Cloning and Preliminary Functional Studies of the Nicotinic Acetylcholine Receptor α6 Subunit in Megalurothrips usitatus (Bagnall).

To investigate the functional role of the nicotinic acetylcholine receptor (nAChR) α6 subunit in Megalurothrips usitatus and its involvement in insecticide resistance, we cloned the nAChR α6 gene (Muα6) using RACE technology and analyzed its spatiotemporal expression patterns via RT-qPCR. Functional studies were further conducted under spinetoram stress. The results showed that Muα6 (GenBank accession: PQ441948) has a full-length sequence of 1569 bp, encoding 523 amino acids with four transmembrane domains (TM1-TM4), six extracellular loops (Loop A-F), and two N-glycosylation sites (Asn100 and Asn144).

Age-Related Differences in Visual Attention to Heritage Tourism: An Eye-Tracking Study.

With the rising significance of visual marketing, differences in how tourists from various age groups visually engage with tourism promotional materials remain insufficiently studied. This study recruited 48 participants and used a quasi-experimental design combined with eye-tracking technology to examine visual attention, scan path patterns, and their relationship to reading performance among different age groups. Independent t-tests, correlation analyses, and Lag Sequential Analysis were conducted to compare the differences between the two groups.

Corrigendum: Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in (thysanoptera: thripidae).

[This corrects the article DOI: 10.3389/fphys.2023.

Association between sleep disorders and myopia in Shanghai adolescents: a cross-sectional survey conducted among junior school students.

Background: Numerous research suggest that longer sleep duration correlates with a reduced likelihood of myopia onset in adolescents. Does total sleep quality influence myopia? This study aims to examine the relationship between sleep disorders in adolescents and their effects on myopia and the risk of acquiring high myopia.

Methods: Data from second-grade middle school students across 16 districts in Shanghai were studied.

Burden of nasopharyngeal carcinoma in Asia from 1990 to 2021.

Objectives: The study aimed to investigate the disease burden of nasopharyngeal carcinoma (NPC) in Asia.

Material And Methods: The Global Burden of Diseases, Injuries, and Risk Factors Study (GBD) 2021 data evaluated NPC metrics by sex, and age in Asia. Estimated annual percentage changes (EAPCs) were calculated to assess the trends of NPC burden.

Hericinosides A-M, Cyathane Diterpene Glycosides with α-Glucosidase Inhibitory Activity from the Medicinal Fungus .

Thirteen new cyathane diterpene glycosides, hericinosides A-M (-), and six known analogs, were isolated from the rice media fermentation of the medicinal fungus . The sugar parts of and were highly modified, forming a unique 5/6/7/5/5/5 ring new skeleton system. All structures were elucidated based on spectroscopic data, and their relative configurations were determined according to the ROESY analysis.

Analysis the Burden of Breast Cancer Among Adolescents and Young Adults Using the Global Burden of Disease 2021.

Background: Unique features and worse outcomes have been reported for breast cancers among adolescents and young adults (AYAs; age 15-39 years). However, there are few up-to-date and comprehensive data to analyze the disease burden of AYA breast cancer.

Patients And Methods: The data of incidence, deaths, and disability-adjusted life years (DALYs) owing to breast cancer was obtained from the Global Burden of Disease (GBD) 2021.

Nine New Glycosylated Compounds from the Leaves of the Medicinal Plant .

Nine new compounds (-), including four dihydrochalcone glycosides, two dibenzofuran glycosides, and two biphenyl glycosides, were isolated from the leaves of the medicinal plant collected in Shennongjia Forestry District (Hubei, China). Their structures were elucidated by comprehensive spectroscopic techniques, including HRESIMS and NMR spectra. All compounds were tested by preliminary biological evaluation for their α-glucosidase inhibitory and NO production activities.

Accelerating T relaxation time measurements of noble gas using transverse low-frequency square-wave magnetic field modulation.

The longitudinal relaxation time (T) of noble gas nuclear spins is a critical parameter for evaluating the performance of an atomic comagnetometer, significantly influencing the signal-to-noise ratio of the system. Traditional measurement techniques, such as the free induction decay method combined with the spin growth technique (FIDSG), are time-consuming for gases with extended T durations, such as Ne, and are prone to substantial environmental variability. Here, we propose the transverse low-frequency square-wave magnetic field modulation (LSMM) method for the rapid measurement of T.

Transfer dynamics of antimicrobial resistance among gram-negative bacteria.

Antimicrobial resistance (AMR) in gram-negative bacteria (GNBs) is a significant global health concern, exacerbated by mobile genetic elements (MGEs). This review examines the transfer of antibiotic resistance genes (ARGs) within and between different species of GNB facilitated by MGEs, focusing on the roles of plasmids and phages. The impact of non-antibiotic chemicals, environmental factors affecting ARG transfer frequency, and underlying molecular mechanisms of bacterial resistance evolution are also discussed.

Malignant phyllodes tumor of the breast with predominant osteosarcoma and chondrosarcomatous differentiation: a rare case report and review of literature.

Background: Phyllodes tumors (PTs), which account for less than 1% of mammary gland tumors, composed of both epithelial and stromal components. If a malignant heterologous component is encountered, PT is considered malignant. Malignant phyllodes tumors (MPTs) only account for 8% to 20% of PTs.

Phellintremulins A-C, antinociceptive sesquiterpenoids from the medicinal fungus Phellinus tremulae.

Phellintremulin A (1), a rearranged sesquiterpenoid with an unprecedented bicyclic backbone, and two previously unreported illudane-type sesquiterpenoids, namely phellintremulin B (2) and phellintremulin C (3), together with two known analogues (±)‒4 and (±)‒5, were isolated from cultures of the medicinal fungus Phellinus tremulae. Their structures and absolute configurations were established by means of spectroscopic data and HRESIMS analyses, as well as ECD and NMR calculations. A plausible biogenesis for 1 was discussed.

A new high-resolution global topographic factor dataset calculated based on SRTM.

Topography is an important factor affecting soil erosion and is measured as a combination of the slope length and slope steepness (LS-factor) in erosion models, like the Chinese Soil Loss Equation. However, global high-resolution LS-factor datasets have rarely been published. Challenges arise when attempting to extract the LS-factor on a global scale.

A Biomimetic Se-nHA/PC Composite Microsphere with Synergistic Immunomodulatory and Osteogenic Ability to Activate Bone Regeneration in Periodontitis.

Accumulation of reactive oxygen species (ROS) in periodontitis exacerbates the destruction of alveolar bone. Therefore, scavenging ROS to reshape the periodontal microenvironment, alleviate the inflammatory response and promote endogenous stem cell osteogenic differentiation may be an effective strategy for treating bone resorption in periodontitis. In this study, sericin-hydroxyapatite nanoparticles (Se-nHA NPs) are synthesized using a biomimetic mineralization method.

Functional diversity of voltage-gated sodium channel in Drosophila suzukii (Matsumura).

Background: The larvae of Drosophila suzukii Matsumura feed directly inside the fruit, causing catastrophic damage to orchards. The misuse of pyrethroid insecticides during the control period has led to increasing resistance of D. suzukii to pyrethroids acting on the voltage-gated sodium channel (VGSC).

ATAC-seq reveals the roles of chromatin accessibility in the chondrocytes of Kashin-Beck disease compared with primary osteoarthritis.

This study aimed to investigate the roles of accessible chromatin in understanding the different pathogeneses between Kashin-Beck disease (KBD) and primary osteoarthritis (OA). Articular cartilages of KBD and OA patients were collected, and after tissue digestion, primary chondrocytes were cultured . Assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) was performed to compare the accessible chromatin differences of chondrocytes between KBD and OA groups.

Correction: Analysis of -glycosylation protein of Kashin-Beck disease chondrocytes derived from induced pluripotent stem cells based on label-free strategies with LC-MS/MS.

Correction for 'Analysis of -glycosylation protein of Kashin-Beck disease chondrocytes derived from induced pluripotent stem cells based on label-free strategies with LC-MS/MS' by Sen Wang , , 2023, https://doi.org/10.1039/d3mo00018d.

Analysis of -glycosylation protein of Kashin-Beck disease chondrocytes derived from induced pluripotent stem cells based on label-free strategies with LC-MS/MS.

We aimed to compare -glycosylation proteins in Kashin-Beck disease (KBD) chondrocytes and normal chondrocytes derived from induced pluripotent stem cells (iPSCs). KBD and normal iPSCs were reprogrammed from human KBD and normal dermal fibroblasts, respectively. Subsequently, chondrocytes were differentiated from KBD and normal iPSCs separately.

Double sodium channel mutation, I265T/L1014F, is possibly related to pyrethroid-resistant in Thrips palmi.

Thrips palmi Karny (Thysanoptera: Thripidae) can harm a variety of agricultural crops and transmit plant viruses, causing heavy economic losses. In the Hainan province of China, pyrethroids were sprayed widely to control T. palmi, which leaded to resistance to pyrethroids in T.

Identification and validation of reference genes for normalization of gene expression analysis using qRT-PCR in (thysanoptera: thripidae).

Gene expression analysis by reverse transcription quantitative polymerase chain reaction (qRT-PCR) has been widely used in research including insects. The selection of appropriate reference genes is the key to obtaining accurate and reliable results from qRT-PCR. However, studies on the expression stability of reference genes in remain lacking.

Phenylpropanoid amides from and their phytotoxic activities against .

Introduction: , an annual malignant weed, has seriously damaged the ecological environment and biodiversity of invasion area. This alien plant gains a competitive advantage by producing some new phytotoxic substances to inhibit the growth of native plants, thus achieving successful invasion. However, the chemical structures, inhibitory functions and action mechanisms of phytotoxic substances of remain unclear.