Super-Resolution Imaging Captures Mitophagy Dynamics: Tricolor Fluorescence Labels Mitochondria, Autophagosomes, and Lysosomes.

Mitophagy is a vital lysosome-dependent process in which damaged mitochondria exhibiting elevated HO production are selectively engulfed by autophagosomes and delivered to lysosomes for degradation, thereby maintaining intracellular homeostasis. Consequently, monitoring mitophagy holds significant potential for disease diagnosis and therapeutic development. In this study, HO-activated lysosome-targeted fluorescent probe, , was developed for the super-resolution imaging of the mitophagic process.

Enhanced photocatalytic carbon dioxide reduction over iron tungstate/covalent organic framework heterojunctions by the induced unified adsorption and reduction sites.

Inorganic-organic S-scheme heterostructure photocatalysts have demonstrated exceptional potential in boosting charge separation for CO reduction. However, the reduction typically occurs at the inorganic site, resulting in underutilization of high CO adsorption capacity of organic material. To resolve this mismatch, we combined a low conduction band iron tungstate (FeWO) with a dioxin-linked covalent organic framework (COF) to construct S-scheme heterojunction, where the COF serve as the reduction photocatalysts and FeWO act as the oxidation photocatalysts, for photocatalytic CO reduction.

Multisignal Proportional Optical Chiral Sensing: Photoresponsive Molecular Probe with High Enantioselectivity for Stereospecific Molecular Recognition.

The differences in the spatial structures of chiral enantiomers often lead to variations in physiological activity. Focusing on the stereoselective behavior of chiral enantiomers, timely and accurate risk assessment has become an important issue in pharmaceutical research development. The development of high-selectivity and rapid discrimination detection methods is the key to chiral enantiomer-related research.

Fabrication of Zwitterionized Nanocellulose/Polyvinyl Alcohol Composite Hydrogels Derived from Camellia Oleifera Shells for High-Performance Flexible Sensing.

To address the growing demand for environmentally friendly flexible sensors, here, a composite hydrogel of nanocellulose (NC) and polyvinyl alcohol (PVA) was designed and fabricated using shells as a sustainable alternative to petroleum-based raw materials. Firstly, NC was extracted from shells and modified with 2-chloropropyl chloride to obtain a nanocellulose-based initiator (Init-NC) for atomic transfer radical polymerization (ATRP). Subsequently, sulfonyl betaine methacrylate (SBMA) was polymerized by Init-NC initiating to yield zwitterion-functionalized nanocellulose (NC-PSBMA).

Engineering intermolecular C-F···C=O interactions in covalent organic framework promotes dual-path HO photosynthesis for sustainable disinfection.

The urgent need to address the high prevalence of waterborne diseases in underdeveloped regions necessitates the development of economically viable, decentralized, and sunlight-assisted disinfection techniques. An encouraging solution lies in the utilization of photosynthesized HO to initiate advanced oxidation processes (AOPs). However, challenges persist in the quest to develop efficient photocatalysts and reactor designs.

Development of a Near-Infrared Probe for Enhancing Cancer Therapy by Mitigating Pyroptosis-Induced Inflammation.

Pyroptosis, a specialized cell death mechanism crucial in cancer therapy, is distinct from apoptosis and primary necrosis. It is linked to inflammation and reactive oxygen species, particularly peroxynitrite (ONOO), which is implicated in disease. Monitoring pyroptosis is challenging due to its connection with cellular polarity and the tumor environment.

Super-Resolution Mitochondrial Fluorescent Probe for Accurate Monitoring of Drug-Induced Liver Injury.

Drug-induced liver injury (DILI) has emerged as an urgent clinical challenge. It is characterized by mitochondrial dysfunction in liver cells, which leads to abnormal changes in HO levels within the mitochondria. Super-resolution imaging allows for the observation of the fine structure of mitochondria at the nanometer scale, potentially enabling the detection of mitochondrial HO levels during DILI at the subcellular organelle level.

Ratiometric Fluorescent Probe for Super-Resolution Imaging of Lysosome HClO in Ferroptosis Cells.

Ferroptosis is an iron-dependent programmed cell death that is characterized by the dysregulation of lipid reactive oxygen species (ROS) production, causing abnormal changes in hypochlorous acid (HClO) levels in lysosomes. Super-resolution imaging can observe the fine structure of the lysosome at the nanometer level; therefore, it can be used to detect lysosome HClO levels during ferroptosis at the suborganelle level. Herein, we utilize a ratiometric fluorescent probe, , for super-resolution imaging of lysosome HClO.

Analysis of Molar Substitution of Hydroxybutyl Group by Zeisel Reaction in Starch Ethers.

A new etherified starch, δ-hydroxybutyl starch (δ-HBS), was prepared by utilising 4-chlorobutan-1-ol as the etherifying reagent. The method of Zeisel gas chromatography for the determination of the molar substitution was described. This technique offers a simple and rapid method for quantitative analysis with reproducible results.

FAM3A enhances adipogenesis of 3T3-L1 preadipocytes via activation of ATP-P2 receptor-Akt signaling pathway.

FAM3A plays important roles in regulating hepatic glucose/lipid metabolism and the proliferation of VSMCs. This study determined the role and mechanism of FAM3A in the adipogenesis of 3T3-L1 preadipocytes. During the adipogenesis of 3T3-L1 preadipocytes, FAM3A expression was significantly increased.