triggers type I interferon signaling to promote host defense against infection via mTORC1 activation.

Multidrug-resistant , a prevalent opportunistic pathogen in hospitals, presents a substantial risk to immunocompromised patients, triggering life-threatening conditions including pneumonia, bacteremia, and sepsis, which can be fatal. Therefore, it is urgent to develop new therapeutic strategies to combat and eradicate multidrug-resistant infection. Our finding reveals that promotes the mice treated with 5-fluorouracil (5-FU) to defend against multidrug-resistant infection via the mTORC1-IFN-I signaling pathway.

Analysis of Non-Coding RNAs and N6-Methyladenosine-Modified Genes in Response to flg22 in Grape Immunity.

Grape (Vitis vinifera) is susceptible to fungal pathogens and, consequently, severe yield losses. Investigating the immune mechanisms of the disease-resistant Chinese wild grapes is crucial for developing sustainable disease resistance technologies. Here, we conducted whole-transcriptome and methylated-RNA-immunoprecipitation sequencing to elucidate the immune defence mechanisms underlying grapevine responses to bacterial flagellin 22 (flg22).

Blocking β-adrenergic receptor signaling enhances the sensitivity of AML to MCL-1 inhibitor S63845.

Aims: Myeloid cell leukemia-1 (MCL-1) critically drives acute myeloid leukemia (AML) pathogenesis and represents a promising therapeutic target. Despite potent preclinical activity, the clinical efficacy of S63845, a selective MCL-1 inhibitor, remains limited in AML trials. We therefore aimed to develop novel combination strategies enhancing S63845 sensitivity.

RNF213 regulates blood‒brain barrier integrity by targeting TRAF3 for type I interferon activation during A. baumannii infection.

RNF213 is the first identified susceptibility gene for moyamoya disease, and the encoded protein was recently recognized as a key antimicrobial protein. However, the function of RNF213 in host defense against brain infection remains unclear. Here, we show that increased expression of Rnf213 is significantly regulated by interferon alpha/beta receptor (IFNAR) signaling during bacterial infection and ligand stimulation.

How Health Self-Efficacy Affects Physical Activity Among Chinese Adults with Different COVID-19 Experiences: The Mediating Role of Health Information Technology Use.

The COVID-19 pandemic has profoundly impacted global health. Considering the benefits of physical activity (PA) for improving health, this study focuses on enhancing PA among groups with different COVID-19 experiences. Guided by self-efficacy and self-regulation theories, this study examines how health self-efficacy (HSE) increases PA, with using health information technology (IT) for self-regulation serving as a mediating factor across three groups: uninfected (Group 1), infected without symptoms (Group 2), and infected with symptoms (Group 3).

Risk of new-onset and recurrent uveitis with different biologics for ankylosing spondylitis: a network meta-analysis.

Background: Uveitis is a common extra-articular manifestation of ankylosing spondylitis (AS), and a systematic analysis of the effects of biologics on new-onset and recurrent uveitis is clinically important.

Methods: We conducted a network meta-analysis (NMA) to assess the impact of anti-TNF-α (adalimumab, etanercept, golimumab, and infliximab), IL-17 inhibitors (secukinumab, bimekizumab, and ixekizumab), and JAK inhibitors (tofacitinib and upadacitinib) on new-onset and recurrent uveitis. Phase II/III double-blind randomized controlled trials and cohort studies were included.

Spatial regulation effects of Taohong Siwu decoction on disordered lipids in a rat model of ischemic stroke assessed using mass spectrometry imaging.

Ischemic stroke serves as a disease closely related to cerebral blood vessels, with high mortality, disability, and recurrence rates. Growing studies have confirmed that lipid disturbances have a strong correlation with ischemic stroke damage. Taohong Siwu decoction (THSWD) as a classical remedy in Chinese pharmacopeia medicinal formula could promote blood circulation and eliminate stagnation of blood.

The influence of fitness technology use on cancer screening behaviors among American women: Mediation by social media communication and cancer worry (2017-2020).

Objective: This study investigates how fitness technology (FT) influences cancer screening behaviors among American women, focusing on the role of social media communication and cancer worry as mediators.

Methods: We used data from 9245 women across four cycles (2017-2020) of the Health Information National Trends Survey. Descriptive analyses, one-way ANOVA, and sequential mediation analysis were conducted to assess the relationship between FT use and cancer screening behaviors.

The transcription factor bHLH77 promotes the expression of stilbene synthase gene family member STS48 and stilbene biosynthesis by stabilizing MYB15 in Vitis quinquangularis.

Stilbenes are crucial phytoalexins in plants that can protect against pathogenic invasions. Grapevines, in particular, contain significant amounts of stilbenes. The stilbene synthase (STS) genes have been shown to facilitate the accumulation of these stilbenes.

miR3398-VqMYB15 Regulates the Synthesis of Stilbene in Vitis quinquangularis.

Grapevine (Vitis vinifera L.) is an economically important fruit crop grown worldwide. Grapevine is cultivated extensively in China, and certain wild grapes exhibit excellent resistance to pathogens and stress.

Semi-rational evolution of a recombinant DNA polymerase for modified nucleotide incorporation efficiency.

Engineering improved B-family DNA polymerases to catalyze 3'-O-modified nucleotide reversible terminators is limited by an insufficient understanding of the structural determinants that define polymerization efficiency. To explore the key mechanism for unnatural nucleotide incorporation, we engineered a B-family DNA polymerase from Thermococcus Kodakaraenis (KOD pol) by using semi-rational design strategies. We first scanned the active pocket of KOD pol through site-directed saturation mutagenesis and combinatorial mutations and identified a variant Mut_C2 containing five mutation sites (D141A, E143A, L408I, Y409A, A485E) using a high-throughput microwell-based screening method.

Recent advancements in antibiotics removal by bio-electrochemical systems (BESs): From mechanisms to application of emerging combined systems.

Recent advancements in bio-electrochemical systems (BESs) for antibiotic removal are receiving great attentions due to the electro-active bacteria on the electrode that could elevate the removal efficiency. Enhanced detoxification performance of BESs compared to the traditional biological processes indicates the great potential serving as a sustainable alternative or a pre-/post-processing unit to improve the performance of biological processes. However, the successfully application of BESs to antibiotic-polluted water remediation requires a deeper discussion on their operational performance and emerging coupled systems.

Artemisia argyi polysaccharide alleviates osmotic diarrhea by enhancing intestinal barrier protection and anti-inflammation.

Artemisia argyi polysaccharide (AAP) is a homogeneous polysaccharide with a molecular weight of 16 kDa, displaying anti-inflammatory, antioxidant, and anti-tumorigenic properties, and potential protective effects on intestinal barrier function. It is anticipated to serve as an efficient component in diarrhea treatment. This study aims to examine the impact of AAP on diarrhea severity, intestinal barrier function, and inflammation in diarrhea-induced rats.

Insights into ubiquitinome dynamics in the host‒pathogen interplay during Francisella novicida infection.

Ubiquitination functions as an important posttranslational modification for orchestrating inflammatory immune responses and cell death during pathogenic infection. The ubiquitination machinery is a major target hijacked by pathogenic bacteria to promote their survival and proliferation. Type I interferon (IFN-I) plays detrimental roles in host defense against Francisella novicida (F.

Rapid detection of murine-derived ingredients in meat products using real-time enzymatic recombinase amplification.

Background: Accurate identification of meat species is critical to prevent economic fraud and safeguard public health. The use of inappropriate meat sources, such as murine, poses significant health risks because of potential contamination with pathogens and allergens, leading to foodborne illnesses. The present study aimed to develop a novel real-time enzymatic recombinase amplification (ERA) method for the rapid and specific detection of murine DNA in meat products.

Synthesis of histidine, serine and folic acid-functionalized and boron and iron-doped graphene quantum dot with excellent optical behavior and peroxidase-like activity for colorimetric and fluorescence detection of HO in food.

Low fluorescence under visible light excitation and catalytic activity limit many applications of graphene quantum dots in optical detection, biosensing, catalysis and biomedical. The paper reports design and synthesis of histidine, serine and folic acid-functionalized and boron and iron-doped graphene quantum dot (Fe/B-GQD-HSF). The Fe/B-GQD-HSF shows excellent fluorescence behavior and peroxidase-like activity.

Association of Vegetables-Fruits Dietary Patterns with Gestational Diabetes Mellitus: Mediating Effects of Gut Microbiota.

(1) Introduction: Previous studies have found that diet can change gut microbiota, thereby affecting metabolic health. However, research on gestational diabetes mellitus (GDM) is still limited. Our study aimed to explore the mediating role of gut microbiota in the relationship between dietary patterns and GDM.

NCF4 attenuates colorectal cancer progression by modulating inflammasome activation and immune surveillance.

The spatiotemporal regulation of inflammasome activation remains unclear. To examine the mechanism underlying the assembly and regulation of the inflammasome response, here we perform an immunoprecipitation-mass spectrometry analysis of apoptosis-associated speck-like protein containing a CARD (ASC) and identify NCF4/1/2 as ASC-binding proteins. Reduced NCF4 expression is associated with colorectal cancer development and decreased five-year survival rate in patients with colorectal cancer.

Sirt1 inhibits macrophage polarization and inflammation in gouty arthritis by inhibiting the MAPK/NF-κB/AP-1 pathway and activating the Nrf2/HO-1 pathway.

Objective And Design: To elucidate Sirt1's role in gouty arthritis inflammation and its potential mechanisms.

Material: Constructed murine models of gouty arthritis and conducted THP-1 cell experiments.

Treatment: 1 mg of MSU crystals injected into mice ankle joints for a 72-h intervention.

Comprehensive assessment of the microbial community structure in a typical lead-zinc mine soil.

Understanding the microbial community structure in soil contaminated with heavy metals (HMs) is a precondition to conduct bioremediation in mine soil. Samples were collected from a typical lead-zinc (Pb-Zn) mine to assess the microbial community structure of the HMs concentrated in the soil. The goal was to analyze the bacterial and fungal community structures and their interactions using the 16S rRNA genes and internal transcribed spacer high-throughput sequencing.

Ultra-performance liquid chromatography-quadrupole time-of-flight mass combined with UNIFI to study the mechanism of Tao Hong Si Wu Decoction in the treatment of postpartum blood stasis.

Postpartum hemorrhage can lead to a variety of maternal complications. Tao Hong Si Wu Decoction (THSWD) is a traditional Chinese medicine used for treating gynecological diseases. However, the active ingredients of THSWD and its pharmacological mechanism of treatment for postpartum blood stasis still remained unclear.

Designing of multifunctional graphene quantum dot-polyvinyl alcohol-polyglycerol luminescent film for fluorescence detection of pH in sweat.

Background: Wound infection, skin disease, renal failure, cancer, cystic fibrosis, and other pathologies may induce obvious pH changes in sweat. Thus, tracking skin pH changes can help monitor human health in a convenient manner. Owing to their biocompatibility, easy preparation, and sensitive response to pH changes, graphene quantum dots (GQDs) have received increased attention in the optical detection of pH changes.

PHB2 Alleviates Neurotoxicity of Prion Peptide PrP via PINK1/Parkin-Dependent Mitophagy.

Prion diseases are a group of neurodegenerative diseases characterized by mitochondrial dysfunction and neuronal death. Mitophagy is a selective form of macroautophagy that clears injured mitochondria. Prohibitin 2 (PHB2) has been identified as a novel inner membrane mitophagy receptor that mediates mitophagy.