Bromide-Catalyzed Radical Cyclization of Biarylboronic Acids and Se/Te Powder.

Herein, a metal-free and versatile strategy was successfully reported for the divergent synthesis of various functionalized dibenzo[,]selenophenes, dibenzo[,]tellurophenes, and phenoxaselenines in moderate to excellent yields in one-pot. This method utilized commercially available, cheap, and sustainable ammonium bromide as the catalyst to promote the radical cyclization of biarylboronic acids with elemental Se/Te. Comparatively, this method was highlighted by its readily available starting material, broad substrate scope, simple catalytic system, easy operation, good safety, being strongly oxidant-free, and easily scalable.

Targeting RPS6KC1 to overcome enzalutamide resistance in prostate cancer.

The androgen receptor signaling inhibitor enzalutamide (Enz) is one the primary therapeutic drugs for advanced prostate cancer (PCa). Nevertheless, most of patients ultimately develop resistance to Enz. Through an integrated analysis of CRISPR genome-wide and kinome-wide screens, coupled with observations of elevated expression levels in Enz-resistant cell lines and PCa tumor tissues, our study identified RPS6KC1 as a novel essential gene implicated in Enz resistance.

Quantification and discovery of quality control chemical markers for Shouhui Tongbian capsules by UPLC-MS/MS.

Guided by the principles of Analytical Quality by Design (AQbD), a highly selective and sensitive LC-MS/MS method was established for the simultaneous quantification of 15 compounds in Shouhui Tongbian capsules, enabling their effective quality control. The separation was carried out on a BEH C18 column (1.7 μm, 2.

Synthesis of 4-(arylthio)isoquinolin-1(2)-ones TBHP-mediated sequential amidation/thioetherification of -alkylisoquinolinium salts.

Here, a simple, efficient, and practical method for the assembly of 4-(arylthio)isoquinolin-1(2)-ones TBHP-mediated 1,4-difunctionalization of -alkylisoquinolinium iodide salts under metal-free conditions is reported. This strategy features readily available starting materials, operational simplicity, and metal-free conditions. Remarkably, a variety of (hetero)aryl/alkyl thiols, diphenyl disulfide, diphenyl diselenide, and -alkylisoquinolinium bromides or chlorides are compatible with this method, which generates the desired 4-(arylthio)isoquinolin-1(2)-ones in 30-92% yields.

The Application of Quantitative H-NMR for the Determination of Melatonin and Vitamin B6 in Commercial Melatonin Products.

Melatonin supplements have been widely used to improve sleep quality and overcome sleep disorders, with melatonin and vitamin B6 serving as the primary active ingredients. This study developed a novel analytical method for the simultaneous quantification of melatonin and vitamin B6 using H-NMR spectroscopy. The characteristic signals of melatonin and vitamin B6 hydrochloride at δ 7.

Extraction of Rubidium and Cesium from a Variety of Resources: A Review.

In recent years, with the development of science and technology and the transformation of economic structures, rubidium and cesium have gradually become indispensable rare metal resources as important materials for high-tech industries. However, the relationship between supply and demand of resources is unbalanced, industrial demand is much higher than production, and the rubidium and cesium resources in hard rock minerals such as traditional pegmatite minerals are no longer enough to support global scientific and technological upgrading. There is therefore an urgent need to expand sources of resource extraction and recovery to meet market demand.

Electrochemical Synthesis of 2-Selenylnaphthalenes with Alkynyl Alkyl Alcohols and Diselenides.

Herein, the facile synthesis of 2-selenylnaphthalene derivatives under mild conditions is accessible via electrocatalytic sequential selenylation/cyclization/aromatization of readily available starting material, which is successfully demonstrated. The potential synthetic utility of this method is demonstrated by its wide substrate scope, excellent functional group compatibility, high efficiency, metal-free, chemical-oxidant-free, easy operation, and easy scalability. The detailed mechanistic experiments indicate that this strategy possibly proceeds via a radical pathway.

Mechanotransductive Activation of PPAR-γ by Low-Intensity Pulsed Ultrasound Induces Contractile Phenotype in Corpus Spongiosum Smooth Muscle Cells.

Previously, we found that the pathological changes in the corpus spongiosum (CS) in hypospadias were mainly localized within smooth muscle tissue, presenting as a transformation from the contraction phenotype to synthesis. The role of low-intensity pulsed ultrasound (LIPUS) in regulating smooth muscle cells (SMCs) and angiogenesis has been confirmed. To demonstrate the feasibility of regulating the phenotypic transformation of corpus spongiosum smooth muscle cells (CSSMCs) in hypospadias using LIPUS and to explore the potential mechanisms.

-Phosphinoylaminophthalimide: a phosphinoyl radical precursor applied in phosphinoyl-substituted phenanthridine and ketone synthesis.

A new phosphinoyl radical precursor, -phosphinoylaminophthalimide (PAPI), was developed. The precursor exhibits several practical advantages: facile synthesis from commercially available starting materials, cost-effectiveness at scale, and excellent air and moisture stability under ambient storage conditions. Phosphinoyl radical was generated under basic and oxidative conditions, and captured by isonitrile and olefin, yielding phosphinoyl-substituted phenanthridines and α-phosphinoyl ketones, respectively.

Synthesis of Furan-Tethered Benzocyclobutenes via Protonic Acid-Catalyzed 1,4-Sulfinate Migration-Annulation.

A protonic acid-catalyzed cascade of conjugated enynones was realized for synthesizing multisubstituted furans containing benzocyclobutene with high yields and simple procedures. Employing inexpensive sulfanilic acid as a catalyst, this method features a key 1,4-sulfinate migration and avoids the use of transition metal catalysts. Substrates that are unreactive under metal-mediated catalysis are applicable due to proton transfer processes.

Development of an LC-MS/MS method for astaxanthin quantification in shrimp tissues and its application to detect astaxanthin variations during ovary development stages.

Objective: In this study, a highly selective and sensitive LC-MS/MS method was developed to comprehensively analyze the distribution of astaxanthin across various tissues of , as well as its variations in the hepatopancreas and ovary throughout ovarian development.

Methods: The separation was performed on a BEH C8 column (1.7 μm, 2.

KRT5 TP63-expressing urothelial basal cells act as a driver to bladder urothelium regeneration in rabbit.

Background: Urothelial regeneration is a crucial part of bladder tissue engineering. However, there is a lack of ideal "seed cells" in current practices. Here, we demonstrated that a sub-population of p63 positive basal cells could be activated and differentiate into intermediate and superficial umbrella cells after full-thickness mucosal resection in rabbit.

The Innovation Paradox in Emerging Pharmaceutical Markets: Barriers and Opportunities for Sustainable Development.

Emerging pharmaceutical markets like Brazil, India, and China have seen significant growth due to rising medication demand, expanding middle-class access, and government support. However, this growth often focuses on cost-driven strategies like generic drug production rather than innovation. Challenges such as fragmented regulatory systems, limited infrastructure, low R&D budgets, and dependence on imported active pharmaceutical ingredients (APIs) limit global competitiveness in drug innovation.

Phylogenetic relationship, genomic heterogeneity, and population structure of Yersinia enterocolitica biotype 1A isolated from pork and poultry meat.

Yersinia enterocolitica is a zoonotic pathogen commonly found in livestock and poultry and their meat products. It causes enteric yersiniosis in humans, primarily through contaminated food consumption. In this study, 352 retail meat samples, including raw and cooked pork and poultry, were collected from Weifang City, Shandong Province, China.

Synthesis of 1,3,4-thiadiazoles from elemental sulfur three-component cyclization under metal-free conditions.

In this work, we demonstrate the synthesis of a variety of 2,5-disubstituted 1,3,4-thiadiazoles in 28-85% yields three-component cyclization of 2-methylquinolines, arylhydrazides, and S powder under metal-free conditions. This approach stands out due to its broad substrate scope, excellent functional group tolerance, additive-free nature, base-free nature, easy operation, and simple reaction system. This work offers a simple, concise, and convenient pathway for the assembly of a variety of 1,3,4-thiadiazoles, which is complementary to previous methods.

Small Animal Positron Emission Tomography Imaging of a Triple-Negative Breast Cancer Model Using the Ga-Labeled pH (Low) Insertion Peptide-Like Peptide YJL-11.

To prepare a novel Ga-labeled pH (low) insertion peptide-like peptide, YJL-11, and study its ability to be used as a probe for the diagnosis of triple-negative breast cancer (TNBC) via imaging of tumor-bearing nude mice. Circular dichroism (CD) analysis of YJL-11 was performed to assess its secondary structure. YJL-11 was labeled with Ga, and the biodistribution of Ga-YJL-11 in MDA-MB-231 xenograft mice was evaluated.

Development of a Near-Infrared Probe for Enhancing Cancer Therapy by Mitigating Pyroptosis-Induced Inflammation.

Pyroptosis, a specialized cell death mechanism crucial in cancer therapy, is distinct from apoptosis and primary necrosis. It is linked to inflammation and reactive oxygen species, particularly peroxynitrite (ONOO), which is implicated in disease. Monitoring pyroptosis is challenging due to its connection with cellular polarity and the tumor environment.

The impact of aztreonam-clavulanic acid exposure on gene expression and mutant selection using a multidrug-resistant .

Multidrug-resistant poses a significant threat to the healthcare system by causing treatment failure in infected patients. The use of a beta-lactam in combination with a beta-lactamase inhibitor has been shown to be an effective strategy to solve this problem. antimicrobial susceptibility experiments have demonstrated the antimicrobial activity of aztreonam and clavulanate.

Health locus of control and associated factors among community-dwelling older adults: a cross-sectional descriptive study.

Background: To know the level of health locus of control (HLC) and investigate the factors associated with HLC among community-dwelling older adults.

Method: A total of 470 older adults completed the Multidimensional Health Locus of Control Scale, the Activities of Daily Living Scale, the Geriatric Depression Scale, and the Lubben Social Network Scale. Hierarchical multiple regression was used to explore the factors associated with HLC.

Simultaneous Quantification of Total Antibody and Conjugated Payload for DS001 in Rat Serum Using a Hybrid Immuno-Capture LC-MS/MS.

Antibody-drug conjugates (ADCs) are intricate compounds that pose significant challenges in bioanalytical characterization. Therefore, multiple bioanalytical methods are required to comprehensively elucidate their pharmacokinetic (PK) profiles. In this study, we investigated DS001, an ADC consisting of a humanized monoclonal antibody (hRS7), a cleavable chemical linker, and the microtubule inhibitor monomethyl auristatin E (MMAE), with a drug-to-antibody ratio (DAR) of 8.

Draft Whole-Genome Sequencing and Phenotypic Analysis of from Retail Aquatic Products in Weifang.

is one of the most common causative agents of infectious diarrhea in humans, but in China, there are very limited data on the presence of in aquatic products. This study describes the isolation of from aquatic products in Weifang, China, from April 2022 to April 2023. Seven out of 160 (4.

Study on the nutritional relationships in mycelia and fruiting bodies of Hypsizygus marmoreus under defined nutrient conditions.

Edible fungal fruiting bodies develop from mycelia that experienced physiological knotting, establishing a tight nutrient connection between mycelia and fruiting bodies. Despite this connection, limited research has explored this field. This study aimed to elucidate the crude polysaccharide and protein contents in mycelia and fruiting bodies of 5 main edible fungi cultivars: Flammulina velutipes, Hypsizygus marmoreus, Lyophyllum decastes etc.

Spotlight on Mitochondrial Health: A Trailblazing Fluorescent Tool for Cancer Detection and Surgical Guidance.

Mitochondria play a pivotal role in maintaining normal physiological functions. Mitochondrial autophagy, namely, mitophagy, is a selective catabolic disposal of impaired mitochondria through an autophagic mechanism during episodes of mitochondrial harm. This selective removal, e.

Spatiotemporal wind speed forecasting using conditional local convolution and multidimensional meteorology features.

Wind speed prediction is crucial for precisely wind power forecasting and reduced maintenance costs. Highland regions, which possess a considerable wind potential, present complex meteorological conditions, making wind speed prediction challenging. Traditional weather forecasting relies on complex statistical methods and extensive prior knowledge.

Dual-Functional Fluorescent Probe in the Diagnosis of Liver Injury and the Evaluation of Drug Therapy with Double Signal Amplification.

Viscosity and polarity are crucial microenvironmental parameters within cells, intimately linked to the physiological activities of organisms. We constructed and synthesized an innovative dual-functional fluorescent probe, DHBP. In the green channel, the fluorescence signal notably intensifies with decreasing environmental polarity, while in the red channel, fluorescence signal amplification occurs due to the collaborative effects of viscosity and polarity, resulting in more pronounced changes.