Symbiotic bacteria mediate chemical-insecticide resistance but enhance the efficacy of a biological insecticide in diamondback moth.

Insecticide resistance has been a major challenge for pest management worldwide. Here, we investigated how gut symbiotic bacteria in insects might affect resistance to chemical (organophosphate) and biological (Bacillus thuringiensis) insecticides in different ways to create opportunities for strategic pesticide rotations. Using the diamondback moth (Plutella xylostella) as the target pest, we demonstrated that long-term exposure to chlorpyrifos (an organophosphate insecticide) promotes the proliferation of the gut symbiont Enterococcus mundtii in P.

Integrated Modeling of Composition-Resolved Source Apportionment and Dynamic Projection for Ozone Pollution in Datong.

Growing ozone (O) pollution in industrial cities urgently requires in-depth mechanistic research. This study utilized multi-year observational data from Datong City, China, from 2020 to 2024, integrating time trend diagnostics, correlation dynamics analysis, Environmental Protection Agency Positive Matrix Factorization 5.0 (EPA PMF 5.

Cloning and Preliminary Functional Studies of the Nicotinic Acetylcholine Receptor α6 Subunit in Megalurothrips usitatus (Bagnall).

To investigate the functional role of the nicotinic acetylcholine receptor (nAChR) α6 subunit in Megalurothrips usitatus and its involvement in insecticide resistance, we cloned the nAChR α6 gene (Muα6) using RACE technology and analyzed its spatiotemporal expression patterns via RT-qPCR. Functional studies were further conducted under spinetoram stress. The results showed that Muα6 (GenBank accession: PQ441948) has a full-length sequence of 1569 bp, encoding 523 amino acids with four transmembrane domains (TM1-TM4), six extracellular loops (Loop A-F), and two N-glycosylation sites (Asn100 and Asn144).

Aptamer-Conjugated Magnetic Nanoparticles Integrated with SERS for Multiplex Salmonella Detection.

is a rapidly spreading and widespread zoonotic infectious disease that poses a serious threat to the safety of both poultry and human lives. Therefore, the timely detection of in foods and animals has become an urgent need for food safety. This work describes the construction of an aptamer-based sensor for detection, using FeO magnetic beads and Ag@Au core-shell nanoparticles-embedded 4-mercaptobenzoic acid (4MBA).

A SERS Sensor Prepared via Electrostatic Self-Assembly of TaC@AgNP Nanocomposites for Detection of Ziram.

MXenes are a class of two-dimensional materials exhibiting excellent surface-enhanced Raman scattering (SERS) performance. Currently, the SERS studies of MXenes have been primarily focused on those with MX and MX structural motifs. In order to expand the SERS sensing application based on MXenes, in this paper, a SERS sensor made of TaC@AgNP nanocomposite material was fabricated by electrostatic self-assembly.

The acetylation GLI1 affects arsenical-induced renal fibrosis by mediating the Hedgehog signalling pathway.

Arsenic exposure by drinking water is a key challenge in the field of public health worldwide. Research has found a connection between arsenic exposure and renal fibrosis, yet the mechanisms behind this are still unclear. In this study, rats were exposed to 50 mg/L NaAsO or 200 mg/L dimethylarsinic acid (DMA) via drinking water for 12 weeks, and HK-2 cells were treated with 1 μM NaAsO for 4 weeks.

Dual-strategy LaMnNiO-Nano perovskite catalysts via B-site doping and nanocasting for cost-effective mineralization of hazardous hexachlorobutadiene.

Hexachlorobutadiene (HCBD), an increasingly threatening persistent organic pollutant, has few mineralization disposal methods due to its high chlorination, thus demanding urgent development of efficient and cost-effective catalytic systems. Herein, LaMnNiO-Nano perovskite catalysts were rationally engineered through a dual-strategy combining nanocasting with B-site doping, which exhibited superior oxidation activity toward HCBD. At 250°C and 10 vol% HO, the CO yield of LaMnNiO-Nano reached 637.

Activation of the ROS/CncC Pathway Regulates Cytochrome P450 to Confer Cycloxaprid Tolerance in .

The inducible P450 gene plays a key role in the response of insects to insecticide stress. However, the transcriptional regulatory mechanism of insecticide-inducible detoxification enzyme genes has not been well clarified. In the present study, a P450 gene, , could be induced by cycloxaprid in both susceptible and cycloxaprid-resistant strains.

Intelligent geographical origin traceability of Pu-erh tea based on multispectral feature fusion.

To achieve accurate origin traceability of Pu-erh tea, this study proposes a deep learning method based on multispectral fusion. By collecting Raman and near-infrared spectral data from five major origins, an improved ECA-ResNet network structure was designed, incorporating an optimized channel attention mechanism for adaptive feature extraction and fusion. While maintaining computational efficiency, the network effectively utilizes Raman spectroscopy's sensitivity to molecular skeleton vibrations and near-infrared spectroscopy's responsiveness to functional group characteristics.

The Association Between High-Sensitivity C-Reactive Protein and the Progression of Arteriosclerosis: The Kailuan Study.

Objective: To explore the association between high-sensitivity C-reactive protein (hs-CRP) and the progression of arteriosclerosis.

Methods: Using a prospective cohort study design, 11,577 participants from the Kailuan Study cohort who underwent at least two brachial-ankle pulse wave velocity (baPWV) examinations and met the inclusion criteria were included as the study subjects. Based on baseline hs-CRP levels, they were divided into three groups: hs-CRP <1 mg/L group, 1 mg/L≤hs-CRP≤3 mg/L group, and hs-CRP >3 mg/L group.

Rapid and broad-spectrum detection of salmonella in various environments by magnetic enrichment-surface enhanced Raman spectroscopy.

Rapid, accurate, and broad-spectrum detection of pathogenic bacteria in food is very important and an emerging need for increasing food safety concerns. Here, we present an ultrasensitive Surface-enhanced Raman spectroscopy (SERS) based aptasensor, which consists of core-shell structure (Ag@Au) and magnetic composites (FeO@Au@Ag). 4-mercaptobenzoic acid (4MBA) as Raman reporter was embedded in Ag@Au nanoparticles (Ag@4MBA@Au) based on an internal standard.

Efficacy, Safety, and Cost-Effectiveness Analysis of Ceftazidime-Avibactam versus Polymyxin B in the Treatment of Carbapenem-Resistant Enterobacteriaceae Infections: A Target Trial Emulation.

Introduction: Treatment options for carbapenem-resistant Enterobacteriaceae (CRE) infections are limited, with polymyxin B (PMB) and ceftazidime-avibactam (CZA) being among the available choices. However, research on these options is scarce and significantly heterogeneous. This study aims to analyze the efficacy, safety, and cost-effectiveness of PMB and CZA within a standardized target trial emulation (TTE) framework.

Revolutionizing Escherichia coli detection in real samples with digital SERS aptamer sensor technology.

Aptamer sensors based on surface-enhanced Raman scattering (SERS) technology have demonstrated great potential in the ultrasensitive and rapid detection of Escherichia coli (E. coli). Herein, this paper presents a digital SERS aptamer sensor.

Preparation of high-performance adsorption carbon materials via hydrothermal carbonization of agricultural solid waste-grapevines.

This study uses grapevine agricultural waste to prepare grapevine-derived biobased activated carbon (AGV) through chemical activation with NaOH after hydrothermal carbonization. A comprehensive analysis of the adsorption performance and mechanisms of AGV for methylene blue (MB) was conducted. The results demonstrated that AGV possesses a highly developed reticular pore structure, with a specific surface area of 3230.

Neoadjuvant toripalimab plus nimotuzumab combined with taxol-based chemotherapy in locally advanced penile squamous cell carcinoma.

The conventional neoadjuvant chemotherapy regimen for locally advanced penile squamous cell carcinoma (La-PSCC) has shown moderate response rates and survival benefits. This single-arm, phase II trial (NCT04475016) evaluated a neoadjuvant regimen of four cycles of toripalimab (anti-PD-1 antibody), nimotuzumab (anti-EGFR antibody), and taxol-based chemotherapy (TNT), followed by consolidative surgery. The primary endpoint was the pathological complete response (pCR) rate.

A Raman spectroscopy algorithm based on convolutional neural networks and multilayer perceptrons: qualitative and quantitative analyses of chemical warfare agent simulants.

Rapid and reliable detection of chemical warfare agents (CWAs) is essential for military defense and counter-terrorism operations. Although Raman spectroscopy provides a non-destructive method for on-site detection, existing methods show difficulty in coping with complex spectral overlap and concentration changes when analyzing mixtures containing trace components and highly complex mixtures. Based on the idea of convolutional neural networks and multi-layer perceptrons, this study proposes a qualitative and quantitative analysis algorithm of Raman spectroscopy based on deep learning (RS-MLP).

A-to-I RNA editing of mediates transgenerational wing dimorphism in aphids.

Wing dimorphism is a common phenomenon that plays key roles in the environmental adaptation of aphid; however, the signal transduction in response to environmental cues and the regulation mechanism related to this event remain unknown. Adenosine (A) to inosine (I) RNA editing is a post-transcriptional modification that extends transcriptome variety without altering the genome, playing essential roles in numerous biological and physiological processes. Here, we present a chromosome-level genome assembly of the rose-grain aphid by using PacBio long HiFi reads and Hi-C technology.

Study on the Effects of National Volume-Based Procurement of Chemical Drugs on Chinese Patent Medicines: Lipid-Lowering Drugs as an Example.

Background: Atherosclerotic cardiovascular disease remains the leading cause of death worldwide. This study aims to explore the impact of national volume-based procurement (NVBP) on Chinese patent medicines and provide evidence for improving policies and promoting rational drug use.

Methods: The study was based on data from the China National Health Insurance Agency that spanned January 2019 to December 2020.

Benchmarking Corrosion with Anionic Polarity Index for Stable and Fast Aqueous Batteries Even in Low-Concentration Electrolyte.

Despite aqueous electrolyte endowing batteries with the merits of safe operation, low-cost fabrication, and high ionic conductivity, water-induced corrosion, including spontaneous chemical and electrochemical hydrogen evolution corrosion, adversely affects lifespan and rate capability. There is still a lack of selection criteria for benchmarking corrosion behavior qualitatively. Through theoretical simulation, an anionic polarity index (API) tactic is proposed to resist corrosion by manipulating interfacial and solvated water concomitantly, thus realizing stable and fast Zn aqueous batteries (ZABs).

Efficient detection of foodborne pathogens via SERS and deep learning: An ADMIN-optimized NAS-Unet approach.

Amid the increasing global challenge of foodborne diseases, there is an urgent need for rapid and precise pathogen detection methods. This study innovatively integrates surface-enhanced Raman Spectroscopy (SERS) with deep learning technology to develop an efficient tool for the detection of foodborne pathogens. Utilizing an automated design of mixed networks (ADMIN) strategy, coupled with neural architecture search (NAS) technology, we optimized convolutional neural networks (CNNs) architectures, significantly enhancing SERS data analysis capabilities.

Causal associations between socioeconomic status and prostate cancer using two-sample Mendelian randomization study.

Prostate cancer (PCa) is a common form of malignancy among men. The associations between socioeconomic status (SES) indicators and PCa risks remain incompletely elucidated. Through two-sample Mendelian randomization (MR), this research seeks to assess the causal links between 4 genetically predicted SES indicators-average total household income before tax, the Townsend deprivation index at recruitment, unemployed status and college or university degree in the household-and PCa.

Transcription factor CREB/ATF regulates overexpression of CYP6CY14 conferring resistance to cycloxaprid in Aphis gossypii.

Aphis gossypii Glover as a destructive agricultural pest has evolved resistance to various insecticides. Cycloxaprid is a novel structure neonicotinoid insecticide with excellent toxicity against A. gossypii.

Exploration of Quantum Chemistry Methods to Explain Mechanism of Mechanochemical Degradation of Typical Organic Pollutants.

The high-efficiency ball milling treatment technology primarily combines the excitation of oxidation processes with high-speed physical collisions, thereby promoting the reaction processes and enhancing the degradation effectiveness of materials. This technology has gained widespread attention in recent years for its application in the degradation of organic solid chemical pollutants. In this study, quantum chemical density functional theory (DFT) was employed to first analyze the impact of electron addition and subtraction on molecular chemical bonds.

Research Progress in Small-Molecule Detection Using Aptamer-Based SERS Techniques.

Nucleic acid aptamers are single-stranded oligonucleotides that are selected through exponential enrichment (SELEX) technology from synthetic DNA/RNA libraries. These aptamers can specifically recognize and bind to target molecules, serving as specific recognition elements. Surface-enhanced Raman scattering (SERS) spectroscopy is an ultra-sensitive, non-destructive analytical technique that can rapidly acquire the "fingerprint information" of the measured molecules.