Immobilization of Cd Through Biosorption by C10-4 and Remediation of Cd-Contaminated Soil.

In this study, a highly cadmium (II)-resistant bacterium strain, C10-4, identified as , was isolated from a sediment sample collected from Baiyangdian Lake, China. The minimum inhibitory concentration (MIC) of Cd(II) for strain C10-4 was 1600 mg/L. Factors such as the contact time, pH, Cd(II) concentration, and biomass dosage affected the adsorption of Cd(II) by strain C10-4.

JJ100 and LR alleviate liver injury in mice caused by continuous high-dose-alcohol exposure by protecting the intestinal barrier, rebuilding the gut microbiota and regulating AMPK and TLR4/NF-κB signaling pathways.

Alcohol consumption poses a major global public health challenge, with excessive drinkers showing heightened susceptibility to alcoholic liver disease (ALD) - a condition with complex pathogenesis. In this study, we used male BALB/c mice with identical enterotypes to establish a sustained high-dose alcohol-induced liver injury model. The model was created through daily intragastric administration of 52% (v/v) ethanol (10 mL kg) for four consecutive weeks, allowing us to investigate the hepatoprotective effects of probiotic supplementation with and .

Random mutagenesis and semi-rational design enhance the tolerance of Metabacillus litoralis C44 α-L-rhamnosidase.

Isoquercitrin is a flavonoid with wide range of physiological activities, which has significant applications in the pharmaceutical and nutraceutical industries, but faces challenges in industrial production due to its low yields and high production cost. Based on a comparison of homologous protein sequences, D222 and E486 were identified as active sites of the α-L-rhamnosidase MlRha4 derived from Metabacillus litoralis C44. Random mutagenesis of the α-L-rhamnosidase MlRha4 gene sequence from strain C44 was performed using error-prone PCR, and reverse mutation was performed on the inactive mutants, followed by semi-rational design, resulting in 11 positive mutants.

Diversity and Functionality of Bacteria Associated with Different Tissues of Spider Heteropoda venatoria Revealed through Integration of High-Throughput Sequencing and Culturomics Approaches.

Spiders host a diverse range of bacteria in their guts and other tissues, which have been found to play a significant role in their fitness. This study aimed to investigate the community diversity and functional characteristics of spider-associated bacteria in four tissues of Heteropoda venatoria using HTS of the 16S rRNA gene and culturomics technologies, as well as the functional verification of the isolated strains. The results of HTS showed that the spider-associated bacteria in different tissues belonged to 34 phyla, 72 classes, 170 orders, 277 families, and 458 genera.

iTRAQ-based proteomic analysis of Deinococcus radiodurans in response to C heavy ion irradiation.

Background: Deinococcus radiodurans (D. radiodurans) is best known for its extreme resistance to diverse environmental stress factors, including ionizing radiation (IR), ultraviolet (UV) irradiation, oxidative stress, and high temperatures. Robust DNA repair system and antioxidant system have been demonstrated to contribute to extreme resistance in D.

Mirror proteases of Ac-Trypsin and Ac-LysargiNase precisely improve novel event identifications in MC 155 by proteogenomic analysis.

Accurate identification of novel peptides remains challenging because of the lack of evaluation criteria in large-scale proteogenomic studies. Mirror proteases of trypsin and lysargiNase can generate complementary / ion series, providing the opportunity to efficiently assess authentic novel peptides in experiments other than filter potential targets by different false discovery rates (FDRs) ranking. In this study, a pair of in-house developed acetylated mirror proteases, Ac-Trypsin and Ac-LysargiNase, were used in MC 155 for proteogenomic analysis.

Quantitative phosphoproteomics reveal cellular responses from caffeine, coumarin and quercetin in treated HepG2 cells.

Protein phosphorylation is the most common type of post-translational modification where serine, threonine or tyrosine are reversibly bound to the phosphate group of ATP in a reaction catalyzed by protein kinases. Phosphorylation plays an important role in regulation of cell homeostasis, including but not limited to signal perception and transduction, gene expression and function of proteins. Protein phosphorylation happens on a fast time scale and represents an energy-efficient way for the cell to adapt to exposure to chemical stressors.

Ac-LysargiNase efficiently helps genome reannotation of Mycolicibacterium smegmatis MC 155.

Accurate genome annotation, the foundation of life science research in the genome era, is hampered by limited known gene models, nonstandard start codons, and the limited homology of annotated genes in other organisms. LysargiNase mirrors trypsin at the cleavage sites, providing the opportunity to identify peptides other than tryptic peptides. In this study, we used an in-house developed acetylated LysargiNase (Ac-LysargiNase) with higher activity and stability in non-pathogenic Mycolicibacterium smegmatis MC 155 to supplement the widely used trypsin in proteomic studies.

Cyclic Tetrapeptides with Synergistic Antifungal Activity from the Fungus Using LC-MS/MS-Based Molecular Networking.

Fungal natural products play a prominent role in the development of pharmaceuticalagents. Two new cyclic tetrapeptides (CTPs), westertide A () and B (), with eight known compounds (-) were isolated from the fungus guided by OSMAC (one strain-many compounds) and molecular networking strategies. The structures of new compounds were unambiguously determined by a combination of NMR and mass data analysis, and chemical methods.

Deep N-terminomics of Mycobacterium tuberculosis H37Rv extensively correct annotated encoding genes.

Mycobacterium tuberculosis (MTB) is a severe causing agent of tuberculosis (TB). Although H37Rv, the type strain of M. tuberculosis was sequenced in 1998, annotation errors of encoding genes have been frequently reported in hundreds of papers.

Analysis of the relationship between bile duct and duodenal microbiota reveals that potential dysbacteriosis is the main cause of primary common bile duct stones.

Bacteria play an important role in the formation of primary Common Bile Duct (CBD) stones. However, the composition and function of the microbiota of bile duct in patients with primary CBD stones remained to be explored. We utilized the 16S rRNA gene high-throughput sequencing technology to analyze the microbial diversity and community composition of biliary and duodenal microbiota in 15 patients with primary CBD stones and 4 patients without biliary tract diseases.

sp. nov., isolated from roots.

A Gram-stain-variable, aerobic, rod-shaped, endospore-forming strain R196) was isolated from internal tissues of roots of . Cells were motile with peritrichous flagella. The colonies were light pink on tryptone soya agar medium.

Discovery of Brominated Alboflavusins With Anti-MRSA Activities.

As methicillin-resistant (MRSA) is becoming a serious pathogenic threaten to human health worldwide, there is an urgent need to discover new antibiotics for the treatment of MRSA infections. Alboflavusins (AFNs) are a group of halogenated cyclohexapeptides with anti-MRSA activities. In this study, two novel brominated AFN congeners (compounds and ) were isolated from the wild-type strain sp.

Temporal Characteristics of the Oropharyngeal and Nasal Microbiota Structure in Crewmembers Stayed 180 Days in the Controlled Ecological Life Support System.

Confined experiments are carried out to simulate the closed environment of space capsule on the ground. The Chinese Controlled Ecological Life Support System (CELSS) is designed including a closed-loop system supporting 4 healthy volunteers surviving for 180 days, and we aim to reveal the temporal characteristics of the oropharyngeal and nasal microbiota structure in crewmembers stayed 180 days in the CELSS, so as to accumulate the information about microbiota balance associated with respiratory health for estimating health risk in future spaceflight. We investigated the distribution of microorganisms and their dynamic characteristics in the nasal cavity and oropharynx of occupants with prolonged confinement.

Open-pFind Enhances the Identification of Missing Proteins from Human Testis Tissue.

In recent years, high-throughput technologies have contributed to the development of a more precise picture of the human proteome. However, 2129 proteins remain listed as missing proteins (MPs) in the newest neXtProt release (2019-02). The main reasons for MPs are a low abundance, a low molecular weight, unexpected modifications, membrane characteristics, and so on.

High-coverage proteomics reveals methionine auxotrophy in Deinococcus radiodurans.

Deinococcus radiodurans is a robust bacterium best known for its capacity to resist to radiation. In this study, the SDS-PAGE coupled with high-precision LC-MS/MS was used to study the D. radiodurans proteome.

Draft Genome Sequence of Bacillus litoralis C44, Isolated from Chinese Scholar Tree (Sophora japonica) Forest Soil.

Bacillus litoralis C44 can hydrolyze rutin to produce isoquercetin by the enzyme α-l-rhamnosidase. We report here the genome sequence and annotation result of strain C44. The genomic information will serve as references to the physiology, genetics, and evolution of this species and further genetic engineering research in this species.