Autofocus method based on frequency domain cross-correlation in a structured illumination microscopy.

Precise focusing is a challenge for live-cell super-resolution structured illumination microscopy (SR-SIM), especially in the case of low signal-to-noise ratio of raw images. Here, we propose an integrated autofocus algorithm (IT-SICC) that combines frequency domain cross-correlation with an Improved Tenengrad algorithm to achieve high-precision autofocus. Due to leveraging the prior characteristics of the SIM imaging fringe pattern, the IT-SICC outperforms existing image-based autofocus methods in terms of focusing accuracy, noise robustness, and independence from morphological features.

The clinical effective of Baduanjin rehabilitation training on limb motor function, daily life, and quality of life in elderly patients with hemiplegia after stroke.

This study explores the clinical effects of Baduanjin rehabilitation training on limb motor function, daily living ability, and quality of life in elderly patients with hemiplegia after stroke. Clinical data of 185 elderly patients with hemiplegia after stroke in our hospital from January 2020 to December 2023 were retrospectively analyzed. According to intervention strategy, patients were divided into observation group (n = 75, Baduanjin rehabilitation training in addition to the conventional training therapy) and control group (n = 110, conventional rehabilitation training therapy).

Synthesis of helical carbon nanotubes wrapped around NiPO nanoparticle-incorporated carbon nanoribbons as an efficient electrocatalyst for the hydrogen evolution reaction.

Helical CNTs wrapped around NiPO nanoparticle-incorporated carbon nanoribbons were prepared by nickel-catalysis and phosphatization. The obtained highly conductive nanocomposite with an active Ni-O-P structure and appropriate Gibbs free energy for adsorbing protons was applied as an advanced electrocatalyst for the HER and delivered comparable electrocatalytic performance to commercial Pt/C in acidic solution.

Comparison of a novel endoscopic approach versus laparoscopic deroofing for symptomatic simple hepatic cysts.

Background: Laparoscopic hepatic cyst deroofing (LHCD) stands as the preferred treatment modality for simple hepatic cysts (SHCs), but it has limitations. This study aimed to introduce endoscopic transgastric hepatic cyst deroofing (ETGHCD) as a minimally invasive alternative and to compare its safety and efficacy with LHCD in treating SHCs.

Methods: We retrospectively enrolled 10 patients with 15 symptomatic SHCs treated by ETGHCD and 53 patients with 72 SHCs treated by LHCD at Shanghai General Hospital from June 2021 to August 2024.

Identification of lipid metabolism-related genes in dapagliflozin treated rats with diabetic cardiomyopathy by bioinformatics.

Background: Diabetic cardiomyopathy (DCM) is a heart disease caused by the metabolic disorders of glucose and lipids associated with diabetes, leading to heart failure and death in diabetic patients. Dapagliflozin (DAPA) serves as a treatment for managing blood glucose levels in individuals with type 2 diabetes mellitus (DM). However, the specific mechanisms by which DAPA treats DCM are not yet fully understood.

Isotropic spectrum optimization for enhancing image fidelity in super-resolution structured illumination microscopy.

Super-resolution structured illumination microscopy (SR-SIM) performs spectral expansion of high-frequency information encoded in stripe patterns. However, using a limited number of pattern orientations (typically three) results in a petal-like frequency spectrum, leading to structural and intensity fidelity degradation in reconstructed images. In this Letter, we propose an integrated spatial-frequency domain SIM reconstruction method that enables isotropic spectrum expansion, called ISO-SIM.

Structural Reconstruction via Carbon Nanotube Spatially Confined Metal Catalysis: A Morphology-Controlled Approach to Convert Polycyclic Aromatic Hydrocarbon into Carbon Nanofibers for Highly Active Anodes in Li-Ion Batteries.

By a carbon nanotube (CNT) spatially confined metal-catalyzed structural reconstruction, carbon nanofibers (CNFs) with a hollow, hollow-solid, solid graphite core, and CNT shell are prepared using nitrogen heterocycle (NHC) and polycyclic aromatic hydrocarbon (PAH) as carbon sources. The formation mechanism of CNFs with oriented graphene layers and enlarged intergraphene spacing is studied by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and selected area electron diffraction analysis. It revealed that this one-dimensional nanoconfined metal-catalyzed carbon rearrangement is totally different from the reported spatially localized metal-catalyzed graphitization of electrospun polymer and nanocasted carbohydrate nanofibers, as the graphene orientation, cavity volume, and interlayer distance of CNFs can be controlled by the carbon concentration-related competitive metal-catalyzed tip growth of latitudinal and longitudinal graphene layers from NHC and PAH.

WNT11 Promotes immune evasion and resistance to Anti-PD-1 therapy in liver metastasis.

Liver metastasis (LM) poses a significant challenge in cancer treatment, with limited available therapeutic options and poor prognosis. Understanding the dynamics of tumor microenvironment (TME) and immune interactions is crucial for developing effective treatments. We find that WNT11 promoted CD8 T-cell exclusion and suppression, which was correlated with poor prognosis in LM.

Syringin inhibits the crosstalk between macrophages and fibroblast-like synoviocytes to treat rheumatoid arthritis via PDE4.

Background: Syringin (SRG) is well-known for its anti-inflammatory effects. However, its pharmacological mechanisms against rheumatoid arthritis (RA) are not fully understood.

Materials And Methods: We assessed the anti-RA effects of SRG using a collagen-induced arthritis (CIA) rat model.

regulates melanocortin 4 receptor transcription and energy homeostasis.

Disruption of hypothalamic melanocortin 4 receptors (MC4Rs) causes obesity in mice and humans. Here, we investigated the transcriptional regulation of in the hypothalamus. In mice, we show that the homeodomain transcription factor Orthopedia (OTP) is enriched in MC4R neurons in the paraventricular nucleus (PVN) of the hypothalamus and directly regulates transcription.

Multiomic characterization, immunological and prognostic potential of SMAD3 in pan-cancer and validation in LIHC.

SMAD3, a protein-coding gene, assumes a pivotal role within the transforming growth factor-beta (TGF-β) signaling pathway. Notably, aberrant SMAD3 expression has been linked to various malignancies. Nevertheless, an extensive examination of the comprehensive pan-cancer impact on SMAD3's diagnostic, prognostic, and immunological predictive utility has yet to be undertaken.

The association between blood vitamin E and blood pressure in an adult population with and without diabetes mellitus.

Aims: Previous studies on the association between vitamin E and blood pressure (BP) levels are controversial. Our study aimed to evaluate the association between blood vitamin E (alpha-tocopherol and gamma-tocopherol) levels and systolic and diastolic BP in an adult population with diabetes and without diabetes.

Methods: Our study data were obtained from a biomarker project of the Midlife in the United States (MIDUS) study.

Effects of material and structure types on drifting speed of fish aggregation devices (FADs) in tuna purse seine fishery.

The drifting speed and trajectory of fish aggregation devices (FADs) influence tuna aggregation behavior. Based on expertise, fishermen modify FAD structures to slow down drifting speed, but few studies quantify material and structure effects on this speed. To address this, 14 different types of FAD models were tested under 5 different current velocities in a flume tank and compared drifting using pairwise Wilcoxon tests.

Rational Synthesis of Spongy FeN@N-Doped Carbon Nanorods with Controlled Topography and Porosity for Enhanced Energy Storage Anodes in Lithium-Ion Batteries.

Iron nitrides with the merits of high theoretical capacities, cost-effectiveness, and good electronic/ionic conductivity have been recognized as attractive anode candidates for lithium-ion batteries (LIBs). Carbon compositing, pore engineering, and nanostructure construction have proved to be effective strategies to prepare high-performance metal nitride anodes for LIBs. Herein, we synthesized a series of FeN-embedded and N-doped carbon nanorods (FeN@NCNR) with a hierarchical porous system and controllable topography by metal-catalyzed graphitization-nitridization of the Fe(III)-triazole framework (Fe-MOF) and thermal evaporation of the triblock copolymer F127 template assembled in Fe-MOF via hydrogen bonding interaction, followed by the air oxidation and urea-assisted ammonolysis processes.

Burden of digestive system diseases in China and its provinces during 1990-2019: Results of the 2019 Global Disease Burden Study.

Background: Evaluating the impact of digestive system diseases is vital for devising effective prevention strategies. However, comprehensive reports on the burden of digestive system diseases in China are lacking. Our study aimed to provide an overview of the burden and trends of digestive system diseases from 1990 to 2019 in China and its provinces.

HIF-1α protects nucleus pulposus cells from oxidative stress-induced mitochondrial impairment through PDK-1.

The pathogenesis of intervertebral disc degeneration (IVDD) involves complex signaling networks and various effector molecules, and our understanding of the pathogenesis of IVDD is limited. Hypoxia inducible factor-1α (HIF-1α) is closely related to IVDD, and there is excessive oxidative stress concurrent with IVDD. In this study, we found that HIF-1α could protect nucleus pulposus cells from excessive oxidative stress by reversing the imbalance between oxidants and antioxidants and thus mitigating the oxidative stress-induced mitochondrial impairment.

Altered biliary microbial and metabolic profile reveals the crosstalk between NAFLD and cholelithiasis.

Background: The relationship between non-alcoholic fatty liver disease (NAFLD) and cholelithiasis is intricate, with alterations in the microenvironment potentially mediating this interplay. Thus, this study aimed to explore the biliary microbiota and metabolites of patients with cholelithiasis and detect changes induced by comorbid NAFLD.

Methods: In this study, 16S rRNA gene sequencing and metabolome analysis were performed on biliary samples collected from 35 subjects.

Methylglyoxal induces endothelial cell apoptosis and coronary microvascular dysfunction through regulating AR-cPLA signaling.

Objective: Since diabetic patients with coronary microvascular dysfunction (CMD) exhibit high cardiac mortality and women have higher prevalence of non-obstructive coronary artery disease than men, we tried to expand the limited understanding about the etiology and the sex difference of diabetic CMD.

Approach And Results: Accumulated methylglyoxal (MGO) due to diabetes promotes vascular damage and it was used for mimicking diabetic status. Flow cytometry analysis and isometric tension measurement were performed to evaluate coronary artery endothelial injury.

Sanguinarine Induces Necroptosis of HCC by Targeting PKM2 Mediated Energy Metabolism.

Backgrounds: Abnormal metabolism is the hallmark of hepatocellular carcinoma. Targeting energy metabolism has become the major focus of cancer therapy. The natural product, sanguinarine, displays remarkable anti-tumor properties by disturbing energy homeostasis; however, the underlying mechanism has not yet been elucidated.

Development of esophagogastroduodenoscopy in China: results from the national census in 2013 and 2020.

Background: Given the significant burden of upper digestive diseases, there has been a substantial increase in the utilization of esophagogastroduodenoscopy (EGD) in China from 2012 to 2019. The objective of this study is to investigate the development, practice, and factors influencing the widespread use of EGD during this period.

Methods: Two national censuses were conducted among all hospitals in mainland China that perform gastrointestinal endoscopy.

Inhibition of CIRBP represses the proliferation and migration of vascular smooth muscle cells via inhibiting Rheb/mTORC1 axis.

The excessive migration and proliferation of vascular smooth muscle cells (VSMCs) plays a vital role in vascular intimal hyperplasia. CIRBP is involved in the proliferation of various cancer cells. This study was aimed to explore the role of CIRBP in the proliferation and migration of VSMCs.

Comprehensive prognostic and immunological analysis of Cullin2 in pan-cancer and its identification in hepatocellular carcinoma.

Background: As a member of the Cullin family, Cullin2 (CUL2) is involved in the development and spread of different types of cancers. However, the precise role of CUL2 in human cancer remains largely elusive.

Methods: In this study, various databases were applied to observe the CUL2 expression.

A safe and effective endoscopic treatment method for simple hepatic cysts (with video).

Symptomatic simple hepatic cysts require treatment, with several guidelines recommending laparoscopic deroofing. However, cysts located in the posterosuperior segments are considered poor candidates for this procedure. Gastrointestinal endoscopes are more flexible and able to reach less accessible areas than laparoscopes.