Active Vitamin D Corrects Cerebrovascular Dysfunction and Aberrant Vasopressin Expression in the Hypertension Phenotype of 1α-Hydroxylase Knockout Mice.

Vitamin D has a protective effect on the brain under hypertensive conditions. Studies have shown that 1,25-dihydroxyvitamin D (1,25(OH)D) can negatively regulate hypertension and central renin-angiotensin system activation through a central anti-oxidative mechanism in 1α-hydroxylase knockout mice. To confirm whether endogenous or exogenous 1,25(OH)D deficiency or supplementation alters cerebrovascular function and vasopressin expression through anti-oxidation, researchers provided 1α(OH)ase mice and their wild-type littermates with regular diet, a high-calcium, high-phosphorus rescue diet with N-acetyl-L-cysteine supplementation, or 1,25(OH)D subcutaneous injection.

Demand analysis of perioperative nursing service needs for patients with cervical spondylosis based on the Kano model: a cross-sectional study.

Background: This study uses the Kano model as its theoretical foundation to classify the needs of patients with cervical spondylosis according to their perioperative nursing service needs. It defines, prioritizes, and categorizes these needs to identify the core requirements of perioperative nursing services for patients with cervical spondylosis. The aims of this study are to provide high-quality medical and nursing services during the perioperative period for patients with cervical spondylosis and to offer evidence for improving patient satisfaction.

The relationship between illness perception and quality of life among Chinese rural elderly patients with hypertension: the mediating role of medication experience.

Objective: A significant correlation has been discovered between illness perception and quality of life, but the relationships among illness perception, medication experience, and quality of life remain to be confirmed. Therefore, this study aims to identify the relationship between illness perception and quality of life among Chinese rural elderly patients with hypertension and to analyse the possible mediating role of medication experience between these two aspects.

Methods: From September to December 2023, a cross-sectional study was conducted to select rural elderly patients with hypertension by multi-stage stratified random sampling.

[Pathophysiological mechanisms linking chronic stress and cervical spondylosis of vertebral artery type: A theoretical framework of the neuroendocrine-immune network].

Stress is a critical inducer in the onset and progression of many chronic diseases. Prolonged or intense stress can disrupt the overall balance between the nervous, immune, and endocrine systems. The resulting biological signals may act on corresponding receptors in the cervical spine region, leading to adverse pathological changes.

Flavoromic analysis of dynamic changes and metabolic pathways of flavor compounds in Shai vinegar across aging stages and types.

To better understand the flavor evolution of Shai vinegar (SV) during aging, we systematically characterized the volatile profiles of SV samples using a flavoromics approach. Volatile organic compounds (VOCs) were analyzed based on their relative odor activity values and odor activity values (OAVs). Citric acid was detected for the first time at concentrations comparable to those of lactic acid and acetic acid.

Relationship Between Short Video Addiction Tendency and Depression Among Rural Older Adults: Cross-Sectional Study.

Background: Depression, a prevalent mental illness among older people, is associated with some adverse health problems and lower quality of life. Against the backdrop of a growing aging population, coping with late-life depression has become an important public health priority. Emerging evidence suggests that short video addiction tendency may be a new risk factor for depression.

Long Non-coding RNA DANCR Regulates the Proliferation and Migration of Human Adipose-derived Mesenchymal Stromal Cells in Inflammation Conditions.

Background: Mesenchymal stromal cells (MSCs) and Dexamethasone (Dex) are both effective methods to treat inflammatory diseases. However, the interaction between inflammatory factors, Dex, and MSCs in repair is not fully understood. The purpose of this study is to clarify the effects and mechanisms of glucocorticoids on the tissue repair characteristics of MSCs in an inflammatory environment.

Characterization of the Quality and Flavor in Chinese Sausage: Comparison Between Cantonese, Five-Spice, and Mala Sausages.

With the increasing popularity of traditional Chinese sausages both domestically and internationally, the flavor characteristics of sausages have become an important topic in food science research. However, comparative studies on the flavor differences between different types of traditional Chinese sausages are still limited. This study aimed to systematically compare the flavor profiles of three representative types of traditional Chinese sausages (Cantonese, Five-Spice, and Mala sausages), with 20 samples randomly selected from one batch of 100 sausages per type produced in December 2024, using a combination of headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS), amino acid analysis, electronic sensory analysis, and sensory evaluation techniques.

Dual-Active Nanoimmunomodulators for the Synergistic Enhancement of the Antitumor Efficacy of Photodynamic Immunotherapy.

Photodynamic immunotherapy, which combines photodynamic therapy (PDT) with immunotherapy, has become an important and effective treatment for cancer. However, most photodynamic immunotherapy systems for cancer do not allow for the precise release of immunomodulators, leading to systemic side effects and poor patient prognosis. This study reports a dual-activatable nanoimmunomodulator (CPPM), whose photodynamic effect and agonist release are both activated in response to specific stimuli, which can be used for precise photodynamic immunotherapy of cancer.

The association between genetic polymorphisms in GCKR and gout: A systematic review and meta-analysis.

Background: As far as we know, there are no studies summarizing single nucleotide polymorphism (SNP) in the GCKR gene associated with gout, and the association of SNP in the GCKR gene with gout is controversial.

Methods: Studies were obtained from PubMed, Embase, Web of Science, and Cochrane Library, last searched on February 20, 2024. We reviewed the reported SNP loci of the GCKR gene associated with gout.

Association between Human Milk Fatty Acid Esters of Hydroxy Fatty Acids and Maternal Body Mass Index and Early Infant Growth: A Longitudinal Study.

Background: Fatty acid esters of hydroxy fatty acids (FAHFAs) are a novel class of endogenous lipids with biologically active functions in human.

Objectives: Our objective was to determine the role of maternal body mass index (BMI) in breast milk (BM) FAHFA composition and delineate the association between these BM fatty acids and infant growth during the first 6 mo postpartum.

Methods: We recruited 65 healthy singleton pregnant females from the obstetric clinic.

Green synthesis of paulownia flower-derived carbon dots for sensitive fluorescent detection of paclobutrazol.

In this study, carbon dots derived from paulownia flowers (PF-CDs) were synthesized and utilized as a nanosensor for the highly sensitive detection of paclobutrazol (PBZ) pesticide residue. The study reports the first green synthesis of PF-CDs using biological waste from PF through a simple one-step hydrothermal method. PF-CDs exhibited exceptional photostability, low cytotoxicity, and excellent biocompatibility, making them suitable for biological applications.

A synthetic peptide, derived from neurotoxin GsMTx4, acts as a non-opioid analgesic to alleviate mechanical and neuropathic pain through the TRPV4 channel.

Mechanical pain is one of the most common causes of clinical pain, but there remains a lack of effective treatment for debilitating mechanical and chronic forms of neuropathic pain. Recently, neurotoxin GsMTx4, a selective mechanosensitive (MS) channel inhibitor, has been found to be effective, while the underlying mechanism remains elusive. Here, with multiple rodent pain models, we demonstrated that a GsMTx4-based 17-residue peptide, which we call P10581, was able to reduce mechanical hyperalgesia and neuropathic pain.

Development of a brain-penetrant G9a methylase inhibitor to target Alzheimer's disease-associated proteopathology.

Current Aβ-targeting therapeutics for Alzheimer's disease (AD) only slow cognitive decline due to poor understanding of AD pathogenesis. Here we describe a mechanism of AD pathogenesis in which the histone methyltransferase G9a noncanonically regulates translation of hippocampal proteins associated with AD pathology. Correspondingly, we developed a brain-penetrant inhibitor of G9a, MS1262, which restored both age-related learning & memory and noncognitive functions in multiple AD mouse models.

Metabolic Engineering of for the Improved Malonic Acid Production.

Malonic acid (MA) is a high-value chemical with diverse applications in the fields of food, agriculture, medicine, and chemical synthesis. Despite the successful biosynthesis of MA has been performed in , , and , the resulting MA titers remain insufficient for industrial-scale production. In this study, three distinct metabolic pathways were designed and constructed to increase MA production in .

Wu-Mei-Wan promotes ferroptosis in gallbladder cancer through STAT3 negative regulation: An integrated HPLC, proteomics, network pharmacology, and experimental validation study.

Ethnopharmacological Relevance: As a traditional Chinese medicine, Wu-Mei-Wan (WMW) has shown promise as a second-line treatment for gallbladder cancer, but its mechanism remains to be explored.

Aim Of The Study: To determine the specific mechanism of WMW active ingredients (Palmatine et al.) inducing ferroptosis in gallbladder cancer (GBC) and its synergistic potential with gemcitabine.

The relationship among socioeconomic status, social support and frailty: is there a gender difference?

Objective: This study aimed to determine the relationship among socioeconomic status, social support and frailty, and its gender difference.

Methods: Education and income were combined to indicate the socioeconomic status. The Social Support Rating Scale (SSRS) was used to measure the level of social support.

Calcium-activated ion channels drive atypical inhibition in medial habenula neurons.

Nicotine is an addictive substance that poses substantial health and societal challenges. Despite the known links between the medial habenula (MHb) and nicotine avoidance, the ionic mechanisms underlying MHb neuronal responses to nicotine remain unclear. Here, we report that MHb neurons use a long-lasting refractory period (LLRP) as an unconventional inhibitory mechanism to curb hyperexcitability.

The impact of rhizosphere soil microorganisms on the medicinal active ingredients of from different regions.

Aims: Analyzing the rhizosphere microbial community structure of from different regions and its correlation with the accumulation of main medicinal active ingredients, this study aims to explore the impact of rhizosphere soil microorganisms on the effective components of , providing a scientific basis for the high-quality and high-yield cultivation of .

Methods And Results: The rhizosphere soil of three-year-old was used as the research object. High-throughput sequencing technology was employed to analyze the rhizosphere bacterial and fungal community structures.

Facile preparation of fluorescent carbon dots from water caltrop shells and their application in amikacin sensing.

Amikacin (AMK) effectively treats infections from Gram-negative bacilli and penicillin-resistant . However, prolonged administration of AMK may result in adverse effects such as nausea, headache, ototoxicity, and hearing loss, necessitating a reliable detection method. Carbon dots (CDs), known for their excellent optical properties, are a promising fluorescent probe.

Exploring the structures and molecular mechanisms of bioactive compounds from marine foods for hyperuricemia prevention: a systematic review.

Hyperuricemia, characterized by an elevation in serum uric acid (UA) levels, stands as a significant metabolic ailment threatening human well-being. Presently, dietary adjustments have become a crucial strategy in managing serum UA levels among individuals grappling with hyperuricemia and gout. Given its unique ecosystem, the ocean hosts a plethora of organisms boasting distinct structures and active components.

The Role of HADHB in Mitochondrial Fatty Acid Metabolism During Initiation of Metastasis in ccRCC.

The initiation and progression of clear cell renal cell carcinoma (ccRCC) are closely linked to significant metabolic alterations. Specifically, lipid metabolism alterations and their association with the high invasiveness in ccRCC require further investigation. After conducting RNA-sequencing (RNA-seq), we discovered that Hydroxyacyl-CoA Dehydrogenase Trifunctional Multienzyme Complex Subunit Beta (HADHB) was significantly downregulated in the highly invasive ccRCC cell line.

The formation pathway of flavor compounds in steamed Antarctic krill () based on untargeted metabolomics.

This study investigated the impact of steaming on the flavor and metabolic profile of Antarctic krill, aiming to elucidate the pathways responsible for flavor development and metabolic shifts during processing. HS-SPME-GC-MS identified key volatile compounds, including alcohols, aldehydes, ketones and so on. The results demonstrated a significant increase in nonanal content from 2.

Engineering Yarrowia lipolytica to Enhance the Production of Malonic Acid via Malonyl-CoA Pathway at High Titer.

Malonic acid (MA) is a high-value-added chemical with significant applications in the polymers, pharmaceutical, and food industries. Microbial production of MA presents enzyme inefficiencies, competitive metabolic pathways, and dispersive carbon flux, which collectively limit its biosynthesis. Here, the non-conventional oleaginous yeast Yarrowia lipolytica is genetically engineered to enhance MA production.

Fluorogenic Tetrazine Bioorthogonal Probes for Advanced Application in Bioimaging and Biomedicine.

A variety of bioorthogonal chemical tools have been developed and widely used in the study of biological phenomena in situ. Tetrazine bioorthogonal chemistry exhibits ultrafast reaction kinetics, excellent biocompatibility, and precise optical regulatory capabilities. Fluorogenic tetrazine bioorthogonal probes have achieved particularly diverse applications in bioimaging and disease diagnosis and treatment.