DDX55 safeguards naïve T cell homeostasis by suppressing activation-promoting transposable elements.

Naïve T cells are maintained in a homeostatic state to preserve a stable T cell pool with diverse T cell receptor (TCR) repertoires, ensuring preparedness for priming. However, the underlying mechanisms controlling naïve T cell homeostasis and priming remain unclear. Leveraging a machine learning-based functional genetic screen, we identified () as the top factor responsible for naïve T cell homeostasis.

[Rapid determination of five antipyretic analgesics in surface water by online solid phase extraction and ultra-high performance liquid chromatography-tandem mass spectrometry].

Antipyretic analgesics are typical pharmaceutical and personal care products （PPCPs） that are widely used in our daily life because they relieve fever and pain， and have anti-inflammatory and anti-rheumatic properties. These drugs inhibit the synthesis and release of prostaglandins （PGs） in the neurons of the anterior hypothalamus and exert therapeutic effects as a consequence. However， these drugs are relatively commonly misused and abused， often owing to a lack of proper medication guidance.

Clinical efficacy of simethicone combined with bifidobacterium in the treatment of pediatric aerophagia.

Background: The causes of children aerophagia are complex, and the treatment methods are diverse. Moreover, it is prone to recurrent attacks. Simethicone oil, as a safe and new type of drug, can be used for treatment.

Temporal and spatial pattern, sources, and main controlling factors of odor compounds in Qiandaohu Reservoir.

The ubiquitous presence of algal-derived odors in large lakes and reservoirs constitutes a critical environmental concern. Particularly during the high-temperature summer months, localized regions of Qiandaohu Reservoir have exhibited markedly elevated concentrations of odor compounds. To investigate the intricate relationships among odor compounds, algae communities, and environmental factors in Qiandaohu Reservoir, and to forestall the impact of odor compounds on the quality of drinking water, this research implemented a systematic monthly survey to monitor several types of odor compounds, algal communities, physicochemical factors, and associated synthetases.

Visceral Adiposity Thresholds for Cardiovascular Risk Stratification: A Simplified Biomarker-Driven Model.

Objective: VAT volume is a critical determinant of cardiometabolic risk, yet population-specific thresholds and accessible predictive tools remain undefined.

Methods: Using US National Health and Nutrition Examination Survey (NHANES) data, we analyzed dual-energy x-ray absorptiometry (DXA)-derived VAT volume. Threshold effects and sex/ethnicity interactions were evaluated via multivariable regression, while LASSO regularization and ROC analyses identified a simplified predictive model.

Multi-omics approach reveals CCND1, GABPA, HIF1A, and SOX6 as key regulators and prognostic markers in heart failure.

Introduction: Heart failure (HF) is a progressive condition with complex molecular mechanisms. This study aims to identify potential biomarkers and therapeutic targets by analyzing differentially expressed genes (DEGs) in HF patients, exploring the roles of hub genes, and developing a risk model for predicting disease progression.

Methodology: We cultured five human HF cell lines and five normal coronary cardiomyocyte cell lines.

Sleep Deprivation Aggravates Periodontitis Through Trigeminal-Periodontal Neuroimmune Pathway Mediated by the AChE-ACh-α7nAChR Axis.

Continuous sleep deprivation (SD) triggers systemic inflammatory storm and immune dysregulation, yet its specific impact on periodontitis and the corresponding therapeutic interventions remains unclear. Consequently, this study elucidates the neuroimmune mechanisms linking SD to ligature-induced periodontitis (LIP) in mice and evaluates electroacupuncture (EA) as a novel adjunctive therapy. Screening analyses (ELISA, public databases, flow cytometry, immunofluorescence, etc.

Optimizing core needle biopsy specimen collection techniques to enhance molecular testing adequacy in non-small cell lung cancer.

Background: Core needle biopsy (CNB) is commonly used for histological diagnosis and molecular testing in patients with non-small cell lung cancer (NSCLC). However, the adequacy of CNB specimens is often compromised by low tumor cell content or insufficient nucleic acid quantity, which can negatively impact the success of downstream analyses. Moreover, variations in specimen collection techniques may further contribute to inconsistency in sample quality.

A Spatial and Temporal Transcriptomic Atlas of Mouse Intestinal Regeneration.

Background & Aims: The intestinal epithelium exhibits a remarkable capacity for regeneration following injury. However, the spatial and temporal dynamics of the injury-repair cycle remain incompletely understood.

Methods: We employ spatial transcriptomics to create an atlas of the damage and repair response to ionizing radiation in the mouse intestine.

The impacts of removing pharmaceutical co-payments for chronic conditions at primary care level: a pilot study in rural China.

The underutilization of primary care (PC) presents a substantial challenge in enhancing the people-centeredness, quality, and efficiency of health services for patients with chronic diseases. Pharmaceutical copayments have been considered a key barrier to patient access in low- and middle-income countries. It is unclear whether the removal of pharmaceutical copayment can lead to better care and management of chronic diseases.

Effect of preheating conditions on UV laser direct coloring of stainless steel surfaces.

Metal surface laser coloring technology has attracted much attention because of its wide range of applications and significant advantages. The principle is based on the interaction between the laser and the metal surface, through the modulation of laser parameters (such as wavelength, power, and pulse width) to induce oxidation, microstructural reconstruction, or the equipartitional excitation resonance effect of the metal surface layer, so as to achieve accurate and controllable color generation. Current research focuses on multi-material compatibility, color stability, and dynamic regulation mechanisms, with the combination of nanostructured design and ultrafast laser technology further expanding the color gamut and resolution.

Cognitive Frailty and Health Care Utilization and Costs Among Middle-Aged and Older Adults: Findings from Four Longitudinal Cohort Studies.

Health care systems need better strategies to identify older adults at risk for costly care to select target populations for interventions to reduce health care burden. Cognitive impairment and frailty are among the two most common geriatric syndromes. We examined the association between cognitive frailty and health care utilization and costs.

Conformation-Induced Ion Transport "Channels" in Artificial Polymer Solid Electrolyte Interphase for Aqueous Zn-Metal Batteries.

Artificial polymer solid electrolyte interphases (SEIs) are crucial for inhibiting side reactions and regulating Zn flux in aqueous Zn-metal batteries, where tuning the affinities and bond types between Zn and functional groups is considered the most effective strategy. However, the underlying determinant-polymer conformation control-is often overlooked. In this work, it is discovered that a high exposure degree of zincophilic sites is the prerequisite for establishing interaction.

Ginkgolic acid as a fusion inhibitor: targeting RBD-ACE2 interaction with in silico, in vitro evidence of allosteric modulation and potent virucidal effect.

Background: This study investigates Ginkgolic acid, a naturally occurring phenolic compound from Ginkgo biloba, for its potential to inhibit SARS-CoV-2 by targeting the RBD-ACE2 interface through a combination of computational and methodologies.

Methods: In silico molecular docking and 500 ns molecular dynamics (MD) simulations were employed to examine the binding conformation and stability of Ginkgolic acid at the RBD-ACE2 interface. studies included cytotoxicity profiling, plaque reduction assays, qRT-PCR, luciferase-based viral entry quantification, and membrane fusion inhibition in Vero E6 and ACE2-expressing HEK293T cells.

Streptococcus pneumoniae synchronizes the states of cell wall peptidoglycan acetylation and genome methylation by programmed DNA inversions.

Bacterial cell wall peptidoglycan (PG) consists of alternating β-(1,4) linked N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG). The C-6 hydroxyl group of NAM is acetylated by transmembrane O-acetyltransferases post PG biosynthesis in many pathogenic bacteria. This modification is important for bacterial resistance to lysozyme.

Epidemiology of herpes zoster and post-herpetic neuralgia in China: A nationwide population-based survey.

Objectives: Herpes zoster (HZ) and post-herpetic neuralgia (PHN) cause substantial burden worldwide, whereas China lacks nationwide statistics of HZ incidence. This study aims to provide national epidemiology profile for HZ and PHN in China.

Methods: A nationwide population-based survey was conducted in China in 2024.

Glucokinase activator, circulating metabolites, and cardio-cerebrovascular diseases: a Mendelian randomization study.

Background: Glucokinase activator (GKA) has shown potential in influencing cardio-cerebrovascular diseases (CVDs), yet its metabolic mechanism remains unclear. Circulating metabolites play a significant role in the etiology of CVDs. We hypothesized that the effects of GKA on cardio-cerebrovascular outcomes are mediated through circulating metabolites.

The Effects of Soil Cover Thickness on Leaf Functional Traits of Vine Plants in Mining Areas Depend on Soil Enzyme Activities and Nutrient Cycling.

Understanding the interplay between plant leaf functional traits and plant and soil factors under different soil thicknesses is significant for quantifying the interaction between plant growth and the environment. However, in the context of ecological restoration of vegetation in mining areas, there has been a lot of research on trees, shrubs, and grasses, but the characteristics and correlations of leaf functional traits of vines have not been fully studied to a large extent. Here, we report the differences in leaf functional traits of six vine plants (, , , , , and ) with distinct growth forms in different soil cover thicknesses (20 cm, 40 cm, and 60 cm).

Efficacy and safety of 35 mg of recombinant human prourokinase for thrombolysis in acute ischemic stroke: a meta-analysis of randomized controlled trials.

Background: Compared with alteplase, recombinant human prourokinase (rhPro-UK)-a next-generation specific plasminogen activator-offers advantages such as weight-independent dosing and cost effectiveness. While a 35-mg dose of rhPro-UK has been recommended in previous randomized controlled trials (RCTs), its efficacy and safety profile have yet to be fully elucidated, as no relevant systematic reviews or meta-analyses have been conducted to date.

Aim: This meta-analysis aimed to evaluate the safety and efficacy of 35 mg of rhPro-UK compared with those of control treatments, including 50 mg of rhPro-UK and alteplase monotherapy, in patients with acute ischemic stroke (AIS).

The morphology, genetic structure, and haplotype distribution of the invasive freshwater snails Biomphalaria straminea and Physa acuta in Guangdong, China.

Background: Biomphalaria and Physa (order Gastropoda) serve as vectors and reservoirs for infectious agents that affect both humans and animals. This study provides updated insights into the epidemiology, morphology, phylogeny, and haplotype diversity of Biomphalaria and Physa snails in Guangdong Province, southern China.

Methods: Field surveys were conducted across Guangdong Province from 2016 to 2023.

Exploratory phase II trial of an anti-PD-1 antibody camrelizumab combined with a VEGFR-2 inhibitor apatinib and chemotherapy as a neoadjuvant therapy for triple-negative breast cancer (NeoPanDa03): efficacy, safety and biomarker analysis.

Chemotherapy serves as the primary therapeutic approach for triple-negative breast cancer (TNBC), yet its efficacy remains unsatisfactory. This study was a single-arm, open-label, single-center clinical trial (NCT05447702) involving patients with newly diagnosed stage II-III TNBC at West China Hospital. The treatment regimen consisted of camrelizumab (200 mg intravenously every 2 weeks, 12 cycles), apatinib (250 mg orally daily), and alternating chemotherapy [nab-paclitaxel (d1, 8, 15 every 4 weeks) for 4 cycles and epirubicin plus cyclophosphamide (every 2 weeks) for 4 cycles].

Factors influencing the difficulty of video-assisted thoracoscopic anterior mediastinal tumor resection via the subxiphoid approach.

Background: Video-assisted thoracoscopic mediastinal tumor resection via the subxiphoid approach (SVATS) is the mainstream surgical method for treating anterior mediastinal tumors. The impact of thoracic and mediastinal size on surgery has long been described only as a subjective feeling, and no related research has been reported. In this study, we retrospectively investigated the preoperative predictive factors affecting SVATS, focusing on the depth of the anterior mediastinum and thoracic morphology (width and depth).

The role of Quercetin in the treatment of kidney diseases: A comprehensive review.

Kidney diseases resulting from genetic predisposition, immune dysfunction, hypertension, diabetes, malignancies, hyperuricemia, and unhealthy lifestyle factors are garnering increasing attention due to the essential role of the kidney in maintaining systemic homeostasis. Quercetin (QCT), a polyhydroxy flavonoid abundantly present in the flowers, leaves, and fruits of numerous plants, including over 100 species of Chinese herbs such as Sophora japonica and Tansy, exhibits diverse biological activities and significant medicinal properties. These properties include antioxidant, anticancer, anti-inflammatory, antibacterial, antiviral, hypoglycemic, antihypertensive, immunomodulatory, and cardiovascular protective effects.