Molecular characterization of a novel narnavirus from the plant-pathogenic fungus Colletotrichum gloeosporioides.

Here, we characterized a novel mycovirus, Colletotrichum gloeosporioides narnavirus 1 (CgNV1), isolated from Colletotrichum gloeosporioides strain T-19, a fungal pathogen responsible for anthracnose in various crops. The CgNV1 genome consists of a 3,628-nucleotide-long positive-sense single-stranded RNA (+ ssRNA) with a GC content of 51.93% and has a single open reading frame (ORF) encoding an RNA-dependent RNA polymerase (RdRp) of 1,155 amino acids (132.

Unraveling morphological brain network disparities Parkinsonian tremor from essential tremor: an artificial intelligence approach for clinical differentiation.

Tremor-dominant Parkinson's disease (TD) and Essential Tremor (ET) are the two most common types of tremors, posing huge challenges in diagnosis. This study was to investigate the pathogenesis of tremors using brain morphology and employ artificial intelligence techniques for distinguishing them. The cortical thickness differences in TD were primarily centered on the right precuneus, while in ET were mainly observed in the right medial orbitofrontal cortex.

Distribution Characteristics of Vertebral CT Hounsfield Unit Values in Patients With Degenerative Lumbar Scoliosis.

Study DesignRetrospective study.ObjectiveThis study aims to identify how CT HU values vary in degenerative lumbar scoliosis (DLS) patients with different scoliotic apexes and in those with different degree of scoliosis.MethodsWe included 222 DLS patients and 140 lumbar spinal stenosis (LSS) patients, dividing the DLS patients into 2 groups based on scoliotic apex location.

The application of mixed reality navigation system in robot-assisted radical prostatectomy for high-risk prostate cancer: a propensity score‑matched cohort study.

Background: This study aims to evaluate the efficacy and advantages of MR navigation system in robot-assisted radical prostatectomy (RARP) for high-risk prostate cancer (PCa).

Methods: We retrospectively studied 147 patients with high-risk PCa based on D'Amico risk criteria from July 2021 to November 2023. All patients chose MR-assisted RARP (MR-RARP) or standard RARP (S-RARP) after receiving comprehensive counseling on the benefits and risks of both procedures.

The health burden of disease attributable to low calcium intake: a comprehensive analysis of trends and socioeconomic impacts from 1990 to 2021.

Background: Calcium, a vital nutrient for the human body, is indispensable for keeping our bones strong and managing cell function. A diet low in calcium (DLC) is a key player in the formation of numerous health issues. This research delved into the most recent datasets acquired via the 2021 Global Burden of Disease (GBD) report to uncover the worldwide impact of DLC.

Self-Assembled Nanobody-Albumin Platform with Dye Encapsulation for NIR-II Imaging-Guided Photodynamic Therapy.

Nanobodies have emerged as a promising alternative to full-length immunoglobulin G (IgG) in a wide range of biomedical applications. However, the low molecular weight of nanobodies causes a limited drug-loading capacity and rapid renal clearance in vivo, leading to diminished delivery efficiency. Here, we describe a tumor-targeted nanobody platform that employs albumin binding and dye encapsulation for NIR-II imaging-guided photodynamic therapy.

Structure characterization and repair of UVB-induced skin inflammation of rhamnomannan derived from Roseomonas mucosa DL-1.

The skin microbiome plays a crucial role in both (epi-) dermal immune and defense responses, with the skin commensal microbiota being closely associated with various skin diseases. While the therapeutic applications of skin microbiota are expanding, there are still gaps in the understanding of specific mechanisms and applications. In this study, a low-molecular-weight exopolysaccharide (DLEPS) derived from Roseomonas mucosa DL-1 was structurally analyzed using nuclear magnetic resonance and methylation techniques.

Distinct structural mechanisms of LGR4 modulation by Norrin and RSPOs in Wnt/β-catenin signaling.

The Wnt/β-catenin pathway requires precise regulation for proper development and tissue homeostasis, yet the structural mechanisms enabling its fine-tuned control remain incompletely understood. Here, we reveal how LGR4 achieves differential signaling outcomes through distinct recognition of two key modulators: Norrin and R-spondins (RSPOs). Using cryo-electron microscopy, we determined the structure of full-length LGR4 bound to Norrin in a 2:2 stoichiometry, revealing a molecular bridging mechanism where Norrin dimer connect two LGR4 protomers in a spatial arrangement fundamentally distinct from the LGR4-RSPO2-ZNRF3 complex.

Analysis of the global trends and causes of self-harm due to high temperature: a global level ecological study.

Background: High temperatures are known to be associated with an increased risk of self-harm, but the influence of demographic changes and country-level indicators on the burden of heat-related self-harm remains unclear. This study examined the key factors driving changes in self-harm mortality linked to high temperatures and explored their impact at the country level.

Methods: This is an ecological study that analyzes data from the 2021 Global Burden of Disease (GBD) study, the World Bank, and the Climate Research Unit (CRU) were analyzed.

Photocatalytic Synthesis of Hydrogen Peroxide by Rhenium Modified Metal-Organic Frameworks Incorporating Bianthracene Ligands.

Photocatalytic synthesis of hydrogen peroxide (HO) from oxygen (O) is a challenging process. Metal-organic framework (MOF) materials are emerging photocatalysts with potential tunable light absorption properties. Herein, we report a rhenium (Re) modified Zr-based MOF, Re10-MFM-67, in which active Re sites are incorporated into MFM-67 by partial replacement of 9,9'-bianthracene-10,10'-dicarboxylic acid (HL) with a [(HL)Re(CO)Cl] (HL = 2,2'-bipyridine-5,5'-dicarboxylic acid) moiety.

Integrating jigsaw teaching into self-regulated learning instruction: an instructional design to improve nursing students' self-regulated learning.

Objective: This study aimed to investigate the impact of integrating Jigsaw teaching into Self-regulated learning instruction on nursing students' self-regulated learning ability and engagement.

Methods: This quasi-experimental study was conducted with 94 nursing students from Hubei University of Traditional Chinese Medicine (Hubei, China) in two classes during the fourth education semester. One classroom was assigned to the control group (43) and the other to the intervention group (50).

MicroRNA miR-193b-3p Regulates Esophageal Cancer Progression Through Targeting RSF1.

Esophageal cancer (ESCA) is the sixth leading cause of cancer-related mortality worldwide. Despite the significant impact, the molecular mechanisms underlying its initiation and progression remain poorly understood. In this study, we identified mircoRNA miR-193b-3p as a critical regulator of ESCA progression and the Remodeling and Spacing Factor 1 (RSF1) as an essential target of miR-193b-3p.

Integrating Acupuncture and Herbal Medicine into Assisted Reproductive Technology: A Systematic Review and Meta-Analysis of East Asian Traditional Medicine.

Background: Assisted reproductive technologies (ARTs) are essential in treating infertility but often face limited success due to low implantation and live birth rates. East Asian traditional medicine (EATM), including acupuncture and herbal medicine (HM), may enhance physiological responses during ART cycles. This study evaluated the effectiveness and safety of EATM in improving clinical pregnancy and live birth outcomes in women undergoing ART.

Exploring factors influencing health impact assessment policy identification in China: a comparative study of health and nonhealth sectors.

Background: Existing research indicates that both subject trust and procedural justice exert an influence on government staff's level of identification with health impact assessment (HIA) policies, revealing notable differences in attitudes, comprehension levels and preferences for implementing HIA policies among staff from different sectors. There is an urgent requirement to develop an integrated research model that systematically investigates the extent of HIA policy identification and its underlying drivers, commencing with the perspectives of staff from health and nonhealth sectors alike.

Methods: A questionnaire survey using a multistage stratified random sampling method was conducted among health sector staff (n = 247) and nonhealth sector staff (n = 408) in Zhejiang province in China.

GSTM1 suppresses cardiac fibrosis post-myocardial infarction through inhibiting lipid peroxidation and ferroptosis.

Background: Cardiac fibrosis following myocardial infarction (MI) drives adverse ventricular remodeling and heart failure, with cardiac fibroblasts (CFs) playing a central role. GSTM1 is an important member of the glutathione S-transferase (GSTs) family, which plays an important role in maintaining cell homeostasis and detoxification. This study investigated the role and mechanism of GSTM1 in post-MI fibrosis.

Potential Influence of Sagittal Parameters in the Pathogenesis of Patients With the Thoracic Ossification of the Ligamentum Flavum.

Purpose: There are many causes of thoracic ossification of the ligamentum flavum (TOLF), of which mechanical stress factors have gained increasing attention. This study aimed to explore the potential pathogenesis of TOLF by comparing spinal sagittal alignment parameters between patients with TOLF and an asymptomatic population.

Methods: We retrospectively included patients who underwent surgical treatment for thoracic myelopathy caused by OLF at Peking University Third Hospital from 2012 to 2022.

New insights on metabolic reprogramming in macrophage plasticity.

Macrophages are the first line of defense in the innate immune system. Macrophages have two subtypes: classically activated macrophages (M1) and alternatively activated macrophages (M2), with different phenotypes and functions. They play a critical role in defending against pathogens and maintaining internal homeostasis.

Curcumin: A Magical Small Molecule with a Large Role in Active-Intelligent Degradable Food Packaging.

Curcumin exhibits antioxidant, antibacterial, antitumor, and anti-inflammatory biological properties. Its dual functionality as both a food additive and a pH-sensitive colorant has led to extensive applications in meat products and other food systems, thereby garnering significant research interest. In recent years, curcumin-loaded active-intelligent food packaging films have emerged as a promising innovation due to their multifunctional capabilities: not only do they prevent microbial contamination and extend food shelf life, but they also enable real-time freshness monitoring through visual colorimetric responses.

Changes of brain structure and structural covariance networks in Parkinson's disease with different sides of onset.

Background: Parkinson's disease (PD) typically presents with unilateral symptoms in early stages, starting on one side and progressing, with the onset side showing more severe motor symptoms even after bilateralization. This asymmetry may reflect complex interactions among multiple brain regions and their network connections. In this study, we aimed to use surface-based morphometry (SBM) and structural covariance networks (SCNs) to investigate the differences in brain structure and network characteristics between patients with left-onset PD (LPD) and right-onset PD (RPD).

Screening and functional studies of Norrin-related nanobodies.

Norrin is a crucial regulator of the Wnt/β-catenin signaling pathway, playing a key role in retinal vascular development, blood-brain barrier maintenance, and neuroprotection. In this study, a Norrin fusion protein (Norrin-Fc) was successfully expressed and purified using an insect cell expression system. Camels were immunized with purified Norrin-Fc to generate nanobodies, which consist solely of the heavy chain variable domains of heavy chain antibodies (VHH) with a molecular weight of approximately 15 kDa.

Radiomics-based assessment of HER2 status and prognosis in gastric cancer: a retrospective dual-center CT study.

Purpose: This research investigated the potential of CT-based radiomics analysis for predicting human epidermal growth factor receptor 2 (HER2) status and assessing the prognosis of patients with gastric cancer (GC).

Methods: 431 patients with GC from two medical centers were included in this retrospective study, with patients allocated to a training cohort (n = 221), a testing cohort (n = 94), and an external validation cohort (n = 116). Radiomics features and clinical variables associated with HER2 status were identified, and the radiomics score was subsequently derived.

Differences in symptom clusters based on multidimensional symptom experience and symptom burden in stroke patients.

In the study of stroke symptoms, a significant unresolved issue remains: What are the similarities and differences in the use of three symptom dimensions-occurrence, severity, and distress-and symptom burden to identify symptom clusters, and which level is recommended for constructing symptom clusters? This study aimed to identify the number and types of symptom clusters in stroke patients on the basis of these dimensions and to determine the most suitable dimension for extracting symptom clusters. Data were collected from 656 stroke patients via a convenience sampling method at a tertiary-level hospital in Wuhan, China, between August 2023 and March 2024. Exploratory factor analysis was conducted to extract symptom clusters on the basis of the three dimensions of the symptom experience scale and symptom burden.

Design of NanoBiT-Nanobody-based FGL1 biosensors for early assisted diagnosis of esophageal cancer.

Esophageal cancer (EC) is one of the most common causes of cancer-related mortality due in part to challenges in early diagnosis. Biomarker identification is crucial for improved early screening and treatment strategies for patients. Firstly, we employed serum proteomics techniques to screen for potential biomarkers in 15 early-stage EC patients and 5 healthy individuals.