Efficacy and toxicity of PD-1 inhibitor combined with induction chemotherapy for locally advanced laryngeal and hypopharyngeal cancers.

Objective: To evaluate the efficacy, toxicity, and voice rehabilitation outcomes of PD-1 inhibitors combined with induction chemotherapy (PCIC) compared to induction chemotherapy (IC) alone.

Methods: A retrospective analysis was conducted on 250 patients with stage III/IVA squamous cell carcinoma of the larynx/hypopharynx treated between June 2021 and December 2023. After 1:1 propensity score matching, 216 patients (108 per group) were analyzed.

Polymorphonuclear myeloid-derived suppressor cells protect against hyperoxia-induced bronchopulmonary dysplasia in neonatal mice through suppression of excessive inflammatory response.

Background: Bronchopulmonary dysplasia (BPD), which primarily affects premature infants, is characterized by impaired lung development, reduced alveolarization, and chronic inflammation, leading to long-term respiratory complications. However, clinical prevention treatment of BPD remains challenging. Because immune cells may have a role in BPD pathogenesis and prevention, we investigated whether polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) protect against hyperoxia-induced BPD in a neonatal mouse model.

Diagnostic efficacy of fecal-based miR-92a for advanced colorectal neoplasia: a prospective multicenter screening trial.

Background: More efficacious, noninvasive screening methods are needed for advanced colorectal neoplasia. miR-92a is a reliable and reproducible biomarker for early colorectal cancer detection in stool samples. We compared the diagnostic efficacies of miR-92a, immunochemical fecal occult blood testing (FIT), and their combination (FIT + miR-92a) in a prospective multicenter screening trial.

Machine learning-based fusion model for predicting HER2 expression in breast cancer by Sonazoid-enhanced ultrasound: a multicenter study.

Purpose: To predict human epidermal growth factor receptor 2 (HER2) expression in breast cancer (BC) using Sonazoid-enhanced ultrasound in a machine learning-based model.

Materials And Methods: Between August 2020 and February 2021, patients with breast cancer who underwent surgical treatment without neoadjuvant chemotherapy were prospectively enrolled from 17 hospitals in China. HER2 expression status was assessed by immunohistochemistry or fluorescence hybridization (FISH).

Coordination of SLC39A1 and DRP1 facilitates HCC recurrence by impairing mitochondrial quality control.

Background: Despite rapid advances in HCC therapy, surgical resection is still the most effective treatment. However, postoperative relapse develops in a large population and the mechanism remains to be explored.

Methods: HCC resection samples were retrospectively collected from 12 nonrelapsed and 15 relapsed HCC patients for RNA sequencing.

Weight-adjusted waist index and deficit accumulation frailty trajectories in middle-aged and older adults: a longitudinal study.

Introduction: Previous studies indicated that obesity defined by BMI accelerate frailty, but this effect weakens when stratified by metabolic status. The Weight-adjusted Waist Index (WWI), a better indicator of central obesity, may provide a more accurate measurement. The primary aim of this study was to estimate the impact of WWI on frailty progression and compare its effects across different metabolic statuses.

Inhibition Peroxiredoxin-2 by Capsaicin Ameliorates Rheumatoid Arthritis via ROS-Mediated Apoptosis in Fibroblast-Like Synoviocytes.

Rheumatoid arthritis (RA), a prevalent and incurable autoimmune disease globally, is characterized by the immune system attacking the body's own tissues, leading to joint inflammation and damage. Capsaicin (CAP), from ., is known for its burning sensation-inducing property and has shown various pharmacological effects, yet its specific mechanisms and targets in RA treatment remain largely unclear.

Proteomic and metabolomic dissection of the V600E mutation-induced cellular state transition in lung epithelial cells.

Non-small cell lung cancer (NSCLC) is the most common subtype of lung cancer, and 4% of NSCLC patients are reported to harbor mutations in , which encodes a serine/threonine kinase capable of activating the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway. However, the role and effects of mutation in NSCLC or lung lineage cells are not fully understood. To mimic the naturally occurring V600E mutation in lung cancer, a V600E knock-in BEAS-2B cell model was established using CRISPR/Cas9.

Inhibition of TPI1 Sensitizes Cisplatin-Resistant Oral Cancer to Ferroptosis.

Iron metabolism has emerged as a critical factor in cancer biology, with elevated intracellular iron levels contributing to increased oxidative stress and tumorigenesis. However, the molecular determinants governing ferroptosis sensitivity remain incompletely understood. Triosephosphate isomerase 1 (TPI1), a key glycolytic enzyme, has been implicated in cancer progression, but its role in ferroptosis regulation, particularly in the context of chemoresistance, is largely unexplored.

Establishment and application of a new method for the detection of drug antibodies by flow cytometry.

Background: The drug antibodies can seriously affect the efficacy of the drug and/or destroy red blood cells (RBCs). In severe cases, the drug antibodies may lead to hemolytic anemia, ineffective transfusion, and even life-threatening conditions. However, current methods for detecting drug-induced antibodies present several limitations: cumbersome operational workflows, time-consuming processes, suboptimal sensitivity for low-titer antibodies, and ease of disturbance by autoantibodies and erythrocyte alloantibodies.

Increasing S1P promotes M1 macrophage in chronic obstructive pulmonary disease and chronic obstructive pulmonary disease-obstructive sleep apnea overlap syndrome via S1PR1/HDAC1 signaling.

Background: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible airflow limitation and chronic airway inflammation. Obstructive sleep apnea (OSA) is a common clinical condition with partial or complete pharyngeal collapses occurring repeatedly during sleep. COPD-OSA is the coexistence of COPD and OSA.

Sex-Specific Association of Left Ventricular Hypertrophy With Cardiovascular Events in High-Risk of Cardiovascular Disease Population: Findings From the Sub-Cohort of China PEACE Million Persons Project.

Sex differences significantly influence the prognosis of left ventricular hypertrophy (LVH). To investigate sex-specific differences in the incidence of major adverse cardiovascular event (MACE) among individuals with LVH, we enrolled 14 636 (mean age 57 years, women 59.1%) participants with high risk for cardiovascular diseases (CVD) from the sub-cohort of the China PEACE Million Persons Project.

LncRNA HCG18 regulates the progression of spinal tuberculosis by modulating the hsa-miR-146a-5p/TGF-β1/SMADs pathway.

Spinal tuberculosis is the most common extrapulmonary tuberculosis, characterized by intervertebral disc destruction, which seriously affects people's quality of life. Recent studies have suggested that the TGF-β1/SMADs signaling pathway plays an important regulatory role in the process of intervertebral disc destruction caused by spinal tuberculosis. However, the abnormal TGF-β1/SMADs signaling pathway in spinal tuberculosis is not fully understood.

Effects of Porphyromonas gingivalis lipopolysaccharide on the behavior of human dental pulp stem cells in vitro and their inflammation-related transcriptomics profile.

Objective: The inflammatory microenvironment in pulpitis and periapical periodontitis critically impairs dental pulp stem cells (DPSCs) functionality, yet the underlying molecular mechanisms remain poorly defined. This study aimed to investigate the effects of Porphyromonas gingivalis lipopolysaccharide (P. gingivalis LPS) on human DPSCs behavior and elucidate the transcriptomic changes underlying LPS-induced inflammation.

Biomechanical properties of off-axis screw in the treatment of vertical femoral neck fractures: a finite element analysis.

Purpose: This study aims to assess the biomechanical performance of the 7.3 mm fully threaded off-axis screw (7.3-FTOS) configuration in stabilizing vertical femoral neck fractures (FNSs), comparing its effectiveness with other fixation methods.

Correlation Between Bone Mineral Density And Different Types of Modic Changes in Lumbar Spine.

Introduction: Modic changes (MCs) are a common manifestation of lumbar degenerative disease, classified into three types. However, the relationship between Bone Mineral Density (BMD) and each type of MC at the vertebral lesion sites remains unclear.

Methods: This study included 144 patients who had both lumbar MR and CT images.

Single-cell RNA sequencing highlights a significant retinal Müller glial population in dry age-related macular degeneration.

The main challenge in dissecting the cells and pathways involved in the pathogenesis of age-related macular degeneration (AMD) is the highly heterogeneous and dynamic nature of the retinal microenvironment. This study aimed to describe the comprehensive landscape of the dry AMD (dAMD) model and identify the key cell cluster contributing to dAMD. We identified a subset of Müller cells that express high levels of Sox2, which play crucial roles in homeostasis and neuroprotection in both mouse models of AMD and patients with dAMD.

Experimental study on reconstructing mandibular defects using porous titanium in goats.

This research aimed to explore the effects of varying porosities and pore sizes in porous titanium on osteointegration following mandibular defect reconstruction in goats. Porous titanium products were fabricated using spark plasma sintering technology combined with powder metallurgy techniques. Based on experimental requirements, standard porous titanium samples were categorized into three groups: Group A with 50%-70% porosity and 100-300 μm pore size; Group B with 70%-85% porosity and 300-500 μm pore size; and Group C with no porosity.

Stress hyperglycemia ratio as a predictor of acute kidney injury and its outcomes in critically ill patients.

This study investigated stress hyperglycemia ratio (SHR) for acute kidney injury (AKI) and clinical outcomes in intensive care unit (ICU). Key outcomes were AKI within 48 h after ICU admission, acute kidney disease (AKD), ICU mortality, 28-day mortality, 90-day mortality and 1-year mortality. The associations between SHR and outcomes was estimated logistic regression, Cox proportional hazards regression, and restricted cubic spline (RCS) analyses.

Urinary Cytokeratin 20 Predicts Severe Acute Kidney Injury and Major Adverse Kidney Events in Adults Undergoing Cardiac Surgery.

Introduction: It remains a big challenge to identify patients who are at high risk of developing severe acute kidney injury (AKI) after cardiac surgery. This study investigated the clinical utility of urinary cytokeratin 20 (uCK20), a novel biomarker reflecting severity of histological acute tubular injury, for identifying patients at risk of developing severe AKI.

Methods: This prospective multicenter cohort study enrolled a test set comprising 413 patients who underwent cardiac surgery at 5 centers and a validation set comprising 131 patients at an external center.

Eculizumab as fast-acting rescue therapy for pembrolizumab-induced impending crisis state of myasthenia gravis: a case report.

Immune checkpoint inhibitors, such as pembrolizumab, have demonstrated substantial therapeutic efficacy in the treatment of cancer. However, immune-related adverse events, including myasthenia gravis (MG), present significant clinical challenges. This study presents the case of a 75-year-old male patient who developed generalized acetylcholine receptor antibody-positive MG following the first infusion of pembrolizumab for cholangiocarcinoma.

SMC4 Promotes Prostate Cancer Cell Proliferation and Metastasis via the Rheb/mTOR Pathway.

Structural maintenance of chromosome protein 4 (SMC4), is a key structural component of mitotic chromosomes. While existing evidence indicates a plausible link between SMC4 and oncogenic manifestations, its precise role in the trajectory of prostate cancer remains ambiguous. The Cancer Genome Atlas (TCGA) database analysis reveals that aberrant expression of SMC4 exhibits a robust prognostic association with metastatic progression.

Metabolic Profiling Reveals Diagnostic Biomarkers for Distinguishing Myocarditis From Acute Myocardial Infarction.

Distinguishing between myocarditis (MC) and acute myocardial infarction (AMI) in the early stages is crucial due to their similar symptoms yet vastly different treatment protocols. This study seeks to utilize metabolomics techniques to differentiate between MC and AMI. Plasma samples from 15 patients with MC and 12 patients with AMI were collected.

Silencing GGH induces autophagy by increasing folate stress and production of NADH.

There is an inextricable link between metabolic disorders and autophagy. Gamma-glutamyl hydrolase (GGH) is a lysosomal glycoprotein that reduces intracellular folate stress by catalyzing the hydrolysis of polyglutamylated folate into transportable monoglutamate. The relationship between folate metabolism, involving the folate metabolic enzyme GGH, and autophagy has rarely been reported.

Macrophage polarization regulates the pathogenesis and progression of autoimmune diseases.

Macrophages are integral components of the innate immune system, present in nearly all tissues and organs throughout the body. They exhibit a high degree of plasticity and heterogeneity, participating in immune responses to maintain immune homeostasis. When the immune system loses tolerance, macrophages rapidly proliferate and polarize in response to various signaling pathways within a disrupted microenvironment.