Chronic Exposure to Sunset Yellow Promotes Susceptibility to Experimental Colitis in Mice through Gut Microbiota.

Sunset yellow (SY) is a widely used food additive. However, its impacts on ulcerative colitis (UC) development remain unclear. Here, SY exposure exacerbated dextran sulfate sodium (DSS)-induced UC symptoms in mice, including body weight loss, elevated disease activity index, histological damage, inflammation, gut barrier impairment, disruption of gut microbiota composition, and sulfur metabolism.

The cation channel Trpa1 and chemokine Cxcl1 mediate axonal degeneration in spared nerve injury-induced neuropathic pain.

Demyelination of peripheral nerve injury is a vital cause of neuropathic pain. Schwann cells play an important role in supporting and maintaining the removal and regeneration of myelin debris from neuronal axons in the peripheral nervous system. Creating a good immune microenvironment would promote the Schwann cells to repair the injured nerve and reverse the allodynia of neuropathic pain.

Primary Sequence-Intrinsic Immune Evasion by Viral Proteins Guides CTL-Based Vaccine Strategies.

Viruses use a range of sophisticated strategies to evade detection by cytotoxic T-lymphocytes (CTLs) within host cells. Beyond elaborating dedicated viral proteins that disrupt the MHC class I antigen-presentation machinery, some viruses possess intrinsic, cis-acting genome-encoded elements that interfere with antigen processing and display. These protein features, including G-quadruplex motifs, repetitive peptide sequences, and rare-codon usage, counterintuitively limit production of proteins critical to virus survival, particularly during latency.

The expression trend and regulatory role of miR-484 in patients with gestational diabetes mellitus.

Objective: Gestational diabetes mellitus (GDM) is characterized by the onset of abnormal glucose metabolism during pregnancy. This study aims to examine the expression patterns and functional significance of miR-484 in GDM.

Methods: The levels of miR-484 in GDM patients were compared to those in normal pregnant women through RT-qPCR.

Long-Read Sequencing Reveals Tumor-Specific Splicing Isoforms as Therapeutic Targets In NSCLC.

Despite extensive transcriptomic alterations observed in tumors, the global landscape of isoform-level alternative splicing in cancer remains largely unexplored. Leveraging long-read sequencing, we successfully identified and characterized full-length isoforms, along with tumor-specific splicing events in non-small cell lung cancer (NSCLC). Our analysis identified 38,058 previously unannotated isoforms, which were subsequently validated using orthogonal multi-omics datasets to confirm their transcriptional and translational activities.

Spatial Organization of the Metabolic Pathway for Enhancing l-Fucose Biosynthesis in Engineered .

Synthetic biology and metabolic engineering have revolutionized the microbial biosynthesis of high-value compounds, yet conventional strategies are frequently confronted with metabolic burden and flux competition. Inspired by natural systems that employ spatial organization to enhance catalytic efficiency, we have developed advanced techniques to optimize pathway performance in engineered microbes. In this study, we present a systematic implementation of spatial organization strategies─consisting of scaffold-free enzyme assembly mediated by interacting peptides and engineered protein compartments─to improve l-fucose biosynthesis in .

Effects of an inflatable lumbar pillow on phenylephrine dose for spinal hypotension in cesarean delivery: an unblinded randomized controlled trial.

Background: Aortocaval compression by the gravid uterus in the supine position may exacerbate spinal hypotension during cesarean delivery. We developed an inflatable lumbar pillow (ILP) to elevate the uterus in the supine position. The aim of this study was to evaluate the effect of the ILP on the dose of phenylephrine to prevent hypotension after spinal anesthesia in cesarean delivery.

LXR-β regulates microglial efferocytosis and neuroinflammation in CPSP via STAT6 activation.

Background: Central post-stroke pain (CPSP) is a chronic neuropathic pain syndrome that develops following cerebrovascular injury and currently lacks effective treatment options. Previous research from our group has found a significant number of apoptotic cells in the thalamus of CPSP rats, and in the nervous system, the failure to promptly clear apoptotic cell debris can activate microglia, triggering a persistent neuroinflammatory response that contributes to the onset and progression of CPSP. Microglia clear apoptotic cells in the central nervous system through efferocytosis, a process that reduces neuroinflammation and promotes the reprogramming of microglia toward the M2 phenotype, which is crucial for immune defense and repair mechanisms in the central nervous system.

From information exposure to legal consciousness: The chain mediating role of political trust and social equality in Chinese college students.

The cultivation of college students' legal consciousness in the digital media environment is crucial for advancing social responsibility, moral awareness, and rule-of-law societies. However, prior research often assumes a linear positive relationship between information exposure and legal consciousness, overlooking potential negative effects in which algorithmic biases and emotional framing in digital media may weaken legal consciousness. Existing studies rarely explore the chain mediation of political trust and social equality perceptions, particularly lacking empirical tests on Chinese college students.

Hesperetin Protects from Palmitic-Acid-Induced Lipotoxicity through the Inhibition of Glutaminolysis, mTORC1 Signaling, and Limited Apoptosis.

Palmitic acid-induced lipotoxicity contributes to the development of nonalcoholic fatty liver disease (NAFLD). Hesperetin has been reported to alleviate oxidative stress, inflammation, and cell death in NAFLD, while its potential to mitigate palmitic acid-induced lipotoxicity remains unexplored. This study investigates the protective effects of hesperetin on palmitic-acid-stimulated lipotoxicity and elucidates the underlying molecular mechanisms.

Perpendicular Magnetic Anisotropy in FeRh Thin Films with Coexisting Magnetic Phases.

The advantages of high integration density, energy efficiency, and enhanced stability make the realization of perpendicular magnetic anisotropy (PMA) in single-layer films a crucial step toward the development of advanced spintronic devices. A theoretical study predicted that the magnetic easy axis of FeRh film would reorient from out-of-plane (OP) to in-plane (IP) directions during the antiferromagnetic-ferromagnetic (AF-FM) phase transition. However, few studies have observed an OP magnetic anisotropy in FeRh films.

L1, a 3,3'-diindolylmethane-derivative, induced ER stress-mediated apoptosis and suppressed growth through the FLI1/AKT pathway in erythroleukemia HEL cells.

Introduction: 3,3'-Diindolylmethane (DIM) is a major phytochemical product derived from ingestion of cruciferous vegetables. As an effective cancer chemopreventive agent, DIM has been used in preclinical and clinical trials. Recently, our group synthesized and modified a novel DIM derivative, L1, and demonstrated its significant antileukemic activities.

Design, synthesis and SARS-CoV‑2 main protease inhibitory activities of 2-arylthiomethyl-6-bromoindole derivatives.

COVID-19, caused by SARS-CoV-2, has led to a global health crisis. The main protease (M) is essential for viral replication, making it a promising target for the development of anti-COVID-19 therapeutics. In this paper, series of novel 2-arylsulfanylmethyl-6-bromoindole derivatives (I, II III and IV) were designed as 2019-nCoV main protease inhibitors.

Evaluation and genome-wide association study of saline-alkali tolerance in high-latitude rice resource populations.

Introduction: China is the world's third largest saline-alkali land country, and the breeding of salt-tolerant rice varieties has always been a key focus of rice breeders. Screening and identifying salt-tolerant varieties and exploring related genes are essential for breeding.

Methods: In this study, 450 high-latitude resource populations were planted on natural saline-alkali soil for 2 years under 2 treatments.

Spectral calibration method of the field spectrometer using combined solar Fraunhofer lines and atmospheric absorption characteristics: case study at the Baotou RadCalNet site and implications for reflectance measurement.

Accurate spectral parameters are fundamental for the quantitative application of hyperspectral data, particularly in the automated measurement of surface characteristic parameters. With the widespread use of networked ground spectrometers, the performance of these instruments is significantly influenced by environmental conditions, making the accuracy of spectral parameters crucial for reliable radiometric measurements. In this study, we employed a combination of atmospheric absorption features and solar Fraunhofer lines to calibrate and evaluate the spectral parameters of the spectrometer, applying it to an automated measurement spectrometer at the Baotou site, one of the Radiometric Calibration Network (RadCalNet) sites.

IPD regimen effect on the levels of VEGF and IL-6 in elderly patients with recurrent multiple myeloma.

Background: To investigate IPD regimen effect on vascular endothelial growth factor (VEGF) and interleukin-6 (IL-6) levels in elderly patients with recurrent multiple myeloma (MM).

Methods: Eighty-two elderly patients with relapsed multiple myeloma in our hospital from January 2019 to December 2021 were selected and randomly divided into TD group and IPD group, 41 cases in each group. The TD group was treated with thalidomide + dexamethasone, while the IPD group was treated with ixazomib + pomalidomide + dexamethasone.

TriAGe+ Score and NSE for Detecting Acute Ischemic Stroke in Acute Isolated Dizziness: A Propensity Score Matching Analysis.

Objectives: Acute isolated dizziness often presents diagnostic challenges, as peripheral vestibular disorders (PVD) and acute ischemic stroke (AIS) symptoms overlap. This study aimed to evaluate the diagnostic effectiveness of the TriAGe+ score, serum neuron-specific enolase (NSE), and their combination in differentiating AIS from PVD.

Methods: A retrospective analysis was conducted on patients hospitalized for acute isolated dizziness in our hospital.

3-O-Acetyl-11-Keto--Boswellic Acid Suppresses Colitis-Associated Colorectal Cancer by Inhibiting the NF-Kb Signaling Pathway and Remodeling Gut Microbiota.

Objectives: Colorectal cancer (CRC) is one of the most common cancers all over the world. The progression of CRC is associated with inflammation and disruptions in intestinal flora. 3-O-Acetyl-11-keto-β-boswellic acid (AKBA) has been noted for its potent anti-inflammatory properties.

Inhibition of UBE2C Promotes Parkin-Mediated K63-Linked Ubiquitination of TOP2A to Induce Senescence and Increase Sensitivity of Doxorubicin in Breast Cancer.

Breast cancer is now the second most commonly diagnosed cancer and the most common female malignancy. Chemotherapy-based adjuvant therapy after surgery and neoadjuvant therapy before surgery are cornerstones of breast cancer treatment. Doxorubicin is one of the most commonly used anthracycline chemotherapy treatments for breast cancer; however, doxorubicin resistance is a major barrier to its clinical use.

CD63 Immunological Activation Versus Hemostatic Function: Platelet Alterations After Polytrauma.

Platelets are attributed an increasing role in the post-traumatic immune response. The exact mechanisms, particularly the link between immune response and hemostasis, have not been conclusively established. This study aimed to investigate the activity marker CD63 on platelets after polytrauma and its significance for hemostasis.

Maresin1 Alleviates Ischemia Reperfusion Injury After Lung Transplantation by Inhibiting Ferroptosis via the PKA-Hippo-YAP Signaling Pathway.

: Lung ischemia reperfusion injury (LIRI) is a severe complication after lung transplantation (LT). Ferroptosis contributes to the pathogenesis of LIRI. Maresin1 (MaR1) is an endogenous pro-resolving lipid mediator that exerts protective effects against multiorgan diseases.

Facile synthesis and biological evaluation of [a]-benzannulated BODIPY derivatives as novel heavy-atom-free photosensitizers for photodynamic anticancer therapy.

Boron dipyrromethenes (BODIPYs) have garnered significant attention for their broad applications in photodynamic anticancer therapy. However, the development of these materials remains a challenging task, partially due to their low singlet oxygen (O) quantum yield usually require incorporating heavy atoms and high laser power density to produce thermal energy and synergistically enhance photodynamic therapy (PDT). Concerns about potential dark toxicity of heavy atoms and the strong photothermal radiation causing acute pain significantly reduces patient compliance, hinders the broader clinical application of PDT.

Beyond Regional Boundaries: Insights From ICU Nursing Practices in Western China to Advance Global Strategies Patient Experience Strategies.

Background: As front-line caregivers in high-risk settings, intensive care unit (ICU) nurses worldwide play a pivotal role in redefining patient-centered critical care. Their daily practices not only influence individual patient outcomes and experiences but also provide transferable insights that can inform evidence-based and humanistic models of care. By examining how ICU nurses improve patient experiences-through structured communication frameworks, family engagement protocols, and innovative approaches to pain management-we can integrate these strategies into clinical workflows, professional education, and policy development.

Immunosuppressive mechanisms and therapeutic targeting of regulatory T cells in ovarian cancer.

Ovarian cancer remains the most lethal gynecologic malignancy, largely due to its late-stage diagnosis and immunosuppressive tumor microenvironment (TME). A key mediator of immune evasion in ovarian cancer is the infiltration and activation of regulatory T cells (Tregs), which suppress antitumor immunity and foster therapeutic resistance. Emerging therapeutic strategies to target Tregs-such as cytokine modulation, checkpoint blockade, metabolic inhibitors, and epigenetic regulators-are critically evaluated for their potential to restore antitumor immunity.

The Decrease of Soil Microbial Community Diversity and Network Complexity Results in the Increase of Soil-Borne Diseases With Monocultural Years in Greenhouse Tomato Production Systems.

The excessive use of water and fertiliser, along with long-term monocultivation in greenhouse vegetable fields, has led to a decrease in soil quality and an imbalance in the soil microflora, which may contribute to worsening soil-borne diseases. To examine the associations between the soil microbial community composition and disease-suppressing microorganisms under different years of cultivation shifting from croplands to greenhouses, we collected soil samples from fields continuously planted with tomatoes for 5, 10 and 20 years, as well as from nearby maize-wheat fields (MW). The soil physicochemical properties, microbial community composition, putative plant pathogens and putative antagonistic bacteria were analysed.