[Synthesis of a temperature-responsive multimodal motion microrobot capable of precise navigation for targeted controllable drug release].

Objectives: To synthesize a temperature-responsive multimodal motion microrobot (MMMR) using temperature and magnetic field-assisted microfluidic droplet technology to achieve targeted drug delivery and controlled drug release.

Methods: Microfluidic droplet technology was utilized to synthesize the MMMR by mixing gelatin with magnetic microparticles. The microrobot possessed a magnetic anisotropy structure to allow its navigation and targeted drug release by controlling the temperature field and magnetic field.

In vivo self-assembled siRNAs ameliorate neurological pathology in TDP-43-associated neurodegenerative disease.

Abnormal accumulation of TAR DNA-binding protein-43 (TDP-43) is a hallmark of amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Small interfering RNAs (siRNAs) targeting TDP-43 offer potential therapeutic strategies for these diseases. However, efficient and safe delivery of siRNAs to the central nervous system (CNS) remains a critical challenge.

Regulation of food intake by Connexin43 via adipocyte-sensory neuron electrical synapses.

Background And Objective: Connexin43 (Cx43), encoded by Gja1, forms gap junctions between adjacent cells. In adipose tissue, it is upregulated during adipose beiging while downregulated by high-fat-diet (HFD) feeding. Adipocyte-specific Gja1 overexpression enhances adipose tissue beiging in response to mild cold stress of room temperature.

Discovery of Potential GPRC5D Inhibitors through Virtual Screening and Molecular Dynamics Simulations.

G protein-coupled receptor family C, group 5, member D (GPRC5D), a member of the G protein-coupled receptor (GPCR) family, has recently emerged as a promising target for immunotherapy in hematologic malignancies, particularly multiple myeloma. However, no systematic virtual screening studies have been conducted to identify small-molecule inhibitors targeting GPRC5D. To address this gap, a multistep computational screening strategy is developed that integrates Protein-Ligand Affinity prediction NETwork (PLANET), a GPU-accelerated version of AutoDock Vina (Vina-GPU), molecular mechanics/generalized born surface area (MM/GBSA), and an online tool for Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) property prediction (admetSAR 3.

MOF-templated hollow Pd/CdS@CoS nanocages with synergistic Z-scheme/Schottky effects for photoelectrochemical biosensing of chlorpyrifos featuring exceptional dynamic range.

A novel ternary synergistic photoelectrochemical (PEC) probe is presented utilizing metal-organic framework (MOF)-templated Pd/CdS@CoS nanocages for sensing chlorpyrifos (CPF) using chronoamperometry under an applied bias of - 65 mV with 465-nm LED illumination. Derived from ZIF-67 via in situ sulfidation, the hollow nanocage architecture integrated CdS nanoparticles with CoS to form a direct Z-scheme heterojunction, while decorating Pd quantum dots (QDs) created a Schottky barrier, implementing a crucial dual charge-transfer enhancement strategy. Density functional theory (DFT) simulations confirmed a 0.

G Protein-coupled receptors: key targets for maintaining the function of basal ganglia-thalamus-cortical circuits in Parkinson's disease.

Parkinson's Disease (PD), the second most common neurodegenerative disease after Alzheimer's disease, is clinically characterized by resting tremor, rigidity and postural balance disorder. Its pathological essence is the progressive degenerative death of dopaminergic neurons in the substantia nigra pars compacta (SNpc), leading to a significant decrease in striatal dopamine (DA) levels. This results in the dysfunction of basal ganglia-thalamus-cortex (BGTC) circuit.

Proteomics-based study for evaluation crossbreeding advantages of cattle-yak milk.

Cattle-yaks are increasingly cultivated to boost yak dairy production while reports claim that will decrease the nutritional value of yak milk. However, studies on protein composition differences, especially functional proteins, between yak and cattle-yak milk are limited. This study explores these differences using data-independent acquisition (DIA) proteomics to compare whey proteins and milk fat globule membrane (MFGM) proteins in both types of milk.

DDX55 safeguards naïve T cell homeostasis by suppressing activation-promoting transposable elements.

Naïve T cells are maintained in a homeostatic state to preserve a stable T cell pool with diverse T cell receptor (TCR) repertoires, ensuring preparedness for priming. However, the underlying mechanisms controlling naïve T cell homeostasis and priming remain unclear. Leveraging a machine learning-based functional genetic screen, we identified () as the top factor responsible for naïve T cell homeostasis.

Machine learning- and multilayer molecular network-assisted screening hunts fentanyl compounds.

Fentanyl and its analogs are a global concern, making their accurate identification essential for public health. Here, we introduce Fentanyl-Hunter, a screening platform that uses a machine learning classifier and multilayer molecular network to select and annotate fentanyl compounds using mass spectrometry (MS). Our classification model, based on 772 fentanyl spectra and spectral binning feature engineering, achieved an score of 0.

Genome-wide identification of in and the role of in rubber biosynthesis.

Oliv., a Tertiary period relict tree species endemic to China, is a rubber-producing plant valued for both medicinal and edible applications. rubber is a high-quality natural rubber prized for its excellent elasticity, abrasion resistance, and insulation properties, leading to broad industrial applications.

Identification of kidney renal clear cell carcinoma prognosis based on gene expression and clinical information.

Background: Kidney renal clear cell carcinoma (KIRC) prognosis exhibits substantial heterogeneity even among patients with identical clinicopathological staging, reflecting the limitations of current classification systems. Therefore, the development of reliable prognostic tools may improve clinical evaluation of KIRC outcomes and facilitate personalized therapy optimization.

Methods: The KIRC data of GSE40435 and GSE46699 in the GEO database were immunologically grouped based on 29 immune gene sets through R language.

Co-Targeting BCL-xL with MCL-1 Induces Lethal Mitochondrial Dysfunction in Diffuse Mesothelioma.

Diffuse mesothelioma is a rare but highly aggressive and treatment-resistant neoplasm with low survival rates. Effective therapeutic strategies are limited, and resistance to treatment is a major obstacle. Myeloid cell leukemia (MCL)-1 and B-cell leukemia (BCL)-xL are antiapoptotic B-cell lymphoma 2 (Bcl-2) family proteins that block cell-intrinsic apoptosis through interactions on the mitochondrial outer membrane which contribute to therapeutic resistance.

SLC38A1 protects against aging-related oxidative stress and lipid peroxidation in C2C12 myoblasts: Implication of a ferroptosis-related regulator for skeletal muscle aging.

Ferroptosis has been implicated in skeletal muscle aging. Nevertheless, specific ferroptosis-related genes (FRGs) governing skeletal muscle aging remain unclear. The aim of this study was to identify ferroptosis-related marker genes associated with skeletal muscle aging, uncovering potential therapeutic targets for skeletal muscle aging.

N-Doping/KOH Synergy in Waste Moss Biochar for Geosmin Removal in Aquaculture Water: Elucidating Surface Functionalization and Activation Mechanisms.

Taste and odor (T&O) has always been a critical issue in drinking water treatment and quality improvement of aquatic products [...

Chromatin Accessibility Dynamics Reveal Conserved Transcriptional Regulatory Networks During Insect Metamorphosis in and .

Insect metamorphosis is a complex developmental process regulated by hormonal signaling and gene transcription. To elucidate its transcriptional regulatory mechanisms, we examined chromatin accessibility dynamics during metamorphosis in two holometabolous insects, and , using ATAC-seq. Our analysis revealed distinct stage-specific chromatin accessibility patterns, with peak accessibility during the prepupal stage in and the wandering larval to prepupal transition in .

Microenvironment-driven transformable self-assembly nanoplatform enables spatiotemporal remodeling for rheumatoid arthritis therapy.

The aberrant vasculature within the inflamed joint cavity of rheumatoid arthritis (RA) not only exacerbates joint pathology but also restricts the effective delivery of therapeutic drugs. Herein, we propose a strategy that involves the rapid and sustained vasculature repair alongside microenvironment-driven drug delivery to achieve multifaceted RA management. The transformable, self-assembling nanoplatform specifically accumulates in the inflamed joint cavity guided by a vascular targeting peptide (STP).

Identification and Characterization of Glycine- and Proline-Rich Antioxidant Peptides From Antler Residues Based on Peptidomics, Machine Learning, and Molecular Docking.

The purpose of this study was to determine the optimal enzymatic hydrolysis process for antler residues and to identify and characterize the antioxidant peptides present in the products of this hydrolysis. The results indicated that when neutrase is employed as the hydrolase, with an enzyme concentration of 2300 U/g, a temperature of 45°C, a pH of 6.0, a hydrolysis duration of 7.

Research progress in glioma-related epilepsy (Review).

Epilepsy is a frequently occurring complication in patients with gliomas that substantially impairs their quality of life. The onset of epilepsy in patients with gliomas is driven by multiple mechanisms, including tumour-induced compression of the peripheral neural network, the release of neurotransmitters and inflammatory factors by tumour cells, alterations in the tumour microenvironment, and changes in gene expression. Collectively, these factors contribute to the abnormal excitability of local neurons, ultimately triggering seizures.

Exosomal miR-93-3p targets EIF4EBP1 to regulate macrophage polarization and accelerate wound healing post-anal fistula surgery.

Background: Delayed wound healing following anal fistula (AF) surgery remains a clinical challenge. This study endeavors to identify and validate key exosomal miRNAs that regulate postoperative inflammation after AF surgery by integrating multi-omics analyses with functional assays, and to elucidate the molecular mechanisms by which these miRNAs and their target genes influence macrophage M1/M2 polarization.

Methods: 15 patients undergoing AF surgery were randomized to three groups.

Associations of lactate-to-hematocrit ratio with short- and long-term prognoses in critically ill patients with cirrhosis and sepsis: a retrospective cohort study.

Background: The lactate-to-hematocrit ratio (LHR) is strongly associated with the prognosis of patients critically ill with various diseases. However, the relationship between LHR and the prognosis of critically ill patients with cirrhosis and sepsis has not been explored. Therefore, this study examined the association between the LHR and short- and long-term prognoses in this special group of patients.

Lipidomic profiling of human bile distinguishes cholangiocarcinoma from benign bile duct diseases with high specificity and sensitivity: a prospective descriptive study.

Background: Cholangiocarcinoma (CCA) is a rare and highly aggressive malignancy originating in the bile ducts. Owing to limitations involving pathological sampling, the clinical differentiation of CCA from benign biliary diseases remains challenging. This study aimed to evaluate the differences between the bile lipidomes of CCA patients and those of patients with benign disease to develop a bile lipid classifier that can help to differentiate CCA from benign conditions.

Oral virome metagenomic catalog links Porphyromonas gingivalis phages to obesity and type 2 diabetes.

The human microbiota has a critical role in maintaining human microbiome homeostasis and health, yet the viral component of the oral microbiome remains largely unidentified. We establish the Human Oral Virome Database (HOVD) catalog, a freely accessible online resource cataloging 24,440 bacteriophage viral operational taxonomic units and 83 eukaryotic viral genomes. Utilizing HOVD, we investigate oral virome variation and its correlation with oral bacteria and gut virome in 220 obese individuals with or without type 2 diabetes mellitus (T2D).

Au(III) Schiff base complexes as oxidoreductase inhibitors against carbapenem- and colistin-resistant Gram-negative bacteria via targeting redox active motifs.

Carbapenem- and colistin-resistant Gram-negative bacteria have become one of the most severe public health issues worldwide. The development of advanced antibacterial agents that can outpace microbial adaptation is imperative. The thioredoxin (Trx) and glutaredoxin (Grx) systems play important roles in maintaining redox homeostasis within Gram-negative bacterial cell membranes, with thioredoxin reductase (TrxR) and glutathione reductase (GR) being classical antibacterial targets.

Risk factors of early childhood caries among preschool children in Shanghai, China: a longitudinal study.

Introduction: Early childhood caries(ECC) has caused a growing public health burden worldwide, but there still remains a gap in the understanding of ECC in Shanghai, China. This study aims to investigate the current profile of ECC and related risk factors of new-onset caries among preschool children in Shanghai, China.

Materials And Methods: Children aged 3-5 were included in this two-year longitudinal study from Shanghai.