Transcriptomic and Lipidomic Analysis Reveals the Regulatory Network of Lipid Metabolism in .

Hemp possesses significant healthcare value due to its rich composition of unsaturated fatty acids and a distinctive golden ratio of linoleic acid to α-linolenic acid. As a promising special-oil crop, it holds substantial potential for development and utilization. However, the regulatory mechanisms underlying its lipid metabolic pathways remain poorly understood.

Synergistic Photothermal Ice Inhibition Strategy for Scalable Cryopreservation of Macroscopic Spheroids and Immune Cells in Advanced Immunotherapy.

Scalable and effective cryopreservation of human immune cells and biological samples is challenging for advanced cellular therapeutics. However, current cryoprotectants are limited due to trade-offs between robust ice inhibition and uniform thermal management to mitigate Ostwald ripening. Herein, we develop a nonpermeable nanocryoprotectant (PVA@PPy) by combining antifreezing poly(vinyl alcohol) (PVA) with photothermal polypyrrole (PPy).

Allostatic Load and Physical Frailty Increase the Risk of Depression Recurrence in Middle-Aged and Older Adults: A Prospective Cohort Study.

Aims: Individuals with prior depressive episodes have a more fragile homeostasis, but the exploration of depression recurrence remains limited. Our study aimed to explore the associations of allostatic load (AL) and frailty with the risk of depression recurrence.

Methods: This study included 9,936 individuals from the UK Biobank who reported depression prior to recruitment.

Exploring the epigenetic mechanisms of recurrent pregnancy loss: from genetic predisposition to environmental influences.

Recurrent pregnancy loss (RPL) is a complex and emotionally distressing condition defined by the occurrence of two or more consecutive miscarriages before 20 weeks of gestation. The exact causes of RPL remain unclear, despite extensive research into its genetic, immune, and environmental underpinnings. Recent advances in epigenetics have revealed that alterations in DNA methylation, histone modifications, and non-coding RNA expression play crucial roles in regulating pregnancy-related processes such as immune tolerance, placental development, and embryo implantation.

Overall Survival Associated with Real-World Treatment Sequences in Patients with CLL/SLL in the United States.

This study compared overall survival (OS) associated with common real-world treatment sequences in patients with chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) in the United States. : Utilizing the nationwide Flatiron Health electronic health record-derived de-identified database, adult CLL/SLL patients who initiated systemic therapy (JAN2016-NOV2023) and received at least two lines of therapy (LoTs) were analyzed. Treatment regimens were categorized based on drug class, and most frequent (n ≥ 50) sequences (first LoT followed by [→] second LoT) were compared.

Associations between dietary mineral intake and rheumatoid arthritis: evidence from UK biobank and NHANES.

Objectives: The relationship between dietary mineral intakes and the risk of rheumatoid arthritis (RA) remains limited. This study aims to investigate the associations between dietary mineral intakes with RA.

Methods: We evaluated the association between dietary mineral intakes and RA in 123 940 adults age 40-70 years.

Targeted prevention of radiation-induced oral mucositis by glutathione-modified liposome coated K12 probiotics and clinical study.

Radiation-induced oral mucositis (RIOM) is the most common oral complication faced by patients with head and neck cancer undergoing radiotherapy or chemotherapy, significantly diminishing their quality of life. While previous studies have investigated single K12 probiotics for RIOM, they often lacked stability, free radical scavenging activity, and precise oral targeting. To overcome these challenges, we developed K12@Lip@GSH, an innovative oral probiotic that encapsulates K12 within liposomes to enhance stability and scavenging efficacy, while utilizing a glutathione (GSH) transporter-mediated targeting mechanism that exploits the favorable the overexpression of GSH transporters in RIOM.

Influencing factors of clinical belonging among Chinese nurses in training.

This study evaluates the sense of belonging and its determinants among standardized training nurses in China. A survey of 133 nurses was conducted using convenience sampling, incorporating the Belongingness Scale-Clinical Placement Experience, Turnover Intention Scale, and Self-Rating Anxiety Scale. Data were analyzed with T-tests, ANOVA, Pearson correlation, and multiple linear regression.

Extremely low-frequency electromagnetic fields facilitate proliferation and functional differentiation in spinal neural stem cells.

Traumatic spinal cord injury (SCI), typically resulting from direct mechanical damage to the spine, often leads to disruption of neural signaling and axonal conduction, severely impairing nervous system function. In rodent models of SCI, neural stem cell (NSC) transplantation has demonstrated significant potential in restoring motor function and enhancing neural repair. Additionally, extremely low-frequency electromagnetic fields (ELF-EMFs) have demonstrated efficacy in promoting nerve regeneration and activating spinal circuits.

Enhanced homing and efficacy of HER2-CAR T cells via CXCR5/CCR6 co-expression for HER2-positive NSCLC.

Background: Chimeric antigen receptor T-cell (CAR T) therapy development represents a promising therapeutic strategy for HER2-positive non-small cell lung cancer (NSCLC), a subtype accounting for 1-5% of NSCLC cases. However, the clinical efficacy of CAR T cells remains limited by poor tumor infiltration. Here, we identify NSCLC-specific overexpression of the CXCL13 and CCL20 chemokines within the tumor microenvironment (TME) and develop a dual chemokine receptor strategy to overcome this barrier.

Manipulating the Aggregation of Phenoxazine-Modified Helical Boron-Dipyrromethene to Enhance Singlet Oxygen Generation for Efficient Tumor Therapy.

A photosensitizer that exhibits high singlet oxygen (O) generation efficiency under moderate aggregation holds promise for combined photodynamic therapy (PDT) and chemotherapy. Here, we develop a phenoxazine-modified helical boron-dipyrromethene () and manipulate its aggregation state to enhance the O generation efficiency. It demonstrates that under the moderately aggregated state, affords the highest O generation capability with a quantum yield of 53%.

Short-chain fatty acids regulate T cell heterogeneity to alleviate recurrent spontaneous abortion.

Background And Purpose: The aetiology of recurrent spontaneous abortion (RSA) is multifactorial, with immune factors playing a critical role. Gut microbiota and its metabolites have been found to participate in host immune regulation. This study explores the role of gut microbiota-derived short-chain fatty acids (SCFAs) in immune tolerance in RSA.

Plant Heteropolysaccharides as Potential Anti-Diabetic Agents: A Review.

Diabetes mellitus (DM), a chronic metabolic disease, poses a significant challenge to global health. Although type 1 diabetes mellitus (T1DM), type 2 diabetes mellitus (T2DM), gestational diabetes mellitus (GDM), and other types of diabetes mellitus differ in pathological mechanisms, they converge in that hyperglycemia is a universal clinical hallmark. Currently, the antidiabetic medications employed in clinical practice for blood glucose management require long-term administration and are associated with various side effects that can adversely impact human health.

The role of macrophages in renal fibrosis and therapeutic prospects.

Monocytes/macrophages are the key regulators of tissue repair, regeneration, and fibrosis. Monocyte-derived macrophages, which are characterized by high heterogeneity and plasticity, are recruited, activated, and polarized throughout the process of renal fibrosis in response to the local microenvironment. Increasing evidence suggests that phenotypic changes in macrophages are essential for chronic kidney disease (CKD) development and progression.

Mono or Di? A Potential-Controlled Electrochemical Halogenation of Anilines.

In organic reactions, controlling the degree of substitution, such as monosubstitution or disubstitution, typically necessitates the use of varied reagents, reaction conditions, and temperatures, often leading to increased separation costs. Simplifying this complexity while developing more direct and efficient methods for controlling reaction outcomes remains a long-standing challenge. In this work, we present a novel and efficient electrochemical strategy that leverages the precise control of electrode potential to selectively halogenate aniline derivatives, offering a breakthrough in the reaction selectivity.

Fluorine- and Trifluoromethyl-Substituted Biphenyl Derivatives: Promising Antimicrobial Agents Targeting Escherichia coli FabH.

This study investigates the synthesis, antibacterial activity, structure-activity relationships (SARs), and molecular docking of F- and CF-substituted biphenyl derivatives (5A-5K) as potential antimicrobial agents. Ten novel biphenyl derivatives were synthesized, incorporating trifluoromethyl (CF) and fluorine (F) substitutions, to evaluate their efficacy against various bacterial strains, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus pneumoniae. Antimicrobial assays demonstrated that the F substitution significantly enhanced antibacterial activity, with the bis-F derivative (5D) showing improved potency compared to the other compounds.

Utility of the joint index in newborn congenital heart disease screening.

To examine the value of"joint index"in the early and rapid screening of congenital heart disease (CHD) in newborns. The study selected live-born neonates who were delivered in the Department of Obstetrics and Gynecology at Sichuan Provincial People's Hospital from January 1, 2023 to December 31, 2023. Cardiac auscultation and pulse oximetry were performed at 6 h to 72 h after birth.

LncRNA SNHG5 promotes the invasion and proliferation of oropharyngeal squamous cell carcinoma by regulating the miR-21/PTEN signaling pathway.

Objective: To delineate the functional role of long non-coding RNA (LncRNA) SNHG5 in oropharyngeal squamous cell carcinoma (OPSCC).

Methods: The expression of LncRNA SNHG5 in paired tumor/adjacent tissues and cell lines (HN6 vs. HIOEC) were detected, and the relationship between LncRNA SNHG5 expression in cancer tissues and the clinicopathological characteristics and prognosis of patients was analyzed.

Integrated bioinformatics analysis identifies CHAD association with osteoporosis and in vitro chondrogenic effects of Wogonin.

Osteoporosis (OP), characterized by reduced bone density and increased fracture risk. Current therapies have limitations due to side effects and variable efficacy, necessitating exploration of novel therapeutic targets and alternatives like Traditional Chinese Medicine (TCM). The molecular mechanisms driving OP remain incompletely understood.

Comparative physicochemical properties and anti-inflammatory activity of natural and artificial musk for quality evaluation.

In this study, we conducted a comprehensive comparative analysis of the physicochemical properties of natural musk (NM) and artificial musk (AM), focusing on their ultrastructure, volatile constituents, alcohol-soluble compounds, amino acid composition, inorganic element content and anti-inflammatory activity. Advanced analytical techniques such as scanning electron microscopy (SEM), gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), amino acid auto analyzer (AAA), and inductively coupled plasma-mass spectrometry (ICP-MS) were utilized to evaluate the differences in their physicochemical properties. The results of the study showed that SEM could distinguish NM and AM based on their surface morphology.

COVID-19 and stroke MR study: data errors, timing gaps, statistical flaws.

This correspondence critiques the Mendelian randomization study by Zhang et al. on the causal link between COVID-19 and ischemic stroke, highlighting three methodological concerns. First, the study incorrectly attributes the eQTL data to the GTEx database, whereas the actual source is the eQTLGen Consortium, necessitating correction for accurate data provenance.

Prevalence and risk factors of portal vein thrombosis following hepatectomy: a systematic review and meta‑analysis.

Introduction: The prevalence and risk factors of portal vein thrombosis (PVT) are largely unclear, with an increasing number of studies reporting inconsistent results.

Aim: The current study aimed to evaluate the prevalence and risk factors of PVT following hepatectomy through a systematic review and meta‑analysis.

Materials And Methods: A comprehensive literature search was conducted across multiple databases (PubMed, Embase, and the Cochrane Library) to identify relevant studies.

The Oncologist, 2025, Vol, XX, Issue XX Predictive role of ARID1A and B2M mutations and the antigen presentation pathway in the efficacy of definitive chemoradiotherapy for cervical cancer.

Background And Purpose: Definitive chemoradiotherapy (dCRT) is the standard treatment for locally advanced cervical cancer (LACC), yet patients experience considerable variability in disease-free survival (DFS). This study aimed to identify molecular biomarkers associated with response to dCRT in cervical cancer.Materials and methods: We retrospectively analyzed targeted next-generation sequencing data from tumor biopsy samples of 31 patients diagnosed with FIGO stage IIB-IVA LACC.

Fluoro-Substitution of BODIPY Derivatives to Enhance Photothermal Conversion Efficiency for Near Infrared II Fluorescence Imaging and Immuno-photothermal Therapy.

Immuno-photothermal therapy (IPTT) has emerged as a promising cancer treatment strategy. Developing photothermal agents (PTAs) with high performance is crucial in IPTT. Herein, a facile and efficient strategy is developed to enhance the photothermal conversion efficiency (PCE) of PTAs via multifluorination.

Integrating gene mutation spectra from tumors and the general population with gene expression topological networks to identify novel cancer driver genes.

Discovering cancer driver genes is critical for improving survival rates. Current methods often overlook the varying functional impacts of mutations. It is necessary to develop a method integrating mutation pathogenicity and gene expression data, enhancing the identification of novel cancer drivers.