Degradation of Structure Variants of Boric Acid Channels through ERAD Pathway in Arabidopsis.

The nodulin 26-like intrinsic protein NIP5;1 is a boric acid channel localized in the plasma membrane (PM) for efficient uptake of B in roots of Arabidopsis thaliana under low B conditions. NIP6;1 is the closest paralog of NIP5;1 and is responsible for B distribution to young tissues in shoots. In the present study, we analyzed the contribution of the N-terminal cytosolic region of the boric acid channels in their localization and identified critical leucine residues at the boundary of the N-terminal cytosolic region and the 1st transmembrane helix (L76 of NIP5;1 and L78 of NIP6;1).

Mechanism for the decrease of Cd uptake and transport within wheat plants in the presence of dissolved Fe(II).

Dissolved Fe(II) influences Cd uptake and transport within wheat plants, but the specific interaction process remains elusive. This study used synchrotron radiation technology, Fourier transform infrared spectroscopy, high-performance liquid chromatography, ion chromatography, and RT-PCR to analyze the interaction processes of Fe(II) and Cd(II) ions in the roots of wheat grown in hydroponic systems containing Cd(II). The results indicated that dissolved Fe(II) decreases Cd uptake due to rhizosphere passivation, cell wall binding or blocking, and competitive absorption and regulation.

Arsenite transporter OsNIP3;5 modulates phosphate starvation responses via regulating arsenite translocation in rice.

The plants inevitably absorb toxic arsenate [As(V)] through phosphate (Pi) transporters (PTs) in As-contaminated soils due to the structural similarity between phosphate and arsenate As(V). Plants suppress PT's expression to reduce As(V) uptake when As accumulates in the cytosol. However, how plants maintain efficient Pi uptake under As(V) stress conditions remains unknown.

Hydrogen Peroxide Positively Regulates Phosphate Starvation Responses in Rice.

Phosphorus is an essential macronutrient for plant growth and development. Under phosphate (Pi) starvation conditions, plants activate a series of adaptive responses, among which reactive oxygen species (ROS) accumulation in root tissues represents a notable yet poorly characterized phenomenon. This study investigated the regulatory role of hydrogen peroxide (HO) in rice adaptation to Pi deficiency through pharmacological intervention using potassium iodide (KI), a specific HO scavenger.

Imbalance between boron and phosphorus supply influences boron deficiency symptoms in Brassica napus L.

Background: The occurrence of boron (B) deficiency in Brassicaceae crops has increased in recent years. Inappropriate application of B with other nutrients often exacerbates symptoms of B deficiency. The aim of this study was to explore the interactive effects of B and phosphorus (P) on the B deficiency symptoms of rapeseed (Brassica napus L.

Transcriptional Analysis Reveals the Differences in Response of Floral Buds to Boron Deficiency Between Two Contrasting Varieties.

Boron (B) is an essential micronutrient for the development of crops, and its reproductive stage is particularly sensitive to B deficiency. L., as an important oil-crop species, is extremely vulnerable to B deficiency.

OsMYB67 Knockout Promotes Rice Heading and Yield by Facilitating Copper Distribution in Panicles.

Copper (Cu) is an essential micronutrient required for rice flowering and seed setting. Here, we identified that Cu-induced R2R3-MYB transcription factor, OsMYB67, acts as a negative regulator that controls rice heading and yield production by affecting Cu distribution in panicles. OsMYB67 was constitutively expressed, with the highest expression in the roots.

Integrated analysis of physiological and metabolic data uncovers essential dynamic mechanisms involved in the maturation of cigar tobacco leaves.

The quality of cigar tobacco leaves is profoundly affected by the timing of their harvest, with both early and late collections resulting in inferior characteristics. While the relationship between maturity and physiological metabolic processes is acknowledged, a comprehensive understanding of the physiological behavior of cigar leaves harvested at different stages remains elusive. This research investigated the physiological and metabolomic profiles of the cigar tobacco variety CX-014, grown in Danjiangkou City, Hubei Province, with leaves sampled at 35 (T1), 42 (T2), 49 (T3), and 56 (T4) days post-inflorescence removal.

The BnamiR827-BnaA09.NLA1-BnaPHT1 module regulates phosphate homeostasis, pollen viability, and seed yield in Brassica napus.

Phosphorus (P) is an essential macronutrient for the growth and yield of crops. However, there is limited understanding of the regulatory mechanisms of phosphate (Pi) homeostasis, and its impact on growth, development, and yield-related traits in Brassica napus. Here, we identified four NITROGEN LIMITATION ADAPTATION1 (BnaNLA1) genes in B.

Transcriptome and phytohormone profiling of stamen and pistil in Brassica napus under boron deficiency.

Plant reproduction is a fundamental requirement for plants to sustain genetic inheritance. In the perspective of plant nutrition, such process is strongly influenced by boron deficiency (-B) and as documented about a century ago. To date, little is known about the mechanism of boron deficiency-induced fertility reduction.

Transcription factor AtNAC002 positively regulates Cu toxicity tolerance in Arabidopsis thaliana.

Copper (Cu) is an essential micronutrient for plant growth and development, but environmental Cu pollution has become increasingly severe, adversely affecting both ecosystems and crop productivity. In this study, we identified the AtNAC002 gene as a positive regulator of Cu toxicity in Arabidopsis thaliana. We found that AtNAC002 expression was induced by Cu excess, and the atnac002 mutant was Cu-sensitive, accumulating more Cu than the wild-type.

Long-term adaptation of water hyacinth to low cadmium involves antioxidant enzyme and metallothionein transcriptional regulation.

Cadmium (Cd) is one of the main heavy metal pollutants in environment. Water hyacinth (Eichhornia crassipes) is an effective phytoremediation plant for mitigating Cd stress, though the concentration threshold for its long-term survival remains unclear. Our results indicate that 4 mg L Cd may be the maximum threshold for long-term cultivation of water hyacinth, as it significantly inhibits root growth and photosynthesis.

Circadian Rhythm and Nitrogen Metabolism Participate in the Response of Boron Deficiency in the Root of .

Boron (B) deficiency has been shown to inhibit root cell growth and division. However, the precise mechanism underlying B deficiency-mediated root tip growth inhibition remains unclear. In this study, we investigated the role of , a gene encoding a boric acid channel, in ().

BnaC4.BOR2 mediates boron uptake and translocation in Brassica napus under boron deficiency.

Boron (B) is an essential microelement in plant growth and development. However, the molecular mechanisms underlying B uptake and translocation in Brassica napus are poorly understood. Herein, we identified a low-B (LB)-inducible gene, namely BnaC4.

Mechanism for Fe(III) to decrease cadmium uptake of wheat plant: Rhizosphere passivation, competitive absorption and physiological regulation.

The presence of dissolved Fe(III) and Fe(III)-containing minerals has been found to alleviate cadmium (Cd) accumulation in wheat plants grown in Cd-contaminated soils, but the specific mechanism remains elusive. In this work, hydroponic experiments were conducted to dissect the mechanism for dissolved Fe(III) (0-2000 μmol L) to decrease Cd uptake of wheat plants and study the influence of Fe(III) concentration and Cd(II) pollution level (0-20 μmol L) on the Cd uptake process. The results indicated that dissolved Fe(III) significantly decreased Cd uptake through rhizosphere passivation, competitive absorption, and physiological regulation.

Golgi-localized APYRASE 1 is critical for Arabidopsis growth by affecting cell wall integrity under boron deficiency.

Many nucleoside triphosphate-diphosphohydrolases (NTPDases/APYRASEs, APYs) play a key role in modulating extracellular nucleotide levels. However, the Golgi-localized APYs, which help control glycosylation, have rarely been studied. Here, we identified AtAPY1, a gene encoding an NTPDase in the Golgi apparatus, which is required for cell wall integrity and plant growth under boron (B) limited availability.

Proteomics reveals the significance of vacuole Pi transporter in the adaptability of to Pi deprivation.

Vacuolar Pi transporters (VPTs) have recently been identified as important regulators of cellular Pi status in and . In the oil crop , and are two homologs of , the vacuolar Pi influx transporter in . Here, we show that Pi deficiency induces the transcription of both homologs of genes in leaves.

BnaA4.BOR2 contributes the tolerance of rapeseed to boron deficiency by improving the transport of boron from root to shoot.

Boron (B) is essential for plant growth. However, the molecular mechanism of B transport in rapeseed (Brassica napus L.) is unknown well.

The Mechanical Properties and Microstructure of Tailing Recycled Aggregate Concrete.

The aim of this study was to develop sustainable concrete by recycling concrete aggregates from steel waste and construction waste (iron ore tailings (IOTs) and recycled coarse aggregates (RCAs)) to replace silica sand and natural coarse aggregates. In experimental testing, the compressive strength, peak strain, elastic modulus, energy dissipated under compression, and compressive stress-strain curve were analyzed. Microscopically, scanning electron microscopy and energy-dispersive spectrometry were employed to investigate the microstructural characteristics of the interfacial transition zone (ITZ), and the results were compared with the ITZs of natural aggregate concrete and recycled aggregate concrete (RAC).