Chromosome-level haplotype-resolved genome assembly of the giant honeycomb oyster, Hyotissa hyotis.

The giant honeycomb oyster, Hyotissa hyotis, a common bivalve inhabitant of tropical and subtropical coastal waters, holds significant ecological and economic importance due to its shell characteristics, rapid growth, and high-quality adductor muscle. However, the lack of high-quality genome has impeded the genetic study and artificial breeding of this species. In this study, we provided the first chromosomal-level haplotype-resolved assembly for the H.

Steric effect of intermediates induces formation of three-dimensional covalent organic frameworks.

Constructing three-dimensional covalent organic frameworks (3D COFs) remains a significant challenge compared to other areas of reticular chemistry. Most existing methods for constructing 3D COFs emphasize the necessity of at least one precursor being extended into 3D space. As a result, designing and synthesizing precursors with specific stereoconformations has become a dominant strategy for discovering new 3D COFs.

From Charge Storage Rulebook Rewriting to Commercial Viability of Zinc-Manganese Batteries.

Aqueous zinc-manganese oxide (Zn-MNO) batteries represent a compelling solution for grid-scale energy storage due to their inherent safety, cost-effectiveness and ecological compatibility. However, the commercialization of this technology faces critical challenges including insufficient electrode durability, limited areal capacity output, and fundamentally ambiguous charge storage principles, which collectively hinder practical implementation. Through systematic mechanistic investigation, a previously overlooked phase evolution paradigm is revealed.

Genetic Basis of Low-Salinity Tolerance in the Pacific Oyster (Crassostrea gigas) as Revealed by Estimation of Genetic Parameters and Genome-Wide Association Study.

The Pacific oyster (Crassostrea gigas), a species of significant economic importance in global aquaculture, faces increasing challenges due to climate change and salinity fluctuations in coastal environments. This study aims to explore the breeding potential of low salinity tolerance traits and dissect their genetic basis, thereby improving environmental adaptability and expanding aquaculture zones. A total of 845 oysters from 36 full families were exposed to a low-salinity challenge (10 practical salinity units) for assessing phenotypic variation, estimating genetic parameters, and dissecting the genetic basis of low-salinity tolerance.

Heating and Cooling Electrochemical 4D-STEM Probing Nanoscale Dynamics at Solid-Liquid Interfaces.

/ methods have revolutionized our fundamental understanding of molecular and structural changes at solid-liquid interfaces and enabled the vision of "watching chemistry in action". transmission electron microscopy (TEM) emerges as a powerful tool to interrogate time-resolved nanoscale dynamics, which involve local electrical fields and charge transfer kinetics distinctly different from those of their bulk counterparts. Despite early reports on electrochemical or heating liquid-cell TEM, developing TEM with simultaneous electrochemical and thermal control remains a formidable challenge.

Chromosomal Fusions Reshape Gene Expression and Environmental Responses in Corbicula Clams.

Genome-structural evolution on a large scale is prevalent in various organisms. The role of genome-structural rearrangements, including chromosome fusions, has been revealed in genome evolution and species divergence. However, the empirical evidence in adaptation remains debated at the current time.

Haplotype-resolved genomes provide insights into the origins and functional significance of genome diversity in bivalves.

Bivalves are famed for exhibiting vast genetic diversity of poorly understood origins and functional significance. Through comparative genomics, we demonstrate that high genetic diversity in these invertebrates is not directly linked to genome size. Using oysters as a representative clade, we show that despite genome size reduction during evolution, these bivalves maintain remarkable genetic variability.

Regulatory variants of glycogen synthase contribute to differential glycogen content between Crassostrea gigas and C. sikamea.

In marine bivalves, glycogen functions as a key energy source, influencing essential physiological processes such as growth, development, gametogenesis, and the reproductive cycle. Glycogen synthase (GYS), the key enzyme regulating glycogen biosynthesis, shows expression patterns directly correlated with seasonal glycogen fluctuations. This study identified genetic variations within the transcriptional regulatory region of the GYS gene in populations of Crassostrea gigas and C.

DOPA Decarboxylase (DDC) in Pacific Oysters: Characterization and Role in Tyrosine Metabolism and Melanogenesis.

DOPA decarboxylase (DDC) plays a crucial role in the physiological functions of animals by participating in the dopaminergic system. However, the functions of DDC in shellfish remain poorly understood. The Pacific oyster (Crassostrea gigas) is an extensively cultivated shellfish.

Crystal-Facet-Controlled Internal Electric Field in MOF/COF Heterojunction Towards Efficient Photocatalytic Overall Water Splitting.

Covalently integrating two type of crystalline porous materials, metal-organic frameworks (MOFs) and covalent organic frameworks (COFs), to form heterostructure photocatalysts inheriting their structural merits has shown inherent advantages in hydrogen evolution reaction. However, how to control the internal electric field in aimed MOF/COF heterojunction to achieve an improved photocatalytic activity is still ambiguous. Herein, for the first time, we report a rational control of the internal electric field in MOF/COF heterojunction by engineering the crystal facet of MOFs to achieve enhanced photocatalytic overall water splitting (OWS) activity.

CgPPOX and CgFECH Mediate Protoporphyrin IX Accumulation Predominantly in the Outer Mantle Fold of Pacific Oyster Crassostrea gigas.

Mollusk shell is secreted through mantle folds and exhibits color polymorphism predominantly caused by pigments. Mantle tissue consists of three folds, with the outer fold playing crucial roles in shell formation. Among various pigments influencing shell color, porphyrins-especially protoporphyrin IX (PPIX)-significantly contribute to orange-shell phenotype.

Anode-Free Zinc-Bromine Batteries Enabled by a Simple Prenucleation Strategy.

The design of anode-free zinc (Zn) batteries with high reversibility at high areal capacity has received significant attention recently, which is quietly challenging yet. Here, a Zn alloyed interface through electroplating is introduced, providing homogeneous Zn prenucleation sites to stabilize subsequent Zn nucleation and plating. By employing Zn-Cu alloy as a module, the complementary simulations and characterizations confirm that the prenucleation alloyed interfaces achieve a homogeneous electric field distribution and greatly enhance the stability of the Zn anode.

DNA Methylation of Somatic Tissues in Oysters is Influenced by Sex and Heredity.

The influence of sex and heredity on DNA methylation in the somatic tissues of mice has been well-documented, with similar hereditary effects reported in honeybees. However, the extent to which these factors affect DNA methylation in molluscan somatic tissues remains poorly understood. In this study, we investigated genomic DNA methylation patterns in the adductor muscle of two genetically distinct oyster strains using whole-genome bisulfite sequencing (WGBS).

SNP Fingerprinting for Germplasm Identification of the Fast-Growing Pacific oyster (Crassostrea gigas) "Haida No. 1" Variety.

The Pacific oyster (Crassostrea gigas) is a global aquaculture species of economic significance. Selective breeding programs have been conducted to produce multiple strains with fast growth as well as other desirable traits. However, due to the phenotypic plasticity of oysters, challenges existed for precise germplasm identification among selectively bred strains.

Comprehensive identification of a disulfidptosis-associated long non-coding RNA signature to predict the prognosis and treatment options in ovarian cancer.

Purpose: Distinguished from cuproptosis and ferroptosis, disulfidptosis has been described as a newly discovered form of non-programmed cell death tightly associated with glucose metabolism. However, the prognostic profile of disulfidptosis-related lncRNAs (DRLRs) in ovarian cancer (OC) and their biological mechanisms need to be further elucidated.

Materials And Methods: First, we downloaded the profiles of RNA transcriptome, clinical information for OC patients from the TCGA database.

The value of multiparameter MRI of early cervical cancer combined with SCC-Ag in predicting its pelvic lymph node metastasis.

Purpose: To investigate the value of multiparameter MRI of early cervical cancer (ECC) combined with pre-treatment serum squamous cell carcinoma antigen (SCC-Ag) in predicting its pelvic lymph node metastasis (PLNM).

Material And Methods: 115 patients with pathologically confirmed FIGO IB1~IIA2 cervical cancer were retrospectively included and divided into the PLNM group and the non-PLNM group according to pathological results. Quantitative parameters of the primary tumor include K, K, V from dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), ADC, ADC, ADC, D, D and f from intravoxel incoherent motion diffusion-weighted imaging (IVIM-DWI) were measured.

Synthesis of iron phthalocyanine/CeO Z-scheme nanocomposites as efficient photocatalysts for degradation of 2,4-dichlorophenol.

Modulating the photogenerated electrons of CeO to activate O and efficiently photocatalytic degradation of chlorophenols is a highly desired goal. Herein, we have successfully fabricated an FePc/CeO heterojunction through H-bond induced assembly. The photocatalytic degradation of 2,4-DCP by the amount-optimized FePc/CeO nanocomposite was improved by 3 times compared with that by pure CeO.

Characterization of piRNAs in Diploid and Triploid Pacific Oyster Gonads: Exploring Their Potential Roles in Triploid Sterility.

PIWI-interacting RNAs (piRNAs) are crucial for silencing transposable elements, germ cell development, and gametogenesis. Triploid Pacific oysters (Crassostrea gigas) are vital in the oyster aquaculture industry due to reduced fertility and rapid growth. This study integrates piRNA and mRNA expression analyses to elucidate their potential contributions to the sterility of triploid C.

Whole-genome DNA methylation profiling revealed epigenetic regulation of NF-κB signaling pathway involved in response to Vibrio alginolyticus infection in the Pacific oyster, Crassostrea gigas.

DNA methylation, an essential epigenetic alteration, is tightly linked to a variety of biological processes, such as immune response. To identify the epigenetic regulatory mechanism in Pacific oyster (Crassostrea gigas), whole-genome bisulfite sequencing (WGBS) was conducted on C. gigas at 0 h, 6 h, and 48 h after infection with Vibrio alginolyticus.

TU-LESS procedure for acute abdomen in late pregnancy: a retrospective study.

Background: Acute abdominal conditions during pregnancy are significant risks to maternal and fetal health, necessitating timely diagnosis and intervention. The choice of surgical approach is a major concern for obstetricians.

Objective: To evaluate the safety and efficacy of the TU-LESS procedure for acute abdomen in late pregnancy.

Revealing the structural evolution of CuAg composites during electrochemical carbon monoxide reduction.

Comprehending the catalyst structural evolution during the electrocatalytic process is crucial for establishing robust structure/performance correlations for future catalysts design. Herein, we interrogate the structural evolution of a promising Cu-Ag oxide catalyst precursor during electrochemical carbon monoxide reduction. By using extensive in situ and ex situ characterization techniques, we reveal that the homogenous oxide precursors undergo a transformation to a bimetallic composite consisting of small Ag nanoparticles enveloped by thin layers of amorphous Cu.