Variations in Center-level Practices Across US Transplant Centers Are Associated With Time to Relisting and Retransplantation in Patients With Graft Failure.

Rationale & Objective: There is known variability in the management of kidney transplant recipients facing graft failure. We hypothesized that variations in the timing of care transitions, immunosuppression weaning, and re-evaluation processes would be associated with differential access to retransplantation and relisting.

Study Design: An observational study.

Molecularly imprinted polymer-based system for simultaneous fluorescence quantification of malachite green and crystal violet.

CdTe QD-doped MIP-based TLC combined with a miniaturized fluorimeter system (namely the system) was developed to simultaneously detect malachite green (4-[(4-(dimethylamino)phenyl)(phenyl)methyl]-N,N-dimethylbenzenaminium chloride, MG) and crystal violet (4-(Bis{4-(dimethylamino)phenyl}methylidene)-N,N-dimethylcyclohexa-2,5-dien-1-iminium chloride, CV). The system integrated CdTe quantum dots-based molecularly imprinted polymers nanoparticles (CdTe-MIP/SiO NPs) with thin layer chromatography (TLC) plates. Firstly, CdTe-MIP/SiO NPs were synthesized by forming an imprinted layer on silica nanoparticle surfaces through a simple one-pot reaction.

SERS-based aptasensor for culture-free detection of Escherichia coli in urinary tract infection diagnosis.

A surface-enhanced Raman scattering (SERS)-based aptasensor was developed for the rapid and sensitive detection of Escherichia coli (E. coli), a major pathogen in urinary tract infections (UTIs). The sensor utilizes magnetic beads embedded with gold nanoparticles (MB-AuNPs) functionalized with capture DNA (cDNA) as both the SERS-active substrate and magnetic separation tool.

Nanoplastics Toxicity Is a Subset of Nanotoxicology, Not a Separate Field.

Nanoplastics toxicity has been framed as an emerging, distinct research area, purportedly addressing a new threat. While this focus has heightened public awareness and influenced the regulation of plastics, isolating nanoplastics toxicity risks inefficiently allocating research resources and hindering sustainable management strategies. Here, using data mining and machine learning, we show that research on nanoplastics toxicity closely mirrors that of engineered nanoparticles, a well-established domain of nanotoxicology.

Dual responsive fluorescent probe for exploring hypochlorite and sulfur dioxide in different heavy metal ions related stress models.

The danger of heavy metal pollution has drawn the global concern of researchers in recent decades, especially multiple heavy metal pollution. Heavy metal pollution induced stress may cause oxidative stress and redox balance disruption in living organism, and further trigger other secondary stresses. Fluorescent imaging analysis is considered to be effective method for real-time visualization of complex bioactive molecules in situ due to their non-destruction, high sensitivity and specificity.

Advancing Whole-Cell Biosensors: Kinetics-Dependent Metabolic SERS Analytics for Pollutant Differentiation and Quantification.

Whole-cell biosensors (WCBs), which detect targeting analytes through cellular responses, have become powerful tools for environmental monitoring. However, existing WCBs often rely on the single-channel low-dimension signal outputs (e.g.

Dual-Pathway Lateral Flow Assay for Rapid and Sensitive SARS-CoV-2 RNA Detection via CRISPR/Cas13a-Mediated SERS.

Reverse transcription-polymerase chain reaction (RT-PCR) has been the gold standard for SARS-CoV-2 detection during the COVID-19 pandemic. However, its requirement for RNA-to-DNA conversion, reliance on centralized laboratory infrastructure, and lengthy turnaround times have limited its application in point-of-care (POC) settings. CRISPR/Cas13a-mediated lateral flow assays (LFAs) have emerged as promising alternatives for direct RNA analysis, yet their two-step workflows introduce procedural complexity and reduce sensitivity.

Sensitive and Facile Detection of Aloin via N,F-CD-Coated Test Strips Coupled with a Miniaturized Fluorimeter.

Aloin, a kind of active phenolic component, is sourced from . Recently, the determination of aloin has received enormous attention, owing to its positive performance (including anti-tumor, antibacterial, detoxification, liver protection, anti-stomach damage, and skin protection activities) and painful side effects (increased carcinogenicity caused by excessive use of aloin) impacting human health. This investigation was inspired by the good fluorescence properties of carbon dots (CDs); CD-based sensors have aroused a great deal of interest due to their excellent sensitivity and selectivity.

Bifunctional phenothiazine-Coumarin probe Tz.3: Visual detection of Ag/ ClO in aqueous media and selective imaging of hypochlorite in living cells.

Excessive exposure of hypochlorite ions (ClO) and silver ions (Ag) lead to significant environmental safety and human health risks. How to quick and accurate detecting of Ag and ClO in aquatic environment seems urgent. Continuation of preliminary work, a novel bifunctional fluorescent probe molecule of Tz.

Differences between the image-derived input functions of the descending aorta and carotid artery in brain pharmacokinetic parametric analysis.

Objectives: This study aimed to quantitatively reveal the difference between the descending aorta IDIF (IDIFa) and carotid artery IDIF (IDIFc) in the task of brain pharmacokinetic parametric analysis.

Methods: 18F-FDG dynamic PET data from 50 patients collected by UIH uEXPLORER PET/CT were used to extract the IDIFa and IDIFc; the Patlak graphical model (PGM) and irreversible 2-tissue compartmental model (2TCM) were applied for brain pharmacokinetic parametric analysis. The Patlak Ki and Vt images yielded by the two types of IDIFs are compared using relative error (RE), while the parameters K1∼k3 and fv were analyzed via paired t-tests to determine the significance of the differences.

Cytokine Storm Induction Linked to Multi-Organ Failure in Fatal Jellyfish Stings.

Fatal jellyfish stings often cause multi-organ failure. Until now, these fatal outcomes are attributed to the direct toxic effects of the venom. Here, a mouse model of delayed jellyfish envenomation syndrome (DJES) is established and showed that venom from Nemopilema nomurai jellyfish can trigger a deadly cytokine storm - a severe inflammatory reaction.

Molecularly imprinted polymers based enrichment and separation for trace analysis in capillary electrophoresis.

Capillary electrophoresis (CE) is well known for its high resolution, rapidity, low sample and reagent consumption, and ability to simply change separation modes, and has been widely used for separation and detection of various analytes important for many fields. One major limitation is the concentration limits of detection, which can be further worsened in complex samples with a significant matrix component. Molecularly imprinted polymers (MIPs) based solid-phase extraction (SPE) and stationary phases (SPs) enjoy great popularity in CE, owing to the recognition specificity, physical robustness, thermal stability, as well as low cost and easy preparation of MIPs.

Co-Self-Assembled Monolayer Enables Sensitive SERS Detection of Nanoplastics via Spontaneous Hotspot Entrapment.

Identification and quantitative analysis of nanoplastics (NPs) in the environment are extremely challenging. Surface-enhanced Raman scattering (SERS) is a promising technique, but conventional solid SERS substrate-based detection faces difficulties such as ensuring NPs make contact with hotspots, dealing with uneven particle distribution, and poor detection repeatability. Herein, we propose a simple and sensitive SERS detection strategy by co-self-assembling silver (Ag) nanoparticles and NPs in a monolayer.

Paper-based SERS chip with adaptive attention neural network for pathogen identification.

High-speed and accuracy identification of pathogens has become increasingly critical in both individual patient care and public health. Artificial intelligence (AI)-assisted surface-enhanced Raman scattering (SERS) biosensors enable simultaneous identification of multiple pathogens. However, there are still problems such as low accuracy and limited diversity in bacterial fingerprints.

Cavity-Like Silver Aggregates-Based Colloidal SERS Microfluidic Platform for Highly Reproducible Online Reaction Process Analysis.

Process analytical technology (PAT) is a key tool in the chemical and biological production industry. However, it is still desirable to develop online PAT enabling rapid and sensitive detection of various reaction intermediates, to meet the requirements of precise and green chemistry. Here these challenges are addressed by developing a cavity-like silver aggregate (Ag cavity)-based colloidal surface-enhanced Raman scattering (SERS) microfluidic platform, which exhibits a reproducible flow detection window, enabling sensitive online monitoring and identification of the organic reaction intermediates of the model flow photochemical reactions.

A molecularly imprinted polymer-based triple-ratio fluorescence nanosensor integrated with a paper-based microfluidic chip for rapid detection of enoxacin.

Enoxacin (ENX), a commonly used fluoroquinolone antibiotic, has raised environmental concerns due to its widespread use and resulting pollution. This study presents a novel approach for the rapid and sensitive detection of ENX in aquatic environments using molecularly imprinted polymer-based triple-ratio fluorescence (MIP-TRF) nanosensor. The nanosensor is constructed based on a photoinduced electron transfer (PET) mechanism, combining the high selectivity of molecular imprinting technology with the high sensitivity and anti-interference capability of triple-ratio fluorescence.

One-pot preparation of anionic ligand-stabilized gold nanoparticles with low SERS background for detecting reaction intermediates under strong oxidative conditions.

Gold nanoparticle-based surface-enhanced Raman scattering (SERS) substrates exhibit better chemical stability compared with silver ones, making them suitable for characterizing reaction intermediates in the presence of strong oxidants such as HO. However, conventional wet-chemistry-synthesized gold nanoparticles often show strong background signals from organic stabilizers, which could overlap and disturb the SERS signals of reaction intermediates and products. In this work, a low-background corrosion-resistant gold-based SERS substrate was prepared a facile one-pot method using anionic ligands as stabilizers, achieving the rapid characterization of the reaction process in the presence of HO.

Detecting microsatellite instability in cancer multiplexed orthogonal gap-enhanced Raman tags.

Microsatellite instability (MSI) is a hallmark of colorectal cancer in immunotherapy, whose phenotypes mainly involve four mismatch repair (MMR) proteins (MlH1, MSH2, MSH6 and PMS2). Since these MMR proteins are highly interdependent, simultaneous detection of these proteins rather than separate detection in cancer is vital to accurately distinguish the MSI phenotypes. In this study, we fabricated four orthogonal gap-enhanced Raman tag (O-GERT) flavors with high sensitivity, superb photostability, and completely separated interference-free signal readouts.

Recent advances in molecular-imprinting-based solid-phase microextraction for determination of pharmaceutical residues.

Pharmaceutical residues usually exist in various complicated matrices at trace levels, but pose potential threats to human health and ecological environment. Recognition and determination of the residues are important and urgent. Therefore, efficient sample pretreatment techniques become a research hotspot for the sensitive and precise determination by chromatography and mass spectrometry.

Surface enhanced Raman scattering tag enabled ultrasensitive molecular identification of Hippocampus trimaculatus based on DNA barcoding.

Rapid and precise DNA barcode-based identification of biological species holds significant potential for pharmaceutical authentication and biomedical diagnostics. Herein, we present a polymerase chain reaction (PCR)-surface-enhanced Raman scattering (SERS) platform that integrates SERS tags for ultrasensitive and fast authentication of Hippocampus trimaculatus, a high-value traditional Chinese medicine (TCM). The SERS tags are composed of gold nanostars, near-infrared cyanine7 Raman reporters and carboxylated polystyrene shells, which achieve single-particle detection sensitivity under 780 nm irradiation.

Preventing hyperacute rejection in a deceased donor kidney transplant recipient.

A 36-year-old, highly sensitized (cPRA 99.99 %) male listed for his third kidney transplant for more than 12 years received a deceased donor offer. The virtual crossmatch (VXM) indicated that the patient had two weak donor-specific antibodies (DSA) to DR10 and DP17 against potential Donor 1, which would correlate with a negative T/B physical crossmatch (PXM).

Simultaneous magnetic purification and detection of transferrin in human serum using an imprinting-based fluorescence sensor by boronate affinity and secondary signal amplification assay.

Transferrin (TRF) is an important glycoprotein for the early detection of disease. However, its rapid isolation and detection are challenging due to its complex structure, composition, and susceptibility to denaturation. Herein, a novel imprinting-based sensor was introduced to purify and detect TRF in human serum simultaneously.

Membrane protected multi-template molecularly imprinted polymer based micro-solid phase extraction coupled with high performance liquid chromatography for analysis of chlorophenols.

Multi-template molecularly imprinted materials (mt-MIPs) were prepared by surface imprinting and sealed into porous nylon membrane as micro-solid phase extraction (μ-SPE) device, coupled with high performance liquid chromatography (HPLC) to simultaneously extract and determine five typical chlorophenols (CPs) in leather matrices. By using well-prepared mesoporous silica modified multiwalled carbon nanotubes (MWCNTs@mSi) as supporting materials, and the mixture solution of five CPs as templates, the imprinted layer containing recognition sites of the CPs were formed via free radical polymerization. The as-prepared mt-MIPs were characterized by SEM, TEM, FT-IR and TGA, and the adsorption properties were investigated including static, dynamic and selective adsorption, showing high adsorption capacity of 39.

Portable on-site colorimetric kit using stable gold nanorod powder for tracing hydrogen sulfide in typical environmental samples.

On-site detection of hydrogen sulfide (HS), a toxic byproduct of microbial decomposition, is essential for environmental surveys, sewage tracing, and occupational safety. While existing gold nanorods (Au NRs)-based colorimetric methods offer portability and sensitivity, their practicality is hindered by the instability of enzymes and colloidal nanoparticles. Herein, we present an enzyme-free, portable HS detection device using Au NRs powder, ensuring highly sensitive and reliable colorimetric detection while enhancing long-term applicability.

Encapsulation strategy based on aggregation-induced emission effect for the dual-emission ratiometric fluorescence detection of tetracycline.

Metal-organic frameworks (MOFs) materials are highly porous and easily modified, and can have great potential for application in rapid fluorescence analysis of pollutants in materials with aggregation-induced emission (AIE) properties. Herein, a dual-emission ratiometric fluorescence nanosensor was constructed based on the copper nanoclusters encapsulated in zeolite imidazole framework-8 (CuNCs@ZIF-8) for visual detection of tetracycline (TC). Obviously, the fluorescence properties of CuNCs@ZIF-8 were highly enhanced by AIE effect under restriction of ZIF-8.