Application of Fe-CuO bimetallic oxide nanozyme-based colorimetric sensing for highly sensitive detection of tetracycline: theoretical and experimental studies.

This research establishes a competitive colorimetric detection platform for tetracycline (TC) residue analysis in milk and aqueous matrices, with implications for assessing antibiotic-derived health risks in dairy and potable water systems. According to the developed colorimetric competitive reaction model, steady-state kinetic analysis revealed that the Fe-doped CuO (Fe-CuO) nanozymes exhibit higher substrate affinity and faster reaction kinetics than conventional enzymes. The catalytic system utilized a bimetallic nanozyme composed of Fe-CuO, which exhibited enhanced catalytic performance, using 3,3',5,5'-tetramethylbenzidine (TMB) as the color-changing substrate.

Current landscape of the immunoproteasome: implications for disease and therapy.

The immunoproteasome, an inflammation-induced proteasome variant, coordinates proteostasis and adaptive immunity by replacing constitutive subunits (β1, β2, β5) with inducible counterparts (β1i, β2i, β5i). This specialization enhances antigen processing for MHC class I presentation and oxidative protein clearance. Beyond immune regulation, it critically contributes to cardiovascular, respiratory, neurodegenerative, autoimmune, retinal, and oncological pathologies through mechanisms involving NF-κB activation, mitochondrial dysfunction, and inflammatory polarization.

Clinicopathological Features and Pathogenesis of Thymoma Complicated with Alopecia Areata: A Multicenter, Matched Case Analysis.

Background: Thymoma is a malignant tumor originating from the thymic epithelium and can be associated with over 100 paraneoplastic syndromes (PNSs). Due to the low incidence of thymoma and the relative rarity of alopecia areata (AA) as an associated autoimmune disease, patients with thymoma combined with AA are relatively uncommon in clinical practice. As a result, the clinicopathological features and pathogenesis of such patients have been rarely investigated.

Translocator protein deficiency blocks the ferroptosis of malignant peripheral nerve sheath tumors through glutathione peroxidase 4.

Background: Malignant peripheral nerve sheath tumor (MPNST) is an aggressive soft tissue sarcoma characterized by high recurrence and poor prognosis, necessitating the search for novel therapeutic targets and strategies. This study investigated the expression of mitochondrial translocator protein (TSPO) in MPNST and its role in regulating ferroptosis.

Methods: TSPO expression was analyzed in adjacent non-tumor tissues, benign neurofibromas, and malignant tissues using real-time PCR, western blotting, immunohistochemistry staining.

Outcomes of isolated mitral valve surgery performed via right anterolateral thoracotomy: a single-centre experience.

Objectives: The evidence underlying thoracic and pulmonary complications and clinical outcomes after isolated mitral valve surgery performed via right anterolateral thoracotomy is inconclusive. This study retrospectively compared the postoperative thoracic and pulmonary complications and clinical outcomes of isolated mitral valve surgery performed via right anterolateral thoracotomy vs. median sternotomy.

Butein Alleviates Non-Alcoholic Steatohepatitis in Leptin-Deficient Mice by Modulating the PDE4/cAMP/p-CREB Pathway.

Purpose: Non-alcoholic steatohepatitis (NASH) is a prevalent liver disease characterized by steatosis, inflammation, and liver injury. Despite its increasing incidence, effective treatments are limited. Butein, a flavonoid with anti-cancer, anti-inflammatory, and antioxidant properties, has not been thoroughly studied for its potential therapeutic effects in NASH.

Polydopamine-engineered FRET microspheres for robust dual-readout multiplexed nucleic acid analysis.

We present a polydopamine-engineered FRET-encoded microsphere platform that ensures stable spectral encoding, efficient decoding, improved dispersion, and reproducible high-density DNA immobilization (∼0.1 nmol mg), enabling robust and sensitive multiplexed nucleic acid analysis with low background signals.

Wedelolactone Ameliorates Ischemic Stroke by Inhibiting Oxidative Damage and Ferroptosis via HIF-1α/SLC7A11/GPX4 Signaling.

Purpose: Wedelolactone (Wel), a furocoumarin compound extracted from the medicinal plant . has been shown to exhibit significant neuroprotective effects. However, the potential of Wel to improve ischemic stroke (IS) and the underlying mechanisms remain unclear.

Array-Based Rapid Identification of Herbal Medicines via a Bifunctional Color-BiLink Strategy.

Ensuring the authenticity and quality of herbal medicines, particularly licorice, a pivotal component in nearly 60% of traditional Chinese medicine formulations, remains a significant global challenge. However, conventional approaches lack the capacity for direct, rapid, and high-throughput differentiation of licorice mixtures and structurally analogous ingredients. Herein, we present a multisite-binding Color-BiLink () strategy for constructing the dual-mode responsive sensor array, formed by a quantifiable-recognized bifunctional -nitrophenylboronic acid-modified PEI () and four dyes through dynamic, reversible covalent, and electrostatic interactions.

Echinacoside targets the HIF-1α/LDHA axis to modulate A1/A2 astrocyte differentiation and promote glial scar repair following ischemic stroke.

Ethnopharmacological Relevance: Glial scar repair is an emerging approach to ischemic stroke (IS) management, and this process can be both positively and negatively influenced by astrocytes. As a phenylethanoid glycoside derived from Cistanche tubulosa (Schenk) Wight, echinacoside (ECH) exhibits an array of pharmacological effects. However, there has been little research specifically focused on the impact of ECH on glial scar repair following IS.

Mitochondria: a key regulator of programmed cell death in OP.

Osteoporosis (OP) is a common clinical systemic bone disease, with insidious onset and usually causes serious complications such as fractures. Studies have found that the dysfunction of a variety of bone cells will lead to enhanced bone resorption and reduced bone formation capacity, thus resulting in the imbalance of bone homeostasis and OP disease. As a class of regulatory death mode that affects cell function, programmed cell death (PCD) has been proved to play an important role in maintaining various bone cells growth activities and maintaining bone homeostasis.

Causal Relationship Between Matrix Metalloproteinase with Their Tissue Inhibitors and Human Immunodeficiency Virus Infection: A Two-Sample Bidirectional Mendelian Randomization Analysis.

Studies have shown an association between matrix metalloproteinases (MMPs) along with tissue inhibitors of MMPs (TIMP) and human immunodeficiency virus (HIV) infection and CD4 T cell count, a key clinical indicator for HIV progression, but the causality remains unclear. This study aimed to investigate the bidirectional causal relationship between MMPs/TIMP and HIV. A genome-wide association study-based two-sample bidirectional Mendelian randomization (MR) analysis was conducted to elucidate the potential causal links between MMPs/TIMP and HIV.

Organic Carbon Sequestration by Secondary Fe-Mn Complex Minerals via the Anoxic Redox Reaction of Fe(II) and Birnessite.

Organic matter (OM) sequestrated by iron (Fe) and manganese (Mn) complex minerals may be an effective way to achieve organic carbon (OC) persistence in sediments and soils. However, the impact of Fe-Mn complex minerals from a redox reaction of Fe(II) and birnessite on OC dynamics remains unexplored. Herein, the immobilization and molecular transformation of fulvic acid (FA), mediated by a redox reaction of Fe(II) and birnessite, were investigated at pH 4.

Rosmarinic acid attenuates blood-brain barrier dysfunction to improve cerebral ischemia/reperfusion injury in mice.

Background: Cerebral ischemia/reperfusion (CI/R) has detrimental effects in cases of ischemic stroke. This notably triggers the blood-brain-barrier (BBB) to break. Known for its ability to provide neuroprotective benefits, rosmarinic acid (RosA) is a phenolic acid compound in the Lamiaceae family of plants.

MRD status combined with TNM staging optimizes postoperative prognostic stratification in non-small cell lung cancer: a meta-analysis and systematic review.

Objective: To investigate survival differences based on MRD status at different landmarks and tumor staging in patients with early-stage non-small cell lung cancer (NSCLC) following surgical resection.

Methods: Data from six studies including 867 early-stage NSCLC patients who underwent surgery were summarized. Patients were grouped according to MRD status at different detection time points and tumor staging.

Impact of Pandemic Shocks on Participation in Voluntary Organizations Among Older Adults in Nine Countries.

Social engagement, including participation in voluntary organizations, is vital for healthy aging. This study investigated the impact of the global pandemic on the participation of older adults aged 60 years and above in 10 types of voluntary organizations. Utilizing detailed COVID-19 data from the World Health Organization and the Integrated Values Surveys across 9 countries that collected data on voluntary organization participation before and during the pandemic, this study employed Difference-in-Difference models to estimate the pandemic's effect on the likelihood of older adults participating in voluntary services.

Ribosome Engineering for Enhanced Butenyl-Spinosyn Production in Saccharopolyspora pogona.

Ribosome engineering, a strategy that utilizes antibiotic resistance mutations to modulate ribosomal function, has emerged as a powerful approach for enhancing microbial metabolite production. In this study, ribosome engineering was applied to Saccharopolyspora pogona ASAGF2-G4 under streptomycin selection to improve butenyl-spinosyn production. Screening for streptomycin-resistant mutants at concentrations ranging from 2 to 20 µg/mL resulted in the isolation of 58 mutants, of which 27.

In Situ Quantification of Surface pH of Dry Soils and Minerals: An Innovative Approach Combining Indicators and Diffuse Reflectance Spectroscopy.

Most natural soils and minerals are typically dry or semidry, and their surface characteristics and interfacial interactions are distinct from those of slurry systems. The surface acidity of dry soils and minerals cannot be measured by using traditional methods such as potentiometric and temperature-programmed desorption methods. In this article, we describe the development of an analytical method based on a combination of acid-base indicators and diffuse reflectance spectroscopy (DRS).

Cystic adventitial disease of the popliteal artery in female: case report and literature review of a rare differential diagnose of artery stenosis.

Background: Cystic adventitial disease (CAD) is a rare vascular condition that causes arterial stenosis due to the presence of a cyst in the adventitia (outer layer) of the artery. It is most commonly seen in young and middle-aged men, with an occurrence rate of around 0.1% among vascular diseases.

Molecular mechanisms of s-methoprene-induced growth inhibition in Ephestia elutella (Hübner) (Lepidoptera: Pyralidae): insights from transcriptomic analysis.

Ephestia elutella is a globally distributed storage pest, and its growth and development are regulated by juvenile hormones. To investigate the molecular mechanisms underlying the response of E. elutella larvae to the juvenile hormone analog s-methoprene, this study examined the effects of s-methoprene on the growth and development of E.

Military experience and depression: a prospective multi-cohort analysis across nations.

Depression is a growing public concern, but whether it is associated with military experience still needs investigation. This study aimed to examine this association among male veterans. We employed data from the Chinese Family Panel Study 2010-2020 (China), the Socio-Economic Panel 2009-2021 (Germany) and the Panel Study of Income Dynamics 2009-2021 (USA) and included 27300 male adults aged above 18 years.

Age Disparity in the Association Between Internet Use and Trust in Doctors Among Chinese Adults.

Purpose: To examine associations between internet use behaviors, network information literacy, and trust in doctors across age groups, and to test the mediating role of subjective well-being (SWB).

Method: Multiple linear regression was used to test associations between internet use behaviors, network information literacy, and trust in doctors. The mediation effect of SWB was tested with the Karlson-Holm-Breen method.

miR-210 Regulates Autophagy Through the AMPK/mTOR Signaling Pathway, Reduces Neuronal Cell Death and Inflammatory Responses, and Enhances Functional Recovery Following Cerebral Hemorrhage in Mice.

Recently, a growing body of research has shown that microRNAs (miRNAs) are crucial in the pathophysiological mechanisms of brain disorders, miR-210 is one of the significant miRNAs implicated in these disorders, and its function in intracerebral hemorrhage (ICH) is not yet fully understood. Research the impact of miR-210 on intracerebral hemorrhage and probe into its working mechanism. The ICH model was established by injecting collagenase into the basal ganglia of male C57/BL6 mice (n = 142).

Presentation, treatment, and outcomes in patients with spontaneous isolated celiac artery dissection.

Spontaneous isolated celiac artery dissection (SICAD) is a rare condition, with its natural history and outcomes have not been thoroughly studied. The aim of this study is to review our institution's experience in evaluating, treating, and determining outcomes for patients with SICAD. A retrospective review was conducted on 76 patients who presented with SICAD between January 2010 and July 2022.