An In Vitro Tribological Astringency Assessment Method Based on Astringency Perception Mechanism.

Astringency is a complex oral sensation characterized by dryness and constriction in the mouth. It is typically induced by polyphenol-rich foods and beverages such as wine and tea. The quantitative assessment of astringency intensity has become a prominent research focus in the food science field.

Identification of kidney renal clear cell carcinoma prognosis based on gene expression and clinical information.

Background: Kidney renal clear cell carcinoma (KIRC) prognosis exhibits substantial heterogeneity even among patients with identical clinicopathological staging, reflecting the limitations of current classification systems. Therefore, the development of reliable prognostic tools may improve clinical evaluation of KIRC outcomes and facilitate personalized therapy optimization.

Methods: The KIRC data of GSE40435 and GSE46699 in the GEO database were immunologically grouped based on 29 immune gene sets through R language.

triggers type I interferon signaling to promote host defense against infection via mTORC1 activation.

Multidrug-resistant , a prevalent opportunistic pathogen in hospitals, presents a substantial risk to immunocompromised patients, triggering life-threatening conditions including pneumonia, bacteremia, and sepsis, which can be fatal. Therefore, it is urgent to develop new therapeutic strategies to combat and eradicate multidrug-resistant infection. Our finding reveals that promotes the mice treated with 5-fluorouracil (5-FU) to defend against multidrug-resistant infection via the mTORC1-IFN-I signaling pathway.

Comparative Omics Analysis of Four Grape Varieties and Exploration of Their Anthocyanin Synthesis Mechanisms.

: L. exhibits diverse varietal traits influencing fruit quality and stress tolerance. The summer black grape (Xiahei), known for its superior tolerance to abiotic stress and intense pigmentation, was hypothesized to possess distinct metabolic and genetic profiles, particularly in flavonoid and anthocyanin biosynthesis.

Site-Specific Nanobody Inhibitors of the Proteasomal Deubiquitinase UCH37.

Dysregulation of the ubiquitin (Ub) proteasome system (UPS) is linked to numerous human diseases, making its components, particularly deubiquitinases (DUBs), attractive therapeutic targets. UCH37 (also known as UCHL5), a proteasomal DUB, plays roles in protein degradation, DNA repair, and transcription and is overexpressed in several cancers. Despite its relevance, the precise functions of UCH37 within the proteasome and the INO80 chromatin remodeling complex remain unclear.

Medial-to-lateral vs. Lateral-to-medial: Determining the Optimal Approach in Laparoscopic Right Hemicolectomy-A Cohort Study.

Aim: To check the immediate and distant results of medial-to-lateral (ML) and lateral-to-medial (LM) methods to laparoscopic right hemicolectomy.

Methods: The present study is a retrospective cohort analysis of 453 laparoscopic right hemicolectomy procedures performed between 2018 and 2024 at a single Chinese tertiary care hospital, comparing LM and ML techniques. Rates of conversion, complications, lymph node yield, and survival were the main results.

Therapeutic effects of a pulsatile GnRH pump on adult male patients with congenital hypogonadotropic hypogonadism (CHH): a retrospective study.

Background: Traditional treatments for congenital hypogonadotropic hypogonadism (CHH) primarily focus on human chorionic gonadotropin (hCG) monotherapy, combined gonadotrophin treatment or Testosterone replacement therapy, which has certain limitations in effectiveness. In recent years, pulsatile gonadotropin-releasing hormone (GnRH) pump treatment, capable of mimicking physiological hormone release patterns, has become a more preferred treatment option. However, there is still a scarcity of medium- and long-term studies in this regard.

Engineered Extracellular Vesicles Enriched with miR-214 Enhance the Efficacy of Chemotherapy for Ovarian Cancer.

Recurrent ovarian cancer (OC) remains a major cause of mortality due to chemoresistance and metastasis. Epigenetic dysfunction, particularly involving microRNAs (miRNAs), contributes to disease progression. Extracellular vesicles (EVs) are key modulators of tumor epigenetic profiles by delivering bioactive cargo to recipient cells.

Prevention of donation-related infections: investigating the use of antibiotics in the decontamination of preservation fluid for organ transplantation.

Background: Donation-related infections (DRIs), particularly those caused by carbapenem-resistant gram-negative bacteria (CRGNB), can have disastrous consequences because of their extensive drug resistance. Contamination during graft acquisition and transport can lead to DRIs, and the use of antibiotics in preservation fluid (PF) before organ transplantation can reduce the incidence of DRIs. This study was to determine and compare the effectiveness of different PF decontamination regimens to prevent CRGNB related DRIs.

Zmiz1-Mediated SUMOylation of NLRP3 Inflammasome Regulates Satellite Glial Cell Activation and Neuronal Autophagy in Trigeminal Neuralgia.

Trigeminal neuralgia (TN) is characterized by neuroinflammation and satellite glial cell (SGC) activation, but the molecular mechanisms remain unclear. This study identifies zinc finger MIZ-type containing 1 (Zmiz1) as a key regulator that promotes SUMOylation of NLRP3 inflammasome, thereby influencing SGC activation and neuronal autophagy in TN. Using bioinformatics analysis, we identified Zmiz1 as a key SUMOylation-related gene involved in TN.

RNF213 regulates blood‒brain barrier integrity by targeting TRAF3 for type I interferon activation during A. baumannii infection.

RNF213 is the first identified susceptibility gene for moyamoya disease, and the encoded protein was recently recognized as a key antimicrobial protein. However, the function of RNF213 in host defense against brain infection remains unclear. Here, we show that increased expression of Rnf213 is significantly regulated by interferon alpha/beta receptor (IFNAR) signaling during bacterial infection and ligand stimulation.

Landscape analysis of matrix metalloproteinases reveals key prognostic markers for prostate cancer.

Background: Prostate cancer (PCa) is the most common male malignancy and significantly impairs patient's survival. Matrix metalloproteinases (MMPs) play a crucial role in tumor progression, yet the comprehensive role of MMPs in PCa remains unclear.

Method: Data from UCSC and GEO databases were firstly analyzed to evaluate expression characteristics, prognostic value, immune-cell infiltration, tumor-mutation burden (TMB), microsatellite-instability (MSI), immunotherapy sensitivity, and drug sensitivity in PCa.

Minimally invasive in situ bone extraction-implant implantation-bone augmentation by robot: a case report.

Objective: To present a technical process for robot-assisted, minimally invasive bone extraction-implant implantation-bone augmentation in patients with bone defects or bone atrophy.

Method: In this technical process, the surgical trajectory was preplanned within the robotic system. After patient registration and completion of robotic arm calibration, precise movement of the robotic arm enabled minimally invasive bone extraction using a trephine, followed by reaming with a single spiral drill.

The influence of exercise-induced fatigue on neuromuscular control strategies during lifting tasks: A muscle synergy study based on wavelet packet transform.

Repetitive lifting-related low back pain is a significant health issue among workers, in which fatigue is generally identified as a critical risk factor. However, there is currently a lack of research on the effects of exercised induced fatigue on neuromuscular control patterns, which can be identified through muscle synergy based on the signals of surface electromyographic (sEMG). This study employs a wavelet packet transform (WPT) combined with non-negative matrix factorization (NNMF) to investigate the lifting task from the perspective of muscle synergy and to explore the characteristics of muscle synergy patterns under exercise-induced fatigue.

Discovery of pentagalloylglucose in Moutan Cortex as a highly potent inhibitor of human peptidyl arginine deiminase 4 (PAD4) by UHPLC-MS/MS.

Peptidyl arginine deiminase 4 (PAD4) is an enzyme playing essential role in many biological processes especially immune responses, and is a highly attractive therapeutic target. However, the number of PAD4 inhibitors is limited, and there is no inhibitor in clinical trial. This study aims to identify natural compounds holding high inhibitory effect on PAD4 activity from traditional herbal medicine.

Boosting thermal conductivity of boron nitride incorporated polymer composites hydrogen bonding engineering.

Enhancing the thermal conductivity of polymer-based composites is critical for effective thermal management in power electronics. A common strategy involves incorporating high-thermal-conductivity fillers such as graphene and boron nitride nanosheets (BNNS). However, practical enhancements often fall short of theoretical predictions due to interfacial thermal resistance ().

Osteogenic and antibacterial enhancement by alloying design and microstructural modification of additively manufactured biodegradable metals.

Additively Manufactured metallic implants face a critical challenge in simultaneously promoting osteogenesis and preventing infection, two competing requirements in complex orthopedic applications such as implant-associated infections. This study presents a novel strategy combining Cu alloying and heat treatment for biodegradable zinc-based implants fabricated by laser powder bed fusion (L-PBF), in order to address infected bone repair. After alloying, the as-built Zn-2Cu implants showed limited enhancement compared to pure Zn due to the microstructure dominated by solid solution.

Inflammatory arthritis immune related adverse events represent a unique autoimmune disease entity primarily driven by T cells, but likely not autoantibodies.

Immune checkpoint inhibitor (ICI)-induced inflammatory arthritis (IA) is an increasingly recognized immune-related adverse event (irAE), yet its underlying immunopathogenesis remains poorly understood. Unlike rheumatoid arthritis (RA), where autoantibodies and B cell dysfunction are central features, the contribution of humoral immunity to IA irAE is unclear. Here, we performed in-depth immunophenotyping of peripheral blood from patients with IA irAE, and compared them with seronegative RA patients, ICI-treated patients without irAE, and healthy controls.

Identification of key genes and immune infiltration mechanisms in limb ischemia-reperfusion injury: a bioinformatics and experimental study.

Aim Of The Study: To establish a cross-tissue bioinformatics model for identifying conserved key genes and immune infiltration mechanisms in ischemia-reperfusion injury (IRI) with experimental validation in limb IRI, including pharmacological targeting of the WNT5A/PLC pathway.

Materials And Methods: Transcriptomic data from CTGF-stimulated cardiac myocytes (GSE36073) were analyzed as a surrogate for limb IRI due to shared pathological mechanisms. Random forest, LASSO regression, algorithms identified feature genes, validated in a rat limb IRI model using RT-qPCR, and histology.

Cytosolic Delivery of Functional Ubiquitin.

The proteostasis network involves complex protein signaling cascades. The tagging of proteins with ubiquitin is central to the degradation of cellular proteins, but understanding its exact role in processing proteins is complicated by the complexity and extent of its utilization within cells. Here, we describe the application of a traceless protein delivery strategy to effect the uptake of exogenous ubiquitin into the cytosol of human cells.

[Retracted] lncRNA‑MIAT regulates cell biological behaviors in gastric cancer through a mechanism involving the miR‑29a‑3p/HDAC4 axis.

Following the publication of the above article, a concerned reader drew to the Editor's attention that, regarding the cell invasion assay data shown in Fig. 2B on p. 3469, the 'si‑NC (SGC7901)' and 'si‑NC (MGC803)' data panels appeared to show the same data, albeit the panels were presented in a different orientation with respect to each other (rotated through 180°).

Enhancing the blast resistance of an elite thermo-sensitive genic male sterile line (TGMS) Longke638S and its derived hybrid varieties by incorporating gene.

Unlabelled: Rice blast, caused by the fungal pathogen , is one of the most destructive diseases of rice worldwide. The utilization of host resistance () genes in rice breeding program is considered as the most economical, effective, environment-friendly strategy for rice blast control. The gene , shows high, broad-spectrum and durable resistance to rice blast.

Effects of Reiki therapy on quality of life: a meta-analysis of randomized controlled trials.

Purpose: This review aimed to evaluate the therapeutic effects of Reiki therapy on quality of life.

Methods: The review followed standard scientific journal practices and a systematic search of PubMed, Web of Science, Embase, Scopus, and the Cochrane Library, with a literature cutoff of September 2024, was conducted to identify relevant studies. Inclusion criteria comprised articles published in English, randomized controlled trials (RCT), Reiki therapy as the independent variable, diverse patient populations, and outcome measures focusing on quality of life improvement.