Feasibility and reliability of high-resolution phase-contrast quantitative-flow magnetic resonance imaging for assessing femoral head blood flow: a prospective study.

Background: The early identification of abnormal blood supply to the femoral head is crucial for timely intervention. High-resolution phase-contrast quantitative-flow (Q-flow) magnetic resonance imaging (MRI) is a promising non-invasive technique for quantitatively assessing blood flow to the femoral head. This study aimed to evaluate the feasibility and reliability of Q-flow in quantifying blood flow to the femoral head.

Long-term effectiveness and safety of ustekinumab in patients with Crohn's disease: real-world evidence.

Background: Ustekinumab (UST) is an interleukin-12/interleukin-23 receptor antagonist approved for the treatment of Crohn's disease (CD). Although UST has demonstrated good efficacy and safety in CD, long-term real-world data in Chinese patients are relatively scarce.

Methods: A single-center, observational retrospective study was conducted in the First Affiliated Hospital of Anhui Medical University.

Dibutyl phthalate promotes COAD cells growth and migration via the TIMP1/PI3K/AKT signaling axis.

Dibutyl phthalate (DBP), a widely used plasticizer due to its excellent plasticizing properties, is extensively applied in food packaging and medical devices. Accumulating evidence indicates that DBP exposure is closely linked to the proliferation of malignant tumors. However, its role in the progression of colon adenocarcinoma (COAD) remains to be thoroughly investigated.

Amygdala-Dependent Signaling Pathways and Regulating Circuits in Fear Memory.

Fear memory, a profound emotional memory formed in response to threatening events, is vital to the survival and adaptation of individuals. The amygdala, a central hub in the formation, storage, and expression of fear memory, extensively connects with other brain regions, particularly the hippocampus and hypothalamus, allowing it to rapidly integrate sensory information and generate emotional responses. Past research on the regulatory circuits of fear memory has focused on the amygdala, prefrontal cortex, and hippocampus, emphasizing their interactive roles in the formation, consolidation, and extinction of fear memory.

Mating-induced DNA replication factor minichromosome maintenance protein 10 promotes oogenesis in Plutella xylostella.

During oogenesis, the cell cycle becomes arrested during the DNA replication phase and resumes after mating facilitating crucial processes, including vitellogenesis and chorion formation. However, the molecular mechanisms regulating oogenesis in Plutella xylostella (L.) remain largely unexplored.

Overexpression of CTLA-4 and fibronectin, and lower expression of CD137 (4-1BB) is associated with brain metastasis of primary skin melanomas. An analysis of local immune response by digital spatial profiling.

Background: To discover immune response-associated gene expressions related to brain metastases of skin melanomas, we analyzed a group of fifteen primary skin melanomas consisting of five tumors from patients who did not develop systemic metastases (NM); ten from patients with systemic metastasis of which five with (BM), and five without brain involvement (OOM). Regions of interest (ROIs) harboring tumor cells only; immune cells only; or immune cell infiltration in tumor cell regions, were separately analyzed.

Methods: we profiled the tumor immune microenvironment (TIME) of primary skin melanomas by using a panel of 77 immune response-related oligo-nucleotide targets in the GeoMx Digital Spatial profiler (Nanostring Technologies).

The MFGE8/integrin β3 axis mitigates experimental neutrophilic asthma by suppressing NLRP3-Caspase-1 pathway-mediated NETosis.

Background: Neutrophilic asthma, characterized by the relative accumulation of neutrophils in the airways, constitutes a distinct endotype of asthma resistant to corticosteroid and associated with severe and uncontrolled cases. Milk fat globule-EGF factor 8 (MFGE8) is a soluble glycoprotein functioning in phagocytosis, tissue repair, angiogenesis, and the regulation of neutrophil activity. However, the role of this glycoprotein in neutrophilic asthma has not been thoroughly investigated.

Light-Responsive Oxygen Generation from Chlorella Hydrogels for Facial Nerve Injury Recovery: Crosstalk between M1/M2 Macrophages and Schwann Cells.

Facial nerve injury (FNI), hindered by hypoxic microenvironments limiting Schwann cell (SCs) repair potential, remains a therapeutic challenge. We developed light-responsive Chlorella hydrogels (C-Gel) to modulate oxygen release and inflammation. In vitro, light-activated C-Gel enhanced RSC96 SC proliferation, migration, and secretion while reducing reactive oxygen species (ROS), hypoxia-inducible factor-1α (HIF-1α), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6).

Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella.

Insect seminal fluid proteins (SFPs) are primary factors affecting physiology and behavior in both sexes, making them valuable targets for pest control. However, SFPs have not been fully characterized in the Plutella xylostella, a global pest that attacks cruciferous crops. Here, 75 putative SFPs were identified in P.

The impact of career calling on learning engagement: the role of professional identity and need for achievement in medical students.

Background: Career calling, professional identity and the need for achievement are believed to affect the students' learning engagement and effect. However, their regulatory relationship with medical students remains unclear.

Methods: Our study surveyed 1250 medical students through a questionnaire, and the correlation of the study variables was analysed.

Comparative Transcriptome Analysis Reveals Potential Molecular Regulation of Organic Acid Metabolism During Fruit Development in Late-Maturing Hybrid Citrus Varieties.

Late-maturing hybrid citrus is a significant fruit that combines the best traits of both parents and is highly prized for its unique flavor. Not only can organic acids alter the flavor of citrus pulp, but they are also essential for cellular metabolism, energy conversion, and maintaining the acidbase balance in plant tissues. Although organic acids play a key role in the quality formation of citrus fruits, there is still insufficient research on the metabolic processes of organic acids in late-maturing hybrid citrus varieties.

Tissue-matched analysis of MRI evaluating the tumor infiltrating lymphocytes in hepatocellular carcinoma.

Tumor-infiltrating lymphocytes (TILs) play critical roles in the tumor microenvironment and immunotherapy response. This study aims to explore the feasibility of multi-parametric magnetic resonance imaging (MRI) in evaluating TILs and to develop an evaluation model that considers spatial heterogeneity. Multi-parametric MRI was performed on hepatocellular carcinoma (HCC) mice (N = 28).

Observation of stacking engineered magnetic phase transitions within moiré supercells of twisted van der Waals magnets.

Recent demonstrations of moiré magnetism, featuring exotic phases with noncollinear spin order in the twisted van der Waals (vdW) magnet chromium triiodide CrI, have highlighted the potential of twist engineering of magnetic (vdW) materials. However, the local magnetic interactions, spin dynamics, and magnetic phase transitions within and across individual moiré supercells remain elusive. Taking advantage of a scanning single-spin magnetometry platform, here we report observation of two distinct magnetic phase transitions with separate critical temperatures within a moiré supercell of small-angle twisted double trilayer CrI.

Arginine Biosynthesis Mediates Wulingzhi Extract Resistance to Busulfan-Induced Male Reproductive Toxicity.

Busulfan, an indispensable medicine in cancer treatment, can cause serious reproductive system damage to males as a side effect of its otherwise excellent therapeutic results. Its widespread use has also caused its accumulation in the environment and subsequent ecotoxicology effects. As a Chinese medicine, Wulingzhi (WLZ) has the effects of promoting blood circulation and improving female reproductive function.

Molecular Changes Implicate Angiogenesis and Arterial Remodeling in Systemic Sclerosis-Associated and Idiopathic Pulmonary Hypertension.

Background: Pulmonary hypertension (PH) is a common complication of systemic sclerosis (SSc) and a leading cause of mortality among patients with this disease. PH can also occur as an idiopathic condition (idiopathic pulmonary arterial hypertension). Investigation of transcriptomic alterations in vascular populations is critical to elucidating cellular mechanisms underlying pathobiology of SSc-associated and idiopathic PH.

Sensing spin wave excitations by spin defects in few-layer-thick hexagonal boron nitride.

Optically active spin defects in wide bandgap semiconductors serve as a local sensor of multiple degrees of freedom in a variety of "hard" and "soft" condensed matter systems. Taking advantage of the recent progress on quantum sensing using van der Waals (vdW) quantum materials, here we report direct measurements of spin waves excited in magnetic insulator YFeO (YIG) by boron vacancy [Formula: see text] spin defects contained in few-layer-thick hexagonal boron nitride nanoflakes. We show that the ferromagnetic resonance and parametric spin excitations can be effectively detected by [Formula: see text] spin defects under various experimental conditions through optically detected magnetic resonance measurements.

Single-cell epigenomic dysregulation of Systemic Sclerosis fibroblasts via CREB1/EGR1 axis in self-assembled human skin equivalents.

Systemic sclerosis (SSc) is an autoimmune disease characterized by skin fibrosis, internal organ involvement and vascular dropout. We previously developed and phenotypically characterized an 3D skin-like tissue model of SSc, and now analyze the transcriptomic (scRNA-seq) and epigenetic (scATAC-seq) characteristics of this model at single-cell resolution. SSc 3D skin-like tissues were fabricated using autologous fibroblasts, macrophages, and plasma from SSc patients or healthy control (HC) donors.

Research on distributionally robust energy storage capacity allocation for output fluctuations in high permeability wind and solar distribution networks.

This paper presents a novel approach to addressing the challenges associated with energy storage capacity allocation in high-permeability wind and solar distribution networks. The proposed method is a two-phase distributed robust energy storage capacity allocation method, which aims to regulate the stochasticity and volatility of net energy output. Firstly, an energy storage capacity allocation model is established, which considers energy storage's investment and operation costs to minimize the total cost.

Prediction of immunocyte infiltration and prognosis in postoperative hepatitis B virus-related hepatocellular carcinoma patients using magnetic resonance imaging.

Background: The immune microenvironment (IME) is closely associated with prognosis and therapeutic response of hepatitis B virus-related hepatocellular carcinoma (HBV-HCC). Multi-parametric magnetic resonance imaging (MRI) enables non-invasive assessment of IME and predicts prognosis in HBV-HCC. We aimed to construct an MRI prediction model of the immunocyte-infiltration subtypes and explore its prognostic significance.

Long non-coding RNA H19X as a regulator of mononuclear cell adhesion to the endothelium in systemic sclerosis.

Objective: To define the functional relevance of H19 X-linked (H19X) co-expressed long non-coding RNA (lncRNA) in endothelial cell (EC) activation as a key process in SSc vasculopathy.

Methods: H19X expression in SSc skin biopsies was analysed from single-cell RNA sequencing (scRNA-seq) data. Differential expression and pathway enrichment analysis between cells expressing (H19Xpos) and non-expressing H19X (H19Xneg) cells was performed.

Sast1-mediated manifold effects inhibit Plutella xylostella fertility.

Background: Plutella xylostella (Linnaeus) is a destructive pest of cruciferous crops due to its strong reproductive capacity and extensive resistance to pesticides. Seminal fluid proteins (SFPs) are the main effective factors that determine the reproductive physiology and behaviour of both sexes. Although an increasing number of SFPs have been identified, the effects of astacins in SFPs on agricultural pests have not yet been reported.