Dendrobin A inhibits gastric cancer: Mechanistic insights supported by integrated evidence.

Background: Traditional Chinese medicine (TCM) has shown anti-tumor potential, but its molecular mechanisms remain poorly understood. This integrated bioinformatics, network pharmacology, and experimental study investigated the anti-cancer effects and mechanisms of Dendrobin A, a pharmacologically active bibenzyl compound from Dendrobium nobile, in gastric cancer (GC).

Methods: Differentially expressed genes (DEGs) were identified through analysis of the TCGA-STAD dataset.

Molecular investigation of the progenitors, origin, and domestication patterns of diploid Chinese old garden roses.

Background And Aims: Chinese old garden roses are major contributors in the genetic development of modern roses. The RoKSN gene is associated with continuous flowering in roses and is proposed to have originated from Chinese wild roses. However, the wild roses that are implicated in the breeding of Chinese old garden roses and the origin of the RoKSN locus remain unidentified.

Replication competition drives the selective mtDNA inheritance in Drosophila ovary.

Purifying selection that limits the transmission of harmful mitochondrial DNA (mtDNA) mutations has been observed in both human and animal models. Yet, the precise mechanism underlying this process remains undefined. Here, we present a highly specific and efficient in situ imaging method capable of visualizing mtDNA variants that differ by only a few nucleotides at single-molecule resolution in Drosophila ovaries.

Decision-making in valve reintervention: Redo-Transcatheter aortic valve replacement vs. explant surgery.

Objective: Data remain limited on factors influencing the selection of redo-transcatheter aortic valve replacement (TAVR) versus surgical explant in patients with failing transcatheter heart valves. This study aimed to identify clinical and procedural factors guiding treatment decisions.

Methods: This single-center, retrospective study included all patients who underwent aortic valve reintervention following prior TAVR at a U.

Intelligent bacterial platform induces programmed death-ligand 1 lysosomal degradation to enhance antitumor immunity.

Engineered bacteria have emerged as a promising therapeutic strategy for tumor therapy by exploiting the unique features of the tumor microenvironment (TME). In this study, we developed a low-endotoxin Escherichia coli expression system to produce a fusion protein comprising the CXCL12 with the nanobody KN035 Hypoxia and immunosuppression drive the selective colonization of engineered bacteria in tumor tissues, minimizing host toxicity. Unlike conventional PD-L1 antibodies that merely block immune checkpoints, our CXCL12-KN035 fusion protein simultaneously targets the CXCR7 receptor and PD-L1 on tumor cells, inducing receptor internalization and subsequent lysosomal degradation of PD-L1.

Exploring the impact of age on microvascular function: Insights from the coronary microvascular disease registry.

Background: Coronary microvascular dysfunction (CMD) contributes to angina without obstructive coronary artery disease (ANOCA). Although aging is known to impact cardiovascular health, its effect on coronary microvascular function remains unclear. This study examined the relationship between age and microvascular function in patients with ANOCA.

Three-Dimensional Magnetic Field Measurement of Transcranial Magnetic Stimulation Using a Printed Circuit Board-Based Miniature Orthogonal Coil Array.

Objective: Transcranial magnetic stimulation (TMS) is a promising neuromodulation therapy for treating diseases such as depression and Alzheimer's disease. However, its efficacy depends on precise magnetic field targeting. Current measurement methods face a trade-off between accuracy and complexity.

Digital risk prediction model for transoral surgery in patients with atlantoaxial dislocation and sandwich fusion.

Purpose: In patients presenting with the sandwich fusion, characterized by C1 occipitalization and C2-C3 non-segmentation, leading to stress concentration at the atlantoaxial joint, there is an increased likelihood of atlantoaxial dislocation (AAD). The decision to proceed with transoral surgery is contingent upon the outcomes of intraoperative traction assessment. The complexity of intraoperative decision-making introduces a degree of uncertainty in preoperative planning, complicating both the surgical preparation and doctor-patient communication.

Training of physical neural networks.

Physical neural networks (PNNs) are a class of neural-like networks that make use of analogue physical systems to perform computations. Although at present confined to small-scale laboratory demonstrations, PNNs could one day transform how artificial intelligence (AI) calculations are performed. Could we train AI models many orders of magnitude larger than present ones? Could we perform model inference locally and privately on edge devices? Research over the past few years has shown that the answer to these questions is probably "yes, with enough research".

Development of a validated and sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method to simultaneous determination of crizotinib, alectinib and lorlatinib in human plasma.

Crizotinib, alectinib, and lorlatinib are tyrosine kinase inhibitors used sequentially in non-small cell lung cancer (NSCLC) therapy. Therapeutic drug monitoring (TDM) is valuable during sequential treatment, enabling simultaneous quantification. Consequently, an analytical method capable of multiplexing these three compounds into a single assay is highly applicable for routine TDM.

China Expert consensus on the application of metagenomic next-generation sequencing for the etiological diagnosis of infections in hematological disorders (2024).

Infections are frequent complications in patients with hematological disorders, and pathogen diagnosis remains challenging. Metagenomic next-generation sequencing (mNGS) is an unbiased high-throughput technology that has been widely applied in the diagnosis of infectious diseases. However, to date, there are no established international guidelines or expert consensuses regarding the use of mNGS to diagnose infections in patients with hematologic disorders.

Assessment of heavy metal pollution and health risks in urban park soil of Chengdu, China: implications for ecosystem management and public health.

Despite Chengdu's status as China's first 'park city', systematic assessment of heavy metal risks in its urban green spaces remains limited. This research investigates the concentrations and spatial distribution of heavy metals, including Mercury (Hg), Chromium (Cr), Arsenic (As), Cadmium (Cd), Lead (Pb), Copper (Cu), Nickel (Ni) and Zinc (Zn) in the surface soil of urban parks in Chengdu, China. The results indicate significant differences in metal concentrations, with higher median values for Hg, Cd, Ni, and Zn than that in suburban areas (p < 0.

Polymerization of L-Arginine into Nanomicelles for Immunometabolic Engineering of Adoptive Macrophages in Solid Tumor Therapy.

The therapeutic efficacy of adoptive cell therapy is highly dependent on the status and function of the infused cells. However, insufficient nutrient availability within the immunosuppressive tumor microenvironment (TME) often impedes these cells from fully exerting their cytotoxic potential against solid tumors. Here, we present a strategy of integrating adoptively transferred macrophages with intracellular nutrient depots composed of L-arginine-based nanomicelles to provide a sustainable supply of essential metabolite and optimize the cellular activity in the nutrient-deprived TME.

Protein-Templated Fe and Ni Subnanoclusters for Advanced Energy Storage and Electrocatalysis.

Downsizing metal nanoparticles into nanoclusters and single atoms represents a transformative approach to maximizing atom utilization efficiency for energy applications. Herein, a bovine serum albumin-templated synthetic strategy is developed to fabricate iron and nickel nanoclusters, which are subsequently hydrothermally composited with graphene oxide. Through KOH-catalyzed pyrolysis, the downsized metal nanoclusters and single atoms are embedded in a hierarchically porous protein/graphene-derived carbonaceous aerogel framework.

The Non-Traditional Cardiovascular Culprits in Chronic Kidney Disease: Mineral Imbalance and Uremic Toxin Accumulation.

Chronic kidney disease (CKD) is associated with a significantly elevated mortality rate, primarily due to cardiovascular disease (CVD), highlighting a complex bidirectional relationship between the two conditions. Life-threatening cardiovascular events occur despite control of the traditional risk factors, emphasizing the underlying role of non-traditional risk factors. CKD, causing mineral imbalance and the accumulation of uremic toxins due to a compromised ability to excrete waste products, imposes extra pressure on the cardiovascular system.

Precision intervention of virtual reality training for balance and gait in Parkinson's disease: a dose-response meta-analysis.

Background: This study systematically evaluated the effects of virtual reality training (VRT) on balance ability and functional gait in Parkinson's disease (PD) patients and used dose-response analysis to quantify optimal intervention parameters, providing evidence-based guidance for clinical rehabilitation.

Methodology: This systematic review and meta-analysis followed PRISMA guidelines and was registered with PROSPERO (CRD420251008459). Six databases, including PubMed, Embase, and Cochrane Library, were searched for RCTs published before March 15th, 2025.

Multidimensional insights into exosomes in hepatocellular carcinoma: from genesis to clinical application.

Hepatocellular carcinoma (HCC) is a highly aggressive malignancy, whose progression is intimately linked to the complex dynamics of the tumor microenvironment (TME). Exosomes, once considered mere cellular waste, have emerged as pivotal mediators of intercellular communication within the TME, actively participating in the multistep development of HCC. These nanoscale vesicles play crucial roles in the initiation of precancerous lesions and, by transporting drug resistance-related molecules such as proteins and non-coding RNAs, facilitate the acquisition of resistance to chemotherapy and targeted therapies by tumor cells.

Failure Mode and Effects Analysis (FMEA) integrating quality indicators for risk assessment of the total testing process in human papillomavirus genotyping testing: a proactive risk analysis model for molecular diagnostics.

Objectives: To develop a proactive risk assessment model for human papillomavirus (HPV) genotyping testing by integrating Failure Mode and Effects Analysis (FMEA) with quality indicators (QIs), ensuring compliance with ISO 15189:2022 and improving diagnostic accuracy.

Methods: A multidisciplinary team designed and performed detailed FMEA across pre-analytical, analytical, and post-analytical phases of HPV genotyping testing. To improve objectivity, we integrated Sigma metrics into the FMEA framework through a molecular diagnostics-specific model of QIs (MQI).

Recent advances in heterostructure-based organic field-effect transistor memory.

With ongoing advancements, heterojunction structures have been widely adopted in the memory domain, delivering enhanced performance and superior properties beyond those offered by single-layer thin films. In particular, among various organic field-effect transistor memory (OFETM) technologies, heterojunction-based OFETMs have emerged as one of the most promising data storage technologies, with applications ranging from sensory and storage devices to neuromorphic computing. Heterostructure-based OFETMs can achieve a memory window of up to 90 V, a high current on/off ratio of 10, and retention characteristics exceeding 10 years.

Non-Viral Gene Delivery Systems: Current Advances and Therapeutic Applications.

Gene therapy holds immense potential for treating genetic disorders, malignancies, and infectious diseases through the targeted introduction, silencing, or precise editing of therapeutic genes. Although viral vectors exhibit exceptionally high gene transfection efficiency, their clinical application faces significant challenges, including robust immunogenicity, the insertional mutagenesis risks, complex and costly manufacturing processes hindering large-scale manufacturing, limited gene cargo capacity, and poor packaging efficiency for large genes. In contrast, nonviral vectors-such as lipid nanoparticles (LNPs), cationic polymers, and inorganic nanoparticles, offer numerous advantages, including superior safety profiles, the scalability for manufacturing, structural and functional reconfigurability in accommodating various sizes cargo.

Clinical Characteristics and Prognosis of Patients with Severe Pneumonia with Neurological Dysfunction: A Regional Multicenter Retrospective Study in Mainland China.

Purpose: Pneumonia is common in ICU patients with neurological dysfunction, but differences in pulmonary pathogen distribution in this population remain unclear. This study aimed to compare pathogen profiles, clinical features, and outcomes between ICU patients with and without neurological dysfunction.

Methods: This regional multicenter retrospective study included adult patients with severe pneumonia admitted to intensive care units (ICUs) in 11 hospitals across Zhejiang and Henan Provinces in mainland China between December 2018 and November 2023.

De novo design mini-binder proteins targeting the capsid-forming domain of type 2 PRRSV nucleocapsid protein to inhibit PRRSV replication in vitro.

Porcine reproductive and respiratory syndrome virus (PRRSV) has caused huge economic losses to swine industry but there is no effective antiviral drug. Nucleocapsid (N) protein is highly conserved in type 2 PRRSV and is considered as an important target for antiviral development. Mini-binders are novel protein drugs de novo designed for a specific protein target using a computational approach, which has great application prospects.

Spatially-confined bubble plasma oxidation induced by microchannel discharge to boost energy efficiency in water treatment.

Water pollutants, in particular emerging contaminants, pose severe risks to aquatic ecosystems and public health due to their high persistence and bioaccumulation potential. While advanced oxidation processes (AOPs) offer sustainable solutions for pollutant degradation, plasma-based AOPs often suffer from low energy efficiency and slow mass transfer of active species. Here we report a spatially-confined plasma AOP, generated in situ by electrical discharge within microchannel during bubble formation.

[Expression of Concern] MicroRNA‑195 targets VEGFR2 and has a tumor suppressive role in ACHN cells via PI3K/Akt and Raf/MEK/ERK signaling pathways.

Following the publication of the above paper, it was drawn to the Editor's attention by a concerned reader that, regarding the Transwell migration and invasion assay data shown in Fig. 3A and B, two pairs of panels appeared to contain overlapping sections of data (out of a total of ten panels), such that data which were intended to show the results from differently performed experiments appeared to have been derived from the same original sources. Upon analyzing the data independently in the Editorial Office, a third pair of data panels in the same figure were found to be similarly affected.