NegSPQ: Similar Patient Query Based on Negative Representation of Genomic Data.

With the development of genome sequencing technology, genomic data have been widely collected and used in real-world scenarios, such as genomic medicine and similar patient query (SPQ) cases, based on similarity comparisons of genomic sequences. However, genomic data are unique for every person and contain a large amount of sensitive information involving personal privacy. Therefore, determining how to protect privacy while utilizing genomic data has become a key issue.

Eradication of Intracellular Persisters via On-Site Antibiotic Delivery by Poly(amino acid) Nanoparticles.

Intracellular persisters are a dormant bacterial subpopulation responsible for chronic and recurrent infections due to their ability to evade antibiotic treatment within host cells. However, effective strategies for eliminating these intracellular pathogens remain limited. Herein, we proposed a versatile poly(amino acid)-based platform, F(AM), for the effective eradication of intracellular persisters via on-site antibiotic delivery.

Plasma ddPCR for etiological diagnosis of focal bacterial infections.

Background: Plasma droplet digital polymerase chain reaction (ddPCR) has been used for pathogen detection and has shown good diagnostic value, but no studies have yet demonstrated its application in focal infections. Herein a pilot study using plasma ddPCR to diagnose focal infection is discussed.

Methods: Eight patients with a diagnosed focal infection who underwent plasma ddPCR for bacterial detection between 2021 and 2022 in Sir Run Run Shaw hospital were enrolled in this retrospective single-center pilot study.

Taurine attenuates LPS-induced pro-inflammatory cytokine production in IPEC-J2 cells by inhibiting the TLR4-MyD88/TRIF signaling pathway.

Post-weaning diarrhea (PWD) in piglets poses a significant challenge to global swine production, primarily due to weaning stress leading to intestinal barrier dysfunction. Recent studies have identified taurine, a conditionally essential amino acid, as a crucial modulator of innate immune responses. In this study, we utilized lipopolysaccharide (LPS)-treated porcine intestinal epithelial cells (IPEC-J2) to investigate the anti-inflammatory mechanisms of taurine.

Fear of cancer recurrence and influencing factors in elderly patients with gastric cancer undergoing laparoscopic radical surgery.

Background: Fear of cancer recurrence (FCR) is a common psychological problem among patients with cancer, especially elderly patients. For patients with gastric cancer (GC) who have undergone treatments such as surgery, FCR may seriously affect their quality of life and psychological well-being.

Aim: To evaluate the FCR in elderly patients with GC undergoing laparoscopic radical surgery in Hefei City and to explore its related influencing factors.

Forecast of Aging of PEMFCs Based on CEEMD-VMD and Triple Echo State Network.

Accurately forecasting the degradation trajectory of proton exchange membrane fuel cells (PEMFCs) across a spectrum of operational scenarios is indispensable for effective maintenance scheduling and robust health surveillance. However, this task is highly intricate due to the fluctuating nature of dynamic operating conditions and the limitations inherent in short-term forecasting techniques, which collectively pose significant challenges to achieving reliable predictions. To enhance the accuracy of PEMFC degradation forecasting, this research proposes an integrated approach that combines the complete ensemble empirical mode decomposition with the variational mode decomposition (CEEMD-VMD) and triple echo state network (TriESN) to predict the deterioration process precisely.

On-Site Serine Delivery Drives Fermentation Pathway Reprogramming to Reverse Intracellular Persistence.

Intracellular bacteria that survive high-dose antibiotic treatment are recognized as persisters, serving as reservoirs for recurrent infections. While enhancing bacterial metabolism has restored antibiotic efficacy against planktonic persisters, it is ineffective against intracellular forms due to host-imposed nutrient deprivation. To address this, we developed FAlsBm@Rif, a poly(amino acid)-based nanodrug designed to sensitize intracellular persisters to antibiotics via on-site serine delivery.

An integrated electrochemiluminescence microfluidic device for detecting ultra-low concentration of ceftriaxone.

Ceftriaxone sodium, a widely used third-generation cephalosporin antibiotic, poses significant health risks when residues accumulate in clinical or environmental settings. However, conventional detection techniques, including high-performance liquid chromatography and capillary electrophoresis, are limited by complex procedures, poor sensitivity, and a lack of real-time capability. To address these challenges, we report a sensitivity-enhanced electrochemiluminescence (ECL) microfluidic device for the rapid and label-free detection of ultra-low concentrations of ceftriaxone.

Proteomics and lipidomics analysis of the breast muscles to investigate the effect of dietary copper level on the meat quality of pigeons.

Pigeon meat, known for its high nutritional value and tender texture, can be influenced by various dietary factors. Copper, an essential trace mineral, plays a vital role in growth, development, and overall meat quality. However, its impact on pigeon meat has not been well studied.

The Advancement of Prime Editing Technology.

The advent of CRISPR/Cas genome editing has spurred major breakthroughs across life sciences, offering vast potential across numerous research and application fields. Among the expanding toolkit of CRISPR/Cas-derived methods, prime editing (PE) stands out for its versatility and specificity, enabling precise point mutations and small insertions or deletions without requiring double-stranded DNA breaks. Since its introduction, PE has undergone multiple rounds of optimization to improve performance.

SLC7A11 is an unconventional H transporter in lysosomes.

Lysosomes maintain an acidic pH of 4.5-5.0, optimal for macromolecular degradation.

Degradation Prediction of PEMFCs Based on Discrete Wavelet Transform and Decoupled Echo State Network.

Predicting the degradation process of proton exchange membrane fuel cells (PEMFCs) under diverse operational conditions is crucial for their maintenance planning and health monitoring, but it is also quite complex. The variability in dynamic conditions and the shortcomings of short-term forecasting methods make accurate predictions difficult in practice. To strengthen the precision of deterioration predictive methods, this study introduces a degradation prediction of PEMFCs incorporating discrete wavelet transform (DWT) and a decoupled echo state network (DESN).

Mdga2 deficiency leads to an aberrant activation of BDNF/TrkB signaling that underlies autism-relevant synaptic and behavioral changes in mice.

Memprin/A5/mu (MAM) domain containing glycosylphosphatidylinositol anchor 2 (MDGA2) is an excitatory synaptic suppressor and its mutations have been associated with autism spectrum disorder (ASD). However, the detailed physiological function of MDGA2 and the mechanism underlying MDGA2 deficiency-caused ASD has yet to be elucidated. Herein, we not only confirm that Mdga2 +/- mice exhibit increased excitatory synapse transmission and ASD-like behaviors, but also identify aberrant brain-derived neurotrophic factor/tyrosine kinase B (BDNF/TrkB) signaling activation in these mice.

Emergence of a clinical carbapenem-resistant Escherichia coli co-harboring bla and mcr-1.1 on the same plasmid.

Objectives: Escherichia coli is a major pathogen, and the emergence of carbapenem-resistant E. coli (CREC) significantly restricts clinical treatment options. Polymyxins are considered the last-line treatment for CREC infections; however, the occurrence of polymyxin-resistant CREC, particularly following reports of plasmid-mediated colistin resistance (mcr), poses an increasing challenge.

Performance of ddPCR-GNB for microbial diagnosis of suspected bloodstream infection due to the four most common gram-negative bacteria: a prospective, multicenter study.

Unlabelled: We aimed to validate the performance of ddPCR-GNB, a plasma droplet digital PCR panel targeting the four most common gram-negative bacteria, for patients with suspected bloodstream infection (BSI). Patients suspected of having BSIs were prospectively enrolled. The results of blood culture and ddPCR-GNB were compared, and cases with discordant results were arbitrated on the basis of additional microbiological results and clinical evidence.

RPS23RG1 inhibits SORT1-mediated lysosomal degradation of MDGA2 to protect against autism.

Mutations in the synaptic protein MAM domain containing glycosylphosphatidylinositol anchor 2 (MDGA2) have been associated with autism spectrum disorder (ASD). Therefore, elucidating the regulatory mechanisms of MDGA2 can help develop effective treatments for ASD. Liquid chromatography-tandem mass spectrometry was carried out to identify proteins interacting with the extracellular domain of RPS23RG1 and with MDGA2, followed by co-immunoprecipitation assays to confirm protein-protein interactions.

Identification of mutation of MYOC (c.1099G>A), a pedigree pathogenic gene of juvenile open angle glaucoma (JOAG): A case report.

Rationale: The MYOC gene is associated with juvenile open-angle glaucoma (JOAG). This study aims to provide genetic counseling for a Chinese JOAG family by detecting MYOC mutations to identify high-risk individuals for early JOAG intervention. It also supplements the clinical characteristics of glaucoma patients with MYOC gene mutations.

Electrophysiology-based screening identifies neuronal HtrA serine peptidase 2 (HTRA2) as a synaptic plasticity regulator participating in tauopathy.

Long-term potentiation (LTP) and long-term depression (LTD) are widely used to study synaptic plasticity. However, whether proteins regulating LTP and LTD are altered in cognitive disorders and contribute to disease onset remains to be determined. Herein, we induced LTP and LTD in the hippocampal CA3-CA1 Schaffer collateral pathway, respectively, and then performed proteomic analysis of the CA1 region.

Antimicrobial Silicon Rubber Crosslinked with Bornyl-Siloxane.

Silicone rubber (SiR) has a wide range of medical applications, but it lacks antimicrobial properties, leading to potential infection issues with related implants or medical devices. Most studies focus on adding anti-bacterial agents or surface modification, which usually result in composites with anti-bacterial properties, rather than synthesizing SiR with intrinsically antimicrobial performances. To tackle this issue, a double substituted bornyl-siloxane crosslinker (BC) is designed.

Mismatch prime editing gRNA increased efficiency and reduced indels.

Prime editing enables precise and efficient genome editing, but its efficacy is hindered by pegRNA's 3' extension, forming secondary structures due to high complementarity with the protospacer. The continuous presence of the prime editing system also leads to unintended indel formation, raising safety concerns for therapeutic applications. To address these challenges, we develop a mismatched pegRNA (mpegRNA) strategy that introduces mismatched bases into the pegRNA protospacer, reducing complementarity and secondary structure formation, and preventing sustained activity.

[Development of a tumor organoid culture system with peptide-based hydrogels].

Peptide-based hydrogel, the polymer materials with a special network structure, are widely used in various fields of biomedicine due to their stable properties and biocompatibility. Environment-responsive self-assembled peptide aqueous solutions can respond to environment changes by the self-assembly of peptides into nanofiber networks. Peptide-based hydrogels well simulate the extracellular matrix and cell growth microenvironment, being suitable for 3D cell culture and organoid culture.

Fractional-order stochastic gradient descent method with momentum and energy for deep neural networks.

In this paper, a novel fractional-order stochastic gradient descent with momentum and energy (FOSGDME) approach is proposed. Specifically, to address the challenge of converging to a real extreme point encountered by the existing fractional gradient algorithms, a novel fractional-order stochastic gradient descent (FOSGD) method is presented by modifying the definition of the Caputo fractional-order derivative. A FOSGD with moment (FOSGDM) is established by incorporating momentum information to accelerate the convergence speed and accuracy further.

The presence of spontaneous echo contrast didn't increase the risk for left atrial appendage closure: A propensity score matching analysis based on the CLACBAC study.

Background: Left atrial appendage closure (LAAC) was effective in preventing thromboembolic events and stroke in patients with atrial fibrillation (AF). However, whether left atrial spontaneous echo contrast (LA-SEC) poses a higher risk for thromboembolism is contradictory. We aimed to investigate whether LA-SEC is a risk factor for thromboembolic events in patients who underwent LAAC.

GABA system in the prefrontal cortex involved in psychostimulant addiction.

Drug addiction is a chronic and relapse brain disorder. Psychostimulants such as cocaine and amphetamine are highly addictive drugs. Abuse drugs target various brain areas in the nervous system.