The lncRNA Jpx participates in testosterone-induced H9c2 cell hypertrophy by targeting the miR-145-5p/Nfatc3 axis.

Cardiac hypertrophy is an adaptive cardiac response to overload. The ensuing decompensation eventually leads to heart failure or sudden death. Testosterone can induce cardiomyocyte hypertrophy, although the underlying mechanism has not been completely elucidated.

Mechanism of parent-of-origin effects revealed by multi-omic data in euro-chinese hybrid pigs.

Parent-of-origin effects refer to the phenomenon whereby the gene expression and corresponding phenotype are influenced by paternal or maternal origin, and uncovering the underlying regulatory mechanisms remains a challenging task. To address this, we designed three sets of trio families by crossing divergent pig breeds to generate sufficient heterozygous loci and collected back fat and longissimus dorsi for multi-omics sequencing. Parental phases of sequencing reads are efficiently determined by leveraging long-read sequencing technology.

TMEFF2 promotes hyperplastic prostate progression by degrading BAX via TRIM17.

Background: Benign prostatic hyperplasia (BPH) is a prevalent pathological condition and a significant, though not exclusive, contributor to lower urinary tract symptoms (LUTS) in aging males. Despite decades of intensive research, the molecular mechanisms underlying BPH remain elusive. We investigated the potential functional roles of TMEFF2 and its underlying mechanisms in prostatic hyperplasia.

The effects of formula milk on development, nutrient absorption, and immune characteristics of piglets.

Background: Breast milk is widely regarded as the optimal nourishment for infants. However, due to physiological or psychosocial factors, many infants do not receive adequate breastfeeding. So, the formula milk is proposed as a substitute of breast milk, and an increasing number of infants are being formula-fed.

Renal epidermoid cyst mimicking renal tuberculous abscess: a case report.

Renal epidermoid cysts (RECs) are exceedingly rare benign cystic lesions, with only 15 histologically confirmed cases reported worldwide to date. Due to their non-specific clinical and radiological features, they are often misdiagnosed preoperatively as infectious or neoplastic conditions. Here, we report a 25-year-old man in whom a complex renal cyst was incidentally identified during a routine health examination.

Hydrogel-Based Sequential Photodynamic Therapy Promotes Wound Healing by Targeting Wound Infection and Inflammation.

Impaired wound healing remains a significant clinical challenge, often exacerbated by bacterial infections and persistent inflammation. Photodynamic therapy (PDT) has demonstrated efficacy in combating bacterial infections; however, its utility in wound healing is limited due to its inability to address inflammation. To overcome this limitation, we have developed a PDT-based sequential therapy.

Obesity induced transcriptional changes in skeletal muscle across different species.

Pigs on high-fat diets maintaining metabolic homeostasis and are resistant to hepatic steatosis, differing from humans and mice. Obesity-induced metabolic dysregulation and inflammation in skeletal muscle are well-studied in humans and mice, but less is known about pig skeletal muscle responses. This study constructs the skeletal muscle transcriptome of obese pigs and integrates it with publicly available transcriptional profiles from obese humans and mice, and ATAC-seq data from lean individuals across species.

Transcriptional Diversity in Response to Aging Across Skeletal Muscles.

Aging leads to a gradual decline in muscle function, yet the mechanisms by which different skeletal muscles respond to aging remain unclear. Here, we constructed transcriptional maps of 11 skeletal muscles with extensive transcriptional diversity from young and old mice. Age-related changes in gene expression displayed distinct tissue-specific patterns, involving muscle diseases and metabolic processes.

Spinel-Layered Heterostructure Enables Reversible Oxygen Redox in Lithium Manganese Oxide.

Lithium-rich manganese-based layered oxides (LRMOs) have emerged as promising cathode materials for next-generation lithium-ion batteries (LIBs), primarily due to their exceptional capacity originating from oxygen redox chemistry. Although LiMnO (LMO) has been conventionally identified as the oxygen redox-active component in LRMOs, this layered material shows neither bulk redox activity nor reversible anion redox behavior in the absence of other transition metals (e.g.

Maternal obesity impairs fetal brown adipogenesis by attenuating fetal FGF21 signaling.

In mammals, maternal obesity typically impairs brown adipose tissue (BAT) formation in fetuses, increasing their risk of metabolic disorders in adulthood. However, the mechanisms behind this phenomenon are not well understood. Our single-nucleus transcriptomic analysis revealed dynamic changes in cell heterogeneity within the fetal interscapular BAT (iBAT) from obese dams, leading to compromised thermogenesis in their offspring.

Gut microbiota orchestrates skeletal muscle development and metabolism in germ-free and SPF pigs.

The gut microbiota, as a crucial symbiotic microbial community in the host, participates in regulating the host's metabolism, immunity, and tissue development. Skeletal muscle is a key tissue for movement and energy metabolism in the body, with its development and function regulated by multiple factors; however, the molecular mechanisms by which the gut microbiota influences skeletal muscle remain unclear. This study utilized germ-free (GF) and specific pathogen-free (SPF) pig models, combined with multiple analytical approaches, to systematically investigate the effects of gut microbiota absence on skeletal muscle development, muscle fiber typing, and metabolism.

Parental Phasing Study Identified Lineage-Specific Variants Associated with Gene Expression and Epigenetic Modifications in European-Chinese Hybrid Pigs.

Understanding how hybrids integrate lineage-specific regulatory variants at the haplotype level is crucial for elucidating the genetic basis of heterosis in livestock. In this study, we established three crossbred pig families derived from distant genetic lineages and systematically identified variants from different lineages, including single nucleotide polymorphisms (SNPs) and structural variations (SVs). At the phase level, we quantitatively analyzed gene expression, four histone modifications (H3K4me3, H3K27ac, H3K4me1, and H3K27me3), and the binding strength of transcription factor (CTCF) in backfat (BF) and longissimus dorsi (LD) muscle.

Low MXene Loading of Epoxy Composite with Enhanced Hydrothermal Resistance.

This work focuses on the hydrothermal aging of two-dimensional layered TiCT (MXene)/epoxy (EP) nanocomposites. MXene/EP composites were successfully prepared by homogeneously dispersing multilayer MXene (m-MXene) and few-layer MXene (f-MXene) into the curing agent, methyl nadic anhydride (MNA). Considering the application, the MXene loading was designed to be 0.

Integrating large-scale meta-GWAS and PigGTEx resources to decipher the genetic basis of 232 complex traits in pigs.

Understanding the molecular and cellular mechanisms underlying complex traits in pigs is crucial for enhancing genetic gain via artificial selection and utilizing pigs as models for human disease and biology. Here, we conducted comprehensive genome-wide association studies (GWAS) followed by a cross-breed meta-analysis for 232 complex traits and a within-breed meta-analysis for 12 traits, using 28.3 million imputed sequence variants in 70 328 animals across 14 pig breeds.

Single cell transcriptome profiling of immune tissues from germ-free and specific pathogen-free piglet.

The commensal microbiota provides immunomodulatory signals during the development, differentiation and activation of immune cells, and is crucial for maintaining host immune homeostasis. However, the systematic effects of commensal microbiota on host immunity based on large animal model at the single-cell level remain to be resolved. Here, we utilized single-cell RNA sequencing (scRNA-seq) to analyze the transcriptome profiling containing 57,720 cells from three important immune tissues [Peyer's patches (PP), mesenteric lymph node (MLN), and spleen] of germ-free (GF) and specific pathogen-free (SPF) piglet.

Two telomere-to-telomere pig genome assemblies and pan-genome analyses provide insights into genomic structural landscape and genetic adaptations.

This study presented two high-precision telomere-to-telomere genome assemblies for Min and Rongchang pigs, including a detailed exploration of the telomeric and centromeric regions. By integrating pan-genome and multi-omics analyses, structural variations linked to genetic adaptation were identified, providing a valuable resource for advancing pig breeding and genetic improvement.

Insights into high-altitude adaptation and meat quality regulation by gastrointestinal metabolites in Tibetan and black pigs.

Introduction: Tibetan pigs, native to the Qinghai-Tibet Plateau, have adapted over millennia to extreme conditions such as low oxygen, harsh cold, and high UV radiation, impacting their muscle characteristics and digestive tract microbiota. The quality of pork from Tibetan pigs (TP) and black pigs (BP) is influenced by various factors, including genetics, diet, and environmental adaptation. However, the specific influence of digestive tract microbiota metabolites on muscle traits remains poorly understood.

The Farm Animal Genotype-Tissue Expression (FarmGTEx) Project.

Genetic mutation and drift, coupled with natural and human-mediated selection and migration, have produced a wide variety of genotypes and phenotypes in farmed animals. We here introduce the Farm Animal Genotype-Tissue Expression (FarmGTEx) Project, which aims to elucidate the genetic determinants of gene expression across 16 terrestrial and aquatic domestic species under diverse biological and environmental contexts. For each species, we aim to collect multiomics data, particularly genomics and transcriptomics, from 50 tissues of 1,000 healthy adults and 200 additional animals representing a specific context.

Gut Metagenome Reveals the Microbiome Signatures in Tibetan and Black Pigs.

The harsh conditions of the Qinghai-Tibet Plateau pose significant physiological challenges to local fauna, often resulting in gastrointestinal disorders. However, Tibetan pigs have exhibited remarkable adaptability to the high-altitude stress of the Tibetan Plateau, a phenomenon that remains not fully understood in terms of their gastrointestinal microbiota. This study collected 57 gastrointestinal tract samples from Tibetan pigs ( = 6) and plain black pigs ( = 6) with comparable genetic backgrounds.

A dataset of single-cell transcriptomic atlas of Bama pig and potential marker genes across seven tissues.

The use of single-cell sequencing technology for single-cell transcriptomics studies in pigs is expanding progressively. However, the comprehensive classification of cell types across different anatomical tissues and organs of pig in multiple datasets remains relatively limited. This study employs single-cell and single-nucleus sequencing technologies in Bama pig to identify unique marker genes and their corresponding transcriptomic profiles across diverse cell types in various anatomical tissues and organs, including subcutaneous fat, visceral fat, psoas major muscle, liver, spleen, lung, and kidney.

A glycopeptide-based pH-responsive hydrogel promotes diabetic wound healing via antimicrobial and remodeling microenvironment.

Treating bacterium-infected diabetic wounds remains a major medical challenge. Antimicrobial activity, remodeling of oxidative stress-heavy and angiogenesis-impaired microenvironments are critical factors for effective wound healing. Hydrogels can function as drug delivery systems that encompass all these capabilities to enhance wound healing.

Identification of SNPs in the second intron of and their Association with growth traits in Nanjiang Yellow goat.

Insulin-like Growth Factor 2 mRNA-binding Protein 1 () is a candidate gene of significant interest for modulating economically important traits in livestock and poultry. The second intron of has been implicated in growth-related traits, though its precise mechanistic role remains elusive. Initial resequencing analyses in our laboratory indicated strong selective pressures on the genomic region, prompting the selection and identification of several single nucleotide polymorphisms (SNPs).

Identification and Functional Analysis of Key microRNAs in the Early Extrauterine Environmental Adaptation of Piglets.

Neonatal mammals must rapidly adapt to significant physiological changes during the transition from the intrauterine to extrauterine environments. This adaptation, particularly in the metabolic and respiratory systems, is essential for survival. MicroRNAs (miRNAs) are small noncoding RNAs that regulate various physiological and pathological processes by binding to the 3' untranslated regions of mRNAs.

AnimalGWASAtlas: Annotation and prioritization of GWAS loci and quantitative trait loci for animal complex traits.

Genome-wide association study (GWAS) and quantitative trait locus (QTL) mapping methods provide valuable insights and opportunities for identifying functional gene underlying phenotype formation. However, the majority of GWAS risk loci and QTLs located in noncoding regions poses significant challenges in pinpointing the protein-coding genes associated with specific traits. Moreover, growing evidence suggests not all GWAS risk loci and QTLs are functional, emphasizing the critical need for prioritizing causal sites-a task of paramount importance for biologists.