Immobilization of Cd Through Biosorption by C10-4 and Remediation of Cd-Contaminated Soil.

In this study, a highly cadmium (II)-resistant bacterium strain, C10-4, identified as , was isolated from a sediment sample collected from Baiyangdian Lake, China. The minimum inhibitory concentration (MIC) of Cd(II) for strain C10-4 was 1600 mg/L. Factors such as the contact time, pH, Cd(II) concentration, and biomass dosage affected the adsorption of Cd(II) by strain C10-4.

Barthel Index Score at Admission to Predict 1-Month and 1-Year Prognosis in Inpatients Aged ≥ 75 Years With Multimorbidity.

Objective: To investigate the Barthel Index (BI) score in predicting the 1-month and 1-year prognosis after discharge.

Methods: This was a retrospective observational single-center study. We retrospectively enrolled consecutive inpatients aged ≥ 75 years from a large public hospital.

Deletion of SH2D5 alleviates epileptic seizures and NMDAR expression via autophagic degradation of STAT1.

Epilepsy is a common neurological disorder resulting from an imbalance between neuronal excitation and inhibition. Synapses play a pivotal role in the pathogenesis of epilepsy. Src-homology 2 (SH2) domain-containing protein 5 (SH2D5) is highly expressed in the brain and is implicated in the regulation of synaptic function.

Underappreciated role of canopy nitrogen deposition for forest productivity.

Atmospheric nitrogen (N) deposition is generally expected to stimulate plant carbon (C) sequestration and promote tree growth, thereby mitigating atmospheric CO accumulation. Yet, the magnitude of N deposition contribution to forest productivity remains contentious. While correlative studies suggest substantial plant growth enhancement, controlled fertilization experiments typically demonstrate a limited impact.

Controlling the movement of nanoparticles in a dielectric metasurface by using rotatable linearly polarized light.

We propose a nanoparticle transportation method in a dielectric metasurface for nanoparticles of particular sizes using rotatable linearly polarized light. The dielectric metasurface utilizes plum-blossom structures to realize hot spot position switching by rotating the polarization state of incident light. The convex point of the plum-blossom dielectric metasurface always has a hot spot with higher intensity than that in the concave point, and there is an unbalanced potential well between the two adjacent plum-blossom dielectric metasurface units so that the nanoparticles are more easily attracted and transferred to the adjacent unit with a deeper potential well.

ZrMOF-based bimetallic nanotags enable dual-channel electrochemical biosensing for parallel and ultrasensitive detection of S. typhimurium and E. coli O157:H7.

Foodborne pathogens are a leading cause of food safety incidents. Here, we developed a highly sensitive dual-channel biosensor for the parallel electrochemical detection of Salmonella typhimurium (S. typhimurium) and Escherichia coli (E.

Design of mismatch closure for enhanced specificity in DNA strand displacement reactions.

Specific and sensitive DNA hybridization plays a key role in biotechnology, nanotechnology, and medical technology. However, traditional DNA hybridization-based strategies often require careful tuning of the binding affinity of the probe to attain a trade-off between specificity and sensitivity. Herein, we proposed energy barrier-gated dynamic selectivity to overcome this limitation.

Transcriptome- and Metabolome-Based Regulation of Growth, Development, and Bioactive Compounds in (Lamiaceae) Seedlings by Different Phosphorus Levels.

Phosphorus (P), as one of the essential bulk elements for plant growth and development, plays an important role in root growth, accumulation of secondary metabolites, and regulation of gene expression. In Bunge (), an important medicinal plant, the accumulation of its active components is closely related to the level of phosphorus supply, but the molecular regulatory mechanism of phosphorus treatment in the growth and secondary metabolism of is not clear. In this study, we investigated the effects of low phosphorus (P2), moderate phosphorus (P4), and high phosphorus (P6) treatment on the growth and development of .

Electrochemical detection of S. typhimurium based on peroxidase-like activity of gold nanoparticle-doped CuZr-MOF nanozyme.

An electrochemical biosensor using gold nanoparticles (AuNPs)-doped bimetallic-organic framework (BMOF) with enhanced peroxidase-like activity was constructed to detect Salmonella Typhimurium (S. typhimurium). The BMOF of CuZr-MOF was synthesized via a two-step method and used as carrier to in situ immobilize AuNPs.

Novel Light-Promoted Bioorthogonal Reaction via Molecular Recombination for the Synthesis of Polysubstituted Pyrrole and Its Application in In Vitro and In Vivo Studies.

Bioorthogonal chemistry has revolutionized organic, medicinal, and biochemical research by designing and developing selective and rapid reactions that do not interfere with biological processes. Despite significant advancements, the existing bioorthogonal reactions can normally introduce only one functional group to a biomolecule, limiting their versatility in biological systems. In this study, we report a novel light-promoting reaction, namely light-promoted bioorthogonal multifunctionalized molecular recombination (LBMR) reaction, that can facilitate the synthesis of polysubstituted pyrroles via molecular editing and recombination of isoxazole-3-carboxylate and isoxazole-3-carboxylic acid derivatives.

Preparation and Properties of Chitosan Complexes Consisting of Volatile Oil Nanoemulsion.

volatile oil (AAVO) is a kind of natural oil with abundant active components and remarkable medicinal and healthcare value. However, AAVO has low solubility, stability, and bioavailability. Here, to address these issues, a nanoemulsion system of volatile oil (AAVO-Ne) is constructed using phase transition titration, and the conditions are continuously optimized to combine it with chitosan, forming a chitosan composite of the volatile oil nanoemulsion (AAVO-NeCs).

Harnessing the FBXW7 Somatic Mutant R465C for Targeted Protein Degradation.

Targeted protein degradation (TPD) is a pharmacological strategy that eliminates specific proteins from cells by harnessing cellular proteolytic degradation machinery. In proteasome-dependent TPD, expanding the repertoire of E3 ligases compatible with this approach could enhance the applicability of this strategy across various biological contexts. In this study, we discovered that a somatic mutant of FBXW7, R465C, can be exploited by heterobifunctional compounds for targeted protein degradation.

Discovery of DCAF16 Binders for Targeted Protein Degradation.

Conventional small-molecule drugs primarily operate by inhibiting protein function, but this approach is limited when proteins lack well-defined ligand-binding pockets. Targeted protein degradation (TPD) offers an alternative approach by harnessing cellular degradation pathways to eliminate specific proteins. Recent studies have expanded the potential of TPD by identifying additional E3 ligases, with DCAF16 emerging as a promising candidate for facilitating protein degradation through both proteolysis-targeting chimera (PROTAC) and molecular glue mechanisms.

Systematic Analysis of UFMylation Family Genes in Tissues of Mice with Metabolic Dysfunction-Associated Steatotic Liver Disease.

Background/objectives: UFMylation, a newly identified ubiquitin-like modification, modulates a variety of physiological processes, including endoplasmic reticulum homeostasis maintenance, DNA damage response, embryonic development, and tumor progression. Recent reports showed that UFMylation plays a protective role in preventing liver steatosis and fibrosis, serving as a defender of liver homeostasis in the development of metabolic dysfunction-associated steatotic liver disease (MASLD). However, the regulation of UFMylation in MASLD remains unclear.

Advancing precision and personalized breast cancer treatment through multi-omics technologies.

Breast cancer is the most common malignant tumour in women, with more than 685,000 women dying of breast cancer each year. The heterogeneity of breast cancer complicates both treatment and diagnosis. Traditional methods based on histopathology and hormone receptor status are now no longer sufficient.

D2HGDH Deficiency Regulates Seizures through GSH/Prdx6/ROS-Mediated Excitatory Synaptic Activity.

Current antiepileptic drugs are ineffective in one-third of patients with epilepsy; however, identification of genes involved in epilepsy can enable a precision medicine approach. Here, it is demonstrated that downregulating D-2-hydroxyglutarate dehydrogenase (D2HGDH) enhances susceptibility to epilepsy. Furthermore, its potential involvement in the seizure network through synaptic function modulation is investigated.

Exploiting the DCAF16-SPIN4 interaction to identify DCAF16 ligands for PROTAC development.

Traditional small molecule drugs often target protein activity directly, but challenges arise when proteins lack suitable functional sites. An alternative approach is targeted protein degradation (TPD), which directs proteins to cellular machinery for proteolytic degradation. Recent studies have identified additional E3 ligases suitable for TPD, expanding the potential of this approach.

Harnessing the FBXW7 somatic mutant R465C for targeted protein degradation.

Targeted protein degradation (TPD) is a pharmacological strategy that eliminates specific proteins from cells by harnessing cellular proteolytic degradation machinery. In proteasome-dependent TPD, expanding the repertoire of E3 ligases compatible with this approach could enhance the applicability of this strategy across various biological contexts. In this study, we discovered that a somatic mutant of FBXW7, R465C, can be exploited by heterobifunctional compounds for targeted protein degradation.

A phage amplification-assisted SEA-CRISPR/Cas12a system for viable bacteria detection.

Rapid and accurate detection of viable bacteria is essential for the clinical diagnosis of urinary tract infections (UTIs) and for making effective therapeutic decisions. However, most current molecular diagnostic techniques are unable to differentiate between viable and non-viable bacteria. In this study, we introduce a novel isothermal platform that integrates strand exchange amplification (SEA) with the CRISPR/Cas12a system, thereby enhancing both the sensitivity and specificity of the assay and achieving detection of phage DNA at concentrations as low as 4 × 10 copies per μL.

TRPV4 drives the progression of leiomyosarcoma by promoting ECM1 generation and co-activating the FAK/PI3K/AKT/GSK3β pathway.

Purpose: Leiomyosarcoma (LMS) is an aggressive mesenchymal malignant tumor with poor therapeutic options, but the molecular mechanisms underlying LMS remain largely unknown. Increasing evidence indicates that transient receptor potential vanilloid 4 (TRPV4) levels are closely related to the advancement of various malignant tumors through diverse molecular mechanisms. However, the roles and regulatory mechanisms of TRPV4 in LMS progression remain unclear.

A platform for mapping reactive cysteines within the immunopeptidome.

The major histocompatibility complex class I antigen presentation pathways play pivotal roles in orchestrating immune responses. Recent studies have begun to explore the therapeutic potential of cysteines within the immunopeptidome, such as the use of covalent ligands to generate haptenated peptide neoepitopes for immunotherapy. In this work, we report a platform for mapping reactive cysteines on MHC-I-bound peptide antigens.

Establishment and characterization of a patient-derived metastatic extraskeletal Ewing sarcoma cell line ES-ZSS-1.

The methods available for treating metastatic Ewing sarcoma (ES) are inadequate; thus, innovative therapeutic approaches need to be developed. However, the lack of clinically relevant ES models has hindered the discovery of drugs for this disease. In this study, we established and characterized a patient-derived xenograft (PDX) cell line model, which was constructed using tumor tissue from a patient with metastatic extraskeletal ES.

Learning lightweight tea detector with reconstructed feature and dual distillation.

Currently, image recognition based on deep neural networks has become the mainstream direction of research; therefore, significant progress has been made in its application in the field of tea detection. Many deep models exhibit high recognition rates in tea leaves detection. However, deploying these models directly on tea-picking equipment in natural environments is impractical; the extremely high parameters and computational complexity of these models make it challenging to perform real-time tea leaves detection.

Simultaneous detection of CaMV35S and NOS using fluorescence sensors with dual-emission silver nanoclusters and catalytic hairpin amplification strategy.

A dual-emission fluorescent biosensing method was developed for simultaneous determination of CaMV35S and NOS in genetically modified (GM) plants. Two designed hairpin DNA (H1, H2) sequences were used as templates to synthesize H1-AgNCs (λ = 570 nm, λ = 625 nm) and H2-AgNCs (λ = 470 nm, λ = 555 nm). By using H1-AgNCs and H2-AgNCs as dual-signal tags, combined with signal amplification strategy of magnetic separation to reduce background signal and an enzyme-free catalytic hairpin assembly (CHA) signal amplification strategy, a novel multi-target fluorescent biosensor was fabricated to detect multiple targets based on FRET between signal tags (donors) and magnetic FeO modified graphene oxide (FeO@GO, acceptors).