyl-/sulfamoyl-substituted tetrahydropyridazines. This protocol demonstrates excellent synthetic scalability and facilitates diverse postderivatization reactions, including SuFEx click reactions and elimination processes.

Enhancing the therapeutic efficacy of gemcitabine in bladder cancer through TGF-β1 inhibition and pluronic F-127-based microsphere delivery.

Bladder cancer is prevalent and features significant recurrence and progression rates, necessitating effective treatment strategies. Gemcitabine, commonly used to treat non-muscle invasive bladder cancer (NMIBC), shows moderate efficacy and notable side effects. TGF-β, not only a key in epithelial-mesenchymal transition(EMT) but also in tumor development, offers a target for enhancing gemcitabine efficacy.

Dual function of heterotrophic ammonia-oxidizing bacteria in facilitating maize compensatory growth under limited rewatering after drought.

Water scarcity threatens global food security, making drought resilience in crops like maize crucial. In response to this challenge, this study investigates the potential of heterotrophic ammonia-oxidizing bacteria (HAOB) to enhance maize compensatory growth under post-drought limited rewatering conditions. Specifically, we focus on the dual mechanism of HAOB in modulating cytokinin synthesis and transport, aiming to develop an innovative agricultural biotechnology to support sustainable crop production.

Triarylboron-Doped Polymer Matrix Ultralong Room-Temperature Phosphorescent Materials for Advanced Multidimensional Information Encryption.

Constructing polymer-based ultralong organic room-temperature phosphorescence (UORTP) materials that exhibit time-dependent phosphorescent color variation holds profound significance. Triarylboranes, as a typical class of fluorophores, are widely used in the fabrication of various functional organic luminescent materials, and achieving UORTP materials from triarylboranes has become an important studied topic. In this study, a polymer-based UORTP material is fabricated by means of doping organic phosphor () composed of boronic acid-modified triarylborane unit into a poly(vinyl alcohol) (PVA) matrix, which is attributed to the hydrogen bonding between and PVA, effectively suppressing the nonradiative decay.

The burden of atrial fibrillation/atrial flutter in Europe from 1990 to 2021, with a forecast of incidence through 2044.

Background: The objective of this analysis was to assess the impact of atrial fibrillation (AF)/atrial flutter (AFL) across various European regions and countries from 1990 to 2021.

Methods: Using the global burden disease 2021 analytical tools, this study evaluated the incidence, prevalence, disability-adjusted life years (DALYs) and death associated with AF/AFL across the European Region, as defined by the World Health Organization, which includes 53 member countries (EU-53), as well as the European Union, as defined in 2019, consisting of 28 member countries (EU-28) from 1990 to 2021.

Results: The EU-53, in 2021, there were 957,812 incident cases [95% uncertainty interval (UI): 773,898 to 1,178,186], 103,043 deaths (95% UI: 86,887 to 111,924), and 2,196,895 DALYs (95% UI: 1,847,967 to 2 596 530) attributed to AF/AFL.

Single-cell sequencing reveals the role of SALL4 in cervical cancer development.

Objective: This study aims to investigate the role of SALL4 in the development and progression of cervical cancer, particularly its impact on the proliferation, migration and adhesion of HeLa cells, and to explore the clinical potential of SALL4 as a therapeutic target.

Methods: Single-cell sequencing technology was utilized to analyze the cellular characteristics of cervical cancer tumor cell populations, and transcriptomic data were integrated to assess the differential expression of SALL4. Additionally, both in vitro and in vivo experiments were conducted to evaluate the effects of SALL4 inhibition on cell proliferation, migration, adhesion, and its regulation of collagen content and fibrosis.

KDM4B enhances immune surveillance via demethylating cGAS.

Cyclic GMP-AMP synthase (cGAS) serves as a crucial sentinel in innate immunity by sensing cytosolic DNA, yet the molecular mechanisms governing its activation remain incompletely understood. Here, we identify lysine demethylase 4B (KDM4B) as the specific demethylase that erases cGAS K350 methylation, facilitating its chromatin release and subsequent activation. Genetic ablation of Kdm4b compromised both antiviral immunity against HSV-1 infection and antitumor responses, while also diminishing the efficacy of anti-PD-1 immunotherapy.

Ultrasonographic characterization and prognostic follow-up of fetal cardiac involvement in maternal immune diseases.

Objective: To analyze the clinical characteristics, echocardiographic features, and prognosis of fetuses with maternal immune diseases.

Methods: We retrospectively evaluated 20 fetuses with cardiac manifestations due to maternal immune diseases from our center between 2019 and 2023 in China. Clinical and echocardiographic data of fetuses and maternal sero-immunity were collected.

The effect of novel solvent formula of primer containing functional monomer on improving the durability of dentin adhesion.

Objective: The study was designed to investigate how varying ratios of a functional monomer, 4-formylphenyl acrylate (FA) primer, formulated with a blend of ethyl-acetate (EAC) and ethanol (ET), impact the adhesive durability of All Bond Universal (ABU) and Single Bond Universal (SBU) under both etching and rinsing modes.

Methods: FA was synthesized via the acyl chloride reaction. FA primer was prepared by mixing ethyl acetate and ethanol as a binary solvent system at solvent ratios of 7:3, 6:4, 5:5, and 4:6.

Preoperative Multifrequency MR Elastography for Stratifying Wnt/β-Catenin Pathway and Microvascular Invasion Phenotypes in Hepatocellular Carcinoma.

Background: The Wnt/β-catenin signaling pathway and microvascular invasion (MVI) are associated with the prognosis of hepatocellular carcinoma (HCC).

Purpose: To evaluate the Wnt/β-catenin pathway and MVI states using preoperative MR elastography (MRE).

Study Type: Prospective.