Gut microbiota-mediated causal relations between sleep disorders and stroke: a Mendelian randomization study.

Background: Sleep disorders are closely linked to stroke, while the role of gut microbiota in human health and disease is increasingly recognized. However, the causal relationships between sleep disorders, gut microbiota, and stroke remain unclear.

Objective: This study aimed to investigate the causal relationships between sleep disorders, gut microbiota, and stroke (including its subtypes), as well as the potential mediating role of gut microbiota in the association between sleep disorders and stroke.

Time-series metatranscriptomics reveals differential salinity effects on the methanogenic food web in paddy soil.

Saltwater intrusion and sea level rise (SWISLR) threaten coastal agroecosystems, yet their impact on the methanogenic food web in rice paddies remains virtually unknown. Using "double-RNA" metatranscriptomics (rRNA, mRNA), we investigated salinity effects on the methanogenic community in straw-amended slurries following different preincubation periods. Our results demonstrate that salt stress significantly inhibits methane (CH) production, with the degree of inhibition varying by the community's successional stage.

Human resource development: An empirical study on analyzing the role of human resource development in organizational efficiency.

BackgroundProfessional activity and intellectual knowledge in the field of human resource development are comparatively modern. Due to organizations' strong interest in managing human resources in the face of fierce competition and shifting business conditions, this field of management development has experienced the quickest growth over the past decades.ObjectivesThe purpose of this study is to examine the human resource development practices impacting the employee efficiency and organization effectiveness.

Global Response and Mechanism of Methane Cycling in Wetlands Under Elevated Atmospheric CO and Warming.

Wetland is one of the most significant sources of methane (CH). Although global warming and elevated atmospheric carbon dioxide concentrations (eCO) are expected to affect the CH cycle, the response of CH emission in natural wetland and paddy has been observed to be inconsistent. This variation is likely due to the complex interactions among soil, plant, and microbial processes that regulate CH dynamics, leaving the underlying mechanisms across global studies unknown.

Transcriptome and metabolome analyses reveal new insights into the regulatory mechanism of early embryoless seed development in rice.

Background: Embryo development holds a pivotal position in the ontogenesis and morphogenesis of rice plants, exerting a direct influence on plant growth and ultimately determining rice yield. Glutaredoxin, an abundant oxidoreductase, plays a crucial role across many facets of plant growth. Our research has uncovered that OsGrxC2.

Coevolution and cross-infection patterns between viruses and their host methanogens in paddy soils.

Methanogens play a critical role in global methane (CH) emissions from rice paddy ecosystems. Through the integration of metagenomic analysis and meta-analysis, we constructed a CRISPR spacer database comprising 14 475 spacers derived from 351 methanogenic genomes. This enabled the identification of viruses targeting key methanogenic families prevalent in rice paddies, including , , , , and .

Radiative forcing reduced by early twenty-first century increase in land albedo.

Surface albedo greatly affects how much energy the Earth absorbs. Intensive human activities and accelerated climate change have altered surface albedo across spatial and temporal scales, yet assessments of the effects of land use or land cover (LULC) and snow variations on land surface albedo are scarce at the global scale. As a result, the global land surface albedo dynamics over recent decades and their corresponding radiative forcing to the climate system remain poorly understood.

A novel, covalent broad-spectrum inhibitor targeting human coronavirus M.

Human coronaviruses (CoV) cause respiratory infections that range from mild to severe. CoVs are a large family of viruses with considerable genetic heterogeneity and a multitude of viral types, making preventing and treating these viruses difficult. Comprehensive treatments that inhibit CoV infections fulfill a pressing medical need and may be immensely valuable in managing emerging and endemic CoV infections.

Attofarad-Class Ultra-High-Capacitance Resolution Capacitive Readout Circuits.

In order to meet the application requirements for high-precision and low-noise accelerometers in micro-vibration measurement and navigation fields, this paper presents the design and testing of an ultra-high-capacitance resolution capacitive readout circuit with attofarad-level precision. First, a differential charge amplifier circuit is employed for the first stage of capacitance detection. To suppress noise interference in the circuit, a frequency-domain modulation technique is utilized to mitigate low-frequency noise.

Can large language models facilitate the effective implementation of nursing processes in clinical settings?

Background: The quality of generative nursing diagnoses and plans reported in existing research remains a topic of debate, and previous studies have primarily utilized ChatGPT as the sole large language mode.

Purpose: To explore the quality of nursing diagnoses and plans generated by a prompt framework across different large language models (LLMs) and assess the potential applicability of LLMs in clinical settings.

Methods: We designed a structured nursing assessment template and iteratively developed a prompt framework incorporating various prompting techniques.

A Global Relationship Between Genome Size and Encoded Carbon Metabolic Strategies of Soil Bacteria.

Microbial traits are critical for carbon sequestration and degradation in terrestrial ecosystems. Yet, our understanding of the relationship between carbon metabolic strategies and genomic traits like genome size remains limited. To address this knowledge gap, we conducted a global-scale meta-analysis of 2650 genomes, integrated whole-genome sequencing data, and performed a continental-scale metagenomic field study.

B(MIDA)-directed site-selective intermolecular halofluoroalkylation of alkenes: synthesis of diversely functionalized building blocks.

α-Halo borides are generally constructed Matteson homologation, and the synthesis of both fluorinated and functionalized ambiphilic boronates is challenging and has received inadequate attention. Herein, we describe the -methyliminodiacetyl boronate [B(MIDA)]-directed halogenation of alkenes a complementary sequence involving fluoroalkyl radical addition followed by guided radical-to-metal oxidative addition and C-X reductive elimination. The alkali cation and functional groups in B(MIDA) enable coulombic interaction and weak attraction with halogens, which could weaken the Pd-X bond and assist in C-X bond formation and is verified by DFT calculations.

Increased methane production associated with community shifts towards Methanocella in paddy soils with the presence of nanoplastics.

Background: Planetary plastic pollution poses a major threat to ecosystems and human health in the Anthropocene, yet its impact on biogeochemical cycling remains poorly understood. Waterlogged rice paddies are globally important sources of CH. Given the widespread use of plastic mulching in soils, it is urgent to unravel whether low-density polyethylene (LDPE) will affect the methanogenic community in flooded paddy soils.

Discovery of Pyridine-2-Carboxamides Derivatives as Potent and Selective HPK1 Inhibitors for the Treatment of Cancer.

Hematopoietic progenitor kinase 1 (HPK1) has emerged as an attractive target for immunotherapy due to its critical role in T cell activation and proliferation. The major challenge in developing HPK1 inhibitors lies in balancing kinase selectivity, pharmacokinetic (PK) properties, and therapeutic efficacy. In this study, we report a series of pyridine-2-carboxamide analogues demonstrating strong HPK1 inhibitory activity in enzymatic and cellular assays, along with good kinase selectivity.

Design, Synthesis, and Biological Evaluation of a Series of Spiro Analogues as Novel HPK1 Inhibitors.

Hematopoietic progenitor kinase 1 (HPK1) negatively affects T cell activation and proliferation and is a promising target for immunotherapy. Although HPK1 inhibitors have shown promising efficacy in preclinical models, none have been approved for clinical use. One significant challenge in developing an HPK1 inhibitor is the difficulty in designing a potent inhibitor with good kinase selectivity and pharmacokinetic properties.

Various microbial taxa couple arsenic transformation to nitrogen and carbon cycling in paddy soils.

Background: Arsenic (As) metabolism pathways and their coupling to nitrogen (N) and carbon (C) cycling contribute to elemental biogeochemical cycling. However, how whole-microbial communities respond to As stress and which taxa are the predominant As-transforming bacteria or archaea in situ remains unclear. Hence, by constructing and applying ROCker profiles to precisely detect and quantify As oxidation (aioA, arxA) and reduction (arrA, arsC1, arsC2) genes in short-read metagenomic and metatranscriptomic datasets, we investigated the dominant microbial communities involved in arsenite (As(III)) oxidation and arsenate (As(V)) reduction and revealed their potential pathways for coupling As with N and C in situ in rice paddies.

Discovery of a Novel Macrocyclic Noncovalent CDK7 Inhibitor for Cancer Therapy.

Cyclin-dependent kinase 7 (CDK7) is a key regulator of the cell cycle and transcription, making it a promising target for cancer therapy. Although current CDK7 inhibitors have improved in their selectivity and druglike properties, CDK7 inhibitors have failed to progress through clinical development due to severe gastrointestinal and hematotoxic side effects. To mitigate these limitations, we have developed novel, macrocyclic, noncovalent CDK7 hit compounds and using a macrocyclization platform that has optimized these compounds from SY-5609, a leading clinical asset.

Taxonomic Diversity, Predicted Metabolic Pathway, and Interaction Pattern of Bacterial Community in Sea Urchin .

Bacterial assemblages associated with sea urchin are critical to their physiology and ecology within marine ecosystems. In this study, we characterized the bacterial communities in wild sea urchin captured in Daya Bay, South China Sea. A total of 363 amplicon sequence variants belonging to nine phyla and 141 genera were classified from intestine, body surface, and surrounding seawater samples.

Fulvic Acid Enhances Nitrogen Fixation and Retention in Paddy Soils through Microbial-Coupled Carbon and Nitrogen Cycling.

Fulvic acid, the most soluble and active humic substance, is widely used as an agent to remediate contaminated soils and improve soil fertility. However, the influence of fulvic acid (FA), as a microbial carbon source, on carbon and nitrogen cycles in paddy soils remains elusive. Therefore, to investigate it, an incubation experiment was conducted.

Regularity and correlation analysis of regional lymph node metastasis in nonoperative patients with non-small cell lung cancer based on positron emission tomography/computed tomography images.

Background: Definitive concurrent chemoradiotherapy (CCRT) is the standard treatment for locally advanced, inoperable non-small cell lung cancer (NSCLC). Previous studies have mainly focused on examining local failure and recurrence patterns after surgery and the principles of lymph node metastasis (LNM) in surgical candidates with NSCLC. However, these studies were just only able to guide postoperative radiotherapy (PORT) and the patterns of LNM in patients with resected NSCLC was inadequate to represent that in locally advanced inoperable NSCLC patients for guiding target volume delineation of CCRT.

Discovery of 1(2H)-phthalazinone and 1(2H)-isoquinolinone derivatives as potent hematopoietic progenitor kinase 1 (HPK1) inhibitors.

Although immune checkpoint inhibitors (ICIs) have been a revelation for treating several cancers, an unmet need remains to broaden ICI therapeutic scope and increase their response rates in clinical trials. Hematopoietic progenitor kinase 1 (HPK1) is a negative regulator of T cell activation and has previously been identified as a promising target for immunotherapy. Herein, we report the discovery of a series of HPK1 inhibitors with novel 1(2H)-phthalazinone and 1(2H)-isoquinolinone scaffolds.

The early prognostic value of the 1-4-day BCM/PA trend after admission in neurocritical patients.

The purpose of this study was to investigate early stage dynamic changes in relevant indicators in neurocritical patients to identify biomarkers that can predict a poor prognosis at an early stage (1-4 days after admission). This study retrospectively collected clinical data, inflammatory indicators, and nutritional indicators from 77 patients at the neurology intensive care unit. The 3-month modified Rankin scale score was used as the outcome indicator.