Feasibility and reliability of high-resolution phase-contrast quantitative-flow magnetic resonance imaging for assessing femoral head blood flow: a prospective study.

Background: The early identification of abnormal blood supply to the femoral head is crucial for timely intervention. High-resolution phase-contrast quantitative-flow (Q-flow) magnetic resonance imaging (MRI) is a promising non-invasive technique for quantitatively assessing blood flow to the femoral head. This study aimed to evaluate the feasibility and reliability of Q-flow in quantifying blood flow to the femoral head.

Dual-layer spectral detector computed tomography for adrenal adenoma characterization: radiation dose reduction and quantitative agreement of multiphase virtual noncontrast with true noncontrast imaging.

Background: Computed tomography (CT) is the preferred imaging modality for evaluating adrenal lesions; however, the associated radiation exposure remains a significant concern. Dual-layer spectral detector CT (SDCT)-derived virtual noncontrast (VNC) images may reduce radiation exposure by eliminating dedicated noncontrast scans, yet their agreement with true noncontrast (TNC) imaging remains debated. This study aimed to quantitatively evaluate the agreement and image quality of VNC images [reconstructed from the arterial phase (VNCa) and portal venous phase (VNCp)] compared to TNC images in adrenal adenomas stratified by lipid content, and to assess the radiation dose reduction.

Development of a rapid recombinase polymerase amplification-lateral flow dipstick assay for sensitive detection of duck adenovirus type-3.

Duck adenovirus type-3 (DAdV-3) infections have severe effects on duck health, and rapid detection methods are crucial to reduce the morbidity and mortality associated with this pathogen in clinical practice. In this study, we established, optimized, and validated a novel recombinase polymerase amplification (RPA)-lateral flow dipstick (LFD) assay for the detection of DAdV-3. Next, we established a clinical infection model based on the pathogenicity of the DAdV-3 strain and tested the effectiveness of the RPA-LFD assay in controlling an outbreak of DAdV-3 infection.

Multiparametric diffusion-weighted MRI for renal microstructural assessment in patients with primary aldosteronism at varying direct renin concentrations.

Purpose: To utilize multiparametric diffusion-weighted imaging (DWI) to detect and quantify renal microstructural alterations in patients with primary aldosteronism (PA) at varying direct renin concentrations (DRC), and to explore the correlations between DWI-derived biomarkers and renal function biochemical indices.

Materials And Methods: This study included 50 patients and 32 healthy controls (HCs). Participants underwent 3.

Cardiac structure and function recovery in acromegaly after treatment: insights from cardiac magnetic resonance imaging.

Purpose: Cardiac magnetic resonance imaging (CMRI) provides a detailed method for understanding the specific cardiovascular alterations associated with acromegaly. This study aimed to evaluate the impact of personalized treatment on cardiac structure, function, and myocardial tissue characteristics using CMRI, and to assess the effects of biochemical remission on improving cardiovascular complications in acromegaly patients.

Method: Thirty-nine acromegaly patients were enrolled from July 2020 to February 2023 at West China Hospital of Sichuan University.

Protein β-O-glucosylation by Legionella LtpM through short consensus sequons G-T/S and S-G.

Unlike N-glycosylation, protein O-glycosylation often lacks a strict consensus sequon, making synthesis of homogeneous O-glycoproteins and site-specific engineering of O-glucosylation challenging. Here we identify Legionella effector LtpM as a versatile protein β-O-glucosyltransferase recognizing extremely short two-residue sequons G-T/S and S-G. X-ray crystallography, molecular simulation and biochemical studies together reveal a unique catalytic mechanism: four residues of LtpM (F166, Q167, W228 and K225) serve as 'gatekeepers' above the binding pocket of the uridine diphosphate (UDP)-glucose sugar donor to form a narrow clamp for the substrate proteins, limiting the residue adjacent to serine or threonine to be exclusively glycine.

Discovery of hybrids of bisbibenzyl with furoxan as lysosomotropic agents for the treatment of drug-resistant non-small-cell lung cancer.

Twenty-one novel macrocyclic bisbibenzyl-furoxan hybrids were designed and synthesized as lysosomotropic agents, with subsequent evaluation of their anticancer potential against multidrug-resistant (A549/Taxol, MCF-7/ADR) and parental (A549, MDA-MB-231, MCF-7) cancer cell lines. Compound 15a emerged as the most promising candidate, demonstrating potent antiproliferative activity against paclitaxel-resistant A549/Taxol cells (IC = 0.87 μM) with good selectivity over normal cells.

Invariant chain binds to both major histocompatibility complex I and major histocompatibility complex II in ducks.

Objective: To investigate the intracellular localization and binding of duck invariant chain (Ii) to major histocompatibility complex (MHC) class Iα or MHC class IIβ molecules.

Methods: First, the genes of duck Ii, MHC Iα, and MHC IIβ were amplified by PCR, cloned into expression plasmids, and then sequenced to compare homology between ducks and selected agricultural species. Second, Rosetta or 293T cells were transfected with plasmids encoding Ii, MHC Iα, and MHC IIβ either alone or in combination to interrogate binding following expression.

Wash-Free Isolation and Quantification of Tumor-Derived Exosomes via In Situ-Formed Hydrogel.

The isolation and detection of exosomes as tumor markers are of vital importance for the early diagnosis, therapeutic monitoring, and mechanistic studies of tumors. Here, exosomes derived from breast cancer cells were chosen as model targets, and a wash-free, enzyme-free, handheld mini centrifugation method based on hydrogels was developed to effectively isolate and detect breast cancer exosomes. Dual aptamers (CD63-T1 and EpCAM-T2) were employed to specifically recognize and capture breast cancer exosomes.

Cold argon plasma-modified pea protein isolate: A strategy to enhance ink performance and digestibility in 3D-printed plant-based meat.

Pea protein isolate (PPI) is rich in protein and low in allergenicity, but its poor functionality limits its application in 3D food printing. This study examined the effects of cold argon plasma (CP) pre-treatment of PPI combined with sodium carboxymethyl cellulose on the ink properties of 3D-printed plant-based meat and its digestive characteristics. The results showed that CP treatment significantly increased the apparent viscosity and storage modulus of the PPI-polysaccharide mixture through hydrogen bonding and other molecular interactions, improving the self-supporting ability and printing accuracy of the plant-based meat.

In Situ Carboxylate-Functionalized {MoO}-Based Three-Dimensional Supramolecular Framework as a Copper-Triggered Heterogeneous Catalyst for Alkene Oxidation.

It is of great research value to develop an efficient and stable catalyst that can achieve the oxidation of alkenes to obtain epoxides, which can serve as a multifunctional intermediate with important commercial value. Based on this, a three-dimensional (3D) supramolecular framework H[Cu(Hbdcbpy)(MoO)(HO)] (, Hbdcbpy·2Cl = ,'-bis(3,5-dicarboxybenzyl-4,4'-bipyridine·dichloride) was obtained from carboxylate-functionalized 1D coordination polymers, which were derived from an unusual in situ [MoO] cluster and Cu-electron-deficient carboxylate complex units. The catalytic performance of in the epoxidation of alkenes using TBHP was examined, revealing its remarkable efficiency in the conversion of -cyclooctene, with a high conversion rate of 99% and an outstanding selectivity of 100%.

Y-Shaped Deoxyribonucleic Acid Scaffold Pendulums: A One-Step Electrochemical Sensor.

The challenge of developing sensing platforms for the direct monitoring of targets within complex samples is well-recognized. To address this, a one-step electrochemical sensing detection platform was introduced, featuring an innovative Y-shaped DNA molecular pendulum design. The approach deviated from the conventional molecular pendulum mode by employing a split aptamer instead of a full one, thereby enabling the detection of small molecules and low-molecular-weight proteins.

Reversible engineering of cell membrane receptors based on host-guest recognition for on-demand regulation of cellular behavior.

Cell receptors are key regulators of cellular signaling. However, on-demand reversible engineering of cell receptors to intervene in cellular behavior remains a challenge. Herein, a reversible receptor engineer strategy (SL1/NCDP/Ada) was developed.

Enhancing Analysis of Extracellular Vesicles by Microfluidics.

Extracellular vesicles (EVs) play crucial roles in intercellular communication and hold great promise as biomarkers for noninvasive disease diagnosis. Intensive research efforts have been devoted to discovering the EV subpopulations responsible for specific functions or with enhanced effectiveness as disease markers, through extensive EV purification and content analysis. However, their high heterogeneity in size and cargo composition poses significant challenges for reaching such goals.

Feasibility of Round Window Exposure via External Auditory Canal: Classification and Predictive Landmarks.

Objective: To clarify the feasibility and the anatomical characteristics related to round window exposure via the external auditory canal (EAC) without bony removal.

Methods: Surgical videos and radiological data from 50 adult patients who underwent endoscopic tympanoplasty type I were collected. According to surgical videos, round window niche (RWN) exposure was classified as "Certainly," "Possibly," and "None," and round window membrane (RWM) exposure was classified as "Clear visualization," "Incomplete visualization," "Just perceptible" and "Invisible.

Oxidative stress in the liver of chicken during fowl adenovirus serotype 4 infection.

Hepatitis is a significant pathological manifestation of fowl adenovirus serotype-4 (FAdV-4) infection, which is a crucial factor contributing to the mortality of chickens. The pathophysiology of liver disease is rooted in oxidative stress. The present study aims to investigate the presence of oxidative stress during the liver lesion process in FAdV-4 infection.

A deep learning model for characterizing altered gyro-sulcal functional connectivity in abstinent males with methamphetamine use disorder and associated emotional symptoms.

Failure to manage emotional withdrawal symptoms can exacerbate relapse to methamphetamine use. Understanding the neuro-mechanisms underlying methamphetamine overuse and the associated emotional withdrawal symptoms is crucial for developing effective clinical strategies. This study aimed to investigate the distinct functional contributions of fine-scale gyro-sulcal signaling in the psychopathology of patients with methamphetamine use disorder and its associations with emotional symptoms.

CBCT radiomics features combine machine learning to diagnose cystic lesions in the jaw.

Objective: The aim of this study was to develop a radiomics model based on cone beam CT (CBCT) to differentiate odontogenic cysts (OCs), odontogenic keratocysts (OKCs), and ameloblastomas (ABs).

Methods: In this retrospective study, CBCT images were collected from 300 patients diagnosed with OC, OKC, and AB who underwent histopathological diagnosis. These patients were randomly divided into training (70%) and test (30%) cohorts.

A comprehensive analysis of scRNA-Seq and RNA-Seq unveils B cell immune suppression in the AAV-loaded brain.

The use of AAV vectors for in vivo gene therapy has demonstrated the potential to permanently correct genetic diseases by delivering functional gene copies to the nuclei of affected tissues. AAV vectors, as tools for in vivo gene delivery, are particularly appealing and have shown safety and long-term efficacy in numerous organ-targeted experiments. Nevertheless, employing AAV vectors for gene therapy in the brain faces a notable hurdle in the shape of immune responses, chiefly instigated by the brain's resident immune cells, microglia.

Design, Synthesis, and Biological Evaluation of Marchantin C-NO Donor Hybrids for Overcoming Pgp-Mediated Drug Resistance by Targeting Lysosome.

A series of marchantin C-NO donor hybrids were designed, synthesized, and evaluated for their antitumor activity and . Notably, MC-furoxan hybrid exhibited the best selective inhibitory activity against MCF-7/ADR (IC = 0.024 μM) with 883 times potency compared with MCF-7 cells (IC = 21.

[Left Ventricular Structural and Functional Changes in Obese Subjects With Preserved Left Ventricular Ejection Fraction After Bariatric Surgery: Assessment With Cardiac Magnetic Resonance Imaging].

Objective: To investigate the longitudinal changes in left ventricular (LV) structure and function after bariatric surgery in obese individuals and their relationship with preoperative left ventricular ejection fraction (LVEF) by cardiac magnetic resonance (CMR) imaging.

Methods: We prospectively enrolled 75 obese subjects scheduled for laparoscopic sleeve gastrectomy and 46 age and sex-matched healthy controls (the control group). All subjects underwent CMR examination to obtain LV structural parameters, LVEF, and strain parameters.

Discovery of potent quinone oxidoreductase 2 inhibitors to overcome TRAIL resistance of non-small cell lung cancer.

Resistance to tumor-necrosis-factor-related apoptosis-inducing ligand (TRAIL) of cancer cells is a main obstacle for the chemotherapy. NRH: quinone oxidoreductase 2 (NQO2), known as a chemopreventive target, has emerged as a promising therapeutic target for overcoming TRAIL resistance in non-small cell lung cancer (NSCLC). Here we report the design, synthesis and evaluation of resveratrol analogues as novel selective NQO2 inhibitors, and analogue 20b, with potent NQO2 inhibitory activity (IC = 95 nM) and relatively low cytotoxicity, displayed synergistic lethal effects in combination with TRAIL on TRAIL-resistant NSCLC cells.

The prognostic value of CMR diastolic parameters in patients with cardiac light-chain amyloidosis: a retrospective analysis.

Cardiac light-chain amyloidosis (AL-CA), characterized by the accumulation of amyloid fibers in the myocardium, often results in progressive diastolic dysfunction and adverse clinical outcomes. This study aimed to explore the diastolic characteristics of AL-CA using feature tracking cardiac magnetic resonance (CMR) and determine the prognostic parameters for all-cause mortality in such patients. A total of 102 AL-CA patients who underwent CMR between January 2014 and September 2018 were retrospectively reviewed, participations were follow-up until 2021.

Synergistic Integration of Halide Perovskite and Rare-Earth Ions toward Photonics.

Halide perovskites (HPs), emerging as a noteworthy class of semiconductors, hold great promise for an array of optoelectronic applications, including anti-counterfeiting, light-emitting diodes (LEDs), solar cells (SCs), and photodetectors, primarily due to their large absorption cross section, high fluorescence efficiency, tunable emission spectrum within the visible region, and high tolerance for lattice defects, as well as their adaptability for solution-based fabrication processes. Unlike luminescent HPs with band-edge emission, trivalent rare-earth (RE) ions typically emit low-energy light through intra-4f optical transitions, characterized by narrow emission spectra and long emission lifetimes. When fused, the cooperative interactions between HPs and REs endow the resulting binary composites not only with optoelectronic properties inherited from their parent materials but also introduce new attributes unattainable by either component alone.

Mutant glycosidases for labeling sialoglycans with high specificity and affinity.

Affinity labeling of biomacromolecules is vital for bioimaging and functional studies. However, affinity probes recognizing glycans with high specificity remain scarce. Here we report the development of glycan recombinant affinity binders (GRABs) based on mutant bacterial sialidases, which are enzymatically inactive but preserve stringent specificity for sialoglycan substrates.