Programmed cell death-driven remodeling of the melanoma microenvironment enables prognostic stratification and therapeutic prediction.

Background And Objective: Melanoma exhibits profound biological complexity, driven by immune evasion, phenotypic plasticity, and resistance to therapy. While programmed cell death (PCD) shapes tumor-immune interactions, its mechanistic landscape in melanoma remains incompletely defined. This study aims to comprehensively characterize PCD-related signatures and their associations with tumor heterogeneity, prognosis, and immunotherapeutic outcomes.

Adaptive Graph Prompting Meets Contrastive Learning: A Multi-View Framework for Metabolite-Disease Association Prediction.

Metabolite-disease associations (MDAs) are critical for advancing precision medicine, yet existing computational methods face challenges in data sparsity, noise robustness, and feature representation. We propose GPLCL (graph prompt-enhanced contrastive learning), a novel multi-view graph learning framework integrating adaptive graph prompting and contrastive learning. GPLCL introduces enhanced graph prompt features (GPF +) with attention-based node adaptation, enabling dynamic feature recalibration.

FLAIR hyperintense cortical lesions in myelin oligodendrocyte glycoprotein-associated encephalitis with seizures in children: a retrospective single-center case series.

Background And Purpose: In recent years, the number of case reports concerning fluid-attenuated inversion recovery (FLAIR) hyperintense cortical lesions in myelin oligodendrocyte glycoprotein (MOG) -associated encephalitis with seizures (FLAMES) has been gradually increasing. However, within the pediatric demographic, there remains a lack of related serial reports. This study was designed to characterize the clinical features and prognosis of FLAMES in pediatric patients.

A comprehensive characterization of metabolic signatures-hypoxia, glycolysis, and lactylation-in non-healing diabetic foot ulcers.

Background And Objective: Diabetic foot ulcers (DFUs) are chronic complications of diabetes, driven by metabolic dysregulation and impaired wound healing. This study investigates the roles of hypoxia, glycolysis, and lactylation in DFUs and identifies potential diagnostic and therapeutic biomarkers.

Methods: Single-cell RNA sequencing (scRNA-seq) was employed to assess cellular diversity, metabolic states, and intercellular communication in DFUs.

Research progress on the regulation of plant growth and development by TOR kinase.

The target of rapamycin (TOR) is a conserved serine/threonine protein kinase in eukaryotes. Numerous studies in yeast and animals have found that TOR kinase senses the status of intracellular nutrients and hormonal signals and participates in the regulation of diseases such as cancer through processes such as protein translation, gene transcription, material metabolism, and autophagy. The medical community has now identified TOR kinase as a key target for the treatment of the aforementioned diseases, conducting research and clinical trials for related drugs.

Optic Nerve Invasion in Retinoblastoma: Impact of Eye Salvage and Adjuvant Chemotherapy.

Purpose: Optic nerve invasion (ONI) is an important risk factor for extraocular metastasis in retinoblastoma. This study evaluates the impact of pre-enucleation chemotherapy, delayed enucleation, and adjuvant chemotherapy on survival in patients with varying degree of ONI on histopathology.

Methods: A retrospective review of consecutive enucleated eyes with any degree of ONI from 29 Chinese treatment centers 2012-2017.

Progress in research on nutrition, neuroinflammation and dopaminergic alterations in Tic disorders.

Tic disorders (TD) represent a prevalent neurodevelopmental condition in children, characterised by involuntary, sudden motor or vocal tics. Dysfunction of the dopamine system plays a pivotal role in the pathogenesis of TD. Recent findings indicate that deep brain stimulation, by modulating striatal dopamine release, substantially alleviates tic symptoms.

Dihydromyricetin mitigates abdominal aortic aneurysm transcriptional and post-transcriptional regulation of heme oxygenase-1 in vascular smooth muscle cells.

Abdominal aortic aneurysm (AAA) is a deadly condition of the aorta, carrying a significant risk of death upon rupture. Currently, there is a dearth of efficacious pharmaceutical interventions to impede the advancement of AAA and avert it from rupturing. Here, we investigated dihydromyricetin (DHM), one of the predominant bioactive flavonoids in (), as a potential agent for inhibiting AAA.

Serum lipid metabolites predict the efficacy of intravenous immune globulin therapy in patients with pediatric dilated cardiomyopathy.

The response of pediatric dilated cardiomyopathy (PDCM) patients to intravenous immune globulin (IVIG) varies from cardiac functional recovery to heart transplantation and even death. IVIG therapy can significantly improve the left ventricular ejection fraction (LVEF) in some PDCM patients, but indicators for evaluating the response to IVIG therapy are lacking. Lipid metabolic disturbance is associated with changes in cardiac function, but no studies have examined the associations between lipidomics markers and the efficacy of IVIG.

Adipocyte exosome miR-4472 inhibits glucose uptake in skeletal muscle through downregulation of MEF2D.

Aims/introduction: Previous studies have found that miR-4472 is overexpressed in the serum of individuals with obesity and type 2 diabetes mellitus (T2DM), which may participate in the process of obesity-induced T2DM. However, a role for miR-4472 in the process has not been demonstrated. Here, we aim to investigate whether the increased content of miR-4472 in adipose tissue derived from exosomes inhibits glucose uptake in skeletal muscle by downregulating the expression of its target gene.

Exosome-derived miR-548ag drives hepatic lipid accumulation via upregulating FASN through inhibition of DNMT3B.

Metabolic dysfunction-associated steatotic liver disease (MASLD) is the leading cause of chronic liver disease worldwide. This study investigates the role of serum miR-548ag in regulating lipid metabolism and its contribution to MASLD in obesity. We found that miR-548ag levels were significantly elevated in the serum of both obese and MASLD patients and positively correlated with body mass index, fasting plasma glucose, triglycerides, total cholesterol, LDL, HDL, aspartate aminotransferase, and alanine aminotransferase levels.

Targeting mitochondrial DNA-STING-NF-κB Axis-mediated microglia activation by cryptotanshinone alleviates ischemic retinopathy.

Background: Ischemic retinopathy, a leading cause of vision impairment, involves oxidative stress and dysregulated inflammation, with microglia playing a key role. Cryptotanshinone (CTS), a bioactive compound from Salvia miltiorrhiza, exhibits anti-inflammatory and antioxidant properties and thus has the potential for development as a therapeutic agent. However, the actual mechanism of action of CTS in ischemic retinopathy is not known.

Recent advances in biomimetic nanodelivery systems for cancer Immunotherapy.

Tumor immunotherapy is a developing and promising therapeutic method. However, the mechanism of tumor immune microenvironment and individual differences of patients make the clinical application of immunotherapy still very limited. The resulting targeting of the tumor environment and immune system is a suitable strategy for tumor therapy.

Influences of Normative Medical Nutrition Management Based on Health Concepts on Weight Control and Maternal and Infant Outcomes in Women of Advanced Maternal Age.

Background: We aimed to explore the effects of normative medical nutrition management based on health concepts on weight control and maternal and infant outcomes in women of advanced maternal age.

Methods: Overall, 112 women of advanced maternal age were divided into the control group and observation group by random number table method. The control group was given routine intervention, and the observation group was given normative medical nutrition management based on health concepts.

Downregulation of tropomyosin 2 promotes the progression of lung adenocarcinoma by regulating neutrophil infiltration through neutrophil elastase.

Lung adenocarcinoma (LUAD) is a common malignant tumor in the lung that seriously endangers the health of people worldwide. The neutrophil-associated inflammatory microenvironment contributes to the activation of tumor cells. In this study, we report a role of tumor-associated neutrophils (TANs) promote tumor progression of LUAD by crosstalk between neutrophils and tumor cells.

Comparative analysis of left atrial volume and strain parameters in hemodialysis vs. peritoneal dialysis patients using four-dimensional automatic quantification technology.

Background: Cardiovascular complications remain a leading cause of mortality in patients with end-stage kidney disease undergoing dialysis. This study aimed to evaluate differences in left atrial (LA) structure and function between hemodialysis and peritoneal dialysis patients using four-dimensional automatic left atrial quantification (4D Auto LAQ) technology.

Methods: This study included 125 patients with end-stage kidney disease, categorized into hemodialysis ( = 69) and peritoneal dialysis ( = 56) groups.

Colchicine Inhibits Smooth Muscle Cell Phenotypic Switch and Aortic Dissection in Mice-Brief Report.

Background: Aortic dissection (AD) is a severe cardiovascular disorder characterized by intimal tearing and subsequent delamination of the aortic wall. The pathogenesis of AD primarily involves the phenotypic switch of vascular smooth muscle cells (VSMCs), degradation of the extracellular matrix, and chronic vascular inflammation. Colchicine, an alkaloid derived from , is a Food and Drug Administration-approved anti-inflammatory drug with established therapeutic applications in cardiovascular diseases.

Biodegradation of S-Triazine Herbicides Under Saline Conditions by PC, a New Halotolerant Bacterial Isolate: Insights into Both the Degradative Pathway and Mechanisms of Tolerance to High Salt Concentrations.

In this study, a halotolerant bacterial strain was isolated and identified. This bacterium was confirmed to efficiently degrade s-triazine herbicides under saline conditions. The optimal conditions for the metabolism and growth of this strain were determined through single-factor tests.

Congenital long QT syndrome caused by a KCNH2 pathogenic variant exhibiting "motor seizures": a case report and literature review.

A retrospective analysis was conducted to evaluate the clinical characteristics, diagnostic challenges, and management strategies in a child with congenital long QT syndrome (cLQTS) caused by a KCNH2 gene pathogenic variant presenting as "motor seizures". The case involved a 10-year-old boy with a two-year history of recurrent loss of consciousness, which had worsened during the preceding week. Clinical manifestations included sudden episodes of unconsciousness, rightward strabismus of both eyes, cyanosis of the lips, guttural vocalizations, rigidity and shaking of the upper limbs, and urinary incontinence.

MultiEYE: Dataset and Benchmark for OCT-Enhanced Retinal Disease Recognition From Fundus Images.

Existing multi-modal learning methods on fundus and OCT images mostly require both modalities to be available and strictly paired for training and testing, which appears less practical in clinical scenarios. To expand the scope of clinical applications, we formulate a novel setting, "OCT-enhanced disease recognition from fundus images", that allows for the use of unpaired multi-modal data during the training phase, and relies on the widespread fundus photographs for testing. To benchmark this setting, we present the first large multi-modal multi-class dataset for eye disease diagnosis, MultiEYE, and propose an OCT-assisted Conceptual Distillation Approach (OCT-CoDA), which employs semantically rich concepts to extract disease-related knowledge from OCT images and leverages them into the fundus model.

The value of slough in wounds for the diagnosis of microbiology cultivation.

Slough is not currently a recommended microbiology culture specimen. The aim of our work is to analyse the concordance of results between slough and swab samples for cultures. A single-centre, retrospective, cross-sectional study involving patients (n = 131) with hard to heal wounds was conducted from October 2022 to October 2023.

Factors Affecting Medication Adherence in Middle-Aged and Elderly Patients in China: A Cross-Sectional Study.

BACKGROUND As the population ages, comorbidities among elderly patients are becoming a significant problem, due to associated risks and mortality. Medication adherence is crucial for controlling chronic diseases and improving patient outcomes; therefore, it is important to understand medication adherence among hospitalized patients. MATERIAL AND METHODS A cross-sectional study was conducted from June 2023 to April 2024 in 2 tertiary hospitals in Wuhu City, Anhui Province, China.

Functional analysis of the PIP5K1A gene in Liaoning Cashmere goats: an investigation based on bioinformatics, tissue localization, and biological functions.

Liaoning cashmere goat is an outstanding breed in China primarily for cashmere production, with strict controls against genetic outflow. Melatonin(MT) is a key factor affecting cashmere growth, and preliminary transcriptome sequencing indicated that melatonin upregulates the expression of the PIP5K1A gene in skin fibroblasts. To predict the physicochemical properties of PIP5K1A in Liaoning cashmere goats, ascertain the tissue localization of PIP5K1A in their skin, and explore the role and mechanism of PIP5K1A in the proliferation of skin fibroblasts.

Two new phytoecdysteroids isolated from .

Two new phytoecdysteroids, (24)-20,26-dihydroxy-24(28)-dehydro-28-methyl-ecdysone (), (24)-22,25-epoxy-2,3,14,20-tetrahydroxyergosta-7,24(28)-dien-28-methyl-6-one (), together with eleven known compounds (-) were isolated from the whole plant of L. The chemical structures of the compounds were elucidated by 1D and 2D NMR and HR-ESI-MS and compared with data from the literature. The isolated compounds were evaluated for their inhibitory effects on NO, TNF-α and IL-6 production in LPS-induced RAW 264.

The environmental impact of extracellular matrix preparation.

The extracellular matrix (ECM) is a network of proteins and other molecules that encase and support cells and tissues in the body. As clinical and biotechnological uses of ECM are expanding, it is essential to assess the environmental impact associated with its production. Due to high levels of customization, various laboratories employ distinct methods; therefore, this study evaluates three common protocols.