A rapid and ultrasensitive CRISPR/Cas12a-based assay for the accurate identification of T-even type phages.

Phage contamination poses a significant threat to industrial fermentation, leading to substantial economic losses. Virulent T-even type phages (T2/T4/T6) represent particularly concerning biological hazards in fermentation systems. This paper developed a novel CRISPR/Cas12a-based system integrated with recombinase polymerase amplification (RPA), enabling ultrasensitive identification of T-even type phages.

Exercise Modulates Exocytosis: Chemical Insights from the Intracellular Vesicle Perspective.

The systemic benefits of exercise are partially attributed to exerkines release via extracellular vesicles, prompting extensive research on the effects of exercise on these vesicles. However, whether and how exercise modulates intracellular vesicle chemistry (e.g.

Study of the Solid Solubility Limit and Growth of CdMnTe Multicomponent Single Crystals for Miniaturized Magneto-Optical Isolators.

CdMnTe (CMT) is a promising magneto-optical (MO) material for the visible to near-infrared region with a tunable bandgap via manganese (Mn) doping. Although CMT has been extensively studied, the solid solubility limit of Mn in CdTe (cadmium telluride) remains unclear, and the growth of high-Mn-content single crystals has not yet been reported in detail. In this work, the Mn solubility limit in CdTe was determined to be = 0.

A comprehensive evaluation of large language models for information extraction from unstructured electronic health records in residential aged care.

Despite rapid healthcare digitization, extracting information from unstructured electronic health records (EHRs), such as nursing notes, remains challenging due to inconsistencies and ambiguities in clinical documentation. Generative large language models (LLMs) have emerged as promising tools for automating information extraction (IE); however, their application in real-world clinical settings, such as residential aged care (RAC), is limited by critical gaps. Prior studies have often focused on structured EHR data and conventional evaluation metrics such as accuracy and F1 score, overlooking critical aspects like robustness, fairness, bias, and contextual relevance, particularly in unstructured clinical narratives.

Giant osmotic power density generation with an anion-selective AB-stacking covalent-organic framework bilayer.

Atomically thin two-dimensional membranes are promising for osmotic energy generation. However, the trade-off between permeability and selectivity remains a long-standing bottleneck. Herein, we demonstrate that a cationic AB-stacking covalent-organic framework (COF) bilayer achieves high ion conductivity and selectivity.

DGS-Yolov7-Tiny: a lightweight pest and disease target detection model suitable for edge computing environments.

Pest detection is vital for maintaining crop health in modern agriculture. However, traditional object detection models are often computationally intensive and complex, rendering them unsuitable for real-time applications in edge computing. To overcome this limitation, we proposed DGS-YOLOv7-Tiny, a lightweight pest detection model based on YOLOv7-Tiny that was specifically optimized for edge computing environments.

A Digital Alert Feedback System (Aged Care Electronic Dashboard Information Tool, ACED-IT) to Enhance Quality Nursing Care: Participatory Action Research.

Aims: Develop and simulate test a digital alert dashboard drawing from existing data to support nurses, care workers and managers in residential aged care.

Design: Participatory action research, co-designing for an Australian 64-bed residential site.

Methods: Qualitative data were collected through focus groups and analysed using reflective thematic analysis.

An efficient approach for the extraction of glycoproteins from American ginseng by natural deep eutectic solvent.

Glycoproteins have not been reported in the previous studies of American ginseng. This study focuses on developing an efficient method for the extraction of glycoproteins from American ginseng (GAG). Three choline chloride (ChCl)-based natural deep eutectic solvents (NADES) and three extraction techniques including microwave-, ultrasound-, and heating and stirring-assisted extraction were evaluated in the study.

Prevalence of Agitation and Depression Amongst People with Dementia in Australian Residential Aged Facilities: A Cross-Sectional Study Using LLMs.

This preliminary study investigates the prevalence of agitation and depression among dementia residents in Australian Residential Aged Care Facilities (RACFs), utilizing Llama 3.1-8B. Analysis of 9,658 nursing notes from 40 RACFs highlighted significant sex- and age-specific differences and symptom cooccurrence trends.

Leveraging Retrieval Augmented Generation-Driven Large Language Models to Extract Dementia Agitation Symptoms and Triggers from Free-Text Nursing Notes.

Unstructured electronic health records are a rich source of patient-specific information but are challenging for analysis due to inconsistent terminology, diverse data formats, and extensive free-text content. To address this, we developed a named entity recognition model leveraging retrieval-augmented generation (RAG) powered by generative artificial intelligence. The model identifies symptoms and triggers of agitation in dementia from nursing notes within residential aged care facilities (RACFs).

Pilot Application of a Large Language Model to Identify Hospitalisation from Unstructured Electronic Health Records in Residential Aged Care Facilities.

Older people in residential aged care facilities (RACFs) visit hospitals and utilise healthcare services more often than others in the community. Trends in hospitalization rates are essential for designing targeted aged care interventions to reduce preventable hospitalizations and optimize resource use. Unstructured free-text nursing notes in Australian RACFs provide updated health information but require significant manual annotation due to their lack of standardization for supervised learning applications.

Designing an Ontology for a Smart Learning Health System Framework.

The concept of a "Learning Health System Framework" has been defined in various forms, making it difficult for healthcare leaders to adopt an appropriate framework for organisational transformation. There is a pressing need to standardise its components and relationships. Limitations of the existing LHS frameworks include their lack of flexibility to adapt to sociotechnical changes and inability to guide the automatic evaluation initiative on health organisational performance.

A Chemically Tailorable Multifunctional Protein Platform for Glutamatergic Neuron Targeting in Mouse Brain.

Targeting glutamatergic neurons is critical for understanding brain functions and treating neurological disorders linked to glutamate dysregulation. However, existing methods face challenges including low specificity, slow kinetics, poor blood-brain barrier (BBB) penetration, and reliance on transgenic pathways. Here, we report the cyanobacterial ferredoxin-dependent glutamate synthase (Fd-GOGAT) as a non-transgenic, multifunctional platform for rapid and selective targeting of glutamatergic neurons in vivo.

High Co-Expression of and as a Predictor of Platinum Resistance and Poor Prognosis in Patients with Epithelial Ovarian Cancer.

This study aimed to determine whether the expression levels of and are associated with survival outcomes and platinum resistance in epithelial ovarian cancer (EOC) patients. We analyzed the medical records of EOC patients. EOC samples obtained during surgery were stained for and .

Receptor-Mediated SPION Labeling of CD4 T Cells for Longitudinal MRI Tracking of Distribution Following Systemic Injection in Mouse.

Tracking T cells in vivo using MRI is a major challenge due to the difficulty of labeling these non-phagocytic cells with a sufficient contrast agent to generate a detectable signal change. In this study, we explored CD4-Superparamagnetic iron oxide nanoparticles (SPION), which is commonly used in magnetic cell sorting, as a potential receptor-mediated, specific CD4 T cell MRI labeling agent. We optimized the labeling protocol for maximal CD4 cell labeling and viability.

Sustained high Life's Essential 8 is associated with lower risk of cerebral small vessel disease: a community-based study.

Objective: We aimed to investigate the influence of Life's Essential 8 (LE8) score trajectories on cerebral small vessel disease (CSVD).

Methods: A total of 1,162 subjects from the Kailuan prospective cohort study were enrolled. The LE8 score trajectories were developed from 2006 to 2021 using trajectory modeling of the SAS procedure Proc Traj.

Halogen-Driven Ion Transport Homogenization in 3D Hierarchical MOF for Ultrastable Solid-State Lithium Metal Batteries.

Solid-state lithium metal batteries (SSLMBs) are hindered by limited ionic conductivity, heterogeneous lithium flux and interfacial instability of solid-state electrolytes. Herein, we report a hierarchical ion-transport network formed by confining lithium halides (LiX, X═Cl, Br, I) within the mesoporous cages of MIL-100(Al), synergistically integrated with a PVDF-HFP polymer matrix. The 3D interconnected pores (0.

A multimodal imaging approach for imaging the metabolic changes resulting from bronchopulmonary dysplasia.

Lung tissue is composed of various functional units, each essential for maintaining the intricate functions of the lung. Disruptions in the molecular and cellular mechanisms in the lung can cause tissue fibrosis, inflammation, and severe breathing difficulties, which are common in conditions such as bronchopulmonary dysplasia (BPD). BPD's molecular changes are not well understood, which hinders effective diagnosis and treatment.

Metal/Insulator/Metal Sandwich Structure Improves Background Potential Stability of Galvanic Redox Potentiometry.

Galvanic redox potentiometry (GRP) has been used in various research fields, particularly for neurotransmitter detection. However, background potential fluctuations significantly limit its application for detecting basal neurotransmitter levels in real samples. Herein, we demonstrate that a metal/insulator/metal sandwich structure substantially improves the GRP background potential stability.

Steric effect of intermediates induces formation of three-dimensional covalent organic frameworks.

Constructing three-dimensional covalent organic frameworks (3D COFs) remains a significant challenge compared to other areas of reticular chemistry. Most existing methods for constructing 3D COFs emphasize the necessity of at least one precursor being extended into 3D space. As a result, designing and synthesizing precursors with specific stereoconformations has become a dominant strategy for discovering new 3D COFs.

HMGB1 inhibits the IFN-γ-induced PD-L1 expression in NSCLC.

T cells are the most important cytotoxic cells involved in antitumor immune responses. After recognizing the major histocompatibility complex (MHC)-peptide complex, cytokines including interferon-γ (IFN-γ) are released to kill tumor cells. However, IFN-γ can also induce tumor cells to express PD-L1.

Edible Safety Evaluation of Seed Kernel Oil: Sub-Chronic Toxicity and Teratogenicity Assessments.

Medium chain triglycerides (MCTs) are regarded as an important ingredient for functional foods and nutraceuticals. seed kernel oil (CCSKO) contains more than 95% medium chain fatty acids (MCFAs), which is a significantly higher level than palm kernel oil (62%) and coconut oil (55%). However, the safety assessment of CCSKO, as the only natural MCT oil rich in capric acid and lauric acid found so far in the world, has not been fully verified.

Chinese Bioethics of Trust and Autonomy: How Doctors Make Patient Referrals After the Introduction of a Two-Way Referral System.

Based on 24 semi-structured interviews with secondary hospital doctors, we empirically investigate how a new two-way referral system in China influences the alignment between referring doctors, patients, and doctors who receive referrals. In particular, we focus on how this system addresses the challenges of trust, autonomy, and bioethics during the shared decision-making process. Our research shows that the two-way referral system has spawned new acquaintance networks, therefore improving communication and collaboration between doctors.