Long-chain fatty acids promote ATP production in post-thaw boar sperm through mitochondrial β-oxidation.

Due to the current limitations of boar semen cryopreservation systems, the effective restoration of sperm quality following thawing remains a significant challenge. This study investigates whether post-thaw boar sperm can uptake exogenous long-chain fatty acids (LCFAs) and utilize them for ATP generation, thereby sustaining linear motility and enhancing sperm vitality. Boar semen was diluted in extender solutions supplemented with varying concentrations of a lipid mixture (0, 0.

Neoadjuvant chemoradiotherapy with capecitabine and irinotecan guided by UGT1A1 status in patients with locally advanced rectal cancer: 5-year update of the CinClare trial.

Background: The optimal regimen and chemotherapy intensity are still under investigation for neoadjuvant treatment of locally advanced rectal cancer (LARC). The CinClare trial has demonstrated improved pathologic complete response (pCR) with the addition of irinotecan to neoadjuvant chemoradiotherapy (CRT) guided by uridine diphosphate glucuronosyltransferase 1A1 (UGT1A1) genotype in LARC. Here, we report the 5-year follow-up outcomes of the CinClare study.

Polarity-Switching Photoelectrochemical Biosensor Enabled by Metal/Semiconductor Nanostructures for Ultrasensitive MicroRNA Detection.

Developing efficient and accurate photoelectrochemical (PEC) sensing strategies to eliminate potential false positive or negative signals is crucial for practical applications. In this work, we report a PEC sensing strategy based on CuO nanoparticle-induced photocurrent polarity switching in a heterostructure of InP/ZnS quantum dots (QDs) combined with PdPt nanospheres (InP/ZnS@PdPt). The PdPt nanospheres not only provide versatile support for loading InP/ZnS QDs but also enable a 10-fold enhancement in the PEC activity of the InP/ZnS@PdPt compared to InP/ZnS QDs, attributed to the combined influence of localized surface plasmon resonance and the Schottky junction.

Harnessing dual-channel probiotics to synergistically correct intestinal and vaginal dysbiosis after antibiotic disruption.

Antibiotics are widely used to treat infectious diseases, yet antibiotic therapy has been shown to disrupt symbiotic microbiota. Notably, the dosage and duration of antibiotic use for specific infections may exert detrimental effects on microbiota in non-infected sites. Here, we propose a dual-channel probiotic delivery strategy to address gut and vaginal dysbiosis caused by antibiotic therapies.

A case report of dermatofibrosarcoma protuberans with osteolytic.

Background: Dermatofibrosarcoma protuberans (DFSP) is a rare, low-to-intermediate grade malignant soft tissue sarcoma that is prone to local recurrence after surgery but rarely metastasizes to distant sites. However, in this case, the tumor metastasized to the humerus and presented as a solitary lesion.

Case Presentation: A 42-year-old male patient presented with pain and limited mobility in the left upper limb, with a history of surgery for DFSP in the popliteal fossa.

The dual role of Piezo1 in tumor cells and immune cells: a new target for cancer therapy.

Piezo1, a mechanosensitive ion channel, plays a pivotal and multifaceted role in tumor progression, immune evasion, and therapeutic resistance by transducing extracellular mechanical stimuli-such as matrix stiffness and fluid shear stress-into intracellular calcium influx. In tumor cells, Piezo1 promotes proliferation, invasion, and metastasis by activating oncogenic signaling and contributes to an immunosuppressive TME through regulation of cancer-associated fibroblasts (CAFs) and extracellular matrix (ECM) remodeling. In the immune compartment, Piezo1 integrates mechanical cues with metabolic and epigenetic reprogramming to orchestrate the functions of T cells, macrophages, and natural killer (NK) cells.

[Effect of transcutaneous phrenic nerve stimulation in preventing ventilator-induced diaphragmatic dysfunction in invasive mechanically ventilated patients].

Objective: To explore the preventive effect of transcutaneous phrenic nerve stimulation on ventilator-induced diaphragmatic dysfunction (VIDD) in patients requiring invasive mechanical ventilation.

Methods: A randomized controlled trial was conducted. The patients requiring invasive mechanical ventilation admitted to the intensive care unit (ICU) of Jiaxing First Hospital from November 2022 to December 2023 were enrolled.

[Prediction of Heavy Metal Concentrations in PM in the Agricultural Area of Yangtze River Delta Region Based on Machine Learning].

Heavy metals in PM can considerably impact air quality, human health, and the ecological environment. However, studies on heavy metals in PM in agricultural areas are relatively limited. In this study, observational data on heavy metal concentrations in PM in the Yangtze River Delta Region from 2000 to 2020 were collected.

An integrated medical-care wound management strategy in patients with hard-to-heal lower extremity ulcers undergoing negative pressure wound therapy.

Objective: To explore the clinical effect of an integrated medical‑care wound management strategy in nursing care for patients undergoing negative pressure wound therapy (NPWT) for hard‑to‑heal (chronic) lower extremity ulcers.

Method: Patients admitted to Fujian Provincial Hospital, China, from January to December 2022 were assigned to either an experimental group or a control group. Patients in the experimental group received nursing care under the guidance of an integrated medical care wound management strategy, while patients in the control group received clinical primary nursing care.

Electroacupuncture versus conventional acupuncture of scalp motor area for post-stroke wrist dyskinesia and its effect on muscle function: a randomized, controlled clinical trial.

Objective: To evaluate the efficacy of electroacupuncture (EA) at scalp motor area for treating post-stroke wrist dyskinesia and its influence on the function of wrist movement-related agonistic muscles.

Methods: A randomized, single-blind, controlled clinical trial was conducted. Sixty-six patients undergoing post-stroke wrist dyskinesia were enrolled and randomized 1 : 1 to EA or control group.

NIR light-driven transformable liquid metal-based nanovaccines restrain postoperative colorectal cancer recurrence.

Cancer vaccines mount specific immune memory responses and hold great potential in suppressing postoperative colorectal cancer (CRC) recurrence. However, undesired lymph node trafficking and antigen cross-presentation hamper clinical translation of nanovaccines. Here, we propose a controllable transformable nanovaccine grounded on thermal fusion feature of liquid metal (LM) nanoparticles against postoperative CRC recurrence.

TGF-β-activated NK-92 cell therapy for esophageal squamous cell carcinoma.

Esophageal squamous cell carcinoma (ESCC) is the dominant histological type of esophageal cancer in China, with a poor outcome and limited treatment options. The cytotoxicity of natural killer (NK) cells, independent of specific antigen recognition, renders them a promising candidate for cell-based immunotherapy of cancers. However, immunosuppressive cytokines, particularly TGF-β, are highly expressed in the ESCC tumor microenvironment and exert strong inhibitory effects on NK cells.

A potent GalNAc-siRNA drug, RBD1016, leads to sustained HBsAg reduction and seroconversion in mouse models of HBV infection.

Potent small interfering RNA (siRNA) drugs that could achieve hepatitis B surface antigen (HBsAg) seroconversion and functional cure are currently lacking. The purpose of this study is to evaluate the therapeutic potential of RBD1016, a novel GalNAc-conjugated siRNA drug targeting transcripts of hepatitis B virus (HBV), in animal models. RBD1016 was designed to target X gene of HBV (HBx) and showed strong activities on HBV DNA and HBsAg for multiple HBV genotypes and nucleoside-resistant viral strains in studies.

The clinical effective of Baduanjin rehabilitation training on limb motor function, daily life, and quality of life in elderly patients with hemiplegia after stroke.

This study explores the clinical effects of Baduanjin rehabilitation training on limb motor function, daily living ability, and quality of life in elderly patients with hemiplegia after stroke. Clinical data of 185 elderly patients with hemiplegia after stroke in our hospital from January 2020 to December 2023 were retrospectively analyzed. According to intervention strategy, patients were divided into observation group (n = 75, Baduanjin rehabilitation training in addition to the conventional training therapy) and control group (n = 110, conventional rehabilitation training therapy).

Transient receptor potential (TRP) channels in hair follicle biology: Physiological significance and translational relevance.

Transient receptor potential (TRP) channels are a family of versatile ion channels that act as sensor for a wide range of chemical and physical stimuli. These cation-permeable channels are widely expressed across various tissues and play pivotal roles in numerous biological processes. Studies have demonstrated that six specific TRP channels namely TRPV1, TRPV3, TRPV4, TRPM5 TRPML1, and TRPML3 are actively involved in hair follicle (HF) cell proliferation, modulation of hair growth-related signaling pathways, regulation of hair cycle progression, and control of hair growth and pigmentation in both humans and mice.

YAP1 promotes adipogenesis by regulating the negative feedback mechanism of the Hippo signaling pathway via LATS2.

Adipose-derived mesenchymal stem cells (ADSCs) represent a readily accessible and important source of mesenchymal stem cells (MSCs) capable of multilineage differentiation. The Hippo signaling pathway effector has emerged as a pivotal regulator of stem cell fate, yet the specific molecular mechanism by which it modulates lipogenic differentiation of ADSCs has not been clearly defined. In this study, goat ADSCs (gADSCs) isolated from Albas goats in Inner Mongolia were used to investigate the role of YAP1 in adipogenic differentiation.

Biological potential and mechanisms of Brain-Expressed X-linked family proteins in cancers: an updated review.

Background: Brain-Expressed X-linked (BEX) family proteins is a relative younger protein family in human being, with limited understanding of its pathophysiological functions in health and disease. So far, The human BEX family consists of five members including BEX1-5, which have highly homologous and different subcellular localization, expression patterns and proteasome degradation. Previous research found that BEX family proteins play an important role in p75NTR signal pathway which closely connected with neuron differentiation and regeneration.

Aerobic exercise strategies for anxiety and depression among children and adolescents: a systematic review and meta-analysis.

Objective: This study aimed to investigate the effects of exercise intensity, frequency, session duration, and intervention period in aerobic exercise programs on alleviating depression and anxiety symptoms among children and adolescents. The objective of this study is to develop suitable aerobic exercise plans for these individuals.

Methods: All articles published between the database inception year and November 2024 were obtained from PubMed, Scopus, and Web of Science.

Testicular torsion after laparoscopic appendectomy: salvage by spermatic cord heparin flush and testicular decompression - a case report and literature review.

Background: Testicular torsion is defined as an acute scrotal emergency that occurs in adolescents. It requires intervention within 6 h to avoid necrosis due to ischemia of the testis caused by seminal torsion. The etiology of testicular torsion is mostly associated with anatomical abnormalities, such as pendulum malformation.

SAFE method for dispersion characteristics of fluid-saturated porous media with arbitrary cross sections.

Understanding the dispersion characteristics of guided waves is crucial for the application of ultrasonic guided wave method in fluid-saturated porous media. In this paper, the Semi-Analytical Finite Element (SAFE) method is applied to analyze the dispersion characteristics in fluid-saturated porous media. We derive the SAFE equations for fluid-saturated porous media and reformulate them into the standard Finite Element (FE) eigenvalue form that can be solved using COMSOL Multiphysics.

Targeting RAGE with Nanobodies for Molecular Imaging of Cancers and Alzheimer's Disease.

The receptor for advanced glycation end products (RAGE) is a multifunctional cell surface receptor implicated in aging and the progression of chronic diseases, including cancer and Alzheimer's disease. Its interaction with advanced glycation end products (AGEs) promotes cellular stress and inflammation, underscoring the diagnostic and therapeutic relevance of targeting RAGE. In this study, we explored the potential of nanobodiessingle-domain antibodies known for high specificity, strong affinity, and deep tissue penetrationas molecular tools for RAGE-targeted applications.

Role of UVA-driven cellular senescence via mTOR activation in dihydrotestosterone-induced hair loss in androgenetic alopecia mouse model.

Background: Androgenetic alopecia (AGA) is one of the most common forms of hair loss, and recent studies suggest that dihydrotestosterone (DHT)-induced senescence of dermal papilla cells (DPCs) plays a crucial role in its pathogenesis. Clinically, we previously observed an overlap between areas exposed to ultraviolet (UV) radiation and regions affected by androgenetic hair loss. However, the relationship between UVA radiation and AGA onset remains unclear.

Mechanism of Core Browning in Different Maturity Stages of 'Yali' Pears During Slow-Cooling Storage and PbRAV-Mediated Regulation.

This study investigated the impact of slow cooling on browning and fruit quality at three maturity stages (early, mid and late). Slow cooling reduced core browning in early/mid-harvest pears, as the browning indexes of early-, middle- and late-harvested 'Yali' pears at 60 d were 0.13, 0 and 0.

Inhibition of Factor XI Using RBD4059: A Novel GalNAc-siRNA With Potent and Durable Antithrombotic Effects.

Coagulation factor XI (FXI) is an attractive target for novel antithrombotic drugs because of its minor role in hemostasis, which may address the current unmet medical need regarding a powerful anticoagulant with low bleeding risk. Here, we present RBD4059, the first FXI-targeting GalNAc-siRNA molecule to reach the clinical stage of development. In this study, RBD4059 demonstrated potent FXI inhibition with long duration in mice and monkeys, antithrombotic effects in mouse thrombosis models, and no prolonged bleeding in a mouse tail bleeding model.

Surgical approaches to varicocele: a systematic review and network meta-analysis.

Surgical methods for varicocele remain controversial. This study intends to evaluate the efficacy and safety of different surgical approaches for treating varicocele through a network meta-analysis (NMA). PubMed, Embase, Cochrane, and Web of Science databases were thoroughly searched.