Identifying rosmarinic acid as a color stabilizer for tuna meat based on virtual screening and in vitro evaluation.

This paper aims to identify the potential of natural polyphenolic compounds as color stabilizers for tuna meat through virtual screening and in vitro study. A virtual screening database was constructed to identify polyphenols with high binding affinity to myoglobin (Mb), and the interaction mechanism between polyphenols and Mb was also studied. The results demonstrated that tuna meat treated with rosmarinic acid (RA), which exhibits strong binding force to Mb, showed a significantly higher a*/b* value of 0.

TPOT: TOPOLOGY PRESERVING OPTIMAL TRANSPORT IN RETINAL FUNDUS IMAGE ENHANCEMENT.

Retinal fundus photography enhancement is important for diagnosing and monitoring retinal diseases. However, early approaches to retinal image enhancement, such as those based on Generative Adversarial Networks (GANs), often struggle to preserve the complex topological information of blood vessels, resulting in spurious or missing vessel structures. The persistence diagram, which captures topological features based on the persistence of topological structures under different filtrations, provides a promising way to represent the structure information.

Evaluating the effects of liraglutide, empagliflozin and linagliptin on mild cognitive impairment remission in patients with type 2 diabetes (LIGHT-MCI): study protocol for a multicentre, randomised controlled trial with an extension phase.

Introduction: Diabetes is a significant modifiable risk factor for cognitive dysfunction. There are currently no effective treatments that delay or reverse the progression of cognitive dysfunction. Accumulating evidence demonstrates that specific antidiabetes medications hold promise in improving cognitive function.

Effect of plasma-activated water combined with low-voltage electric field-assisted thawing on the quality and protein structure of spotted sea bass.

The non-thermal processing technology of low-temperature plasma-activated water combined with a low-voltage electric field (PAW-LVEF) was employed to study the thawing characteristics and physicochemical properties of spotted sea bass. The results showed that PAW-LVEF assisted-thawing (PAW-LVEFT) improved the thawing rate compared to the other three thawing methods. The muscle tissue structure was better maintained and thus the water-holding capacity was enhanced.

Aloe-emodin ameliorates chronic kidney disease fibrosis by inhibiting PI3K-mediated signaling pathway.

Chronic kidney disease (CKD) impacts a vast number of individuals worldwide, culminating in renal fibrosis. Renal fibrosis serves as the main reason for end-stage renal failure. However, the current targeted treatment methods for renal fibrosis remain scarce.

Multimodal Variational Autoencoder: A Barycentric View.

Multiple signal modalities, such as vision and sounds, are naturally present in real-world phenomena. Recently, there has been growing interest in learning generative models, in particular variational autoencoder (VAE), for multimodal representation learning especially in the case of missing modalities. The primary goal of these models is to learn a modality-invariant and modality-specific representation that characterizes information across multiple modalities.

ERK-METTL3 axis acts as a novel regulator of antiviral innate immunity combating pseudorabies virus infection.

The ERK-mediated phosphorylation of the core m6A methyltransferase METTL3 has been linked to the regulation of embryonic stem cell differentiation and tumorigenesis. However, its role in the antiviral innate immune response remains unclear. In this study, we found that during infection with the prototypical alpha-herpesvirus Pseudorabies virus (PRV), ERK2 protein expression increased significantly, while METTL3 expression decreased both in vitro and in vivo.

EVIT-UNET: U-NET LIKE EFFICIENT VISION TRANSFORMER FOR MEDICAL IMAGE SEGMENTATION ON MOBILE AND EDGE DEVICES.

With the rapid development of deep learning, CNN-based U-shaped networks have succeeded in medical image segmentation and are widely applied for various tasks. However, their limitations in capturing global features hinder their performance in complex segmentation tasks. The rise of Vision Transformer (ViT) has effectively compensated for this deficiency of CNNs and promoted the application of ViT-based U-networks in medical image segmentation.

Context-Aware Optimal Transport Learning for Retinal Fundus Image Enhancement.

Retinal fundus photography offers a non-invasive way to diagnose and monitor a variety of retinal diseases, but is prone to inherent quality glitches arising from systemic imperfections or operator/patient-related factors. However, high-quality retinal images are crucial for carrying out accurate diagnoses and automated analyses. The fundus image enhancement is typically formulated as a distribution alignment problem, by finding a one-to-one mapping between a low-quality image and its high-quality counterpart.

Chromosome-level genome assembly of Marco Polo Blister Beetle (Hycleus marcipoli).

Hycleus marcipoli is an agricultural pest that feeds on the flowers and leaves of leguminous plants, including Desmodium spp., as well as sweet potatoes. It exhibits hypermetamorphic development-an exceptionally complex life cycle shared by the genus and subfamily.

A novel thermo-activated one-pot RPA-CRISPR-Cas12b assay for Mycoplasma pneumoniae POCT.

Mycoplasma pneumoniae (M. pneumoniae), a major human respiratory pathogen, necessitates the development of rapid point-of-care testing (POCT) platforms for clinical management. However, current two-step workflows suffer from operational complexity and aerosol contamination risks.

Mitogenomics provide insights into the tribe-level systematics and historical phylogeography of band-winged grasshoppers (Orthoptera: Acrididae: Oedipodinae).

Oedipodinae (Acrididae) is a species-rich and globally distributed subfamily of grasshoppers, currently comprising 807 valid species assigned to 138 genera in 16 tribes. Resolving the phylogeny of Oedipodinae has proven difficult, owing to their extensive species diversity, disjunct geographic distribution and the scarcity of informative molecular markers. To establish a more robust phylogenetic framework, we conducted a mitochondrial phylogenomic analysis of 143 mitogenomes.

Piezo1 induces mitochondrial autophagy dysfunction leading to cartilage injury in knee osteoarthritis.

Background: External mechanical stress plays a pivotal role in the pathogenesis of knee osteoarthritis. Piezo1 can sense mechanical stress changes on the surface of various cell types and convert them into bioelectrical signals to regulate cellular functions. Therefore, our study aimed to investigate the role of Piezo1 in mechanically induced KOA and elucidate its underlying mechanisms.

Epidemiology of herpes zoster and post-herpetic neuralgia in China: A nationwide population-based survey.

Objectives: Herpes zoster (HZ) and post-herpetic neuralgia (PHN) cause substantial burden worldwide, whereas China lacks nationwide statistics of HZ incidence. This study aims to provide national epidemiology profile for HZ and PHN in China.

Methods: A nationwide population-based survey was conducted in China in 2024.

Comparative Analysis of Codon Usage Bias in Transcriptomes of Eight Species of Formicidae.

Ants are among the most widely distributed eusocial insects, and desert ants, in particular, serve as important model organisms for studying animal navigation. : In this study, we provide high-quality de novo transcriptomes for eight ant species: (Nylander, 1849), Wheeler, 1933, Santschi, 1937, (Emery, 1901), Zhu, Wu, Duan & Xu, 2022, Wheeler, 1927, Wheeler, 1927, and Emery, 1925. : The GC content of coding sequences (CDSs) ranged from 43.

Current Status of Grain Drying Technology and Equipment Development: A Review.

Grain drying technology is a core process for ensuring food quality, extending storage life, and improving processing adaptability. With the continuous growth of global food demand and the increasing requirements for food quality and energy efficiency, traditional drying technologies face multiple challenges. This paper reviews six major grain drying technologies, comprising hot air drying, microwave drying, infrared drying, freeze drying, vacuum drying, and solar drying.

Deciphering the genomic characters of Ptygonotus chinghaiensis, a high-altitude grasshopper endemic to the Qinghai-Tibet Plateau, using low-coverage short-read sequencing.

Background: The Qinghai-Tibet Plateau, the world's youngest plateau, harbors a unique biodiversity shaped by its extreme environmental conditions. Genomic adaptations in its endemic insects, particularly those with large genomes such as grasshoppers (5.6-20 Gb), remain underexplored.

Characterization of aroma profiles and microbial communities of cigar tobacco leaves from different varieties and origins and their correlations analysis.

Microorganisms play an important role in cigar tobacco leaves (CTLs) production, while the impact of varieties and origins on microorganisms and thereby on aroma profiles were rarely reported. In this study, the aroma profiles of wrapper and filler CTLs from three varieties, respectively, and filler CTLs from four origins were analyzed, and the bacterial and fungal communities of these CTLs and their correlation with aroma constituents were explored. The results showed that ketones and nitrogen heterocycles were dominant in wrapper and filler CTLs of different varieties and filler CTLs from different origins, their proportions in different CTLs were 22.

Astrocytic-HSP60 Depletion Contributed to Autophagy Defects of Astrocytes and Depressive-Like Behaviors in Male Mice.

Depression, a prevalent mental health disorder, is multifaceted in its etiology. Growing evidence suggests that dysregulation of heat shock protein 60 (HSP60) contributes to neurological dysfunction, but its role in astrocyte-mediated depressive-like behaviors and neuroinflammation remains poorly understood. Here, we sought to investigate whether astrocyte-specific HSP60 depletion disrupts cellular homeostasis and is associated with astrocyte dysfunction that contributes to depressive-like behaviors and related inflammatory signaling, with a particular emphasis on the role of autophagy.

In-Situ Atomic-Scale Revelation of Amorphous Metallic Iron Formation during Hydrogen-Driven Reduction of Iron Oxides.

The transition to hydrogen as a green reductant in metal production is critical for decarbonizing the metallurgical industry, yet atomic-scale mechanisms governing reduction pathways and phase evolution remain unresolved. Using in situ environmental transmission electron microscopy, we identify a hidden pathway that reveals dynamic formation of amorphous metallic iron (Fe) during the hydrogen-driven reduction of ferrous oxides of FeO and FeO. Real-time imaging uncovers three coexisting transformation routes: (i) FeO → FeO, (ii) FeO → amorphous Fe, and (iii) FeO → amorphous Fe.

Effectiveness of symptom monitoring on electronic patient-reported outcomes (ePROs) among patients with lung cancer: a systematic review and meta-analysis.

Symptom monitoring using electronic patient-reported outcomes (ePROs) has demonstrated benefits for patients with cancer, yet the systematic effects for lung cancer remains unknown. This study performed a literature search in Medline, Embase, Cochrane library, CINAHL and APA PsyInfo before April 23rd, 2025, and identified 5755 papers. 18 (0.

Amino Acid Residue 131 Plays a Key Role in the Catalytic Performance of Nattokinase.

Molecular engineering of nattokinase has led to variants with improved catalytic efficiency, shedding light on its structure-function relationship. In this study, the web-based tool PoPMuSiC 2.1, which predicts protein stability changes upon mutation, was utilized to assess the catalytic characteristics of nattokinase.

A dual-species fermentation strategy enhances the flavor profile and hepatoprotective effects of blueberry puree against alcohol induced liver injury by modulating bioactive compound composition in mice.

Blueberries are widely recognized as both nutritious and delicious fruits full of various metabolites. However, due to their short shelf life, the taste and functional components of blueberries may undergo significant changes after storage. Therefore, a new fermentation method was explored to produce high-quality blueberries in this study.

METTL3 regulates PRRSV replication by suppressing interferon beta through autophagy-mediated IKKε degradation.

Methyltransferase-like-3 (METTL3)-mediated N6-methyladenosine (mA) modification of messenger RNAs plays a pivotal role in regulating innate immune responses, either promoting or combating virus replication. However, the biological function of METTL3 during porcine reproductive and respiratory syndrome virus (PRRSV) infection remains unclear. In this study, we found that PRRSV infection reprograms mA modifications in cellular transcripts, enhances METTL3 expression, and alters its subcellular distribution.